HGEx-ERdb: user guide

HGEx-Erdb can be queried for:

• Phase of menstrual cycle or condition
• Specific gene

Figure A. Search options for querying HGEx-ERdb

a) Search by phase of menstrual cycle and condition
The database can be queried for any phase of the menstrual cycle viz secretory, proliferative and menstrual phase and its stages further to the level of specific days (Figure B,C).
The condition can be selected by clicking on the drop down menu given under conditions (Figure D). Click "Add Selection" and "Submit" to query the selected set of condition (Figure E).
Only simple status can be obtained for a single set of condition. To obtain "Differential status", at least two sets of conditions should be selected. Here, "Both" can be selected (Figure F) to obtain both single and differential status [Figure G(a) and G(b)].
By clicking on a gene in the result page, it connects to the gene information page that has basic information on the gene and links to other information such as protein, transcript, promoter, expression, GO/PPI, PubMed and cross reference (Figures H-O).

b) Search by genes
Enter the gene of interest and select the identifier corresponding to the gene entered (Figure P). Information (Figures H-O) on the gene can be obtained by clicking on the links under the "Information" section (Figure Q).
"Browse" section allows to obtain the information (similar to that obtained from querying genes/conditions) either by clicking genes from the alphabetical list or list of conditions (Figures Q & R).

Figure B. Step I : select the phase of menstrual cycle from the drop down menu.

Figure C. Step II: select the stage of the phase of interest from the drop down menu. Further selection of specific days of the cycle can be done by clicking on the next drop down menu.

Figure D. Step III: select the condition of the phase of interest from the drop down menu.

Figure E. Step IV: add selection and submit to obtain results.

Figure F. Step V: to obtain differential status, add another set of condition and select Differential status to obtain only up and down regulated list OR select Both to get transcribed /dormant and up/down regulated list.

Figure G (a). Result page for expressed . dormant expression for single set of condition.

Figure G (b). Result page for differential expression for two sets of condition

Figure H. Gene information.

Figure I. Protein information.

Figure J. Promoter information.

Figure K. Transcript information.

Figure L. Expression information.

Figure M. Gene Ontology information.

Figure N. PubMed citations for the gene.

Figure O. Cross references for the gene in other databases.

Figure P. Enter the correct identifier to obtain information on the gene of interest.

Figure Q. Select the information required for the queried gene.

Figure R (a). Browse by gene name.

Figure R (b). Browse by conditions.

Figure S. Links to existing Uterus databases.