Search results for GSM147363 ![]() | |
GSM ID | GSM147363 |
GSE ID | GSE6400
|
GPL ID | GPL570 |
Title | Actinomycin D treated A549 human lung cancer cell cultures replicate B |
Source name | Actinomycin D treated A549 human lung cancer cell cultures replicate B |
Characteristics | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated. |
Description | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated. |