Search results for GSM147521 ![]() | |
GSM ID | GSM147521 |
GSE ID | GSE6400
|
GPL ID | GPL570 |
Title | 2.5uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate B |
Source name | 2.5uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate B |
Characteristics | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated. |
Description | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated. |