Search results for GSM310905  | |
| GSM ID | GSM310905 |
| GSE ID | GSE12396
|
| GPL ID | GPL96 |
| Title | Control LXIDN transduced CD34++ cells |
| Source name | CB CD34+ progenitors cells |
| Characteristics | CD34+ hematopoietic progenitors purified from cord blood (CB). Human CD34+ cells were purified from umbilical cord blood samples, collected after normal deliveries, according to the institutional guidelines for discarded material. Mononuclear cells were isolated by Ficoll-Hypaque (Lymphoprep; Nycomed Pharma, Oslo, Norway) gradient separation, washed twice with PBS, and then CD34+ cells separated using magnetic cell sorting procedure (EasySep Human CD34+ positive selection kit, StemCell Techonologies Inc.; Vancouver, Canada). CD34+ cell purity assessed by flow cytometry were always >95%. |
| Description | Synthesis of biotinylated cRNA targets, arrays hybridization (Mouse430_2 GeneChip, Affymetrix, Santa Clara,CA), staining and scanning were performed using Affymetrix standard protocols starting from 100 ng of total RNA. |