Search results for the GEO ID: GSE10070  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM254523 | GPL570 |
|
MCF10A_Monolayer_1
|
monolayer
|
MCF10A cells grown to confluency on sterile plastic from Corning Inc
|
MCF10A_Monolayer_1
FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
|
| Sample_geo_accession | GSM254523
| | Sample_status | Public on Jan 31 2008
| | Sample_submission_date | Jan 04 2008
| | Sample_last_update_date | Jan 07 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal growth media for MCF10As is DMEM:F12 (Cellgro) w/ 2mM glutamine, containing 5% horse serum, insulin (10 µg ml-1) (Gibco), hydrocortisone (0.5 µg ml-1) (Sigma), EGF (20 ng ml-1) (Upstate) and 1 I.U. ml-1 penicillin, 0.1 µg ml-1 streptomysin, 0.25 µg ml-1 amphotericin B (Cellgro).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from cells grown in monolayer and Transwell® cultures were extracted using Tri reagent (MRC, Cincinnati, OH) and cleaned using DNaseI (Promega, Madison, WI) followed by standard phenol/chloroform precipitation and extraction method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Quality of total RNA was first analyzed by Agilent Bioanalyzer 2100, and RNA samples with RIN (RNA Integrity Number) greater than 7.0 are acceptable for microarray analysis. Total RNA samples were amplified one round, and the amplified RNA (aRNA) biotinylated using Ambion Biotin-Enhanced Message Amp II kit (#1791, Ambion, Austin, Texas), following the manufacturer’s protocols.
| | Sample_hyb_protocol | The hybridization, staining, and washing are carried out using the Affymetrix GeneChip Hybridization Wash and Stain Kit following the manufacturer’s protocols, and the arrays were hybridized for 16 hr at 45oC. FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
| | Sample_scan_protocol | The GeneChips are scanned with Affymetrix GeneChip Scanner 3000 7G using GCOS and software and Affymetrix preset settings.
| | Sample_data_processing | Analysis was performed using R statistical software and the limma Bioconductor package 39. All steps of data preprocessing, including background correction, normalization, and expression set summaries, were performed using RMA (Robust Multi-chip Average). Estimated fold changes at each time point were calculated using ANOVA, and resulting t-statistics from each comparison were modified using an intensity-based empirical Bayes method (IBMT) 40 to obtain accurate significance levels. P-values were adjusted using the False Discovery Rate (FDR) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nelson,D,Horseman
| | Sample_contact_email | nelson.horseman@uc.edu
| | Sample_contact_department | Molec & Cell Physiology
| | Sample_contact_institute | University of Cincinnati
| | Sample_contact_address | 231 Albert Sabin Way; MSB 3160
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45267-0576
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254523/suppl/GSM254523.CEL.gz
| | Sample_series_id | GSE10070
| | Sample_data_row_count | 54675
| | |
|
GSM254524 | GPL570 |
|
MCF10A_Monolayer_2
|
monolayer
|
MCF10A cells grown to confluency on sterile plastic from Corning Inc
|
MCF10A_Monolayer_2
FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
|
| Sample_geo_accession | GSM254524
| | Sample_status | Public on Jan 31 2008
| | Sample_submission_date | Jan 04 2008
| | Sample_last_update_date | Jan 07 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal growth media for MCF10As is DMEM:F12 (Cellgro) w/ 2mM glutamine, containing 5% horse serum, insulin (10 µg ml-1) (Gibco), hydrocortisone (0.5 µg ml-1) (Sigma), EGF (20 ng ml-1) (Upstate) and 1 I.U. ml-1 penicillin, 0.1 µg ml-1 streptomysin, 0.25 µg ml-1 amphotericin B (Cellgro).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from cells grown in monolayer and Transwell® cultures were extracted using Tri reagent (MRC, Cincinnati, OH) and cleaned using DNaseI (Promega, Madison, WI) followed by standard phenol/chloroform precipitation and extraction method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Quality of total RNA was first analyzed by Agilent Bioanalyzer 2100, and RNA samples with RIN (RNA Integrity Number) greater than 7.0 are acceptable for microarray analysis. Total RNA samples were amplified one round, and the amplified RNA (aRNA) biotinylated using Ambion Biotin-Enhanced Message Amp II kit (#1791, Ambion, Austin, Texas), following the manufacturer’s protocols.
| | Sample_hyb_protocol | The hybridization, staining, and washing are carried out using the Affymetrix GeneChip Hybridization Wash and Stain Kit following the manufacturer’s protocols, and the arrays were hybridized for 16 hr at 45oC. FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
| | Sample_scan_protocol | The GeneChips are scanned with Affymetrix GeneChip Scanner 3000 7G using GCOS and software and Affymetrix preset settings.
| | Sample_data_processing | Analysis was performed using R statistical software and the limma Bioconductor package 39. All steps of data preprocessing, including background correction, normalization, and expression set summaries, were performed using RMA (Robust Multi-chip Average). Estimated fold changes at each time point were calculated using ANOVA, and resulting t-statistics from each comparison were modified using an intensity-based empirical Bayes method (IBMT) 40 to obtain accurate significance levels. P-values were adjusted using the False Discovery Rate (FDR) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nelson,D,Horseman
| | Sample_contact_email | nelson.horseman@uc.edu
| | Sample_contact_department | Molec & Cell Physiology
| | Sample_contact_institute | University of Cincinnati
| | Sample_contact_address | 231 Albert Sabin Way; MSB 3160
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45267-0576
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254524/suppl/GSM254524.CEL.gz
| | Sample_series_id | GSE10070
| | Sample_data_row_count | 54675
| | |
|
GSM254525 | GPL570 |
|
MCF10A_Monolayer_3
|
monolayer
|
MCF10A cells grown to confluency on sterile plastic from Corning Inc
|
MCF10A_Monolayer_3
FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
|
| Sample_geo_accession | GSM254525
| | Sample_status | Public on Jan 31 2008
| | Sample_submission_date | Jan 04 2008
| | Sample_last_update_date | Apr 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal growth media for MCF10As is DMEM:F12 (Cellgro) w/ 2mM glutamine, containing 5% horse serum, insulin (10 µg ml-1) (Gibco), hydrocortisone (0.5 µg ml-1) (Sigma), EGF (20 ng ml-1) (Upstate) and 1 I.U. ml-1 penicillin, 0.1 µg ml-1 streptomysin, 0.25 µg ml-1 amphotericin B (Cellgro).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from cells grown in monolayer and Transwell® cultures were extracted using Tri reagent (MRC, Cincinnati, OH) and cleaned using DNaseI (Promega, Madison, WI) followed by standard phenol/chloroform precipitation and extraction method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Quality of total RNA was first analyzed by Agilent Bioanalyzer 2100, and RNA samples with RIN (RNA Integrity Number) greater than 7.0 are acceptable for microarray analysis. Total RNA samples were amplified one round, and the amplified RNA (aRNA) biotinylated using Ambion Biotin-Enhanced Message Amp II kit (#1791, Ambion, Austin, Texas), following the manufacturer’s protocols.
| | Sample_hyb_protocol | The hybridization, staining, and washing are carried out using the Affymetrix GeneChip Hybridization Wash and Stain Kit following the manufacturer’s protocols, and the arrays were hybridized for 16 hr at 45oC. FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
| | Sample_scan_protocol | The GeneChips are scanned with Affymetrix GeneChip Scanner 3000 7G using GCOS and software and Affymetrix preset settings.
| | Sample_data_processing | Analysis was performed using R statistical software and the limma Bioconductor package 39. All steps of data preprocessing, including background correction, normalization, and expression set summaries, were performed using RMA (Robust Multi-chip Average). Estimated fold changes at each time point were calculated using ANOVA, and resulting t-statistics from each comparison were modified using an intensity-based empirical Bayes method (IBMT) 40 to obtain accurate significance levels. P-values were adjusted using the False Discovery Rate (FDR) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nelson,D,Horseman
| | Sample_contact_email | nelson.horseman@uc.edu
| | Sample_contact_department | Molec & Cell Physiology
| | Sample_contact_institute | University of Cincinnati
| | Sample_contact_address | 231 Albert Sabin Way; MSB 3160
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45267-0576
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254525/suppl/GSM254525.CEL.gz
| | Sample_relation | Reanalyzed by: GSE17768
| | Sample_relation | Reanalyzed by: GSE21541
| | Sample_relation | Reanalyzed by: GSM533387
| | Sample_series_id | GSE10070
| | Sample_data_row_count | 54675
| | |
|
GSM254538 | GPL570 |
|
MCF10A_Transwell-Base_1
|
Base
|
MCF10A cells were plated onto polyester permeable supports from Corning (Transwells) at a density of 10^5 cells/cm^2. Base represents those with a low trans-epithelial electrical resistance (200-300 Ohms x cm^2)
|
MCF10A_Transwell-Base_1
FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
|
| Sample_geo_accession | GSM254538
| | Sample_status | Public on Jan 31 2008
| | Sample_submission_date | Jan 04 2008
| | Sample_last_update_date | Jan 07 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal growth media for MCF10As is DMEM:F12 (Cellgro) w/ 2mM glutamine, containing 5% horse serum, insulin (10 µg ml-1) (Gibco), hydrocortisone (0.5 µg ml-1) (Sigma), EGF (20 ng ml-1) (Upstate) and 1 I.U. ml-1 penicillin, 0.1 µg ml-1 streptomysin, 0.25 µg ml-1 amphotericin B (Cellgro).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from cells grown in monolayer and Transwell® cultures were extracted using Tri reagent (MRC, Cincinnati, OH) and cleaned using DNaseI (Promega, Madison, WI) followed by standard phenol/chloroform precipitation and extraction method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Quality of total RNA was first analyzed by Agilent Bioanalyzer 2100, and RNA samples with RIN (RNA Integrity Number) greater than 7.0 are acceptable for microarray analysis. Total RNA samples were amplified one round, and the amplified RNA (aRNA) biotinylated using Ambion Biotin-Enhanced Message Amp II kit (#1791, Ambion, Austin, Texas), following the manufacturer’s protocols.
| | Sample_hyb_protocol | The hybridization, staining, and washing are carried out using the Affymetrix GeneChip Hybridization Wash and Stain Kit following the manufacturer’s protocols, and the arrays were hybridized for 16 hr at 45oC. FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
| | Sample_scan_protocol | The GeneChips are scanned with Affymetrix GeneChip Scanner 3000 7G using GCOS and software and Affymetrix preset settings.
| | Sample_data_processing | Analysis was performed using R statistical software and the limma Bioconductor package 39. All steps of data preprocessing, including background correction, normalization, and expression set summaries, were performed using RMA (Robust Multi-chip Average). Estimated fold changes at each time point were calculated using ANOVA, and resulting t-statistics from each comparison were modified using an intensity-based empirical Bayes method (IBMT) 40 to obtain accurate significance levels. P-values were adjusted using the False Discovery Rate (FDR) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nelson,D,Horseman
| | Sample_contact_email | nelson.horseman@uc.edu
| | Sample_contact_department | Molec & Cell Physiology
| | Sample_contact_institute | University of Cincinnati
| | Sample_contact_address | 231 Albert Sabin Way; MSB 3160
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45267-0576
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254538/suppl/GSM254538.CEL.gz
| | Sample_series_id | GSE10070
| | Sample_data_row_count | 54675
| | |
|
GSM254539 | GPL570 |
|
MCF10A_Transwell-Base_2
|
Base
|
MCF10A cells were plated onto polyester permeable supports from Corning (Transwells) at a density of 10^5 cells/cm^2. Base represents those with a low trans-epithelial electrical resistance (200-300 Ohms x cm^2)
|
MCF10A_Transwell-Base_2
FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
|
| Sample_geo_accession | GSM254539
| | Sample_status | Public on Jan 31 2008
| | Sample_submission_date | Jan 04 2008
| | Sample_last_update_date | Jan 07 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal growth media for MCF10As is DMEM:F12 (Cellgro) w/ 2mM glutamine, containing 5% horse serum, insulin (10 µg ml-1) (Gibco), hydrocortisone (0.5 µg ml-1) (Sigma), EGF (20 ng ml-1) (Upstate) and 1 I.U. ml-1 penicillin, 0.1 µg ml-1 streptomysin, 0.25 µg ml-1 amphotericin B (Cellgro).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from cells grown in monolayer and Transwell® cultures were extracted using Tri reagent (MRC, Cincinnati, OH) and cleaned using DNaseI (Promega, Madison, WI) followed by standard phenol/chloroform precipitation and extraction method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Quality of total RNA was first analyzed by Agilent Bioanalyzer 2100, and RNA samples with RIN (RNA Integrity Number) greater than 7.0 are acceptable for microarray analysis. Total RNA samples were amplified one round, and the amplified RNA (aRNA) biotinylated using Ambion Biotin-Enhanced Message Amp II kit (#1791, Ambion, Austin, Texas), following the manufacturer’s protocols.
| | Sample_hyb_protocol | The hybridization, staining, and washing are carried out using the Affymetrix GeneChip Hybridization Wash and Stain Kit following the manufacturer’s protocols, and the arrays were hybridized for 16 hr at 45oC. FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
| | Sample_scan_protocol | The GeneChips are scanned with Affymetrix GeneChip Scanner 3000 7G using GCOS and software and Affymetrix preset settings.
| | Sample_data_processing | Analysis was performed using R statistical software and the limma Bioconductor package 39. All steps of data preprocessing, including background correction, normalization, and expression set summaries, were performed using RMA (Robust Multi-chip Average). Estimated fold changes at each time point were calculated using ANOVA, and resulting t-statistics from each comparison were modified using an intensity-based empirical Bayes method (IBMT) 40 to obtain accurate significance levels. P-values were adjusted using the False Discovery Rate (FDR) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nelson,D,Horseman
| | Sample_contact_email | nelson.horseman@uc.edu
| | Sample_contact_department | Molec & Cell Physiology
| | Sample_contact_institute | University of Cincinnati
| | Sample_contact_address | 231 Albert Sabin Way; MSB 3160
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45267-0576
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254539/suppl/GSM254539.CEL.gz
| | Sample_series_id | GSE10070
| | Sample_data_row_count | 54675
| | |
|
GSM254614 | GPL570 |
|
MCF10A_Transwell-Base_3
|
Base
|
MCF10A cells were plated onto polyester permeable supports from Corning (Transwells) at a density of 10^5 cells/cm^2. Base represents those with a low trans-epithelial electrical resistance (200-300 Ohms x cm^2)
|
MCF10A_Transwell-Base_3
FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
|
| Sample_geo_accession | GSM254614
| | Sample_status | Public on Jan 31 2008
| | Sample_submission_date | Jan 04 2008
| | Sample_last_update_date | Jan 07 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal growth media for MCF10As is DMEM:F12 (Cellgro) w/ 2mM glutamine, containing 5% horse serum, insulin (10 µg ml-1) (Gibco), hydrocortisone (0.5 µg ml-1) (Sigma), EGF (20 ng ml-1) (Upstate) and 1 I.U. ml-1 penicillin, 0.1 µg ml-1 streptomysin, 0.25 µg ml-1 amphotericin B (Cellgro).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from cells grown in monolayer and Transwell® cultures were extracted using Tri reagent (MRC, Cincinnati, OH) and cleaned using DNaseI (Promega, Madison, WI) followed by standard phenol/chloroform precipitation and extraction method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Quality of total RNA was first analyzed by Agilent Bioanalyzer 2100, and RNA samples with RIN (RNA Integrity Number) greater than 7.0 are acceptable for microarray analysis. Total RNA samples were amplified one round, and the amplified RNA (aRNA) biotinylated using Ambion Biotin-Enhanced Message Amp II kit (#1791, Ambion, Austin, Texas), following the manufacturer’s protocols.
| | Sample_hyb_protocol | The hybridization, staining, and washing are carried out using the Affymetrix GeneChip Hybridization Wash and Stain Kit following the manufacturer’s protocols, and the arrays were hybridized for 16 hr at 45oC. FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
| | Sample_scan_protocol | The GeneChips are scanned with Affymetrix GeneChip Scanner 3000 7G using GCOS and software and Affymetrix preset settings.
| | Sample_data_processing | Analysis was performed using R statistical software and the limma Bioconductor package 39. All steps of data preprocessing, including background correction, normalization, and expression set summaries, were performed using RMA (Robust Multi-chip Average). Estimated fold changes at each time point were calculated using ANOVA, and resulting t-statistics from each comparison were modified using an intensity-based empirical Bayes method (IBMT) 40 to obtain accurate significance levels. P-values were adjusted using the False Discovery Rate (FDR) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nelson,D,Horseman
| | Sample_contact_email | nelson.horseman@uc.edu
| | Sample_contact_department | Molec & Cell Physiology
| | Sample_contact_institute | University of Cincinnati
| | Sample_contact_address | 231 Albert Sabin Way; MSB 3160
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45267-0576
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254614/suppl/GSM254614.CEL.gz
| | Sample_series_id | GSE10070
| | Sample_data_row_count | 54675
| | |
|
GSM254615 | GPL570 |
|
MCF10A_Transwell-Midpoint_1
|
Midpoint
|
MCF10A cells were plated onto polyester permeable supports from Corning (Transwells) at a density of 10^5 cells/cm^2. Midpoint represents those with a mid trans-epithelial electrical resistance (1400-1600 Ohms x cm^2)
|
MCF10A_Transwell-Midpoint_1
FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
|
| Sample_geo_accession | GSM254615
| | Sample_status | Public on Jan 31 2008
| | Sample_submission_date | Jan 04 2008
| | Sample_last_update_date | Jan 07 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal growth media for MCF10As is DMEM:F12 (Cellgro) w/ 2mM glutamine, containing 5% horse serum, insulin (10 µg ml-1) (Gibco), hydrocortisone (0.5 µg ml-1) (Sigma), EGF (20 ng ml-1) (Upstate) and 1 I.U. ml-1 penicillin, 0.1 µg ml-1 streptomysin, 0.25 µg ml-1 amphotericin B (Cellgro).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from cells grown in monolayer and Transwell® cultures were extracted using Tri reagent (MRC, Cincinnati, OH) and cleaned using DNaseI (Promega, Madison, WI) followed by standard phenol/chloroform precipitation and extraction method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Quality of total RNA was first analyzed by Agilent Bioanalyzer 2100, and RNA samples with RIN (RNA Integrity Number) greater than 7.0 are acceptable for microarray analysis. Total RNA samples were amplified one round, and the amplified RNA (aRNA) biotinylated using Ambion Biotin-Enhanced Message Amp II kit (#1791, Ambion, Austin, Texas), following the manufacturer’s protocols.
| | Sample_hyb_protocol | The hybridization, staining, and washing are carried out using the Affymetrix GeneChip Hybridization Wash and Stain Kit following the manufacturer’s protocols, and the arrays were hybridized for 16 hr at 45oC. FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
| | Sample_scan_protocol | The GeneChips are scanned with Affymetrix GeneChip Scanner 3000 7G using GCOS and software and Affymetrix preset settings.
| | Sample_data_processing | Analysis was performed using R statistical software and the limma Bioconductor package 39. All steps of data preprocessing, including background correction, normalization, and expression set summaries, were performed using RMA (Robust Multi-chip Average). Estimated fold changes at each time point were calculated using ANOVA, and resulting t-statistics from each comparison were modified using an intensity-based empirical Bayes method (IBMT) 40 to obtain accurate significance levels. P-values were adjusted using the False Discovery Rate (FDR) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nelson,D,Horseman
| | Sample_contact_email | nelson.horseman@uc.edu
| | Sample_contact_department | Molec & Cell Physiology
| | Sample_contact_institute | University of Cincinnati
| | Sample_contact_address | 231 Albert Sabin Way; MSB 3160
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45267-0576
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254615/suppl/GSM254615.CEL.gz
| | Sample_series_id | GSE10070
| | Sample_data_row_count | 54675
| | |
|
GSM254616 | GPL570 |
|
MCF10A_Transwell-Midpoint_2
|
Midpoint
|
MCF10A cells were plated onto polyester permeable supports from Corning (Transwells) at a density of 10^5 cells/cm^2. Midpoint represents those with a mid trans-epithelial electrical resistance (1400-1600 Ohms x cm^2)
|
MCF10A_Transwell-Midpoint_2
FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
|
| Sample_geo_accession | GSM254616
| | Sample_status | Public on Jan 31 2008
| | Sample_submission_date | Jan 04 2008
| | Sample_last_update_date | Jan 07 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal growth media for MCF10As is DMEM:F12 (Cellgro) w/ 2mM glutamine, containing 5% horse serum, insulin (10 µg ml-1) (Gibco), hydrocortisone (0.5 µg ml-1) (Sigma), EGF (20 ng ml-1) (Upstate) and 1 I.U. ml-1 penicillin, 0.1 µg ml-1 streptomysin, 0.25 µg ml-1 amphotericin B (Cellgro).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from cells grown in monolayer and Transwell® cultures were extracted using Tri reagent (MRC, Cincinnati, OH) and cleaned using DNaseI (Promega, Madison, WI) followed by standard phenol/chloroform precipitation and extraction method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Quality of total RNA was first analyzed by Agilent Bioanalyzer 2100, and RNA samples with RIN (RNA Integrity Number) greater than 7.0 are acceptable for microarray analysis. Total RNA samples were amplified one round, and the amplified RNA (aRNA) biotinylated using Ambion Biotin-Enhanced Message Amp II kit (#1791, Ambion, Austin, Texas), following the manufacturer’s protocols.
| | Sample_hyb_protocol | The hybridization, staining, and washing are carried out using the Affymetrix GeneChip Hybridization Wash and Stain Kit following the manufacturer’s protocols, and the arrays were hybridized for 16 hr at 45oC. FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
| | Sample_scan_protocol | The GeneChips are scanned with Affymetrix GeneChip Scanner 3000 7G using GCOS and software and Affymetrix preset settings.
| | Sample_data_processing | Analysis was performed using R statistical software and the limma Bioconductor package 39. All steps of data preprocessing, including background correction, normalization, and expression set summaries, were performed using RMA (Robust Multi-chip Average). Estimated fold changes at each time point were calculated using ANOVA, and resulting t-statistics from each comparison were modified using an intensity-based empirical Bayes method (IBMT) 40 to obtain accurate significance levels. P-values were adjusted using the False Discovery Rate (FDR) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nelson,D,Horseman
| | Sample_contact_email | nelson.horseman@uc.edu
| | Sample_contact_department | Molec & Cell Physiology
| | Sample_contact_institute | University of Cincinnati
| | Sample_contact_address | 231 Albert Sabin Way; MSB 3160
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45267-0576
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254616/suppl/GSM254616.CEL.gz
| | Sample_series_id | GSE10070
| | Sample_data_row_count | 54675
| | |
|
GSM254617 | GPL570 |
|
MCF10A_Transwell-Midpoint_3
|
Midpoint
|
MCF10A cells were plated onto polyester permeable supports from Corning (Transwells) at a density of 10^5 cells/cm^2. Midpoint represents those with a mid trans-epithelial electrical resistance (1400-1600 Ohms x cm^2)
|
MCF10A_Transwell-Midpoint_3
FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
|
| Sample_geo_accession | GSM254617
| | Sample_status | Public on Jan 31 2008
| | Sample_submission_date | Jan 04 2008
| | Sample_last_update_date | Jan 07 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal growth media for MCF10As is DMEM:F12 (Cellgro) w/ 2mM glutamine, containing 5% horse serum, insulin (10 µg ml-1) (Gibco), hydrocortisone (0.5 µg ml-1) (Sigma), EGF (20 ng ml-1) (Upstate) and 1 I.U. ml-1 penicillin, 0.1 µg ml-1 streptomysin, 0.25 µg ml-1 amphotericin B (Cellgro).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from cells grown in monolayer and Transwell® cultures were extracted using Tri reagent (MRC, Cincinnati, OH) and cleaned using DNaseI (Promega, Madison, WI) followed by standard phenol/chloroform precipitation and extraction method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Quality of total RNA was first analyzed by Agilent Bioanalyzer 2100, and RNA samples with RIN (RNA Integrity Number) greater than 7.0 are acceptable for microarray analysis. Total RNA samples were amplified one round, and the amplified RNA (aRNA) biotinylated using Ambion Biotin-Enhanced Message Amp II kit (#1791, Ambion, Austin, Texas), following the manufacturer’s protocols.
| | Sample_hyb_protocol | The hybridization, staining, and washing are carried out using the Affymetrix GeneChip Hybridization Wash and Stain Kit following the manufacturer’s protocols, and the arrays were hybridized for 16 hr at 45oC. FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
| | Sample_scan_protocol | The GeneChips are scanned with Affymetrix GeneChip Scanner 3000 7G using GCOS and software and Affymetrix preset settings.
| | Sample_data_processing | Analysis was performed using R statistical software and the limma Bioconductor package 39. All steps of data preprocessing, including background correction, normalization, and expression set summaries, were performed using RMA (Robust Multi-chip Average). Estimated fold changes at each time point were calculated using ANOVA, and resulting t-statistics from each comparison were modified using an intensity-based empirical Bayes method (IBMT) 40 to obtain accurate significance levels. P-values were adjusted using the False Discovery Rate (FDR) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nelson,D,Horseman
| | Sample_contact_email | nelson.horseman@uc.edu
| | Sample_contact_department | Molec & Cell Physiology
| | Sample_contact_institute | University of Cincinnati
| | Sample_contact_address | 231 Albert Sabin Way; MSB 3160
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45267-0576
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254617/suppl/GSM254617.CEL.gz
| | Sample_series_id | GSE10070
| | Sample_data_row_count | 54675
| | |
|
GSM254618 | GPL570 |
|
MCF10A_Transwell-Plateau_1
|
Plateau
|
MCF10A cells were plated onto polyester permeable supports from Corning (Transwells) at a density of 10^5 cells/cm^2. Plateau represents those with a plateau trans-epithelial electrical resistance (3000-3200 Ohms x cm^2)
|
MCF10A_Transwell-Plateau_1
FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
|
| Sample_geo_accession | GSM254618
| | Sample_status | Public on Jan 31 2008
| | Sample_submission_date | Jan 04 2008
| | Sample_last_update_date | Jan 07 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal growth media for MCF10As is DMEM:F12 (Cellgro) w/ 2mM glutamine, containing 5% horse serum, insulin (10 µg ml-1) (Gibco), hydrocortisone (0.5 µg ml-1) (Sigma), EGF (20 ng ml-1) (Upstate) and 1 I.U. ml-1 penicillin, 0.1 µg ml-1 streptomysin, 0.25 µg ml-1 amphotericin B (Cellgro).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from cells grown in monolayer and Transwell® cultures were extracted using Tri reagent (MRC, Cincinnati, OH) and cleaned using DNaseI (Promega, Madison, WI) followed by standard phenol/chloroform precipitation and extraction method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Quality of total RNA was first analyzed by Agilent Bioanalyzer 2100, and RNA samples with RIN (RNA Integrity Number) greater than 7.0 are acceptable for microarray analysis. Total RNA samples were amplified one round, and the amplified RNA (aRNA) biotinylated using Ambion Biotin-Enhanced Message Amp II kit (#1791, Ambion, Austin, Texas), following the manufacturer’s protocols.
| | Sample_hyb_protocol | The hybridization, staining, and washing are carried out using the Affymetrix GeneChip Hybridization Wash and Stain Kit following the manufacturer’s protocols, and the arrays were hybridized for 16 hr at 45oC. FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
| | Sample_scan_protocol | The GeneChips are scanned with Affymetrix GeneChip Scanner 3000 7G using GCOS and software and Affymetrix preset settings.
| | Sample_data_processing | Analysis was performed using R statistical software and the limma Bioconductor package 39. All steps of data preprocessing, including background correction, normalization, and expression set summaries, were performed using RMA (Robust Multi-chip Average). Estimated fold changes at each time point were calculated using ANOVA, and resulting t-statistics from each comparison were modified using an intensity-based empirical Bayes method (IBMT) 40 to obtain accurate significance levels. P-values were adjusted using the False Discovery Rate (FDR) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nelson,D,Horseman
| | Sample_contact_email | nelson.horseman@uc.edu
| | Sample_contact_department | Molec & Cell Physiology
| | Sample_contact_institute | University of Cincinnati
| | Sample_contact_address | 231 Albert Sabin Way; MSB 3160
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45267-0576
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254618/suppl/GSM254618.CEL.gz
| | Sample_series_id | GSE10070
| | Sample_data_row_count | 54675
| | |
|
GSM254619 | GPL570 |
|
MCF10A_Transwell-Plateau_2
|
Plateau
|
MCF10A cells were plated onto polyester permeable supports from Corning (Transwells) at a density of 10^5 cells/cm^2. Plateau represents those with a plateau trans-epithelial electrical resistance (3000-3200 Ohms x cm^2)
|
MCF10A_Transwell-Plateau_2
FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
|
| Sample_geo_accession | GSM254619
| | Sample_status | Public on Jan 31 2008
| | Sample_submission_date | Jan 04 2008
| | Sample_last_update_date | Jan 07 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal growth media for MCF10As is DMEM:F12 (Cellgro) w/ 2mM glutamine, containing 5% horse serum, insulin (10 µg ml-1) (Gibco), hydrocortisone (0.5 µg ml-1) (Sigma), EGF (20 ng ml-1) (Upstate) and 1 I.U. ml-1 penicillin, 0.1 µg ml-1 streptomysin, 0.25 µg ml-1 amphotericin B (Cellgro).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from cells grown in monolayer and Transwell® cultures were extracted using Tri reagent (MRC, Cincinnati, OH) and cleaned using DNaseI (Promega, Madison, WI) followed by standard phenol/chloroform precipitation and extraction method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Quality of total RNA was first analyzed by Agilent Bioanalyzer 2100, and RNA samples with RIN (RNA Integrity Number) greater than 7.0 are acceptable for microarray analysis. Total RNA samples were amplified one round, and the amplified RNA (aRNA) biotinylated using Ambion Biotin-Enhanced Message Amp II kit (#1791, Ambion, Austin, Texas), following the manufacturer’s protocols.
| | Sample_hyb_protocol | The hybridization, staining, and washing are carried out using the Affymetrix GeneChip Hybridization Wash and Stain Kit following the manufacturer’s protocols, and the arrays were hybridized for 16 hr at 45oC. FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
| | Sample_scan_protocol | The GeneChips are scanned with Affymetrix GeneChip Scanner 3000 7G using GCOS and software and Affymetrix preset settings.
| | Sample_data_processing | Analysis was performed using R statistical software and the limma Bioconductor package 39. All steps of data preprocessing, including background correction, normalization, and expression set summaries, were performed using RMA (Robust Multi-chip Average). Estimated fold changes at each time point were calculated using ANOVA, and resulting t-statistics from each comparison were modified using an intensity-based empirical Bayes method (IBMT) 40 to obtain accurate significance levels. P-values were adjusted using the False Discovery Rate (FDR) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nelson,D,Horseman
| | Sample_contact_email | nelson.horseman@uc.edu
| | Sample_contact_department | Molec & Cell Physiology
| | Sample_contact_institute | University of Cincinnati
| | Sample_contact_address | 231 Albert Sabin Way; MSB 3160
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45267-0576
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254619/suppl/GSM254619.CEL.gz
| | Sample_series_id | GSE10070
| | Sample_data_row_count | 54675
| | |
|
GSM254620 | GPL570 |
|
MCF10A_Transwell-Plateau_3
|
Plateau
|
MCF10A cells were plated onto polyester permeable supports from Corning (Transwells) at a density of 10^5 cells/cm^2. Plateau represents those with a plateau trans-epithelial electrical resistance (3000-3200 Ohms x cm^2)
|
MCF10A_Transwell-Plateau_3
FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
|
| Sample_geo_accession | GSM254620
| | Sample_status | Public on Jan 31 2008
| | Sample_submission_date | Jan 04 2008
| | Sample_last_update_date | Jan 07 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal growth media for MCF10As is DMEM:F12 (Cellgro) w/ 2mM glutamine, containing 5% horse serum, insulin (10 µg ml-1) (Gibco), hydrocortisone (0.5 µg ml-1) (Sigma), EGF (20 ng ml-1) (Upstate) and 1 I.U. ml-1 penicillin, 0.1 µg ml-1 streptomysin, 0.25 µg ml-1 amphotericin B (Cellgro).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from cells grown in monolayer and Transwell® cultures were extracted using Tri reagent (MRC, Cincinnati, OH) and cleaned using DNaseI (Promega, Madison, WI) followed by standard phenol/chloroform precipitation and extraction method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Quality of total RNA was first analyzed by Agilent Bioanalyzer 2100, and RNA samples with RIN (RNA Integrity Number) greater than 7.0 are acceptable for microarray analysis. Total RNA samples were amplified one round, and the amplified RNA (aRNA) biotinylated using Ambion Biotin-Enhanced Message Amp II kit (#1791, Ambion, Austin, Texas), following the manufacturer’s protocols.
| | Sample_hyb_protocol | The hybridization, staining, and washing are carried out using the Affymetrix GeneChip Hybridization Wash and Stain Kit following the manufacturer’s protocols, and the arrays were hybridized for 16 hr at 45oC. FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
| | Sample_scan_protocol | The GeneChips are scanned with Affymetrix GeneChip Scanner 3000 7G using GCOS and software and Affymetrix preset settings.
| | Sample_data_processing | Analysis was performed using R statistical software and the limma Bioconductor package 39. All steps of data preprocessing, including background correction, normalization, and expression set summaries, were performed using RMA (Robust Multi-chip Average). Estimated fold changes at each time point were calculated using ANOVA, and resulting t-statistics from each comparison were modified using an intensity-based empirical Bayes method (IBMT) 40 to obtain accurate significance levels. P-values were adjusted using the False Discovery Rate (FDR) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nelson,D,Horseman
| | Sample_contact_email | nelson.horseman@uc.edu
| | Sample_contact_department | Molec & Cell Physiology
| | Sample_contact_institute | University of Cincinnati
| | Sample_contact_address | 231 Albert Sabin Way; MSB 3160
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45267-0576
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254620/suppl/GSM254620.CEL.gz
| | Sample_series_id | GSE10070
| | Sample_data_row_count | 54675
| | |
|
GSM254621 | GPL570 |
|
MCF10A_Transwell-Plateau_4
|
Plateau
|
MCF10A cells were plated onto polyester permeable supports from Corning (Transwells) at a density of 10^5 cells/cm^2. Plateau represents those with a plateau trans-epithelial electrical resistance (3000-3200 Ohms x cm^2)
|
MCF10A_Transwell-Plateau_4
FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
|
| Sample_geo_accession | GSM254621
| | Sample_status | Public on Jan 31 2008
| | Sample_submission_date | Jan 04 2008
| | Sample_last_update_date | Jan 07 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal growth media for MCF10As is DMEM:F12 (Cellgro) w/ 2mM glutamine, containing 5% horse serum, insulin (10 µg ml-1) (Gibco), hydrocortisone (0.5 µg ml-1) (Sigma), EGF (20 ng ml-1) (Upstate) and 1 I.U. ml-1 penicillin, 0.1 µg ml-1 streptomysin, 0.25 µg ml-1 amphotericin B (Cellgro).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from cells grown in monolayer and Transwell® cultures were extracted using Tri reagent (MRC, Cincinnati, OH) and cleaned using DNaseI (Promega, Madison, WI) followed by standard phenol/chloroform precipitation and extraction method.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Quality of total RNA was first analyzed by Agilent Bioanalyzer 2100, and RNA samples with RIN (RNA Integrity Number) greater than 7.0 are acceptable for microarray analysis. Total RNA samples were amplified one round, and the amplified RNA (aRNA) biotinylated using Ambion Biotin-Enhanced Message Amp II kit (#1791, Ambion, Austin, Texas), following the manufacturer’s protocols.
| | Sample_hyb_protocol | The hybridization, staining, and washing are carried out using the Affymetrix GeneChip Hybridization Wash and Stain Kit following the manufacturer’s protocols, and the arrays were hybridized for 16 hr at 45oC. FS450_0001 protocol is used for staining and washing the GeneChips using the Affymetrix Fluidics Station 450 (P/N 00-0079).
| | Sample_scan_protocol | The GeneChips are scanned with Affymetrix GeneChip Scanner 3000 7G using GCOS and software and Affymetrix preset settings.
| | Sample_data_processing | Analysis was performed using R statistical software and the limma Bioconductor package 39. All steps of data preprocessing, including background correction, normalization, and expression set summaries, were performed using RMA (Robust Multi-chip Average). Estimated fold changes at each time point were calculated using ANOVA, and resulting t-statistics from each comparison were modified using an intensity-based empirical Bayes method (IBMT) 40 to obtain accurate significance levels. P-values were adjusted using the False Discovery Rate (FDR) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nelson,D,Horseman
| | Sample_contact_email | nelson.horseman@uc.edu
| | Sample_contact_department | Molec & Cell Physiology
| | Sample_contact_institute | University of Cincinnati
| | Sample_contact_address | 231 Albert Sabin Way; MSB 3160
| | Sample_contact_city | Cincinnati
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 45267-0576
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254621/suppl/GSM254621.CEL.gz
| | Sample_series_id | GSE10070
| | Sample_data_row_count | 54675
| | |
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Make groups for comparisons |
(2 groups will be compared at a time) |
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