Search results for the GEO ID: GSE10081  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM254859 | GPL339 |
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embryo, matUPD12, 430A
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15.5 dpc whole embryo, matUPD12
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Strain: offspring of Rb(8.12)5Bnr/Rb(6.12)3Sic double heterozygote females on a BALB/c background crossed with Rb(8.12)5Bnr/Rb(4.12)9Bnr double heterozygote males on a C57BL/6J background (Development 127, 4719-4728 (2000)) with a maternally-derived uniparental disomy of whole Chr 12
Age: 15.5 dpc
Tissue: whole embryo
|
Comparison of gene expression levels between matUPD12 and patUPD12 samples. Identification of highly differentially expressed transcripts. See UPD12 series record.
|
| Sample_geo_accession | GSM254859
| | Sample_status | Public on Apr 14 2008
| | Sample_submission_date | Jan 07 2008
| | Sample_last_update_date | Apr 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Anne C. Ferguson-Smith, Department of Anatomy, University of Cambridge
| | Sample_treatment_protocol_ch1 | Tissues were snap frozen in liquid nitrogen, permanently stored at -80C, and put on dry ice during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen kit according to manufacturers protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was quantified on an Agilent Bioanalyser and 5 to 7µg were used to prepare biotin-labeled cRNA target, as prescribed in the Affymetrix expression manual (Affymetrix, 2004). The biotinylated cRNA target was purified using cRNA Cleanup spin columns (Affymetrix), fragmented with 5x fragmentation buffer (Affymetrix), and quantified prior to hybridization on an Agilent Bioanalyser.
| | Sample_hyb_protocol | 15µg of labeled probe was hybridised to the array, washed and stained on the Affymetrix fluidics station 450. Standard protocol.
| | Sample_scan_protocol | The Affymetrix Scanner 3000 system was used. Standard protocol.
| | Sample_data_processing | MAS5/GCOS: TGT=500, otherwise default values.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Reiner,,Schulz
| | Sample_contact_email | reiner.schulz@genetics.kcl.ac.uk
| | Sample_contact_laboratory | Epigenetics
| | Sample_contact_department | Medical & Molecular Genetics
| | Sample_contact_institute | King's College London
| | Sample_contact_address | 8th floor Guy's Tower
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE1 9RT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254859/suppl/GSM254859.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254859/suppl/GSM254859.CHP.gz
| | Sample_series_id | GSE10081
| | Sample_series_id | GSE10118
| | Sample_data_row_count | 22690
| | |
|
GSM254861 | GPL339 |
|
embryo, patUPD12, 430A
|
15.5 dpc whole embryo, patUPD12
|
Strain: offspring of Rb(8.12)5Bnr/Rb(6.12)3Sic double heterozygote females on a BALB/c background crossed with Rb(8.12)5Bnr/Rb(4.12)9Bnr double heterozygote males on a C57BL/6J background (Development 127, 4719-4728 (2000)) with a paternally-derived uniparental disomy of whole Chr 12
Age: 15.5 dpc
Tissue: whole embryo
|
Comparison of gene expression levels between matUPD12 and patUPD12 samples. Identification of highly differentially expressed transcripts. See UPD12 series record.
|
| Sample_geo_accession | GSM254861
| | Sample_status | Public on Apr 14 2008
| | Sample_submission_date | Jan 07 2008
| | Sample_last_update_date | Apr 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Anne C. Ferguson-Smith, Department of Anatomy, University of Cambridge
| | Sample_treatment_protocol_ch1 | Tissues were snap frozen in liquid nitrogen, permanently stored at -80C, and put on dry ice during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen kit according to manufacturers protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was quantified on an Agilent Bioanalyser and 5 to 7µg were used to prepare biotin-labeled cRNA target, as prescribed in the Affymetrix expression manual (Affymetrix, 2004). The biotinylated cRNA target was purified using cRNA Cleanup spin columns (Affymetrix), fragmented with 5x fragmentation buffer (Affymetrix), and quantified prior to hybridization on an Agilent Bioanalyser.
| | Sample_hyb_protocol | 15µg of labeled probe was hybridised to the array, washed and stained on the Affymetrix fluidics station 450. Standard protocol.
| | Sample_scan_protocol | The Affymetrix Scanner 3000 system was used. Standard protocol.
| | Sample_data_processing | MAS5/GCOS: TGT=500, otherwise default values.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Reiner,,Schulz
| | Sample_contact_email | reiner.schulz@genetics.kcl.ac.uk
| | Sample_contact_laboratory | Epigenetics
| | Sample_contact_department | Medical & Molecular Genetics
| | Sample_contact_institute | King's College London
| | Sample_contact_address | 8th floor Guy's Tower
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE1 9RT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254861/suppl/GSM254861.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254861/suppl/GSM254861.CHP.gz
| | Sample_series_id | GSE10081
| | Sample_series_id | GSE10118
| | Sample_data_row_count | 22690
| | |
|
GSM254863 | GPL82 |
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placenta, matUPD12, U74B
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15.5 dpc placenta, matUPD12
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Strain: offspring of Rb(8.12)5Bnr/Rb(6.12)3Sic double heterozygote females on a BALB/c background crossed with Rb(8.12)5Bnr/Rb(4.12)9Bnr double heterozygote males on a C57BL/6J background (Development 127, 4719-4728 (2000)) with a maternally-derived uniparental disomy of whole Chr 12
Age: 15.5 dpc
Tissue: placenta
|
Comparison of gene expression levels between matUPD12 and patUPD12 samples. Identification of highly differentially expressed transcripts. See UPD12 series record.
|
| Sample_geo_accession | GSM254863
| | Sample_status | Public on Apr 14 2008
| | Sample_submission_date | Jan 07 2008
| | Sample_last_update_date | Apr 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Anne C. Ferguson-Smith, Department of Anatomy, University of Cambridge
| | Sample_treatment_protocol_ch1 | Tissues were snap frozen in liquid nitrogen, permanently stored at -80C, and put on dry ice during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen kit according to manufacturers protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was quantified on an Agilent Bioanalyser and 5 to 7µg were used to prepare biotin-labeled cRNA target, as prescribed in the Affymetrix expression manual (Affymetrix, 2004). The biotinylated cRNA target was purified using cRNA Cleanup spin columns (Affymetrix), fragmented with 5x fragmentation buffer (Affymetrix), and quantified prior to hybridization on an Agilent Bioanalyser.
| | Sample_hyb_protocol | 15µg of labeled probe was hybridised to the array, washed and stained on the Affymetrix fluidics station 450. Standard protocol.
| | Sample_scan_protocol | The Affymetrix Scanner 3000 system was used. Standard protocol.
| | Sample_data_processing | MAS5/GCOS: TGT=500, otherwise default values.
| | Sample_platform_id | GPL82
| | Sample_contact_name | Reiner,,Schulz
| | Sample_contact_email | reiner.schulz@genetics.kcl.ac.uk
| | Sample_contact_laboratory | Epigenetics
| | Sample_contact_department | Medical & Molecular Genetics
| | Sample_contact_institute | King's College London
| | Sample_contact_address | 8th floor Guy's Tower
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE1 9RT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254863/suppl/GSM254863.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254863/suppl/GSM254863.cel.gz
| | Sample_series_id | GSE10081
| | Sample_series_id | GSE10118
| | Sample_data_row_count | 12477
| | |
|
GSM254867 | GPL82 |
|
placenta, patUPD12, U74B
|
15.5 dpc placenta, patUPD12
|
Strain: offspring of Rb(8.12)5Bnr/Rb(6.12)3Sic double heterozygote females on a BALB/c background crossed with Rb(8.12)5Bnr/Rb(4.12)9Bnr double heterozygote males on a C57BL/6J background (Development 127, 4719-4728 (2000)) with a paternally-derived uniparental disomy of whole Chr 12
Age: 15.5 dpc
Tissue: placenta
|
Comparison of gene expression levels between matUPD12 and patUPD12 samples. Identification of highly differentially expressed transcripts. See UPD12 series record.
|
| Sample_geo_accession | GSM254867
| | Sample_status | Public on Apr 14 2008
| | Sample_submission_date | Jan 07 2008
| | Sample_last_update_date | Apr 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Anne C. Ferguson-Smith, Department of Anatomy, University of Cambridge
| | Sample_treatment_protocol_ch1 | Tissues were snap frozen in liquid nitrogen, permanently stored at -80C, and put on dry ice during transport.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen kit according to manufacturers protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Total RNA was quantified on an Agilent Bioanalyser and 5 to 7µg were used to prepare biotin-labeled cRNA target, as prescribed in the Affymetrix expression manual (Affymetrix, 2004). The biotinylated cRNA target was purified using cRNA Cleanup spin columns (Affymetrix), fragmented with 5x fragmentation buffer (Affymetrix), and quantified prior to hybridization on an Agilent Bioanalyser.
| | Sample_hyb_protocol | 15µg of labeled probe was hybridised to the array, washed and stained on the Affymetrix fluidics station 450. Standard protocol.
| | Sample_scan_protocol | The Affymetrix Scanner 3000 system was used. Standard protocol.
| | Sample_data_processing | MAS5/GCOS: TGT=500, otherwise default values.
| | Sample_platform_id | GPL82
| | Sample_contact_name | Reiner,,Schulz
| | Sample_contact_email | reiner.schulz@genetics.kcl.ac.uk
| | Sample_contact_laboratory | Epigenetics
| | Sample_contact_department | Medical & Molecular Genetics
| | Sample_contact_institute | King's College London
| | Sample_contact_address | 8th floor Guy's Tower
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SE1 9RT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254867/suppl/GSM254867.CHP.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM254nnn/GSM254867/suppl/GSM254867.cel.gz
| | Sample_series_id | GSE10081
| | Sample_series_id | GSE10118
| | Sample_data_row_count | 12477
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