Search results for the GEO ID: GSE10097  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM255006 | GPL96 |
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Primary human fetal mixed neuronal/glial cell culture
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Human fetal material, 8-12 weeks, from legal abortions
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Primary cell culture
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Primary mixed neuronal/glial cell cultures were established from human brain tissue, which was obtained from 8-12 weeks old fetuses at legal abortion after informed consent from the patients. Procedures were approved by the local Ethics committee at the Karolinska University Hospital, Stockholm. A total of 23 tissue samples yielding 9 cell cultures were used in this experiment. The cells were harvested after 8 days of culturing for RNA extraction.
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| Sample_geo_accession | GSM255006
| | Sample_status | Public on May 28 2008
| | Sample_submission_date | Jan 08 2008
| | Sample_last_update_date | Apr 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Karolinska University Hospital, Stockholm, Sweden (anonymous donors)
| | Sample_growth_protocol_ch1 | Primary mixed neuronal/glial cell cultures were established from human brain tissue, which was obtained from 8-12 weeks old fetuses at legal abortion after informed consent from the patients. Procedures were approved by the local Ethics committee at the Karolinska University Hospital, Stockholm. The cells were grown on Poly-D-lysine in DMEM medium supplemented with 10% newborn calf serum, L-Glutamine and Antibiotic-antimycotic. The cells were grown for 8 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen RNeasy Mini Kit, followed by further cleaning and concentration by using RNeasy MinElute Cleanup Kit.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recommended protocols.
| | Sample_hyb_protocol | Hybridization was performed at the Cancer Centre Karolinska Microarray Core Facility at the Karolinska University Hospital in Solna according to the manufactureres protocol.
| | Sample_scan_protocol | Scanning was performed according to the manufacturer's recommended protocols with Agilent Probe Array Scan.
| | Sample_data_processing | Raw data was analysed with the MAS5 method using Affymetrix GeneChip Operating Software (GCOS). Transcripts with signal intensities <100 in both samples were excluded.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Linda,Alexandra Csöregh,Nord
| | Sample_contact_email | linda.csoregh@ki.se
| | Sample_contact_department | Dept. of Women's and Children's Health
| | Sample_contact_institute | Karolinska Institute
| | Sample_contact_address | Karolinska University Hospital, Solna, C4:U1
| | Sample_contact_city | Stockholm
| | Sample_contact_zip/postal_code | 171 76
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM255nnn/GSM255006/suppl/GSM255006.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM255nnn/GSM255006/suppl/GSM255006.CHP.gz
| | Sample_series_id | GSE10097
| | Sample_data_row_count | 22283
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GSM255007 | GPL96 |
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Oestrogen treated primary human fetal mixed neuronal/glial cell culture
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Human fetal material, 8-12 weeks, from legal abortions
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Primary cell culture grown in medium containing oestradiol
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Primary mixed neuronal/glial cell cultures were established from human brain tissue, which was obtained from 8-12 weeks old fetuses at legal abortion after informed consent from the patients. A total of 23 tissue samples yielding 9 cell cultures were used in this experiment. Half of the cell cultures were treated with β-oestradiol the day after seeding. The duration of the oestrogen treatment was 7 days and the cells were harvested after 8 days of culturing for RNA extraction.
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| Sample_geo_accession | GSM255007
| | Sample_status | Public on May 28 2008
| | Sample_submission_date | Jan 08 2008
| | Sample_last_update_date | Apr 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Karolinska University Hospital, Stockholm, Sweden (anonymous donors)
| | Sample_treatment_protocol_ch1 | The cell cultures were treated with 2µM β-oestradiol, the day after seeding. Medium was not changed during the culture period. The duration of oestrogen treatment was 7 days and then cells were harvested for RNA extraction.
| | Sample_growth_protocol_ch1 | Primary mixed neuronal/glial cell cultures were established from human brain tissue, which was obtained from 8-12 weeks old fetuses at legal abortion after informed consent from the patients. Procedures were approved by the local Ethics committee at the Karolinska University Hospital, Stockholm. The cells were grown on Poly-D-lysine in DMEM medium supplemented with 10% newborn calf serum, L-Glutamine and Antibiotic-antimycotic. The cells were grown for 8 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen RNeasy Mini Kit, followed by further cleaning and concentration by using RNeasy MinElute Cleanup Kit.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recommended protocols.
| | Sample_hyb_protocol | Hybridization was performed at the Cancer Centre Karolinska Microarray Core Facility at the Karolinska University Hospital in Solna according to the manufactureres protocol.
| | Sample_scan_protocol | Scanning was performed according to the manufacturer's recommended protocols with Agilent Probe Array Scan.
| | Sample_data_processing | Raw data was analysed with the MAS5 method using Affymetrix GeneChip Operating Software (GCOS). Transcripts with signal intensities <100 in both samples were excluded.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Linda,Alexandra Csöregh,Nord
| | Sample_contact_email | linda.csoregh@ki.se
| | Sample_contact_department | Dept. of Women's and Children's Health
| | Sample_contact_institute | Karolinska Institute
| | Sample_contact_address | Karolinska University Hospital, Solna, C4:U1
| | Sample_contact_city | Stockholm
| | Sample_contact_zip/postal_code | 171 76
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM255nnn/GSM255007/suppl/GSM255007.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM255nnn/GSM255007/suppl/GSM255007.CHP.gz
| | Sample_series_id | GSE10097
| | Sample_data_row_count | 22283
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