Search results for the GEO ID: GSE10147 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM256745 | GPL570 |
|
pDC treated with IL-3, biological rep1
|
blood, pDC treated with IL-13
|
Tissue: human plasmacytoid dendritic cells
Treatment: IL-3
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Gene expression data from pDC treated with IL-3
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Sample_geo_accession | GSM256745
| Sample_status | Public on Feb 14 2008
| Sample_submission_date | Jan 11 2008
| Sample_last_update_date | Feb 14 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | pDCs were stimulated with 500 ng/ml of recombinant p17 or 6 µg/ml CpG type A oligonucleotides 2216 (CpG) for 18 hours.
| Sample_growth_protocol_ch1 | pDCs were cultured in 96-well flat bottom plates in RPMI 1640 culture medium supplemented with 10% FCS, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1% nonessential amino acids plus 20 ng/ml IL-3.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen RNA extraction easy kit according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized on Affymetrix HG_U133 plus 2.0 Genechips according to manufacturer's protocol. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner.
| Sample_data_processing | The data were analyzed with RMA.
| Sample_platform_id | GPL570
| Sample_contact_name | Simona,,Fiorentini
| Sample_contact_email | sfiorent@alice.it, sfiorent@med.unibs.it
| Sample_contact_phone | +39-0303996696
| Sample_contact_fax | +39-030-395258
| Sample_contact_laboratory | Section of Microbiology
| Sample_contact_department | Experimental and Applied Medicine
| Sample_contact_institute | University of Brescia
| Sample_contact_address | piazzale Spedali Civili 1
| Sample_contact_city | Brescia
| Sample_contact_zip/postal_code | 25123
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM256nnn/GSM256745/suppl/GSM256745.CEL.gz
| Sample_series_id | GSE10147
| Sample_data_row_count | 54675
| |
|
GSM256746 | GPL570 |
|
pDC treated with IL-3+p17, biological rep1
|
blood, pDC treated with IL-3+p17
|
Tissue: human plasmacytoid dendritic cells
Treatment: IL-3+p17
|
Gene expression data from pDC treated with IL-3+p17
|
Sample_geo_accession | GSM256746
| Sample_status | Public on Feb 14 2008
| Sample_submission_date | Jan 11 2008
| Sample_last_update_date | Feb 14 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | pDCs were stimulated with 500 ng/ml of recombinant p17 or 6 µg/ml CpG type A oligonucleotides 2216 (CpG) for 18 hours.
| Sample_growth_protocol_ch1 | pDCs were cultured in 96-well flat bottom plates in RPMI 1640 culture medium supplemented with 10% FCS, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1% nonessential amino acids plus 20 ng/ml IL-3.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen RNA extraction easy kit according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized on Affymetrix HG_U133 plus 2.0 Genechips according to manufacturer's protocol. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner.
| Sample_data_processing | The data were analyzed with RMA.
| Sample_platform_id | GPL570
| Sample_contact_name | Simona,,Fiorentini
| Sample_contact_email | sfiorent@alice.it, sfiorent@med.unibs.it
| Sample_contact_phone | +39-0303996696
| Sample_contact_fax | +39-030-395258
| Sample_contact_laboratory | Section of Microbiology
| Sample_contact_department | Experimental and Applied Medicine
| Sample_contact_institute | University of Brescia
| Sample_contact_address | piazzale Spedali Civili 1
| Sample_contact_city | Brescia
| Sample_contact_zip/postal_code | 25123
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM256nnn/GSM256746/suppl/GSM256746.CEL.gz
| Sample_series_id | GSE10147
| Sample_data_row_count | 54675
| |
|
GSM256747 | GPL570 |
|
pDC treated with IL-3+CpG, biological rep1
|
blood, pDC treated with IL-3+CpG
|
Tissue: human plasmacytoid dendritic cells
Treatment: IL-3+CpG
|
Gene expression data from pDC treated with IL-3+CpG
|
Sample_geo_accession | GSM256747
| Sample_status | Public on Feb 14 2008
| Sample_submission_date | Jan 11 2008
| Sample_last_update_date | Feb 14 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | pDCs were stimulated with 500 ng/ml of recombinant p17 or 6 µg/ml CpG type A oligonucleotides 2216 (CpG) for 18 hours.
| Sample_growth_protocol_ch1 | pDCs were cultured in 96-well flat bottom plates in RPMI 1640 culture medium supplemented with 10% FCS, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1% nonessential amino acids plus 20 ng/ml IL-3.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen RNA extraction easy kit according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized on Affymetrix HG_U133 plus 2.0 Genechips according to manufacturer's protocol. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner.
| Sample_data_processing | The data were analyzed with RMA.
| Sample_platform_id | GPL570
| Sample_contact_name | Simona,,Fiorentini
| Sample_contact_email | sfiorent@alice.it, sfiorent@med.unibs.it
| Sample_contact_phone | +39-0303996696
| Sample_contact_fax | +39-030-395258
| Sample_contact_laboratory | Section of Microbiology
| Sample_contact_department | Experimental and Applied Medicine
| Sample_contact_institute | University of Brescia
| Sample_contact_address | piazzale Spedali Civili 1
| Sample_contact_city | Brescia
| Sample_contact_zip/postal_code | 25123
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM256nnn/GSM256747/suppl/GSM256747.CEL.gz
| Sample_series_id | GSE10147
| Sample_data_row_count | 54675
| |
|
GSM256748 | GPL570 |
|
pDC treated with IL-3, biological rep2
|
blood, pDC treated with IL-3
|
Tissue: human plasmacytoid dendritic cells
Treatment: IL-3
|
Gene expression data from pDC treated with IL-3
|
Sample_geo_accession | GSM256748
| Sample_status | Public on Feb 14 2008
| Sample_submission_date | Jan 11 2008
| Sample_last_update_date | Feb 14 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | pDCs were stimulated with 500 ng/ml of recombinant p17 or 6 µg/ml CpG type A oligonucleotides 2216 (CpG) for 18 hours.
| Sample_growth_protocol_ch1 | pDCs were cultured in 96-well flat bottom plates in RPMI 1640 culture medium supplemented with 10% FCS, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1% nonessential amino acids plus 20 ng/ml IL-3.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen RNA extraction easy kit according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized on Affymetrix HG_U133 plus 2.0 Genechips according to manufacturer's protocol. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner.
| Sample_data_processing | The data were analyzed with RMA.
| Sample_platform_id | GPL570
| Sample_contact_name | Simona,,Fiorentini
| Sample_contact_email | sfiorent@alice.it, sfiorent@med.unibs.it
| Sample_contact_phone | +39-0303996696
| Sample_contact_fax | +39-030-395258
| Sample_contact_laboratory | Section of Microbiology
| Sample_contact_department | Experimental and Applied Medicine
| Sample_contact_institute | University of Brescia
| Sample_contact_address | piazzale Spedali Civili 1
| Sample_contact_city | Brescia
| Sample_contact_zip/postal_code | 25123
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM256nnn/GSM256748/suppl/GSM256748.CEL.gz
| Sample_series_id | GSE10147
| Sample_data_row_count | 54675
| |
|
GSM256749 | GPL570 |
|
pDC treated with IL-3+p17, biological rep2
|
blood, pDC treated with IL-3+p17
|
Tissue: human plasmacytoid dendritic cells
Treatment: IL-3+p17
|
Gene expression data from pDC treated with IL-3+p17
|
Sample_geo_accession | GSM256749
| Sample_status | Public on Feb 14 2008
| Sample_submission_date | Jan 11 2008
| Sample_last_update_date | Feb 14 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | pDCs were stimulated with 500 ng/ml of recombinant p17 or 6 µg/ml CpG type A oligonucleotides 2216 (CpG) for 18 hours.
| Sample_growth_protocol_ch1 | pDCs were cultured in 96-well flat bottom plates in RPMI 1640 culture medium supplemented with 10% FCS, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1% nonessential amino acids plus 20 ng/ml IL-3.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen RNA extraction easy kit according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized on Affymetrix HG_U133 plus 2.0 Genechips according to manufacturer's protocol. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner.
| Sample_data_processing | The data were analyzed with RMA.
| Sample_platform_id | GPL570
| Sample_contact_name | Simona,,Fiorentini
| Sample_contact_email | sfiorent@alice.it, sfiorent@med.unibs.it
| Sample_contact_phone | +39-0303996696
| Sample_contact_fax | +39-030-395258
| Sample_contact_laboratory | Section of Microbiology
| Sample_contact_department | Experimental and Applied Medicine
| Sample_contact_institute | University of Brescia
| Sample_contact_address | piazzale Spedali Civili 1
| Sample_contact_city | Brescia
| Sample_contact_zip/postal_code | 25123
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM256nnn/GSM256749/suppl/GSM256749.CEL.gz
| Sample_series_id | GSE10147
| Sample_data_row_count | 54675
| |
|
GSM256750 | GPL570 |
|
pDC treated with IL-3+CpG, biological rep2
|
blood, pDC treated with IL-3+CpG
|
Tissue: human plasmacytoid dendritic cells
Treatment: IL-3+CpG
|
Gene expression data from pDC treated with IL-3+CpG
|
Sample_geo_accession | GSM256750
| Sample_status | Public on Feb 14 2008
| Sample_submission_date | Jan 11 2008
| Sample_last_update_date | Feb 14 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | pDCs were stimulated with 500 ng/ml of recombinant p17 or 6 µg/ml CpG type A oligonucleotides 2216 (CpG) for 18 hours.
| Sample_growth_protocol_ch1 | pDCs were cultured in 96-well flat bottom plates in RPMI 1640 culture medium supplemented with 10% FCS, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1% nonessential amino acids plus 20 ng/ml IL-3.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen RNA extraction easy kit according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized on Affymetrix HG_U133 plus 2.0 Genechips according to manufacturer's protocol. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner.
| Sample_data_processing | The data were analyzed with RMA.
| Sample_platform_id | GPL570
| Sample_contact_name | Simona,,Fiorentini
| Sample_contact_email | sfiorent@alice.it, sfiorent@med.unibs.it
| Sample_contact_phone | +39-0303996696
| Sample_contact_fax | +39-030-395258
| Sample_contact_laboratory | Section of Microbiology
| Sample_contact_department | Experimental and Applied Medicine
| Sample_contact_institute | University of Brescia
| Sample_contact_address | piazzale Spedali Civili 1
| Sample_contact_city | Brescia
| Sample_contact_zip/postal_code | 25123
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM256nnn/GSM256750/suppl/GSM256750.CEL.gz
| Sample_series_id | GSE10147
| Sample_data_row_count | 54675
| |
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