Search results for the GEO ID: GSE10196 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM257525 | GPL570 |
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vector control cells, biological rep1
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vector control cells, biological rep1
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MCF10A cells
|
MCF10A cells were infected with retrovirus constructs (vector or YAP) and puromycin was used to select for transduced cells. Cells were split and grown to ~60-75%% confluency at which point they were harvested for RNA.
|
Sample_geo_accession | GSM257525
| Sample_status | Public on Jan 31 2008
| Sample_submission_date | Jan 17 2008
| Sample_last_update_date | Jan 17 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Standard MCF10A growth conditions.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System.
| Sample_data_processing | The data were analyzed with GeneChip Operating Software Ver. 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Daniel,,Haber
| Sample_contact_department | Cancer Center
| Sample_contact_institute | Massachusetts General Hospital
| Sample_contact_address | Rm.7119, Bldg.149, 13th Street
| Sample_contact_city | Charlestown
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02129
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257525/suppl/GSM257525.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257525/suppl/GSM257525.CHP.gz
| Sample_series_id | GSE10196
| Sample_data_row_count | 54675
| |
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GSM257526 | GPL570 |
|
YAP-overexpressing cells, biological rep1
|
YAP-overexpressing cells, biological rep1
|
MCF10A cells
|
MCF10A cells were infected with retrovirus constructs (vector or YAP) and puromycin was used to select for transduced cells. Cells were split and grown to ~60-75%% confluency at which point they were harvested for RNA.
|
Sample_geo_accession | GSM257526
| Sample_status | Public on Jan 31 2008
| Sample_submission_date | Jan 17 2008
| Sample_last_update_date | Jan 17 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Standard MCF10A growth conditions.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System.
| Sample_data_processing | The data were analyzed with GeneChip Operating Software Ver. 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Daniel,,Haber
| Sample_contact_department | Cancer Center
| Sample_contact_institute | Massachusetts General Hospital
| Sample_contact_address | Rm.7119, Bldg.149, 13th Street
| Sample_contact_city | Charlestown
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02129
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257526/suppl/GSM257526.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257526/suppl/GSM257526.CHP.gz
| Sample_series_id | GSE10196
| Sample_data_row_count | 54675
| |
|
GSM257527 | GPL570 |
|
vector control cells, biological rep2
|
vector control cells, biological rep2
|
MCF10A cells
|
MCF10A cells were infected with retrovirus constructs (vector or YAP) and puromycin was used to select for transduced cells. Cells were split and grown to ~60-75%% confluency at which point they were harvested for RNA.
|
Sample_geo_accession | GSM257527
| Sample_status | Public on Jan 31 2008
| Sample_submission_date | Jan 17 2008
| Sample_last_update_date | Jan 17 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Standard MCF10A growth conditions.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System.
| Sample_data_processing | The data were analyzed with GeneChip Operating Software Ver. 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Daniel,,Haber
| Sample_contact_department | Cancer Center
| Sample_contact_institute | Massachusetts General Hospital
| Sample_contact_address | Rm.7119, Bldg.149, 13th Street
| Sample_contact_city | Charlestown
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02129
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257527/suppl/GSM257527.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257527/suppl/GSM257527.CHP.gz
| Sample_series_id | GSE10196
| Sample_data_row_count | 54675
| |
|
GSM257528 | GPL570 |
|
YAP-overexpressing cells, biological rep2
|
YAP-overexpressing cells, biological rep2
|
MCF10A cells
|
MCF10A cells were infected with retrovirus constructs (vector or YAP) and puromycin was used to select for transduced cells. Cells were split and grown to ~60-75%% confluency at which point they were harvested for RNA.
|
Sample_geo_accession | GSM257528
| Sample_status | Public on Jan 31 2008
| Sample_submission_date | Jan 17 2008
| Sample_last_update_date | Jan 17 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Standard MCF10A growth conditions.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNeasy miniprep columns (Qiagen) were used according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G System.
| Sample_data_processing | The data were analyzed with GeneChip Operating Software Ver. 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Daniel,,Haber
| Sample_contact_department | Cancer Center
| Sample_contact_institute | Massachusetts General Hospital
| Sample_contact_address | Rm.7119, Bldg.149, 13th Street
| Sample_contact_city | Charlestown
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02129
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257528/suppl/GSM257528.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257528/suppl/GSM257528.CHP.gz
| Sample_series_id | GSE10196
| Sample_data_row_count | 54675
| |
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