Search results for the GEO ID: GSE10234  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM257640 | GPL570 |
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MM6 cyclin T1 knockdown LPS treated 1
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Mono Mac 6 cells
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Monocytic cell line
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All the above steps after RNA extraction were done by the Microarray Core Facility at Baylor College of Medicine. This sample is numbered as 9548.
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| Sample_geo_accession | GSM257640
| | Sample_status | Public on Jul 31 2008
| | Sample_submission_date | Jan 17 2008
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM6 cells were transduced with shRNA against cyclin T1 and then treated with 10 ng/ml LPS.
| | Sample_growth_protocol_ch1 | MM6 cells were maintained in RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Hyclone), ), non-essential amino acids (Invitrogen), l-glutamine (Invitrogen), and OPI media supplement (Sigma) containing 0.15 mg of oxalacetate, 0.5 g of pyruvate and 8.2 mg of bovine insulin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using the Qiagen RNeasy kit according to the manufacturer's protocol and RNA quality was determined using an Agilent 2100 Bioanalyzer.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | See affymetrix protocol.
| | Sample_hyb_protocol | See affymetrix protocol.
| | Sample_scan_protocol | Affymetrix Gene Chip Scanner 3100.
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Andrew ,P.,Rice
| | Sample_contact_laboratory | Rice
| | Sample_contact_department | Molecular Virology and Microbiology
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257640/suppl/GSM257640.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257640/suppl/GSM257640.CHP.gz
| | Sample_series_id | GSE10234
| | Sample_series_id | GSE11462
| | Sample_data_row_count | 54675
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GSM257656 | GPL570 |
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MM6 cyclin T1 knockdown LPS treated 2
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Mono Mac 6 cells
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Monocytic cell line
|
All the above steps after RNA extraction were done by the Microarray Core Facility at Baylor College of Medicine. This sample is numbered as 9549.
|
| Sample_geo_accession | GSM257656
| | Sample_status | Public on Jul 31 2008
| | Sample_submission_date | Jan 17 2008
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM6 cells were transduced with shRNA against cyclin T1 and then treated with 10 ng/ml LPS.
| | Sample_growth_protocol_ch1 | MM6 cells were maintained in RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Hyclone), ), non-essential amino acids (Invitrogen), l-glutamine (Invitrogen), and OPI media supplement (Sigma) containing 0.15 mg of oxalacetate, 0.5 g of pyruvate and 8.2 mg of bovine insulin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using the Qiagen RNeasy kit according to the manufacturer's protocol and RNA quality was determined using an Agilent 2100 Bioanalyzer.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | See affymetrix protocol.
| | Sample_hyb_protocol | See affymetrix protocol.
| | Sample_scan_protocol | Affymetrix Gene Chip Scanner 3100.
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Andrew ,P.,Rice
| | Sample_contact_laboratory | Rice
| | Sample_contact_department | Molecular Virology and Microbiology
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257656/suppl/GSM257656.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257656/suppl/GSM257656.CHP.gz
| | Sample_series_id | GSE10234
| | Sample_series_id | GSE11462
| | Sample_data_row_count | 54675
| | |
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GSM257657 | GPL570 |
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MM6 control shRNA LPS treated 1
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Mono Mac 6 cells
|
Monocytic cell line
|
All the above steps after RNA extraction were done by the Microarray Core Facility at Baylor College of Medicine. This sample is numbered as 9550.
|
| Sample_geo_accession | GSM257657
| | Sample_status | Public on Jul 31 2008
| | Sample_submission_date | Jan 17 2008
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM6 cells were transduced with a control shRNA and then treated with 10 ng/ml LPS.
| | Sample_growth_protocol_ch1 | MM6 cells were maintained in RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Hyclone), ), non-essential amino acids (Invitrogen), l-glutamine (Invitrogen), and OPI media supplement (Sigma) containing 0.15 mg of oxalacetate, 0.5 g of pyruvate and 8.2 mg of bovine insulin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using the Qiagen RNeasy kit according to the manufacturer's protocol and RNA quality was determined using an Agilent 2100 Bioanalyzer.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | See affymetrix protocol.
| | Sample_hyb_protocol | See affymetrix protocol.
| | Sample_scan_protocol | Affymetrix Gene Chip Scanner 3100.
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Andrew ,P.,Rice
| | Sample_contact_laboratory | Rice
| | Sample_contact_department | Molecular Virology and Microbiology
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257657/suppl/GSM257657.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257657/suppl/GSM257657.CHP.gz
| | Sample_series_id | GSE10234
| | Sample_series_id | GSE11462
| | Sample_data_row_count | 54675
| | |
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GSM257659 | GPL570 |
|
MM6 control shRNA LPS treated 2
|
Mono Mac 6 cells
|
Monocytic cell line
|
All the above steps after RNA extraction were done by the Microarray Core Facility at Baylor College of Medicine. This sample is numbered as 9551.
|
| Sample_geo_accession | GSM257659
| | Sample_status | Public on Jul 31 2008
| | Sample_submission_date | Jan 17 2008
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MM6 cells were transduced with a control shRNA and then treated with 10 ng/ml LPS.
| | Sample_growth_protocol_ch1 | MM6 cells were maintained in RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Hyclone), ), non-essential amino acids (Invitrogen), l-glutamine (Invitrogen), and OPI media supplement (Sigma) containing 0.15 mg of oxalacetate, 0.5 g of pyruvate and 8.2 mg of bovine insulin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using the Qiagen RNeasy kit according to the manufacturer's protocol and RNA quality was determined using an Agilent 2100 Bioanalyzer.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | See affymetrix protocol.
| | Sample_hyb_protocol | See affymetrix protocol.
| | Sample_scan_protocol | Affymetrix Gene Chip Scanner 3100.
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Andrew ,P.,Rice
| | Sample_contact_laboratory | Rice
| | Sample_contact_department | Molecular Virology and Microbiology
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77025
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257659/suppl/GSM257659.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM257nnn/GSM257659/suppl/GSM257659.CHP.gz
| | Sample_series_id | GSE10234
| | Sample_series_id | GSE11462
| | Sample_data_row_count | 54675
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