Result

Search results for the GEO ID: GSE10309

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Title

Source name

Description

Characteristics

GSM260533

GPL570

CL1-5, biological rep1

Human lung adenocarcinoma cell line CL1-5 vector control

Gender: male

The mRNA profiles from vector control transfectants and CLDN1 overexpressed transfectants were quantitatively determined.

Sample_geo_accession	GSM260533
Sample_status	Public on Dec 23 2008
Sample_submission_date	Jan 29 2008
Sample_last_update_date	Dec 23 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Gentamycin (G418) 400 μg/ml
Sample_growth_protocol_ch1	Human lung adenocarcinoma cell lines of CL1-5 CLDN1-transfected and CL1-5 vector control were grown in RPMI 1640 with 10% fetal bovine serum at 37°C, 20% O2, 5% CO2 and 400 μg/ml G418.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
Sample_data_processing	The data were processed by GeneSpring software version 7.2 GC-RMA preprocessor to use on the CEL files
Sample_platform_id	GPL570
Sample_contact_name	Sung-Liang,,Yu
Sample_contact_email	slyu@ntu.edu.tw
Sample_contact_phone	886-2-23958341
Sample_contact_fax	886-2-23958341
Sample_contact_laboratory	Microarray Core Facility
Sample_contact_department	Clinical Laboratory Sciences and Medical Biotechnology
Sample_contact_institute	National Taiwan University
Sample_contact_address	Jen Ai Road Section1
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM260nnn/GSM260533/suppl/GSM260533.CEL.gz
Sample_series_id	GSE10309
Sample_data_row_count	54675

GSM260534

GPL570

CL1-5, biological rep2

Human lung adenocarcinoma cell line CL1-5 vector control

Gender: male

The mRNA profiles from vector control transfectants and CLDN1 overexpressed transfectants were quantitatively determined.

Sample_geo_accession	GSM260534
Sample_status	Public on Dec 23 2008
Sample_submission_date	Jan 29 2008
Sample_last_update_date	Dec 23 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Gentamycin (G418) 400 μg/ml
Sample_growth_protocol_ch1	Human lung adenocarcinoma cell lines of CL1-5 CLDN1-transfected and CL1-5 vector control were grown in RPMI 1640 with 10% fetal bovine serum at 37°C, 20% O2, 5% CO2 and 400 μg/ml G418.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
Sample_data_processing	The data were processed by GeneSpring software version 7.2 GC-RMA preprocessor to use on the CEL files
Sample_platform_id	GPL570
Sample_contact_name	Sung-Liang,,Yu
Sample_contact_email	slyu@ntu.edu.tw
Sample_contact_phone	886-2-23958341
Sample_contact_fax	886-2-23958341
Sample_contact_laboratory	Microarray Core Facility
Sample_contact_department	Clinical Laboratory Sciences and Medical Biotechnology
Sample_contact_institute	National Taiwan University
Sample_contact_address	Jen Ai Road Section1
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM260nnn/GSM260534/suppl/GSM260534.CEL.gz
Sample_series_id	GSE10309
Sample_data_row_count	54675

GSM260535

GPL570

CLDN1 overexpressed CL1-5, biological rep1

Human lung adenocarcinoma cell line CL1-5 CLDN1-transfected

Gender: male

The mRNA profiles from vector control transfectants and CLDN1 overexpressed transfectants were quantitatively determined.

Sample_geo_accession	GSM260535
Sample_status	Public on Dec 23 2008
Sample_submission_date	Jan 29 2008
Sample_last_update_date	Dec 23 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Gentamycin (G418) 400 μg/ml
Sample_growth_protocol_ch1	Human lung adenocarcinoma cell lines of CL1-5 CLDN1-transfected and CL1-5 vector control were grown in RPMI 1640 with 10% fetal bovine serum at 37°C, 20% O2, 5% CO2 and 400 μg/ml G418.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
Sample_data_processing	The data were processed by GeneSpring software version 7.2 GC-RMA preprocessor to use on the CEL files
Sample_platform_id	GPL570
Sample_contact_name	Sung-Liang,,Yu
Sample_contact_email	slyu@ntu.edu.tw
Sample_contact_phone	886-2-23958341
Sample_contact_fax	886-2-23958341
Sample_contact_laboratory	Microarray Core Facility
Sample_contact_department	Clinical Laboratory Sciences and Medical Biotechnology
Sample_contact_institute	National Taiwan University
Sample_contact_address	Jen Ai Road Section1
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM260nnn/GSM260535/suppl/GSM260535.CEL.gz
Sample_series_id	GSE10309
Sample_data_row_count	54675

GSM260536

GPL570

CLDN1 overexpressed CL1-5, biological rep2

Human lung adenocarcinoma cell line CL1-5 CLDN1-transfected

Gender: male

The mRNA profiles from vector control transfectants and CLDN1 overexpressed transfectants were quantitatively determined.

Sample_geo_accession	GSM260536
Sample_status	Public on Dec 23 2008
Sample_submission_date	Jan 29 2008
Sample_last_update_date	Dec 23 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Gentamycin (G418) 400 μg/ml
Sample_growth_protocol_ch1	Human lung adenocarcinoma cell lines of CL1-5 CLDN1-transfected and CL1-5 vector control were grown in RPMI 1640 with 10% fetal bovine serum at 37°C, 20% O2, 5% CO2 and 400 μg/ml G418.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
Sample_data_processing	The data were processed by GeneSpring software version 7.2 GC-RMA preprocessor to use on the CEL files
Sample_platform_id	GPL570
Sample_contact_name	Sung-Liang,,Yu
Sample_contact_email	slyu@ntu.edu.tw
Sample_contact_phone	886-2-23958341
Sample_contact_fax	886-2-23958341
Sample_contact_laboratory	Microarray Core Facility
Sample_contact_department	Clinical Laboratory Sciences and Medical Biotechnology
Sample_contact_institute	National Taiwan University
Sample_contact_address	Jen Ai Road Section1
Sample_contact_city	Taipei
Sample_contact_zip/postal_code	100
Sample_contact_country	Taiwan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM260nnn/GSM260536/suppl/GSM260536.CEL.gz
Sample_series_id	GSE10309
Sample_data_row_count	54675

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