Search results for the GEO ID: GSE10401  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM262846 | GPL339 |
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E2F4WT, chip MOE430A
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Wild type embryonic neurospheres, Forebrain E12.5 (ventral telencephalon)
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Strain: CS7B6
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This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Ruth Slack (rslack@uottawa.ca; ) for analysis.
Stembase Experiment ID: E236
Stembase Experiment ID link: http://www.stembase.ca/?path=/browse/experiment&id=236
SCGP Sample ID: S327
SCGP Sample ID link: http://www.stembase.ca/?path=/browse/experiment&id=236#SAMPLE_365
Short description: E2F4 wild type neurospheres from E12.5 forebrain. E2F4 transcription factor regulates the expression of the genes which are required for neural stem cell expansion in the developing mouse brain. The results of microarray analysis will help to identify the signaling pathways affected by E2F4 deletion and refine the neural stem cell regulatory mechanism.
RNA concentration: 2.0 ug/ul
Sample volume: 10 ul
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| Sample_geo_accession | GSM262846
| | Sample_status | Public on Feb 13 2008
| | Sample_submission_date | Feb 06 2008
| | Sample_last_update_date | Feb 12 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA prep method: RNeasy
| | Sample_extract_protocol_ch1 | Amplification method: none
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from the total RNA according to the Affymetrix protocol (Expression Analysis Technical Manual Rev. 2, 2005-2006, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on the GeneChip Array and the GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to the standard Affymetrix protocol (Expression Analysis Technical Manual Rev. 2, 2005-2006, Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip 3000 7G scanner.
| | Sample_data_processing | Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpha2=0.06
| | Sample_platform_id | GPL339
| | Sample_contact_name | Carol,,Perez-Iratxeta
| | Sample_contact_email | cpereziratxeta@gmail.com
| | Sample_contact_phone | (613) 737-8899 -73255
| | Sample_contact_fax | (613) 737-8023
| | Sample_contact_laboratory | Cellular and Molecular Medicine, Bioinformatics
| | Sample_contact_department | Ottawa Health Research Institute
| | Sample_contact_institute | Ontario Genomics Innovation Centre (OGIC)
| | Sample_contact_address | 501 Smyth Rd.
| | Sample_contact_city | Ottawa
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | K1H 8L6
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://www.ohri.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM262nnn/GSM262846/suppl/GSM262846.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM262nnn/GSM262846/suppl/GSM262846.EXP.gz
| | Sample_series_id | GSE10401
| | Sample_data_row_count | 22690
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GSM262848 | GPL339 |
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E2F4KO, chip MOE430A
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Knockout embryonic neurospheres, Forebrain E12.5 (ventral telencephalon)
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Strain: CS7B6
|
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.stembase.ca). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Ruth Slack (rslack@uottawa.ca; ) for analysis.
Stembase Experiment ID: E236
Stembase Experiment ID link: http://www.stembase.ca/?path=/browse/experiment&id=236
SCGP Sample ID: S328
SCGP Sample ID link: http://www.stembase.ca/?path=/browse/experiment&id=236#SAMPLE_366
Short description: E2F4 knockout neurospheres from E12.5 forebrain. E2F4 transcription factor regulates the expression of the genes which are required for neural stem cell expansion in the developing mouse brain. The results of microarray analysis will help to identify the signaling pathways affected by E2F4 deletion and refine the neural stem cell regulatory mechanism.
RNA concentration: 2.0 ug/ul
Sample volume: 10 ul
|
| Sample_geo_accession | GSM262848
| | Sample_status | Public on Feb 13 2008
| | Sample_submission_date | Feb 06 2008
| | Sample_last_update_date | Feb 12 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA prep method: RNeasy
| | Sample_extract_protocol_ch1 | Amplification method: none
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from the total RNA according to the Affymetrix protocol (Expression Analysis Technical Manual Rev. 2, 2005-2006, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, cRNA was hybridized on the GeneChip Array and the GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to the standard Affymetrix protocol (Expression Analysis Technical Manual Rev. 2, 2005-2006, Affymetrix).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip 3000 7G scanner.
| | Sample_data_processing | Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpha2=0.06
| | Sample_platform_id | GPL339
| | Sample_contact_name | Carol,,Perez-Iratxeta
| | Sample_contact_email | cpereziratxeta@gmail.com
| | Sample_contact_phone | (613) 737-8899 -73255
| | Sample_contact_fax | (613) 737-8023
| | Sample_contact_laboratory | Cellular and Molecular Medicine, Bioinformatics
| | Sample_contact_department | Ottawa Health Research Institute
| | Sample_contact_institute | Ontario Genomics Innovation Centre (OGIC)
| | Sample_contact_address | 501 Smyth Rd.
| | Sample_contact_city | Ottawa
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | K1H 8L6
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://www.ohri.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM262nnn/GSM262848/suppl/GSM262848.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM262nnn/GSM262848/suppl/GSM262848.EXP.gz
| | Sample_series_id | GSE10401
| | Sample_data_row_count | 22690
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