Search results for the GEO ID: GSE10437 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM263933 | GPL570 |
|
CD4+ Tcell Resting M0
|
Human CD4+ T cells, resting
|
Human CD4+ T cells
|
CD4+ Tcell Resting M0
Human CD4+ T cells were prepared from crude lymphocyte fraction by negative selection using CD4+ T cell isolation kit II from Myltenyi Biotech.
|
Sample_geo_accession | GSM263933
| Sample_status | Public on Feb 09 2008
| Sample_submission_date | Feb 07 2008
| Sample_last_update_date | Feb 08 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | None
| Sample_growth_protocol_ch1 | None
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using a Qiagen RNeasy mini kit following the manufacturer's directions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling was performed using Affymetrix's IVT labeling kit following manufacturer's directions.
| Sample_hyb_protocol | 20 micrograms of biotin labeled RNA was fragmented to ~200 bp fragments by incubating in a buffer containing 200 mM Tris-Acetate pH 8.2, 500 mM Potassium Acetate and 500 Magnesium Acetate for 35 minutes at 95C. Fragments were hybridized to Affymetrix U133A plus 2.0 chips for 16 hrs, washed and stained on an Affymetrix fluidics station.
| Sample_scan_protocol | Scanning was performed with the Affymetrix GeneChip scanner using default settings.
| Sample_data_processing | Data processing was performed using the Affymetrix GCOS (v1.40) package with the MAS5 method.
| Sample_platform_id | GPL570
| Sample_contact_name | Dustin,E,Schones
| Sample_contact_email | schonesde@mail.nih.gov
| Sample_contact_phone | 301-402-0970
| Sample_contact_fax | 301-402-0971
| Sample_contact_laboratory | Zhao Lab
| Sample_contact_institute | NHLBI/NIH
| Sample_contact_address | Building 10, Room 7B20A, 9000 Rockville Pike
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM263nnn/GSM263933/suppl/GSM263933.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM263nnn/GSM263933/suppl/GSM263933.CHP.gz
| Sample_series_id | GSE10437
| Sample_data_row_count | 54675
| |
|
GSM263934 | GPL570 |
|
CD4+ Tcell Resting T0
|
Human CD4+ T cells, resting
|
Human CD4+ T cells
|
CD4+ Tcell Resting T0
Human CD4+ T cells were prepared from crude lymphocyte fraction by negative selection using CD4+ T cell isolation kit II from Myltenyi Biotech.
|
Sample_geo_accession | GSM263934
| Sample_status | Public on Feb 09 2008
| Sample_submission_date | Feb 07 2008
| Sample_last_update_date | Feb 08 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | None
| Sample_growth_protocol_ch1 | None
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using a Qiagen RNeasy mini kit following the manufacturer's directions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling was performed using Affymetrix's IVT labeling kit following manufacturer's directions.
| Sample_hyb_protocol | 20 micrograms of biotin labeled RNA was fragmented to ~200 bp fragments by incubating in a buffer containing 200 mM Tris-Acetate pH 8.2, 500 mM Potassium Acetate and 500 Magnesium Acetate for 35 minutes at 95C. Fragments were hybridized to Affymetrix U133A plus 2.0 chips for 16 hrs, washed and stained on an Affymetrix fluidics station.
| Sample_scan_protocol | Scanning was performed with the Affymetrix GeneChip scanner using default settings.
| Sample_data_processing | Data processing was performed using the Affymetrix GCOS (v1.40) package with the MAS5 method.
| Sample_platform_id | GPL570
| Sample_contact_name | Dustin,E,Schones
| Sample_contact_email | schonesde@mail.nih.gov
| Sample_contact_phone | 301-402-0970
| Sample_contact_fax | 301-402-0971
| Sample_contact_laboratory | Zhao Lab
| Sample_contact_institute | NHLBI/NIH
| Sample_contact_address | Building 10, Room 7B20A, 9000 Rockville Pike
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM263nnn/GSM263934/suppl/GSM263934.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM263nnn/GSM263934/suppl/GSM263934.CHP.gz
| Sample_series_id | GSE10437
| Sample_data_row_count | 54675
| |
|
GSM263935 | GPL570 |
|
CD4+ Tcell Activated M18
|
Human CD4+ T cells, activated using anti-CD3/28 beads (Invitrogen), 18hrs
|
Human CD4+ T cells
|
CD4+ Tcell Activated M18
Human CD4+ T cells were prepared from crude lymphocyte fraction by negative selection using CD4+ T cell isolation kit II from Myltenyi Biotech.
|
Sample_geo_accession | GSM263935
| Sample_status | Public on Feb 09 2008
| Sample_submission_date | Feb 07 2008
| Sample_last_update_date | Feb 08 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | anti-CD3/28 beads (Invitrogen), 1 bead per cell, 18hrs
| Sample_growth_protocol_ch1 | RPMI-1640, 10% FCS, antibiotics
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using a Qiagen RNeasy mini kit following the manufacturer's directions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling was performed using Affymetrix's IVT labeling kit following manufacturer's directions.
| Sample_hyb_protocol | 20 micrograms of biotin labeled RNA was fragmented to ~200 bp fragments by incubating in a buffer containing 200 mM Tris-Acetate pH 8.2, 500 mM Potassium Acetate and 500 Magnesium Acetate for 35 minutes at 95C. Fragments were hybridized to Affymetrix U133A plus 2.0 chips for 16 hrs, washed and stained on an Affymetrix fluidics station.
| Sample_scan_protocol | Scanning was performed with the Affymetrix GeneChip scanner using default settings.
| Sample_data_processing | Data processing was performed using the Affymetrix GCOS (v1.40) package with the MAS5 method.
| Sample_platform_id | GPL570
| Sample_contact_name | Dustin,E,Schones
| Sample_contact_email | schonesde@mail.nih.gov
| Sample_contact_phone | 301-402-0970
| Sample_contact_fax | 301-402-0971
| Sample_contact_laboratory | Zhao Lab
| Sample_contact_institute | NHLBI/NIH
| Sample_contact_address | Building 10, Room 7B20A, 9000 Rockville Pike
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM263nnn/GSM263935/suppl/GSM263935.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM263nnn/GSM263935/suppl/GSM263935.CHP.gz
| Sample_series_id | GSE10437
| Sample_data_row_count | 54675
| |
|
GSM263936 | GPL570 |
|
CD4+ Tcell Activated T18
|
Human CD4+ T cells, activated using anti-CD3/28 beads (Invitrogen), 18hrs
|
Human CD4+ T cells
|
CD4+ Tcell Activated T18
Human CD4+ T cells were prepared from crude lymphocyte fraction by negative selection using CD4+ T cell isolation kit II from Myltenyi Biotech.
|
Sample_geo_accession | GSM263936
| Sample_status | Public on Feb 09 2008
| Sample_submission_date | Feb 07 2008
| Sample_last_update_date | Feb 08 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | anti-CD3/28 beads (Invitrogen), 1 bead per cell, 18hrs
| Sample_growth_protocol_ch1 | RPMI-1640, 10% FCS, antibiotics
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extraction was performed using a Qiagen RNeasy mini kit following the manufacturer's directions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeling was performed using Affymetrix's IVT labeling kit following manufacturer's directions.
| Sample_hyb_protocol | 20 micrograms of biotin labeled RNA was fragmented to ~200 bp fragments by incubating in a buffer containing 200 mM Tris-Acetate pH 8.2, 500 mM Potassium Acetate and 500 Magnesium Acetate for 35 minutes at 95C. Fragments were hybridized to Affymetrix U133A plus 2.0 chips for 16 hrs, washed and stained on an Affymetrix fluidics station.
| Sample_scan_protocol | Scanning was performed with the Affymetrix GeneChip scanner using default settings.
| Sample_data_processing | Data processing was performed using the Affymetrix GCOS (v1.40) package with the MAS5 method.
| Sample_platform_id | GPL570
| Sample_contact_name | Dustin,E,Schones
| Sample_contact_email | schonesde@mail.nih.gov
| Sample_contact_phone | 301-402-0970
| Sample_contact_fax | 301-402-0971
| Sample_contact_laboratory | Zhao Lab
| Sample_contact_institute | NHLBI/NIH
| Sample_contact_address | Building 10, Room 7B20A, 9000 Rockville Pike
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM263nnn/GSM263936/suppl/GSM263936.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM263nnn/GSM263936/suppl/GSM263936.CHP.gz
| Sample_series_id | GSE10437
| Sample_data_row_count | 54675
| |
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