Search results for the GEO ID: GSE10463 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM264755 | GPL570 |
|
hMoDC_stim_8h_ctrl_rep1
|
immature human monocyte-derived dendritic cells (MoDCs)
|
8h, stimulated, not treated
Donor D1_ctrl
|
na
|
Sample_geo_accession | GSM264755
| Sample_status | Public on Feb 12 2008
| Sample_submission_date | Feb 11 2008
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Immature monocyte-derived DC were activated with anti-CD40 antibodies for 8 hours in the absence or presence of 50nM VAF347.
| Sample_growth_protocol_ch1 | Human peripheral blood monocytes were prepared by elutriation or by negative selection of PBMC using a monocyte isolation kit. Monocytes (typically >97% positive for CD14) were differentiated into immature DC by adding 40ng/ml IL-4 and 15ng/ml or 50ng/ml GM-CSF for 6-8 days in the absence or presence of increasing concentrations of VAF347.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Absolutely RNA kit (Stratagene)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Double-stranded cDNA synthesis was carried out with total RNA (SuperScript double-stranded cDNA synthesis kit; Invitrogen) according to the manufacturer’s instructions.
| Sample_label_protocol_ch1 | After purification (QIAquick; Qiagen), the cDNA was transcribed in vitro in the presence of biotinylated ribonucleotides (Bioarray High yield T7 DNA transcription kit, ENZO Life Sciences Inc.). The labeled cRNA was purified on an affinity resin (RNeasy, Qiagen), quantified and fragmented into strands of approximately 50-200 nucleotides in length.
| Sample_hyb_protocol | Hybridization was carried out at 45ºC for approximately 18 hr.
| Sample_scan_protocol | The arrays were stained on a GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| Sample_data_processing | The obtained signal intensities (.CEL files) were uploaded into GeneSpring (Agilent Technologies)
| Sample_platform_id | GPL570
| Sample_contact_name | Markus,,Jaritz
| Sample_contact_email | markus.jaritz@imp.ac.at
| Sample_contact_institute | IMP
| Sample_contact_address | Dr. Bohr-Gasse 7
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | A-1030
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM264nnn/GSM264755/suppl/GSM264755.cel.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE10463
| Sample_data_row_count | 54675
| |
|
GSM264756 | GPL570 |
|
hMoDC_stim_8h_ctrl_rep2
|
immature human monocyte-derived dendritic cells (MoDCs)
|
8h, stimulated, not treated
Donor D2_ctrl
|
na
|
Sample_geo_accession | GSM264756
| Sample_status | Public on Feb 12 2008
| Sample_submission_date | Feb 11 2008
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Immature monocyte-derived DC were activated with anti-CD40 antibodies for 8 hours in the absence or presence of 50nM VAF347.
| Sample_growth_protocol_ch1 | Human peripheral blood monocytes were prepared by elutriation or by negative selection of PBMC using a monocyte isolation kit. Monocytes (typically >97% positive for CD14) were differentiated into immature DC by adding 40ng/ml IL-4 and 15ng/ml or 50ng/ml GM-CSF for 6-8 days in the absence or presence of increasing concentrations of VAF347.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Absolutely RNA kit (Stratagene)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Double-stranded cDNA synthesis was carried out with total RNA (SuperScript double-stranded cDNA synthesis kit; Invitrogen) according to the manufacturer’s instructions.
| Sample_label_protocol_ch1 | After purification (QIAquick; Qiagen), the cDNA was transcribed in vitro in the presence of biotinylated ribonucleotides (Bioarray High yield T7 DNA transcription kit, ENZO Life Sciences Inc.). The labeled cRNA was purified on an affinity resin (RNeasy, Qiagen), quantified and fragmented into strands of approximately 50-200 nucleotides in length.
| Sample_hyb_protocol | Hybridization was carried out at 45ºC for approximately 18 hr.
| Sample_scan_protocol | The arrays were stained on a GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| Sample_data_processing | The obtained signal intensities (.CEL files) were uploaded into GeneSpring (Agilent Technologies)
| Sample_platform_id | GPL570
| Sample_contact_name | Markus,,Jaritz
| Sample_contact_email | markus.jaritz@imp.ac.at
| Sample_contact_institute | IMP
| Sample_contact_address | Dr. Bohr-Gasse 7
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | A-1030
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM264nnn/GSM264756/suppl/GSM264756.cel.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE10463
| Sample_data_row_count | 54675
| |
|
GSM264757 | GPL570 |
|
hMoDC_stim_8h_VAF347_rep1
|
immature human monocyte-derived dendritic cells (MoDCs)
|
8h, stimulated, treated with VAF347
Donor D1_VAF347
|
na
|
Sample_geo_accession | GSM264757
| Sample_status | Public on Feb 12 2008
| Sample_submission_date | Feb 11 2008
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Immature monocyte-derived DC were activated with anti-CD40 antibodies for 8 hours in the absence or presence of 50nM VAF347.
| Sample_growth_protocol_ch1 | Human peripheral blood monocytes were prepared by elutriation or by negative selection of PBMC using a monocyte isolation kit. Monocytes (typically >97% positive for CD14) were differentiated into immature DC by adding 40ng/ml IL-4 and 15ng/ml or 50ng/ml GM-CSF for 6-8 days in the absence or presence of increasing concentrations of VAF347.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Absolutely RNA kit (Stratagene)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Double-stranded cDNA synthesis was carried out with total RNA (SuperScript double-stranded cDNA synthesis kit; Invitrogen) according to the manufacturer’s instructions.
| Sample_label_protocol_ch1 | After purification (QIAquick; Qiagen), the cDNA was transcribed in vitro in the presence of biotinylated ribonucleotides (Bioarray High yield T7 DNA transcription kit, ENZO Life Sciences Inc.). The labeled cRNA was purified on an affinity resin (RNeasy, Qiagen), quantified and fragmented into strands of approximately 50-200 nucleotides in length.
| Sample_hyb_protocol | Hybridization was carried out at 45ºC for approximately 18 hr.
| Sample_scan_protocol | The arrays were stained on a GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| Sample_data_processing | The obtained signal intensities (.CEL files) were uploaded into GeneSpring (Agilent Technologies)
| Sample_platform_id | GPL570
| Sample_contact_name | Markus,,Jaritz
| Sample_contact_email | markus.jaritz@imp.ac.at
| Sample_contact_institute | IMP
| Sample_contact_address | Dr. Bohr-Gasse 7
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | A-1030
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM264nnn/GSM264757/suppl/GSM264757.cel.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE10463
| Sample_data_row_count | 54675
| |
|
GSM264758 | GPL570 |
|
hMoDC_stim_8h_VAF347_rep2
|
immature human monocyte-derived dendritic cells (MoDCs)
|
8h, stimulated, treated with VAF347
Donor D2_VAF347
|
na
|
Sample_geo_accession | GSM264758
| Sample_status | Public on Feb 12 2008
| Sample_submission_date | Feb 11 2008
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Immature monocyte-derived DC were activated with anti-CD40 antibodies for 8 hours in the absence or presence of 50nM VAF347.
| Sample_growth_protocol_ch1 | Human peripheral blood monocytes were prepared by elutriation or by negative selection of PBMC using a monocyte isolation kit. Monocytes (typically >97% positive for CD14) were differentiated into immature DC by adding 40ng/ml IL-4 and 15ng/ml or 50ng/ml GM-CSF for 6-8 days in the absence or presence of increasing concentrations of VAF347.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Absolutely RNA kit (Stratagene)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Double-stranded cDNA synthesis was carried out with total RNA (SuperScript double-stranded cDNA synthesis kit; Invitrogen) according to the manufacturer’s instructions.
| Sample_label_protocol_ch1 | After purification (QIAquick; Qiagen), the cDNA was transcribed in vitro in the presence of biotinylated ribonucleotides (Bioarray High yield T7 DNA transcription kit, ENZO Life Sciences Inc.). The labeled cRNA was purified on an affinity resin (RNeasy, Qiagen), quantified and fragmented into strands of approximately 50-200 nucleotides in length.
| Sample_hyb_protocol | Hybridization was carried out at 45ºC for approximately 18 hr.
| Sample_scan_protocol | The arrays were stained on a GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
| Sample_data_processing | The obtained signal intensities (.CEL files) were uploaded into GeneSpring (Agilent Technologies)
| Sample_platform_id | GPL570
| Sample_contact_name | Markus,,Jaritz
| Sample_contact_email | markus.jaritz@imp.ac.at
| Sample_contact_institute | IMP
| Sample_contact_address | Dr. Bohr-Gasse 7
| Sample_contact_city | Vienna
| Sample_contact_zip/postal_code | A-1030
| Sample_contact_country | Austria
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM264nnn/GSM264758/suppl/GSM264758.cel.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE10463
| Sample_data_row_count | 54675
| |
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