Search results for the GEO ID: GSE10479 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM265052 | GPL570 |
|
bg-CAT treated HUVECs, biological rep1
|
primary cultured HUVECs treated with bg-CAT (25 nM, 2 h)
|
Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
The primary cultured HUVECs were confirmed by positive staining of indirect immunofluorescence with thrombomodulin (monoclonal antibody).
|
Gene expression data from HUVECs treated with bg-CAT (25 nM) for 2 hour
|
Sample_geo_accession | GSM265052
| Sample_status | Public on Apr 30 2008
| Sample_submission_date | Feb 12 2008
| Sample_last_update_date | Feb 12 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary cultured HUVECs treated with bg-CAT (25 nM) at 37 degree for 2 hour and PBS-treated cells as a normal control.
| Sample_growth_protocol_ch1 | Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on human genome-U133 plus 2.0 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS 1.4),and using Affymetrix default analysis settings and global scaling as normalization method (Microarray Suite version 5.0, MAS5.0). The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | bai,shu,liu
| Sample_contact_email | liutonny@hotmail.com
| Sample_contact_phone | 86-871-5194279
| Sample_contact_fax | 86-871-5198515
| Sample_contact_institute | kunming institute of zoology
| Sample_contact_address | jiaochang east road 32#
| Sample_contact_city | kunming
| Sample_contact_state | yunnan
| Sample_contact_zip/postal_code | 650223
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265052/suppl/GSM265052.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265052/suppl/GSM265052.EXP.gz
| Sample_series_id | GSE10479
| Sample_data_row_count | 54675
| |
|
GSM265053 | GPL570 |
|
bg-CAT treated HUVECs, biological rep2
|
primary cultured HUVECs treated with bg-CAT (25 nM, 2 h)
|
Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
The primary cultured HUVECs were confirmed by positive staining of indirect immunofluorescence with thrombomodulin (monoclonal antibody).
|
Gene expression data from HUVECs treated with bg-CAT (25 nM) for 2 hour
|
Sample_geo_accession | GSM265053
| Sample_status | Public on Apr 30 2008
| Sample_submission_date | Feb 12 2008
| Sample_last_update_date | Feb 12 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary cultured HUVECs treated with bg-CAT (25 nM) at 37 degree for 2 hour and PBS-treated cells as a normal control.
| Sample_growth_protocol_ch1 | Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on human genome-U133 plus 2.0 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS 1.4),and using Affymetrix default analysis settings and global scaling as normalization method (Microarray Suite version 5.0, MAS5.0). The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | bai,shu,liu
| Sample_contact_email | liutonny@hotmail.com
| Sample_contact_phone | 86-871-5194279
| Sample_contact_fax | 86-871-5198515
| Sample_contact_institute | kunming institute of zoology
| Sample_contact_address | jiaochang east road 32#
| Sample_contact_city | kunming
| Sample_contact_state | yunnan
| Sample_contact_zip/postal_code | 650223
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265053/suppl/GSM265053.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265053/suppl/GSM265053.EXP.gz
| Sample_series_id | GSE10479
| Sample_data_row_count | 54675
| |
|
GSM265054 | GPL570 |
|
bg-CAT treated HUVECs, biological rep3
|
primary cultured HUVECs treated with bg-CAT (25 nM, 2 h)
|
Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
The primary cultured HUVECs were confirmed by positive staining of indirect immunofluorescence with thrombomodulin (monoclonal antibody).
|
Gene expression data from HUVECs treated with bg-CAT (25 nM) for 2 hour
|
Sample_geo_accession | GSM265054
| Sample_status | Public on Apr 30 2008
| Sample_submission_date | Feb 12 2008
| Sample_last_update_date | Feb 12 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary cultured HUVECs treated with bg-CAT (25 nM) at 37 degree for 2 hour and PBS-treated cells as a normal control.
| Sample_growth_protocol_ch1 | Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on human genome-U133 plus 2.0 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS 1.4),and using Affymetrix default analysis settings and global scaling as normalization method (Microarray Suite version 5.0, MAS5.0). The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | bai,shu,liu
| Sample_contact_email | liutonny@hotmail.com
| Sample_contact_phone | 86-871-5194279
| Sample_contact_fax | 86-871-5198515
| Sample_contact_institute | kunming institute of zoology
| Sample_contact_address | jiaochang east road 32#
| Sample_contact_city | kunming
| Sample_contact_state | yunnan
| Sample_contact_zip/postal_code | 650223
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265054/suppl/GSM265054.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265054/suppl/GSM265054.EXP.gz
| Sample_series_id | GSE10479
| Sample_data_row_count | 54675
| |
|
GSM265055 | GPL570 |
|
bg-CAT treated HUVECs, biological rep4
|
primary cultured HUVECs treated with bg-CAT (25 nM, 2 h)
|
Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
The primary cultured HUVECs were confirmed by positive staining of indirect immunofluorescence with thrombomodulin (monoclonal antibody).
|
Gene expression data from HUVECs treated with bg-CAT (25 nM) for 2 hour
|
Sample_geo_accession | GSM265055
| Sample_status | Public on Apr 30 2008
| Sample_submission_date | Feb 12 2008
| Sample_last_update_date | Feb 12 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary cultured HUVECs treated with bg-CAT (25 nM) at 37 degree for 2 hour and PBS-treated cells as a normal control.
| Sample_growth_protocol_ch1 | Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on human genome-U133 plus 2.0 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS 1.4),and using Affymetrix default analysis settings and global scaling as normalization method (Microarray Suite version 5.0, MAS5.0). The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | bai,shu,liu
| Sample_contact_email | liutonny@hotmail.com
| Sample_contact_phone | 86-871-5194279
| Sample_contact_fax | 86-871-5198515
| Sample_contact_institute | kunming institute of zoology
| Sample_contact_address | jiaochang east road 32#
| Sample_contact_city | kunming
| Sample_contact_state | yunnan
| Sample_contact_zip/postal_code | 650223
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265055/suppl/GSM265055.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265055/suppl/GSM265055.EXP.gz
| Sample_series_id | GSE10479
| Sample_data_row_count | 54675
| |
|
GSM265056 | GPL570 |
|
HUVECs normal Control, biological rep1
|
primary cultured HUVECs treated with PBS as a normal control
|
Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
The primary cultured HUVECs were confirmed by positive staining of indirect immunofluorescence with thrombomodulin (monoclonal antibody).
|
Gene expression data from normal control HUVECs
|
Sample_geo_accession | GSM265056
| Sample_status | Public on Apr 30 2008
| Sample_submission_date | Feb 12 2008
| Sample_last_update_date | Feb 12 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary cultured HUVECs treated with bg-CAT (25 nM) at 37 degree for 2 hour and PBS-treated cells as a normal control.
| Sample_growth_protocol_ch1 | Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on human genome-U133 plus 2.0 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS 1.4),and using Affymetrix default analysis settings and global scaling as normalization method (Microarray Suite version 5.0, MAS5.0). The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | bai,shu,liu
| Sample_contact_email | liutonny@hotmail.com
| Sample_contact_phone | 86-871-5194279
| Sample_contact_fax | 86-871-5198515
| Sample_contact_institute | kunming institute of zoology
| Sample_contact_address | jiaochang east road 32#
| Sample_contact_city | kunming
| Sample_contact_state | yunnan
| Sample_contact_zip/postal_code | 650223
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265056/suppl/GSM265056.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265056/suppl/GSM265056.EXP.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_series_id | GSE10479
| Sample_data_row_count | 54675
| |
|
GSM265057 | GPL570 |
|
HUVECs normal Control, biological rep2
|
primary cultured HUVECs treated with PBS as a normal control
|
Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
The primary cultured HUVECs were confirmed by positive staining of indirect immunofluorescence with thrombomodulin (monoclonal antibody).
|
Gene expression data from normal control HUVECs
|
Sample_geo_accession | GSM265057
| Sample_status | Public on Apr 30 2008
| Sample_submission_date | Feb 12 2008
| Sample_last_update_date | Feb 12 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary cultured HUVECs treated with bg-CAT (25 nM) at 37 degree for 2 hour and PBS-treated cells as a normal control.
| Sample_growth_protocol_ch1 | Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on human genome-U133 plus 2.0 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS 1.4),and using Affymetrix default analysis settings and global scaling as normalization method (Microarray Suite version 5.0, MAS5.0). The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | bai,shu,liu
| Sample_contact_email | liutonny@hotmail.com
| Sample_contact_phone | 86-871-5194279
| Sample_contact_fax | 86-871-5198515
| Sample_contact_institute | kunming institute of zoology
| Sample_contact_address | jiaochang east road 32#
| Sample_contact_city | kunming
| Sample_contact_state | yunnan
| Sample_contact_zip/postal_code | 650223
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265057/suppl/GSM265057.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265057/suppl/GSM265057.EXP.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_series_id | GSE10479
| Sample_data_row_count | 54675
| |
|
GSM265058 | GPL570 |
|
HUVECs normal Control, biological rep3
|
primary cultured HUVECs treated with PBS as a normal control
|
Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
The primary cultured HUVECs were confirmed by positive staining of indirect immunofluorescence with thrombomodulin (monoclonal antibody).
|
Gene expression data from normal control HUVECs
|
Sample_geo_accession | GSM265058
| Sample_status | Public on Apr 30 2008
| Sample_submission_date | Feb 12 2008
| Sample_last_update_date | Feb 12 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary cultured HUVECs treated with bg-CAT (25 nM) at 37 degree for 2 hour and PBS-treated cells as a normal control.
| Sample_growth_protocol_ch1 | Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on human genome-U133 plus 2.0 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS 1.4),and using Affymetrix default analysis settings and global scaling as normalization method (Microarray Suite version 5.0, MAS5.0). The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | bai,shu,liu
| Sample_contact_email | liutonny@hotmail.com
| Sample_contact_phone | 86-871-5194279
| Sample_contact_fax | 86-871-5198515
| Sample_contact_institute | kunming institute of zoology
| Sample_contact_address | jiaochang east road 32#
| Sample_contact_city | kunming
| Sample_contact_state | yunnan
| Sample_contact_zip/postal_code | 650223
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265058/suppl/GSM265058.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265058/suppl/GSM265058.EXP.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_series_id | GSE10479
| Sample_data_row_count | 54675
| |
|
GSM265059 | GPL570 |
|
HUVECs normal Control, biological rep4
|
primary cultured HUVECs treated with PBS as a normal control
|
Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
The primary cultured HUVECs were confirmed by positive staining of indirect immunofluorescence with thrombomodulin (monoclonal antibody).
|
Gene expression data from normal control HUVECs
|
Sample_geo_accession | GSM265059
| Sample_status | Public on Apr 30 2008
| Sample_submission_date | Feb 12 2008
| Sample_last_update_date | Feb 12 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary cultured HUVECs treated with bg-CAT (25 nM) at 37 degree for 2 hour and PBS-treated cells as a normal control.
| Sample_growth_protocol_ch1 | Primary cultured HUVECs used in the experiments were confluent monolayers between passages 2 and 3.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on human genome-U133 plus 2.0 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS 1.4),and using Affymetrix default analysis settings and global scaling as normalization method (Microarray Suite version 5.0, MAS5.0). The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | bai,shu,liu
| Sample_contact_email | liutonny@hotmail.com
| Sample_contact_phone | 86-871-5194279
| Sample_contact_fax | 86-871-5198515
| Sample_contact_institute | kunming institute of zoology
| Sample_contact_address | jiaochang east road 32#
| Sample_contact_city | kunming
| Sample_contact_state | yunnan
| Sample_contact_zip/postal_code | 650223
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265059/suppl/GSM265059.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265059/suppl/GSM265059.EXP.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_series_id | GSE10479
| Sample_data_row_count | 54675
| |
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