Result

Search results for the GEO ID: GSE10520

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM265817

GPL570

U937 cells expressing AML1/ETO - Replica 1

U937 myelomoncytic cell line, transfected with HA-tagged AML1/ETO

U937 cell line (human cell line established from a diffuse histiocytic lymphoma and displaying monocytic characteristics) transfected with HA-tagged AML1/ETO under the control of the mouse metallothionine promoter treated for 8h with 100µM ZnSO4 to induce transgene expression.

U937 cells expressing AML1/ETO - Replica 1

Sample_geo_accession	GSM265817
Sample_status	Public on Nov 28 2008
Sample_submission_date	Feb 14 2008
Sample_last_update_date	Nov 05 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cell lines were treated for 8h with 100uM ZnSO4 prior to RNA extraction.
Sample_growth_protocol_ch1	U937 cell lines were maintained in RPMI-1640 supplemented with 10% FCS and 2mM glutamine at 37°C in a humidified atmosphere containing 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol Reagent (Gibco), followed by clean up on RNeasy mini/midi columns (RNeasy Mini/Midi Kit, Qiagen). For each cell line, an RNA pool was obtained by mixing equal quantities of total RNA from each of the three independent RNA extractions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA The size and the accuracy of quantitation of targets were checked by agarose gel electrophoresis of 2ug aliquots, prior to and after fragmentation. After fragmentation, targets were diluted in hybridisation buffer at a concentration of 150ug/ml.
Sample_hyb_protocol	Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip GeneChip HG-U133 Plus v.2 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station. Two copies of the GeneChip HG-U133 Plus v.2 were hybridized with each biotin-labeled target.
Sample_scan_protocol	Images were scanned using an Affymetrix GeneArray Scanner, using default parameters.
Sample_data_processing	The data were analyzed with GeneChip operating system (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Myriam,,Alcalay
Sample_contact_email	myriam.alcalay@ifom-ieo-campus.it
Sample_contact_laboratory	Functional Genomics
Sample_contact_department	Experimental Oncology
Sample_contact_institute	European Institute of Oncology
Sample_contact_address	Via Adamello 16
Sample_contact_city	Milan
Sample_contact_zip/postal_code	20139
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265817/suppl/GSM265817.CEL.gz
Sample_series_id	GSE10520
Sample_series_id	GSE10537
Sample_data_row_count	54675

GSM265818

GPL570

U937 cells expressing AML1/ETO - Replica 2

U937 myelomoncytic cell line, transfected with HA-tagged AML1/ETO

U937 cells expressing AML1/ETO - Replica 2

Sample_geo_accession	GSM265818
Sample_status	Public on Nov 28 2008
Sample_submission_date	Feb 14 2008
Sample_last_update_date	Nov 05 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cell lines were treated for 8h with 100uM ZnSO4 prior to RNA extraction.
Sample_growth_protocol_ch1	U937 cell lines were maintained in RPMI-1640 supplemented with 10% FCS and 2mM glutamine at 37°C in a humidified atmosphere containing 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol Reagent (Gibco), followed by clean up on RNeasy mini/midi columns (RNeasy Mini/Midi Kit, Qiagen). For each cell line, an RNA pool was obtained by mixing equal quantities of total RNA from each of the three independent RNA extractions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA The size and the accuracy of quantitation of targets were checked by agarose gel electrophoresis of 2ug aliquots, prior to and after fragmentation. After fragmentation, targets were diluted in hybridisation buffer at a concentration of 150ug/ml.
Sample_hyb_protocol	Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip GeneChip HG-U133 Plus v.2 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station. Two copies of the GeneChip HG-U133 Plus v.2 were hybridized with each biotin-labeled target.
Sample_scan_protocol	Images were scanned using an Affymetrix GeneArray Scanner, using default parameters.
Sample_data_processing	The data were analyzed with GeneChip operating system (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Myriam,,Alcalay
Sample_contact_email	myriam.alcalay@ifom-ieo-campus.it
Sample_contact_laboratory	Functional Genomics
Sample_contact_department	Experimental Oncology
Sample_contact_institute	European Institute of Oncology
Sample_contact_address	Via Adamello 16
Sample_contact_city	Milan
Sample_contact_zip/postal_code	20139
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265818/suppl/GSM265818.CEL.gz
Sample_series_id	GSE10520
Sample_series_id	GSE10537
Sample_data_row_count	54675

GSM265819

GPL570

U937 cells transfected with empty pSGMtNeo vector - Replica 1

U937 myelomoncytic cell line, transfected with pSGMtNeo vector

U937 cell line (human cell line established from a diffuse histiocytic lymphoma and displaying monocytic characteristics) transfected with pSGMtNeo vector treated for 8h with 100µM ZnSO4.

U937 cells transfected with empty pSGMtNeo vector - Replica 1

Sample_geo_accession	GSM265819
Sample_status	Public on Nov 28 2008
Sample_submission_date	Feb 14 2008
Sample_last_update_date	Nov 05 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cell lines were treated for 8h with 100uM ZnSO4 prior to RNA extraction.
Sample_growth_protocol_ch1	U937 cell lines were maintained in RPMI-1640 supplemented with 10% FCS and 2mM glutamine at 37°C in a humidified atmosphere containing 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol Reagent (Gibco), followed by clean up on RNeasy mini/midi columns (RNeasy Mini/Midi Kit, Qiagen). For each cell line, an RNA pool was obtained by mixing equal quantities of total RNA from each of the three independent RNA extractions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA The size and the accuracy of quantitation of targets were checked by agarose gel electrophoresis of 2ug aliquots, prior to and after fragmentation. After fragmentation, targets were diluted in hybridisation buffer at a concentration of 150ug/ml.
Sample_hyb_protocol	Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip GeneChip HG-U133 Plus v.2 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station. Two copies of the GeneChip HG-U133 Plus v.2 were hybridized with each biotin-labeled target.
Sample_scan_protocol	Images were scanned using an Affymetrix GeneArray Scanner, using default parameters.
Sample_data_processing	The data were analyzed with GeneChip operating system (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Myriam,,Alcalay
Sample_contact_email	myriam.alcalay@ifom-ieo-campus.it
Sample_contact_laboratory	Functional Genomics
Sample_contact_department	Experimental Oncology
Sample_contact_institute	European Institute of Oncology
Sample_contact_address	Via Adamello 16
Sample_contact_city	Milan
Sample_contact_zip/postal_code	20139
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265819/suppl/GSM265819.CEL.gz
Sample_series_id	GSE10520
Sample_series_id	GSE10537
Sample_data_row_count	54675

GSM265820

GPL570

U937 cells transfected with empty pSGMtNeo vector - Replica 2

U937 myelomoncytic cell line, transfected with pSGMtNeo vector

U937 cell line (human cell line established from a diffuse histiocytic lymphoma and displaying monocytic characteristics) transfected with pSGMtNeo vector treated for 8h with 100µM ZnSO4.

U937 cells transfected with empty pSGMtNeo vector - Replica 2

Sample_geo_accession	GSM265820
Sample_status	Public on Nov 28 2008
Sample_submission_date	Feb 14 2008
Sample_last_update_date	Nov 05 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cell lines were treated for 8h with 100uM ZnSO4 prior to RNA extraction.
Sample_growth_protocol_ch1	U937 cell lines were maintained in RPMI-1640 supplemented with 10% FCS and 2mM glutamine at 37°C in a humidified atmosphere containing 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol Reagent (Gibco), followed by clean up on RNeasy mini/midi columns (RNeasy Mini/Midi Kit, Qiagen). For each cell line, an RNA pool was obtained by mixing equal quantities of total RNA from each of the three independent RNA extractions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA The size and the accuracy of quantitation of targets were checked by agarose gel electrophoresis of 2ug aliquots, prior to and after fragmentation. After fragmentation, targets were diluted in hybridisation buffer at a concentration of 150ug/ml.
Sample_hyb_protocol	Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip GeneChip HG-U133 Plus v.2 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station. Two copies of the GeneChip HG-U133 Plus v.2 were hybridized with each biotin-labeled target.
Sample_scan_protocol	Images were scanned using an Affymetrix GeneArray Scanner, using default parameters.
Sample_data_processing	The data were analyzed with GeneChip operating system (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Myriam,,Alcalay
Sample_contact_email	myriam.alcalay@ifom-ieo-campus.it
Sample_contact_laboratory	Functional Genomics
Sample_contact_department	Experimental Oncology
Sample_contact_institute	European Institute of Oncology
Sample_contact_address	Via Adamello 16
Sample_contact_city	Milan
Sample_contact_zip/postal_code	20139
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM265nnn/GSM265820/suppl/GSM265820.CEL.gz
Sample_series_id	GSE10520
Sample_series_id	GSE10537
Sample_data_row_count	54675

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