Search results for the GEO ID: GSE10533  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM266034 | GPL339 |
|
Muscle control 19
|
calf muscle, no treatment, 12 days
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
normally housed three per cage
|
| Sample_geo_accession | GSM266034
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266034/suppl/GSM266034.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266035 | GPL339 |
|
Muscle control 1
|
calf muscle, no treatment, 12 days
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
normally housed three per cage
|
| Sample_geo_accession | GSM266035
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266035/suppl/GSM266035.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266036 | GPL339 |
|
Muscle control 21
|
calf muscle, no treatment, 12 days
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
normally housed three per cage
|
| Sample_geo_accession | GSM266036
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266036/suppl/GSM266036.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266037 | GPL339 |
|
Muscle control 22
|
calf muscle, no treatment, 12 days
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
normally housed three per cage
|
| Sample_geo_accession | GSM266037
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266037/suppl/GSM266037.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266038 | GPL339 |
|
Muscle control 2
|
calf muscle, no treatment, 12 days
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
normally housed three per cage
|
| Sample_geo_accession | GSM266038
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266038/suppl/GSM266038.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266039 | GPL339 |
|
Muscle reloaded 14
|
calf muscle, hindlimb suspension, 12 days plus reloading for 3.5hr
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
mice were suspended at approximately a 30° angle by taping the tail to a plastic dowel attached to a swivel apparatus; at 12 days mice were unsuspended for 3.5 hours
|
| Sample_geo_accession | GSM266039
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266039/suppl/GSM266039.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266040 | GPL339 |
|
Muscle reloaded 15
|
calf muscle, hindlimb suspension, 12 days plus reloading for 3.5hr
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
mice were suspended at approximately a 30° angle by taping the tail to a plastic dowel attached to a swivel apparatus; at 12 days mice were unsuspended for 3.5 hours
|
| Sample_geo_accession | GSM266040
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266040/suppl/GSM266040.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266041 | GPL339 |
|
Muscle reloaded 16
|
calf muscle, hindlimb suspension, 12 days plus reloading for 3.5hr
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
mice were suspended at approximately a 30° angle by taping the tail to a plastic dowel attached to a swivel apparatus; at 12 days mice were unsuspended for 3.5 hours
|
| Sample_geo_accession | GSM266041
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266041/suppl/GSM266041.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266042 | GPL339 |
|
Muscle reloaded 18
|
calf muscle, hindlimb suspension, 12 days plus reloading for 3.5hr
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
mice were suspended at approximately a 30° angle by taping the tail to a plastic dowel attached to a swivel apparatus; at 12 days mice were unsuspended for 3.5 hours
|
| Sample_geo_accession | GSM266042
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266042/suppl/GSM266042.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266043 | GPL339 |
|
Muscle reloaded 33
|
calf muscle, hindlimb suspension, 12 days plus reloading for 3.5hr
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
mice were suspended at approximately a 30° angle by taping the tail to a plastic dowel attached to a swivel apparatus; at 12 days mice were unsuspended for 3.5 hours
|
| Sample_geo_accession | GSM266043
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266043/suppl/GSM266043.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266044 | GPL339 |
|
Muscle suspension 11
|
calf muscle, hindlimb suspension, 12 days
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
mice were suspended at approximately a 30° angle by taping the tail to a plastic dowel attached to a swivel apparatus
|
| Sample_geo_accession | GSM266044
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266044/suppl/GSM266044.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266045 | GPL339 |
|
Muscle suspension 25
|
calf muscle, hindlimb suspension, 12 days
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
mice were suspended at approximately a 30° angle by taping the tail to a plastic dowel attached to a swivel apparatus
|
| Sample_geo_accession | GSM266045
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266045/suppl/GSM266045.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266046 | GPL339 |
|
Muscle suspension 30
|
calf muscle, hindlimb suspension, 12 days
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
mice were suspended at approximately a 30° angle by taping the tail to a plastic dowel attached to a swivel apparatus
|
| Sample_geo_accession | GSM266046
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266046/suppl/GSM266046.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266047 | GPL339 |
|
Muscle suspension 8
|
calf muscle, hindlimb suspension, 12 days
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
mice were suspended at approximately a 30° angle by taping the tail to a plastic dowel attached to a swivel apparatus
|
| Sample_geo_accession | GSM266047
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266047/suppl/GSM266047.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266048 | GPL339 |
|
Muscle suspension 9
|
calf muscle, hindlimb suspension, 12 days
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
mice were suspended at approximately a 30° angle by taping the tail to a plastic dowel attached to a swivel apparatus
|
| Sample_geo_accession | GSM266048
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266048/suppl/GSM266048.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266049 | GPL339 |
|
Muscle AEM 1
|
gastrocnemius, animal enclosure module on earth, 11 days 19hr
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
SF animals were flown on the middeck of the space shuttle Endeavour (STS-108/UF-1) for 11 days and 19 h beginning December 5, 2001
|
| Sample_geo_accession | GSM266049
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266049/suppl/GSM266049.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266050 | GPL339 |
|
Muscle AEM 2
|
gastrocnemius, animal enclosure module on earth, 11 days 19hr
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
SF animals were flown on the middeck of the space shuttle Endeavour (STS-108/UF-1) for 11 days and 19 h beginning December 5, 2001
|
| Sample_geo_accession | GSM266050
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266050/suppl/GSM266050.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266051 | GPL339 |
|
Muscle AEM 3
|
gastrocnemius, animal enclosure module on earth, 11 days 19hr
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
SF animals were flown on the middeck of the space shuttle Endeavour (STS-108/UF-1) for 11 days and 19 h beginning December 5, 2001
|
| Sample_geo_accession | GSM266051
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266051/suppl/GSM266051.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266052 | GPL339 |
|
Muscle AEM 4
|
gastrocnemius, animal enclosure module on earth, 11 days 19hr
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
SF animals were flown on the middeck of the space shuttle Endeavour (STS-108/UF-1) for 11 days and 19 h beginning December 5, 2001
|
| Sample_geo_accession | GSM266052
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266052/suppl/GSM266052.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266053 | GPL339 |
|
Muscle spaceflight 10
|
gastrocnemius, spaceflight, 11 days1 9hr
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
AEM animals were housed at Cape Canaveral Air Force Station’s Hangar L orbital environmental simulator for 11 days and 19 h with conditions mimicking the shuttle’s middeck temperature, humidity, and CO2 levels
|
| Sample_geo_accession | GSM266053
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266053/suppl/GSM266053.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266054 | GPL339 |
|
Muscle spaceflight 11
|
gastrocnemius, spaceflight, 11 days1 9hr
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
AEM animals were housed at Cape Canaveral Air Force Station’s Hangar L orbital environmental simulator for 11 days and 19 h with conditions mimicking the shuttle’s middeck temperature, humidity, and CO2 levels
|
| Sample_geo_accession | GSM266054
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266054/suppl/GSM266054.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266055 | GPL339 |
|
Muscle spaceflight 8
|
gastrocnemius, spaceflight, 11 days1 9hr
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
AEM animals were housed at Cape Canaveral Air Force Station’s Hangar L orbital environmental simulator for 11 days and 19 h with conditions mimicking the shuttle’s middeck temperature, humidity, and CO2 levels
|
| Sample_geo_accession | GSM266055
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266055/suppl/GSM266055.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
| | |
|
GSM266056 | GPL339 |
|
Muscle spaceflight 9
|
gastrocnemius, spaceflight, 11 days1 9hr
|
Strain: C57BL/6J; Gender: female; Age: 77 days
|
AEM animals were housed at Cape Canaveral Air Force Station’s Hangar L orbital environmental simulator for 11 days and 19 h with conditions mimicking the shuttle’s middeck temperature, humidity, and CO2 levels
|
| Sample_geo_accession | GSM266056
| | Sample_status | Public on Dec 28 2008
| | Sample_submission_date | Feb 14 2008
| | Sample_last_update_date | Dec 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | tissues were dissected and then snap frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared from tissue samples by Trizol extraction followed by purification using RNeasy columns (Qiagen Inc., Valencia, CA). Quality and purity of the RNA preparations were assessed by spectrophotometric determination of A260:A280 ratios and by quantification of 28S:18S ribosomal RNA ratios using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesis of double stranded cDNA, in vitro transcription of biotin-labeled cRNA targets and fragmentation of target cRNA were performed as outlined by Affymetrix protocols.
| | Sample_hyb_protocol | Fragmented cRNA samples were hybridized overnight at 45oC in a GeneChip hybridization oven 640. Post hybridization washing and phycoerythrin-streptavidin staining were performed in an Affymetrix Fluidics Station 450 using established Affymetrix protocols.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip Scanner 3000, two passes
| | Sample_data_processing | Expression values were calculated using RMA; detection calls were calculated using MAS5
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jeremy,,Barth
| | Sample_contact_email | barthj@musc.edu
| | Sample_contact_institute | MUSC
| | Sample_contact_address | 173 Ashley Ave, BSB 601
| | Sample_contact_city | Charleston
| | Sample_contact_state | SC
| | Sample_contact_zip/postal_code | 29425
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266056/suppl/GSM266056.CEL.gz
| | Sample_series_id | GSE10533
| | Sample_data_row_count | 22690
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