Search results for the GEO ID: GSE10555
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GSM266738
GPL1261
osteoblast wild type wild type mouse primary osteoblast Strain:129/C57B6, tissue:bone For primary osteoblasts, calvaria from newborn mice were dissected aseptically and digested sequentially in isolation buffer (25mM HEPES pH 7.5,10mM NaHCO3, 100mM NaCl, 3mM K2HPO4, 1mM CaCl2, 30mM KCl, 1mg/ml BSA, 5mg/ml D-glucose) containing 0.75 mg/ml collagenase and 0.2% dispase. Fractions 3–5 were cultured in -MEM containing 10% fetal calf serum (FCS).
GSM266739
GPL1261
osteoblast Slc39a13 knockout knockout mouse primary osteoblast Strain:129/C57B6, tissue:bone, Slc39a13 knock out For primary osteoblasts, calvaria from newborn mice were dissected aseptically and digested sequentially in isolation buffer (25mM HEPES pH 7.5,10mM NaHCO3, 100mM NaCl, 3mM K2HPO4, 1mM CaCl2, 30mM KCl, 1mg/ml BSA, 5mg/ml D-glucose) containing 0.75 mg/ml collagenase and 0.2% dispase. Fractions 3–5 were cultured in -MEM containing 10% fetal calf serum (FCS).
 
 
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