Result

Search results for the GEO ID: GSE10557

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM266747

GPL1355

Postnatal day 4 ovary cultured for 10 days, replicate 1

ovaries dissected from 4-day old Sprague-Dawley rats and cleaned of ovarian bursa and cultured for 10 days

Chip ID=Eric-C-1-070705. RNA from P4-day old rat ovaries cultured for 10 days, replicate 1

Sample_geo_accession	GSM266747
Sample_status	Public on Feb 20 2008
Sample_submission_date	Feb 19 2008
Sample_last_update_date	Feb 19 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Female day P4 rat pups were euthanized and rat ovaries were removed according to IACUC-approved animal use protocols. Ovaries were placed in culture for ten days with no treatment (controls). Whole ovaries were cultured as previously described (Nilsson, Parrott et al. 2001) on floating filters (0.4 um Millicell-CM, Millipore, Bedford, MD) in 0.5 ml Dulbecco's Modified Eagle's Medium (DMEM)-Ham's F-12 medium (1:1, vol/vol) containing 0.1 % bovine serum albumin (BSA, Sigma, St. Louis, MO), 0.1% Albumax (Gibco BRL, Gaithersburg, MD), 27.5 ug/ml transferrin, 200 ug/ml insulin (human recombinant, Sigma), and 0.05 ug/ml L-ascorbic acid (Sigma) in a 4-well culture plate (Nunc plate, Applied Scientific, South San Francisco, CA). Medium was supplemented with penicillin and streptomycin to prevent bacterial contamination.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	About 10 ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturer's instructions (Sigma, St. Louis, MO). Two independent RNA samples were analysed for each treatment group.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix procedures
Sample_hyb_protocol	standard Affymetrix procedure
Sample_scan_protocol	chips were scanned using Agilent GeneArray Scanner G2500A
Sample_data_processing = Auto-scale ON (1-1000). Scaling	all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using a scaling factor of 125, and then imported into the GeneSpring program (Agilent Technologies, Palo Alto, CA).
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266747/suppl/GSM266747.CEL.gz
Sample_series_id	GSE10557
Sample_data_row_count	31099

GSM266748

GPL1355

Postnatal day 4 ovary cultured for 10 days, replicate 2

ovaries dissected from 4-day old Sprague-Dawley rats and cleaned of ovarian bursa and cultured for 10 days

Chip ID=Eric-C-2-070705. RNA from P4-day old rat ovaries cultured for 10 days, replicate 2

Sample_geo_accession	GSM266748
Sample_status	Public on Feb 20 2008
Sample_submission_date	Feb 19 2008
Sample_last_update_date	Feb 19 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Female day P4 rat pups were euthanized and rat ovaries were removed according to IACUC-approved animal use protocols. Ovaries were placed in culture for ten days with no treatment (controls). Whole ovaries were cultured as previously described (Nilsson, Parrott et al. 2001) on floating filters (0.4 um Millicell-CM, Millipore, Bedford, MD) in 0.5 ml Dulbecco's Modified Eagle's Medium (DMEM)-Ham's F-12 medium (1:1, vol/vol) containing 0.1 % bovine serum albumin (BSA, Sigma, St. Louis, MO), 0.1% Albumax (Gibco BRL, Gaithersburg, MD), 27.5 ug/ml transferrin, 200 ug/ml insulin (human recombinant, Sigma), and 0.05 ug/ml L-ascorbic acid (Sigma) in a 4-well culture plate (Nunc plate, Applied Scientific, South San Francisco, CA). Medium was supplemented with penicillin and streptomycin to prevent bacterial contamination.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	About 10 ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturer's instructions (Sigma, St. Louis, MO). Two independent RNA samples were analysed for each treatment group.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix procedures
Sample_hyb_protocol	standard Affymetrix procedure
Sample_scan_protocol	chips were scanned using Agilent GeneArray Scanner G2500A
Sample_data_processing = Auto-scale ON (1-1000). Scaling	all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using a scaling factor of 125, and then imported into the GeneSpring program (Agilent Technologies, Palo Alto, CA).
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266748/suppl/GSM266748.CEL.gz
Sample_series_id	GSE10557
Sample_data_row_count	31099

GSM266749

GPL1355

Postnatal day 4 ovary cultured with AMH for 10 days, replicate 1

ovaries dissected from 4-day old Sprague-Dawley rats and cleaned of ovarian bursa and cultured for 10 days with AMH

Chip ID=Eric-MIS-1-092805. RNA from P4-day old rat ovaries cultured with AMH for 10 days, replicate 1

Sample_geo_accession	GSM266749
Sample_status	Public on Feb 20 2008
Sample_submission_date	Feb 19 2008
Sample_last_update_date	Feb 19 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Female day P4 rat pups were euthanized and rat ovaries were removed according to IACUC-approved animal use protocols. Ovaries were placed in culture for ten days with treatment of 50ng/mL AMH. Whole ovaries were cultured as previously described (Nilsson, Parrott et al. 2001) on floating filters (0.4 um Millicell-CM, Millipore, Bedford, MD) in 0.5 ml Dulbecco's Modified Eagle's Medium (DMEM)-Ham's F-12 medium (1:1, vol/vol) containing 0.1 % bovine serum albumin (BSA, Sigma, St. Louis, MO), 0.1% Albumax (Gibco BRL, Gaithersburg, MD), 27.5 ug/ml transferrin, 200 ug/ml insulin (human recombinant, Sigma), and 0.05 ug/ml L-ascorbic acid (Sigma) in a 4-well culture plate (Nunc plate, Applied Scientific, South San Francisco, CA). Medium was supplemented with penicillin and streptomycin to prevent bacterial contamination.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	About 10 ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturer's instructions (Sigma, St. Louis, MO). Two independent RNA samples were analysed for each treatment group.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix procedures
Sample_hyb_protocol	standard Affymetrix procedure
Sample_scan_protocol	chips were scanned using Agilent GeneArray Scanner G2500A
Sample_data_processing = Auto-scale ON (1-1000). Scaling	all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using a scaling factor of 125, and then imported into the GeneSpring program (Agilent Technologies, Palo Alto, CA).
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266749/suppl/GSM266749.CEL.gz
Sample_series_id	GSE10557
Sample_data_row_count	31099

GSM266750

GPL1355

Postnatal day 4 ovary cultured with AMH for 10 days, replicate 2

ovaries dissected from 4-day old Sprague-Dawley rats and cleaned of ovarian bursa and cultured for 10 days with AMH

Chip ID=Eric-MIS-2-092805. RNA from P4-day old rat ovaries cultured with AMH for 10 days, replicate 2

Sample_geo_accession	GSM266750
Sample_status	Public on Feb 20 2008
Sample_submission_date	Feb 19 2008
Sample_last_update_date	Feb 19 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Female day P4 rat pups were euthanized and rat ovaries were removed according to IACUC-approved animal use protocols. Ovaries were placed in culture for ten days with treatment of 50ng/mL AMH. Whole ovaries were cultured as previously described (Nilsson, Parrott et al. 2001) on floating filters (0.4 um Millicell-CM, Millipore, Bedford, MD) in 0.5 ml Dulbecco's Modified Eagle's Medium (DMEM)-Ham's F-12 medium (1:1, vol/vol) containing 0.1 % bovine serum albumin (BSA, Sigma, St. Louis, MO), 0.1% Albumax (Gibco BRL, Gaithersburg, MD), 27.5 ug/ml transferrin, 200 ug/ml insulin (human recombinant, Sigma), and 0.05 ug/ml L-ascorbic acid (Sigma) in a 4-well culture plate (Nunc plate, Applied Scientific, South San Francisco, CA). Medium was supplemented with penicillin and streptomycin to prevent bacterial contamination.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	About 10 ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturer's instructions (Sigma, St. Louis, MO). Two independent RNA samples were analysed for each treatment group.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix procedures
Sample_hyb_protocol	standard Affymetrix procedure
Sample_scan_protocol	chips were scanned using Agilent GeneArray Scanner G2500A
Sample_data_processing = Auto-scale ON (1-1000). Scaling	all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using a scaling factor of 125, and then imported into the GeneSpring program (Agilent Technologies, Palo Alto, CA).
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266750/suppl/GSM266750.CEL.gz
Sample_series_id	GSE10557
Sample_data_row_count	31099

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