Search results for the GEO ID: GSE10584  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM266842 | GPL96 |
|
Erythroblast_control_day4_rep1B
|
Cultured erythroblast, day 4.
|
Healthy blood donor. Identifier 13C3d4.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor.
|
| Sample_geo_accession | GSM266842
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266842/suppl/GSM266842.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266844 | GPL96 |
|
Erythroblast_control_day6_rep1B
|
Cultured erythroblast, day 6
|
Healthy blood donor. Identifier 13C3d6.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor.
|
| Sample_geo_accession | GSM266844
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266844/suppl/GSM266844.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266846 | GPL96 |
|
Erythroblast_control_day11_rep1B
|
Cultured erythroblast, day 11.
|
Healthy blood donor. Identifier 13C3d11.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor.
|
| Sample_geo_accession | GSM266846
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266846/suppl/GSM266846.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266848 | GPL96 |
|
Erythroblast_InLu_day4_rep1B
|
Cultured erythroblast, In(Lu) phenotype, day 4.
|
Healthy blood donor with the rare blood group In(Lu) phenotype. Identifier 13In3d4.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor with the rare blood group In(Lu) phenotype.
|
| Sample_geo_accession | GSM266848
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266848/suppl/GSM266848.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266850 | GPL96 |
|
Erythroblast_InLu_day6_rep1B
|
Cultured erythroblast, In(Lu) phenotype, day 6.
|
Healthy blood donor with the rare blood group In(Lu) phenotype. Identifier 13In3d6.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor with the rare blood group In(Lu) phenotype.
|
| Sample_geo_accession | GSM266850
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266850/suppl/GSM266850.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266851 | GPL96 |
|
Erythroblast_InLu_day11_rep1B
|
Cultured erythroblast, In(Lu) phenotype, day 11.
|
Healthy blood donor with the rare blood group In(Lu) phenotype. Identifier 13In3d11.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor with the rare blood group In(Lu) phenotype.
|
| Sample_geo_accession | GSM266851
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266851/suppl/GSM266851.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266902 | GPL96 |
|
Erythroblast_control_d6_rep2A
|
Cultured erythroblast, day 6.
|
Healthy blood donor. Identifier 10C4d6.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor.
|
| Sample_geo_accession | GSM266902
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK.
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266902/suppl/GSM266902.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266903 | GPL96 |
|
Erythroblast_control_day11_rep2A
|
Cultured erythroblast, day 11
|
Healthy blood donor. Identifier 10C4d11.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor.
|
| Sample_geo_accession | GSM266903
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK.
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266903/suppl/GSM266903.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266904 | GPL96 |
|
Erythroblast_InLu_day6_rep2A
|
Cultured erythroblast, In(Lu) phenotype, day 6.
|
Healthy blood donor with the rare blood group In(Lu) phenotype. Identifier 10In4d6.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor with the rare blood group In(Lu) phenotype.
|
| Sample_geo_accession | GSM266904
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK.
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266904/suppl/GSM266904.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266905 | GPL96 |
|
Erythroblast_InLu_day11_rep2A
|
Cultured erythroblast, In(Lu) phenotype, day 11.
|
Healthy blood donor with the rare blood group In(Lu) phenotype. Identifier 10In4d11.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor with the rare blood group In(Lu) phenotype.
|
| Sample_geo_accession | GSM266905
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK.
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266905/suppl/GSM266905.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266910 | GPL96 |
|
Erythroblast_control_day6_rep2B
|
Cultured erythroblast, day 6.
|
Healthy blood donor. Identifier 11C4d6.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor.
|
| Sample_geo_accession | GSM266910
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK.
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266910/suppl/GSM266910.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266911 | GPL96 |
|
Erythroblast_control_day11_rep2B
|
Cultured erythroblast, day 11.
|
Healthy blood donor. Identifier 12C4d11.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor.
|
| Sample_geo_accession | GSM266911
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK.
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266911/suppl/GSM266911.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266924 | GPL96 |
|
Erythroblast_InLu_day6_rep2B
|
Cultured erythroblast, In(Lu) phenotype, day 6.
|
Healthy blood donor with the rare blood group In(Lu) phenotype. Identifier 11In4d6.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor with the rare blood group In(Lu) phenotype.
|
| Sample_geo_accession | GSM266924
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK.
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266924/suppl/GSM266924.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266925 | GPL96 |
|
Erythroblast_InLu_day11_rep2B
|
Cultured erythroblast, In(Lu) phenotype, day 11.
|
Healthy blood donor with the rare blood group In(Lu) phenotype. Identifier 11In4d11.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor with the rare blood group In(Lu) phenotype.
|
| Sample_geo_accession | GSM266925
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK.
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266925/suppl/GSM266925.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266926 | GPL96 |
|
Erythroblast_control_day4_rep1A
|
Cultured erythroblast, day 4.
|
Healthy blood donor. Identifier 12C3d4.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor.
|
| Sample_geo_accession | GSM266926
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK.
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266926/suppl/GSM266926.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266927 | GPL96 |
|
Erythroblast_control_day6_rep1A
|
Cultured erythroblast, day 6.
|
Healthy blood donor. Identifier 12C3d6.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor.
|
| Sample_geo_accession | GSM266927
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK.
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266927/suppl/GSM266927.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266928 | GPL96 |
|
Erythroblast_control_day11_rep1A
|
Cultured erythroblast, day 11.
|
Healthy blood donor. Identifier 12C3d11.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor.
|
| Sample_geo_accession | GSM266928
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK.
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266928/suppl/GSM266928.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266929 | GPL96 |
|
Erythroblast_InLu_day4_rep1A
|
Cultured erythroblast, In(Lu) phenotype, day 4.
|
Healthy blood donor with the rare blood group In(Lu) phenotype. Identifier 12In3d4.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor with the rare blood group In(Lu) phenotype.
|
| Sample_geo_accession | GSM266929
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK.
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266929/suppl/GSM266929.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
| | |
|
GSM266930 | GPL96 |
|
Erythroblast_InLu_day6_rep1A
|
Cultured erythroblast, In(Lu) phenotype, day 6.
|
Healthy blood donor with the rare blood group In(Lu) phenotype. Identifier 12In3d6.
|
Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor with the rare blood group In(Lu) phenotype.
|
| Sample_geo_accession | GSM266930
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK.
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266930/suppl/GSM266930.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
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GSM266931 | GPL96 |
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Erythroblast_InLu_day11_rep1A
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Cultured erythroblast, In(Lu) phenotype, day 11.
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Healthy blood donor with the rare blood group In(Lu) phenotype. Identifier 12In3d11.
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Erythroid culture derived from CD34+ cells isolated from the buffy coat of a healthy blood donor with the rare blood group In(Lu) phenotype.
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| Sample_geo_accession | GSM266931
| | Sample_status | Public on Aug 11 2008
| | Sample_submission_date | Feb 20 2008
| | Sample_last_update_date | Aug 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | National Blood Service, Bristol, UK.
| | Sample_growth_protocol_ch1 | CD34+ cells were isolated from peripheral blood buffy coats by positive selection with the MiniMACS magnetic bead system according to the manufacturer’s instructions (Miltenyi Biotec, Bisley, UK). Erythroid progenitor cells were obtained by ex vivo culture of CD34+ cells, as described in the paper by Fricke B et al. Br J Hematol. 2005;131:265-277. Cells were harvested during culture for freezing and storage at -70oC in RNALater (Ambion, Huntingdon, UK).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | A minimum of 1.5 x106 erythroid cells were harvested every few days from day 4 to day 14 of culture and frozen in RNALater (Ambion). Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Crawley, UK), including an on-column DNaseI digestion, according to the manufacturer’s instructions. RNA yield was determined using the RiboGreen RNA Quantitation Kit (Molecular Probes, Invitrogen Ltd, Paisley, UK).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared from 5ug total RNA according to the one-cycle target labelling protocols in the Affymetrix Expression Analysis Technical Manual.
| | Sample_hyb_protocol | Following fragmentation, 15 ug cRNA was hybridised to the Affymetrix HG-U133A chip according to the manufacturer’s protocols. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Scanner 3000.
| | Sample_data_processing | Single Array Expression Analysis was performed using the Affymetrix GeneChip Operating Software (GCOS) with default settings. A global scaling strategy was used to give a target intensity of 500 for each array.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Belinda,,Singleton
| | Sample_contact_institute | Bristol Institute for Transfusion Sciences
| | Sample_contact_address | Southmead Road
| | Sample_contact_city | Bristol
| | Sample_contact_zip/postal_code | BS10 5ND
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM266nnn/GSM266931/suppl/GSM266931.CEL.gz
| | Sample_series_id | GSE10584
| | Sample_data_row_count | 22283
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