Result

Search results for the GEO ID: GSE10591

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM267005

GPL570

HepG2_Tmprss6_rep1

wild type Tmprss6 transfection

human HepG2 cells

Expression data from HepG2 cell line

Sample_geo_accession	GSM267005
Sample_status	Public on Aug 01 2008
Sample_submission_date	Feb 20 2008
Sample_last_update_date	Feb 20 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	HepG2 cells were transfected with wild type Tmprss6 cDNA, mutant version of Tmprss6 and empty vector, respectively for 36 hours.
Sample_growth_protocol_ch1	HepG2 cells were grown in MEM (Invitrogen) supplemented with non-essential amino acids and sodium pyruvate, 10 mM HEPES and 2 mM L-glutamine and 5% FBS and 2% penicillin/streptomycin solution.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by DNase treatment and RNeasy® column clean-up.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was labeled using the MessageAmp II-Biotin Enhanced Single Round RNA Amplification Kit (Perkin-Elmer Inc.)
Sample_hyb_protocol	Samples were hybridized to the Affymetrix Human Genome U133 plus 2.0 array using standard Affymetrix protocols.
Sample_scan_protocol	The chips were scanned using the Affymetrix ScanArray 3000 using default settings.
Sample_data_processing	A target intensity of 250 was used for scaling. Chips were included with a background less than 100 intensity units and a GAPDH 3':5'<3. The raw expression values were normalised using Robust Multichip Average. All further processing of the data were performed within the Bioconductor project and the R program software.
Sample_platform_id	GPL570
Sample_contact_name	Yu,,Xia
Sample_contact_email	yuxia@scripps.edu
Sample_contact_phone	858-784-7364
Sample_contact_laboratory	Bruce Beutler
Sample_contact_department	Genetics
Sample_contact_institute	The Scripps Research Institute
Sample_contact_address	10550, N. Torrey Pines Rd.
Sample_contact_city	La Jolla
Sample_contact_state	CA
Sample_contact_zip/postal_code	92037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267005/suppl/GSM267005.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267005/suppl/GSM267005.EXP.gz
Sample_series_id	GSE10591
Sample_data_row_count	54675

GSM267006

GPL570

HepG2_Tmprss6_rep2

wild type Tmprss6 transfection

human HepG2 cells

Expression data from HepG2 cell line

Sample_geo_accession	GSM267006
Sample_status	Public on Aug 01 2008
Sample_submission_date	Feb 20 2008
Sample_last_update_date	Feb 20 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	HepG2 cells were transfected with wild type Tmprss6 cDNA, mutant version of Tmprss6 and empty vector, respectively for 36 hours.
Sample_growth_protocol_ch1	HepG2 cells were grown in MEM (Invitrogen) supplemented with non-essential amino acids and sodium pyruvate, 10 mM HEPES and 2 mM L-glutamine and 5% FBS and 2% penicillin/streptomycin solution.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by DNase treatment and RNeasy® column clean-up.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was labeled using the MessageAmp II-Biotin Enhanced Single Round RNA Amplification Kit (Perkin-Elmer Inc.)
Sample_hyb_protocol	Samples were hybridized to the Affymetrix Human Genome U133 plus 2.0 array using standard Affymetrix protocols.
Sample_scan_protocol	The chips were scanned using the Affymetrix ScanArray 3000 using default settings.
Sample_data_processing	A target intensity of 250 was used for scaling. Chips were included with a background less than 100 intensity units and a GAPDH 3':5'<3. The raw expression values were normalised using Robust Multichip Average. All further processing of the data were performed within the Bioconductor project and the R program software.
Sample_platform_id	GPL570
Sample_contact_name	Yu,,Xia
Sample_contact_email	yuxia@scripps.edu
Sample_contact_phone	858-784-7364
Sample_contact_laboratory	Bruce Beutler
Sample_contact_department	Genetics
Sample_contact_institute	The Scripps Research Institute
Sample_contact_address	10550, N. Torrey Pines Rd.
Sample_contact_city	La Jolla
Sample_contact_state	CA
Sample_contact_zip/postal_code	92037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267006/suppl/GSM267006.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267006/suppl/GSM267006.EXP.gz
Sample_series_id	GSE10591
Sample_data_row_count	54675

GSM267007

GPL570

HepG2_Tmprss6_rep3

wild type Tmprss6 transfection

human HepG2 cells

Expression data from HepG2 cell line

Sample_geo_accession	GSM267007
Sample_status	Public on Aug 01 2008
Sample_submission_date	Feb 20 2008
Sample_last_update_date	Feb 20 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	HepG2 cells were transfected with wild type Tmprss6 cDNA, mutant version of Tmprss6 and empty vector, respectively for 36 hours.
Sample_growth_protocol_ch1	HepG2 cells were grown in MEM (Invitrogen) supplemented with non-essential amino acids and sodium pyruvate, 10 mM HEPES and 2 mM L-glutamine and 5% FBS and 2% penicillin/streptomycin solution.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by DNase treatment and RNeasy® column clean-up.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was labeled using the MessageAmp II-Biotin Enhanced Single Round RNA Amplification Kit (Perkin-Elmer Inc.)
Sample_hyb_protocol	Samples were hybridized to the Affymetrix Human Genome U133 plus 2.0 array using standard Affymetrix protocols.
Sample_scan_protocol	The chips were scanned using the Affymetrix ScanArray 3000 using default settings.
Sample_data_processing	A target intensity of 250 was used for scaling. Chips were included with a background less than 100 intensity units and a GAPDH 3':5'<3. The raw expression values were normalised using Robust Multichip Average. All further processing of the data were performed within the Bioconductor project and the R program software.
Sample_platform_id	GPL570
Sample_contact_name	Yu,,Xia
Sample_contact_email	yuxia@scripps.edu
Sample_contact_phone	858-784-7364
Sample_contact_laboratory	Bruce Beutler
Sample_contact_department	Genetics
Sample_contact_institute	The Scripps Research Institute
Sample_contact_address	10550, N. Torrey Pines Rd.
Sample_contact_city	La Jolla
Sample_contact_state	CA
Sample_contact_zip/postal_code	92037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267007/suppl/GSM267007.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267007/suppl/GSM267007.EXP.gz
Sample_series_id	GSE10591
Sample_data_row_count	54675

GSM267008

GPL570

HepG2_mutant Tmprss6_rep1

mutant Tmprss6 transfection

human HepG2 cells

Expression data from HepG2 cell line

Sample_geo_accession	GSM267008
Sample_status	Public on Aug 01 2008
Sample_submission_date	Feb 20 2008
Sample_last_update_date	Feb 20 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	HepG2 cells were transfected with wild type Tmprss6 cDNA, mutant version of Tmprss6 and empty vector, respectively for 36 hours.
Sample_growth_protocol_ch1	HepG2 cells were grown in MEM (Invitrogen) supplemented with non-essential amino acids and sodium pyruvate, 10 mM HEPES and 2 mM L-glutamine and 5% FBS and 2% penicillin/streptomycin solution.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by DNase treatment and RNeasy® column clean-up.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was labeled using the MessageAmp II-Biotin Enhanced Single Round RNA Amplification Kit (Perkin-Elmer Inc.)
Sample_hyb_protocol	Samples were hybridized to the Affymetrix Human Genome U133 plus 2.0 array using standard Affymetrix protocols.
Sample_scan_protocol	The chips were scanned using the Affymetrix ScanArray 3000 using default settings.
Sample_data_processing	A target intensity of 250 was used for scaling. Chips were included with a background less than 100 intensity units and a GAPDH 3':5'<3. The raw expression values were normalised using Robust Multichip Average. All further processing of the data were performed within the Bioconductor project and the R program software.
Sample_platform_id	GPL570
Sample_contact_name	Yu,,Xia
Sample_contact_email	yuxia@scripps.edu
Sample_contact_phone	858-784-7364
Sample_contact_laboratory	Bruce Beutler
Sample_contact_department	Genetics
Sample_contact_institute	The Scripps Research Institute
Sample_contact_address	10550, N. Torrey Pines Rd.
Sample_contact_city	La Jolla
Sample_contact_state	CA
Sample_contact_zip/postal_code	92037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267008/suppl/GSM267008.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267008/suppl/GSM267008.EXP.gz
Sample_series_id	GSE10591
Sample_data_row_count	54675

GSM267009

GPL570

HepG2_mutant Tmprss6_rep2

mutant Tmprss6 transfection

human HepG2 cells

Expression data from HepG2 cell line

Sample_geo_accession	GSM267009
Sample_status	Public on Aug 01 2008
Sample_submission_date	Feb 20 2008
Sample_last_update_date	Feb 20 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	HepG2 cells were transfected with wild type Tmprss6 cDNA, mutant version of Tmprss6 and empty vector, respectively for 36 hours.
Sample_growth_protocol_ch1	HepG2 cells were grown in MEM (Invitrogen) supplemented with non-essential amino acids and sodium pyruvate, 10 mM HEPES and 2 mM L-glutamine and 5% FBS and 2% penicillin/streptomycin solution.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by DNase treatment and RNeasy® column clean-up.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was labeled using the MessageAmp II-Biotin Enhanced Single Round RNA Amplification Kit (Perkin-Elmer Inc.)
Sample_hyb_protocol	Samples were hybridized to the Affymetrix Human Genome U133 plus 2.0 array using standard Affymetrix protocols.
Sample_scan_protocol	The chips were scanned using the Affymetrix ScanArray 3000 using default settings.
Sample_data_processing	A target intensity of 250 was used for scaling. Chips were included with a background less than 100 intensity units and a GAPDH 3':5'<3. The raw expression values were normalised using Robust Multichip Average. All further processing of the data were performed within the Bioconductor project and the R program software.
Sample_platform_id	GPL570
Sample_contact_name	Yu,,Xia
Sample_contact_email	yuxia@scripps.edu
Sample_contact_phone	858-784-7364
Sample_contact_laboratory	Bruce Beutler
Sample_contact_department	Genetics
Sample_contact_institute	The Scripps Research Institute
Sample_contact_address	10550, N. Torrey Pines Rd.
Sample_contact_city	La Jolla
Sample_contact_state	CA
Sample_contact_zip/postal_code	92037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267009/suppl/GSM267009.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267009/suppl/GSM267009.EXP.gz
Sample_series_id	GSE10591
Sample_data_row_count	54675

GSM267010

GPL570

HepG2_mutant Tmprss6_rep3

mutant Tmprss6 transfection

human HepG2 cells

Expression data from HepG2 cell line

Sample_geo_accession	GSM267010
Sample_status	Public on Aug 01 2008
Sample_submission_date	Feb 20 2008
Sample_last_update_date	Feb 20 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	HepG2 cells were transfected with wild type Tmprss6 cDNA, mutant version of Tmprss6 and empty vector, respectively for 36 hours.
Sample_growth_protocol_ch1	HepG2 cells were grown in MEM (Invitrogen) supplemented with non-essential amino acids and sodium pyruvate, 10 mM HEPES and 2 mM L-glutamine and 5% FBS and 2% penicillin/streptomycin solution.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by DNase treatment and RNeasy® column clean-up.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was labeled using the MessageAmp II-Biotin Enhanced Single Round RNA Amplification Kit (Perkin-Elmer Inc.)
Sample_hyb_protocol	Samples were hybridized to the Affymetrix Human Genome U133 plus 2.0 array using standard Affymetrix protocols.
Sample_scan_protocol	The chips were scanned using the Affymetrix ScanArray 3000 using default settings.
Sample_data_processing	A target intensity of 250 was used for scaling. Chips were included with a background less than 100 intensity units and a GAPDH 3':5'<3. The raw expression values were normalised using Robust Multichip Average. All further processing of the data were performed within the Bioconductor project and the R program software.
Sample_platform_id	GPL570
Sample_contact_name	Yu,,Xia
Sample_contact_email	yuxia@scripps.edu
Sample_contact_phone	858-784-7364
Sample_contact_laboratory	Bruce Beutler
Sample_contact_department	Genetics
Sample_contact_institute	The Scripps Research Institute
Sample_contact_address	10550, N. Torrey Pines Rd.
Sample_contact_city	La Jolla
Sample_contact_state	CA
Sample_contact_zip/postal_code	92037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267010/suppl/GSM267010.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267010/suppl/GSM267010.EXP.gz
Sample_series_id	GSE10591
Sample_data_row_count	54675

GSM267011

GPL570

HepG2_Vector_rep1

empty vector transfection

human HepG2 cells

Expression data from HepG2 cell line

Sample_geo_accession	GSM267011
Sample_status	Public on Aug 01 2008
Sample_submission_date	Feb 20 2008
Sample_last_update_date	Feb 20 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	HepG2 cells were transfected with wild type Tmprss6 cDNA, mutant version of Tmprss6 and empty vector, respectively for 36 hours.
Sample_growth_protocol_ch1	HepG2 cells were grown in MEM (Invitrogen) supplemented with non-essential amino acids and sodium pyruvate, 10 mM HEPES and 2 mM L-glutamine and 5% FBS and 2% penicillin/streptomycin solution.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by DNase treatment and RNeasy® column clean-up.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was labeled using the MessageAmp II-Biotin Enhanced Single Round RNA Amplification Kit (Perkin-Elmer Inc.)
Sample_hyb_protocol	Samples were hybridized to the Affymetrix Human Genome U133 plus 2.0 array using standard Affymetrix protocols.
Sample_scan_protocol	The chips were scanned using the Affymetrix ScanArray 3000 using default settings.
Sample_data_processing	A target intensity of 250 was used for scaling. Chips were included with a background less than 100 intensity units and a GAPDH 3':5'<3. The raw expression values were normalised using Robust Multichip Average. All further processing of the data were performed within the Bioconductor project and the R program software.
Sample_platform_id	GPL570
Sample_contact_name	Yu,,Xia
Sample_contact_email	yuxia@scripps.edu
Sample_contact_phone	858-784-7364
Sample_contact_laboratory	Bruce Beutler
Sample_contact_department	Genetics
Sample_contact_institute	The Scripps Research Institute
Sample_contact_address	10550, N. Torrey Pines Rd.
Sample_contact_city	La Jolla
Sample_contact_state	CA
Sample_contact_zip/postal_code	92037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267011/suppl/GSM267011.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267011/suppl/GSM267011.EXP.gz
Sample_series_id	GSE10591
Sample_data_row_count	54675

GSM267012

GPL570

HepG2_Vector_rep2

empty vector transfection

human HepG2 cells

Expression data from HepG2 cell line

Sample_geo_accession	GSM267012
Sample_status	Public on Aug 01 2008
Sample_submission_date	Feb 20 2008
Sample_last_update_date	Feb 20 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	HepG2 cells were transfected with wild type Tmprss6 cDNA, mutant version of Tmprss6 and empty vector, respectively for 36 hours.
Sample_growth_protocol_ch1	HepG2 cells were grown in MEM (Invitrogen) supplemented with non-essential amino acids and sodium pyruvate, 10 mM HEPES and 2 mM L-glutamine and 5% FBS and 2% penicillin/streptomycin solution.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by DNase treatment and RNeasy® column clean-up.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	RNA was labeled using the MessageAmp II-Biotin Enhanced Single Round RNA Amplification Kit (Perkin-Elmer Inc.)
Sample_hyb_protocol	Samples were hybridized to the Affymetrix Human Genome U133 plus 2.0 array using standard Affymetrix protocols.
Sample_scan_protocol	The chips were scanned using the Affymetrix ScanArray 3000 using default settings.
Sample_data_processing	A target intensity of 250 was used for scaling. Chips were included with a background less than 100 intensity units and a GAPDH 3':5'<3. The raw expression values were normalised using Robust Multichip Average. All further processing of the data were performed within the Bioconductor project and the R program software.
Sample_platform_id	GPL570
Sample_contact_name	Yu,,Xia
Sample_contact_email	yuxia@scripps.edu
Sample_contact_phone	858-784-7364
Sample_contact_laboratory	Bruce Beutler
Sample_contact_department	Genetics
Sample_contact_institute	The Scripps Research Institute
Sample_contact_address	10550, N. Torrey Pines Rd.
Sample_contact_city	La Jolla
Sample_contact_state	CA
Sample_contact_zip/postal_code	92037
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267012/suppl/GSM267012.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267012/suppl/GSM267012.EXP.gz
Sample_series_id	GSE10591
Sample_data_row_count	54675

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