Search results for the GEO ID: GSE10615  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM267499 | GPL96 |
|
Germ Cell Tumor, Histology 1, Biological replicate 1
|
Seminoma (Dysgerminoma)
|
Pure, ovarian, female, age 10, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267499
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267499/suppl/GSM267499.cel.gz
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267500 | GPL96 |
|
Germ Cell Tumor, Histology 1, Biological replicate 2
|
Seminoma (Dysgerminoma)
|
Pure, ovarian, female, age 12, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267500
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267500/suppl/GSM267500.CEL.gz
| | Sample_relation | Reanalyzed by: GSM453860
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267501 | GPL96 |
|
Germ Cell Tumor, Histology 1, Biological replicate 3
|
Seminoma (Dysgerminoma)
|
Pure, ovarian, female, age 12, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267501
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267501/suppl/GSM267501.CEL.gz
| | Sample_relation | Reanalyzed by: GSM453856
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267502 | GPL96 |
|
Germ Cell Tumor, Histology 1, Biological replicate 4
|
Seminoma (Dysgerminoma)
|
Pure, ovarian, female, age 12, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267502
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267502/suppl/GSM267502.cel.gz
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267503 | GPL96 |
|
Germ Cell Tumor, Histology 1, Biological replicate 5
|
Seminoma (Dysgerminoma)
|
Pure, ovarian, female, age 13, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267503
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267503/suppl/GSM267503.cel.gz
| | Sample_relation | Reanalyzed by: GSM453861
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267504 | GPL96 |
|
Germ Cell Tumor, Histology 1, Biological replicate 6
|
Seminoma (Dysgerminoma)
|
Pure, ovarian, female, age 13, stage 3
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267504
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267504/suppl/GSM267504.CEL.gz
| | Sample_relation | Reanalyzed by: GSM453859
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267505 | GPL96 |
|
Germ Cell Tumor, Histology 1, Biological replicate 7
|
Seminoma (Germinoma)
|
Pure, CNS, female, age 10, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267505
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267505/suppl/GSM267505.cel.gz
| | Sample_relation | Reanalyzed by: GSM453857
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267506 | GPL96 |
|
Germ Cell Tumor, Histology 1, Biological replicate 8
|
Seminoma (Germinoma)
|
Pure, CNS, male, age 16, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267506
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267506/suppl/GSM267506.cel.gz
| | Sample_relation | Reanalyzed by: GSM453858
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267507 | GPL96 |
|
Germ Cell Tumor, Histology 1, Biological replicate 9
|
Seminoma (Dysgerminoma)
|
Within teratoma, ovarian, female, age 12, stage 2
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267507
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267507/suppl/GSM267507.cel.gz
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267508 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 1
|
Yolk sac tumor
|
Pure, testicular, male, age 0, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267508
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267508/suppl/GSM267508.CEL.gz
| | Sample_relation | Reanalyzed by: GSM453852
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267509 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 2
|
Yolk sac tumor
|
Pure, testicular, male, age 0, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267509
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267509/suppl/GSM267509.CEL.gz
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267510 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 3
|
Yolk sac tumor
|
Pure, testicular, male, age 1, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267510
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267510/suppl/GSM267510.CEL.gz
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267511 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 4
|
Yolk sac tumor
|
Pure, testicular, male, age 1, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267511
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267511/suppl/GSM267511.cel.gz
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267512 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 5
|
Yolk sac tumor
|
Pure, testicular, male, age 1, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267512
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267512/suppl/GSM267512.CEL.gz
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267513 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 6
|
Yolk sac tumor
|
Pure, testicular, male, age 4, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267513
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267513/suppl/GSM267513.cel.gz
| | Sample_relation | Reanalyzed by: GSM453848
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267514 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 7
|
Yolk sac tumor
|
Pure, testicular, male, age 2, stage 2
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267514
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267514/suppl/GSM267514.cel.gz
| | Sample_relation | Reanalyzed by: GSM453851
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267515 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 8
|
Yolk sac tumor
|
Pure, ovarian, female, age 0, stage 2
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267515
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267515/suppl/GSM267515.CEL.gz
| | Sample_relation | Reanalyzed by: GSM453854
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267516 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 9
|
Yolk sac tumor
|
Pure, ovarian, female, age 9, stage 2
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267516
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267516/suppl/GSM267516.cel.gz
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267517 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 10
|
Yolk sac tumor
|
Pure, ovarian, female, age 12, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267517
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267517/suppl/GSM267517.CEL.gz
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267518 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 11
|
Yolk sac tumor
|
Pure, ovarian, female, age 12, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267518
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267518/suppl/GSM267518.cel.gz
| | Sample_relation | Reanalyzed by: GSM453850
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267519 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 12
|
Yolk sac tumor
|
Pure, ovarian, female, age 13, stage 2
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267519
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267519/suppl/GSM267519.CEL.gz
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267520 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 13
|
Yolk sac tumor
|
Pure, ovarian, female, age 13, stage 3
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267520
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267520/suppl/GSM267520.cel.gz
| | Sample_relation | Reanalyzed by: GSM453849
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267521 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 14
|
Yolk sac tumor
|
Pure, ovarian, female, age 14, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267521
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267521/suppl/GSM267521.CEL.gz
| | Sample_relation | Reanalyzed by: GSM453855
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267522 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 15
|
Yolk sac tumor
|
Pure, ovarian, female, age 14, stage 4
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267522
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267522/suppl/GSM267522.CEL.gz
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267523 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 16
|
Yolk sac tumor
|
Within teratoma, SCT, female, age 2, stage 4
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267523
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267523/suppl/GSM267523.cel.gz
| | Sample_relation | Reanalyzed by: GSM453853
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267524 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 17
|
Yolk sac tumor
|
Within teratoma, SCT, female, age 3, stage 5
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267524
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267524/suppl/GSM267524.cel.gz
| | Sample_relation | Reanalyzed by: GSM453847
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
GSM267525 | GPL96 |
|
Germ Cell Tumour, Histology 2, Biological replicate 18
|
Yolk sac tumor
|
Within teratoma, CNS, male, age 12, stage 1
|
Gene expression data from malignant germ cell tumor
|
| Sample_geo_accession | GSM267525
| | Sample_status | Public on May 31 2008
| | Sample_submission_date | Feb 22 2008
| | Sample_last_update_date | Apr 24 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | N/A
| | Sample_growth_protocol_ch1 | Fresh frozen tumor material (pre-chemotherapy) was provided by the individual banking centers across the UK and from Düsseldorf, Germany
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA from each sample was used to prepare biotinylated target RNA in a one-cycle target labeling procedure, with minor modifications from the manufacturer’s recommendations available at: http://www.affymetrix.com/support/technical/manual/expression_manual.affx. Briefly, 10 µg of cleaned total RNA was used to generate first-strand cDNA by using a T7-linked oligo(dT) primer. After second-strand synthesis, in vitro transcription was performed with biotinylated UTP and CTP (Enzo Diagnostics), resulting in approximately 10-fold amplification of RNA.
| | Sample_hyb_protocol | Target cRNA generated from each sample were then processed as per manufacturer's recommendation using an Affymetrix GeneChip Instrument System (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) at Geneservice Ltd (Cambridge Science Park, Cambridge, UK), formerly MRC Geneservice. Briefly, spike controls were added to 15 µg fragmented cRNA before overnight hybridization with the Genechip Human Genome U133A arrays (Affymetrix).
| | Sample_scan_protocol | Arrays were then washed and stained with streptavidin-phycoerythrin, before being scanned on an Affymetrix GeneChip scanner.
| | Sample_data_processing | The array, once scanned, was used to generate an image file (with the extension .dat). GeneChip Operating Software (GCOS) was then used to compute cell intensity data from the image file and this data was saved in a file with the extension .cel. The .cel (CEL) files were provided by Geneservice Ltd and used for subsequent data analysis. Pre-processing of microarray data (including background correction and normalization) relied on the Robust Multi-Array Average (RMA) method within R.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Roger,David,Palmer
| | Sample_contact_email | rdp@hutchison-mrc.cam.ac.uk
| | Sample_contact_phone | 0044 1223 763279
| | Sample_contact_fax | 0044 1223 763284
| | Sample_contact_laboratory | 2.5
| | Sample_contact_department | MRC Cancer Cell Unit
| | Sample_contact_institute | Hutchison/MRC Research Centre
| | Sample_contact_address | Box 197 Hills Road
| | Sample_contact_city | Cambridge
| | Sample_contact_zip/postal_code | CB2 0XZ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM267nnn/GSM267525/suppl/GSM267525.cel.gz
| | Sample_relation | Reanalyzed by: GSM453846
| | Sample_relation | Reanalyzed by: GSE18155
| | Sample_series_id | GSE10615
| | Sample_data_row_count | 22283
| | |
|
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