Sample_geo_accession | GSM269935
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Sample_status | Public on Mar 25 2008
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Sample_submission_date | Feb 29 2008
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Sample_last_update_date | Mar 23 2009
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Mus musculus
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Sample_taxid_ch1 | 10090
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Sample_growth_protocol_ch1 | All cell lines were maintained in DMEM (Gibco/InVitrogen) containing 10% fetal bovine serum (FBS).
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Sample_label_ch1 | biotin
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Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneGPL339 (Affymetrix GeneChip Mouse Expression Array 430A and Mouse Genome 430A 2.0 Array).
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Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
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Sample_data_processing | Initial scaling was done using the Affymetrix Microarray Suite Expression Software version 5.0 and subsequent analysis was done using GeneSpring Software version 6.1 ( Silicon Genetics).
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Sample_platform_id | GPL1261
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Sample_contact_name | Cristina,M,Karp
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Sample_contact_email | karp@cabm.rutgers.edu
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Sample_contact_institute | Rutgers University
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Sample_contact_address | 679 Hoes lane
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Sample_contact_city | Piscataway
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Sample_contact_state | NJ
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Sample_contact_zip/postal_code | NJ 08854
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Sample_contact_country | USA
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM269nnn/GSM269935/suppl/GSM269935.CEL.gz
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Sample_series_id | GSE10682
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Sample_data_row_count | 22690
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