Search results for the GEO ID: GSE10685  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM269808 | GPL570 |
|
Subject CO, 0h
|
Human skeletal muscle biopsy from a 3h IL-6 infusion
|
Subject: CO
Timepoint: 0h
Sex: Male
|
Human skeletal muscle biopsy from vastus lateralis of musculus quadriceps muscle from a male subject.
|
| Sample_geo_accession | GSM269808
| | Sample_status | Public on May 28 2008
| | Sample_submission_date | Feb 29 2008
| | Sample_last_update_date | May 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | IL-6 infusion from 0h to 3h
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol, followed by RNEasy (Qiagen) purification of total RNA
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was synthesized to double-strand cDNA using Superscript Choice System (Invitrogen, Carlsbad, CA, USA) with an oligo-dT primer containing a T7 RNA polymerase promoter (GenSet, Evry, France). The cDNA was used as a template for in vitro transcription reaction to synthesize biotin-labeled antisense cRNA (BioArray High Yield RNA Transcript Labeling Kit; Enzo Diagnostics, Farmingdale, NY, USA)
| | Sample_hyb_protocol | After fragmentation at 94 C for 35 min in fragmentation buffer (40 mM Tris, 30 mM MgOAc, 10 mM KOAc), the labeled cRNA was hybridized for 16 h to Affymetrix HG-U133 Plus 2.0 (Affymetrix Inc., Santa Clara, CA, USA).After hybridization the array was washed and stained with streptavidin phycoerythrin solution using the Fluidics Station 400 (Affymetrix Inc.).
| | Sample_scan_protocol | Finally the arrays were scanned using the GeneChip Scanner 3000 (Affymetrix Inc.) to obtain non-normalized expression levels.
| | Sample_data_processing | Affymetrix CEL-files were normalized using gcRMA (Wu et al., 2004) available in the Bioconductor package version 1.7 (http://www.bioconductor.org) for the statistical software package R version 2.21 (R Development Core Team, 2005).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ole,Hartvig,Mortensen
| | Sample_contact_email | ole@hartvig.org
| | Sample_contact_laboratory | Cellular and Metabolic Research Section
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Copenhagen
| | Sample_contact_address | Panum 6.5.8, Blegdamsvej 3
| | Sample_contact_city | Copenhagen N
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM269nnn/GSM269808/suppl/GSM269808.CEL.gz
| | Sample_series_id | GSE10685
| | Sample_data_row_count | 54675
| | |
|
GSM269809 | GPL570 |
|
Subject CO, 3h
|
Human skeletal muscle biopsy from a 3h IL-6 infusion
|
Subject: CO
Timepoint: 3h
Sex: Male
|
Human skeletal muscle biopsy from vastus lateralis of musculus quadriceps muscle from a male subject.
|
| Sample_geo_accession | GSM269809
| | Sample_status | Public on May 28 2008
| | Sample_submission_date | Feb 29 2008
| | Sample_last_update_date | May 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | IL-6 infusion from 0h to 3h
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol, followed by RNEasy (Qiagen) purification of total RNA
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was synthesized to double-strand cDNA using Superscript Choice System (Invitrogen, Carlsbad, CA, USA) with an oligo-dT primer containing a T7 RNA polymerase promoter (GenSet, Evry, France). The cDNA was used as a template for in vitro transcription reaction to synthesize biotin-labeled antisense cRNA (BioArray High Yield RNA Transcript Labeling Kit; Enzo Diagnostics, Farmingdale, NY, USA)
| | Sample_hyb_protocol | After fragmentation at 94 C for 35 min in fragmentation buffer (40 mM Tris, 30 mM MgOAc, 10 mM KOAc), the labeled cRNA was hybridized for 16 h to Affymetrix HG-U133 Plus 2.0 (Affymetrix Inc., Santa Clara, CA, USA).After hybridization the array was washed and stained with streptavidin phycoerythrin solution using the Fluidics Station 400 (Affymetrix Inc.).
| | Sample_scan_protocol | Finally the arrays were scanned using the GeneChip Scanner 3000 (Affymetrix Inc.) to obtain non-normalized expression levels.
| | Sample_data_processing | Affymetrix CEL-files were normalized using gcRMA (Wu et al., 2004) available in the Bioconductor package version 1.7 (http://www.bioconductor.org) for the statistical software package R version 2.21 (R Development Core Team, 2005).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ole,Hartvig,Mortensen
| | Sample_contact_email | ole@hartvig.org
| | Sample_contact_laboratory | Cellular and Metabolic Research Section
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Copenhagen
| | Sample_contact_address | Panum 6.5.8, Blegdamsvej 3
| | Sample_contact_city | Copenhagen N
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM269nnn/GSM269809/suppl/GSM269809.CEL.gz
| | Sample_series_id | GSE10685
| | Sample_data_row_count | 54675
| | |
|
GSM269810 | GPL570 |
|
Subject CO, 6h
|
Human skeletal muscle biopsy from a 3h IL-6 infusion
|
Subject: CO
Timepoint: 6h
Sex: Male
|
Human skeletal muscle biopsy from vastus lateralis of musculus quadriceps muscle from a male subject.
|
| Sample_geo_accession | GSM269810
| | Sample_status | Public on May 28 2008
| | Sample_submission_date | Feb 29 2008
| | Sample_last_update_date | May 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | IL-6 infusion from 0h to 3h
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol, followed by RNEasy (Qiagen) purification of total RNA
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was synthesized to double-strand cDNA using Superscript Choice System (Invitrogen, Carlsbad, CA, USA) with an oligo-dT primer containing a T7 RNA polymerase promoter (GenSet, Evry, France). The cDNA was used as a template for in vitro transcription reaction to synthesize biotin-labeled antisense cRNA (BioArray High Yield RNA Transcript Labeling Kit; Enzo Diagnostics, Farmingdale, NY, USA)
| | Sample_hyb_protocol | After fragmentation at 94 C for 35 min in fragmentation buffer (40 mM Tris, 30 mM MgOAc, 10 mM KOAc), the labeled cRNA was hybridized for 16 h to Affymetrix HG-U133 Plus 2.0 (Affymetrix Inc., Santa Clara, CA, USA).After hybridization the array was washed and stained with streptavidin phycoerythrin solution using the Fluidics Station 400 (Affymetrix Inc.).
| | Sample_scan_protocol | Finally the arrays were scanned using the GeneChip Scanner 3000 (Affymetrix Inc.) to obtain non-normalized expression levels.
| | Sample_data_processing | Affymetrix CEL-files were normalized using gcRMA (Wu et al., 2004) available in the Bioconductor package version 1.7 (http://www.bioconductor.org) for the statistical software package R version 2.21 (R Development Core Team, 2005).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ole,Hartvig,Mortensen
| | Sample_contact_email | ole@hartvig.org
| | Sample_contact_laboratory | Cellular and Metabolic Research Section
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Copenhagen
| | Sample_contact_address | Panum 6.5.8, Blegdamsvej 3
| | Sample_contact_city | Copenhagen N
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM269nnn/GSM269810/suppl/GSM269810.CEL.gz
| | Sample_series_id | GSE10685
| | Sample_data_row_count | 54675
| | |
|
GSM269811 | GPL570 |
|
Subject DP, 0h
|
Human skeletal muscle biopsy from a 3h IL-6 infusion
|
Subject: DP
Timepoint: 0h
Sex: Male
|
Human skeletal muscle biopsy from vastus lateralis of musculus quadriceps muscle from a male subject.
|
| Sample_geo_accession | GSM269811
| | Sample_status | Public on May 28 2008
| | Sample_submission_date | Feb 29 2008
| | Sample_last_update_date | May 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | IL-6 infusion from 0h to 3h
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol, followed by RNEasy (Qiagen) purification of total RNA
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was synthesized to double-strand cDNA using Superscript Choice System (Invitrogen, Carlsbad, CA, USA) with an oligo-dT primer containing a T7 RNA polymerase promoter (GenSet, Evry, France). The cDNA was used as a template for in vitro transcription reaction to synthesize biotin-labeled antisense cRNA (BioArray High Yield RNA Transcript Labeling Kit; Enzo Diagnostics, Farmingdale, NY, USA)
| | Sample_hyb_protocol | After fragmentation at 94 C for 35 min in fragmentation buffer (40 mM Tris, 30 mM MgOAc, 10 mM KOAc), the labeled cRNA was hybridized for 16 h to Affymetrix HG-U133 Plus 2.0 (Affymetrix Inc., Santa Clara, CA, USA).After hybridization the array was washed and stained with streptavidin phycoerythrin solution using the Fluidics Station 400 (Affymetrix Inc.).
| | Sample_scan_protocol | Finally the arrays were scanned using the GeneChip Scanner 3000 (Affymetrix Inc.) to obtain non-normalized expression levels.
| | Sample_data_processing | Affymetrix CEL-files were normalized using gcRMA (Wu et al., 2004) available in the Bioconductor package version 1.7 (http://www.bioconductor.org) for the statistical software package R version 2.21 (R Development Core Team, 2005).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ole,Hartvig,Mortensen
| | Sample_contact_email | ole@hartvig.org
| | Sample_contact_laboratory | Cellular and Metabolic Research Section
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Copenhagen
| | Sample_contact_address | Panum 6.5.8, Blegdamsvej 3
| | Sample_contact_city | Copenhagen N
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM269nnn/GSM269811/suppl/GSM269811.CEL.gz
| | Sample_series_id | GSE10685
| | Sample_data_row_count | 54675
| | |
|
GSM269834 | GPL570 |
|
Subject DP, 3h
|
Human skeletal muscle biopsy from a 3h IL-6 infusion
|
Subject: DP
Timepoint: 3h
Sex: Male
|
Human skeletal muscle biopsy from vastus lateralis of musculus quadriceps muscle from a male subject.
|
| Sample_geo_accession | GSM269834
| | Sample_status | Public on May 28 2008
| | Sample_submission_date | Feb 29 2008
| | Sample_last_update_date | May 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | IL-6 infusion from 0h to 3h
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol, followed by RNEasy (Qiagen) purification of total RNA
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was synthesized to double-strand cDNA using Superscript Choice System (Invitrogen, Carlsbad, CA, USA) with an oligo-dT primer containing a T7 RNA polymerase promoter (GenSet, Evry, France). The cDNA was used as a template for in vitro transcription reaction to synthesize biotin-labeled antisense cRNA (BioArray High Yield RNA Transcript Labeling Kit; Enzo Diagnostics, Farmingdale, NY, USA)
| | Sample_hyb_protocol | After fragmentation at 94 C for 35 min in fragmentation buffer (40 mM Tris, 30 mM MgOAc, 10 mM KOAc), the labeled cRNA was hybridized for 16 h to Affymetrix HG-U133 Plus 2.0 (Affymetrix Inc., Santa Clara, CA, USA).After hybridization the array was washed and stained with streptavidin phycoerythrin solution using the Fluidics Station 400 (Affymetrix Inc.).
| | Sample_scan_protocol | Finally the arrays were scanned using the GeneChip Scanner 3000 (Affymetrix Inc.) to obtain non-normalized expression levels.
| | Sample_data_processing | Affymetrix CEL-files were normalized using gcRMA (Wu et al., 2004) available in the Bioconductor package version 1.7 (http://www.bioconductor.org) for the statistical software package R version 2.21 (R Development Core Team, 2005).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ole,Hartvig,Mortensen
| | Sample_contact_email | ole@hartvig.org
| | Sample_contact_laboratory | Cellular and Metabolic Research Section
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Copenhagen
| | Sample_contact_address | Panum 6.5.8, Blegdamsvej 3
| | Sample_contact_city | Copenhagen N
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM269nnn/GSM269834/suppl/GSM269834.CEL.gz
| | Sample_series_id | GSE10685
| | Sample_data_row_count | 54675
| | |
|
GSM269835 | GPL570 |
|
Subject DP, 6h
|
Human skeletal muscle biopsy from a 3h IL-6 infusion
|
Subject: DP
Timepoint: 6h
Sex: Male
|
Human skeletal muscle biopsy from vastus lateralis of musculus quadriceps muscle from a male subject.
|
| Sample_geo_accession | GSM269835
| | Sample_status | Public on May 28 2008
| | Sample_submission_date | Feb 29 2008
| | Sample_last_update_date | May 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | IL-6 infusion from 0h to 3h
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol, followed by RNEasy (Qiagen) purification of total RNA
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was synthesized to double-strand cDNA using Superscript Choice System (Invitrogen, Carlsbad, CA, USA) with an oligo-dT primer containing a T7 RNA polymerase promoter (GenSet, Evry, France). The cDNA was used as a template for in vitro transcription reaction to synthesize biotin-labeled antisense cRNA (BioArray High Yield RNA Transcript Labeling Kit; Enzo Diagnostics, Farmingdale, NY, USA)
| | Sample_hyb_protocol | After fragmentation at 94 C for 35 min in fragmentation buffer (40 mM Tris, 30 mM MgOAc, 10 mM KOAc), the labeled cRNA was hybridized for 16 h to Affymetrix HG-U133 Plus 2.0 (Affymetrix Inc., Santa Clara, CA, USA).After hybridization the array was washed and stained with streptavidin phycoerythrin solution using the Fluidics Station 400 (Affymetrix Inc.).
| | Sample_scan_protocol | Finally the arrays were scanned using the GeneChip Scanner 3000 (Affymetrix Inc.) to obtain non-normalized expression levels.
| | Sample_data_processing | Affymetrix CEL-files were normalized using gcRMA (Wu et al., 2004) available in the Bioconductor package version 1.7 (http://www.bioconductor.org) for the statistical software package R version 2.21 (R Development Core Team, 2005).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ole,Hartvig,Mortensen
| | Sample_contact_email | ole@hartvig.org
| | Sample_contact_laboratory | Cellular and Metabolic Research Section
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Copenhagen
| | Sample_contact_address | Panum 6.5.8, Blegdamsvej 3
| | Sample_contact_city | Copenhagen N
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM269nnn/GSM269835/suppl/GSM269835.CEL.gz
| | Sample_series_id | GSE10685
| | Sample_data_row_count | 54675
| | |
|
GSM269932 | GPL570 |
|
Subject HH, 0h
|
Human skeletal muscle biopsy from a 3h IL-6 infusion
|
Subject: HH
Timepoint: 0h
Sex: Male
|
Human skeletal muscle biopsy from vastus lateralis of musculus quadriceps muscle from a male subject.
|
| Sample_geo_accession | GSM269932
| | Sample_status | Public on May 28 2008
| | Sample_submission_date | Feb 29 2008
| | Sample_last_update_date | May 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | IL-6 infusion from 0h to 3h
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol, followed by RNEasy (Qiagen) purification of total RNA
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was synthesized to double-strand cDNA using Superscript Choice System (Invitrogen, Carlsbad, CA, USA) with an oligo-dT primer containing a T7 RNA polymerase promoter (GenSet, Evry, France). The cDNA was used as a template for in vitro transcription reaction to synthesize biotin-labeled antisense cRNA (BioArray High Yield RNA Transcript Labeling Kit; Enzo Diagnostics, Farmingdale, NY, USA)
| | Sample_hyb_protocol | After fragmentation at 94 C for 35 min in fragmentation buffer (40 mM Tris, 30 mM MgOAc, 10 mM KOAc), the labeled cRNA was hybridized for 16 h to Affymetrix HG-U133 Plus 2.0 (Affymetrix Inc., Santa Clara, CA, USA).After hybridization the array was washed and stained with streptavidin phycoerythrin solution using the Fluidics Station 400 (Affymetrix Inc.).
| | Sample_scan_protocol | Finally the arrays were scanned using the GeneChip Scanner 3000 (Affymetrix Inc.) to obtain non-normalized expression levels.
| | Sample_data_processing | Affymetrix CEL-files were normalized using gcRMA (Wu et al., 2004) available in the Bioconductor package version 1.7 (http://www.bioconductor.org) for the statistical software package R version 2.21 (R Development Core Team, 2005).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ole,Hartvig,Mortensen
| | Sample_contact_email | ole@hartvig.org
| | Sample_contact_laboratory | Cellular and Metabolic Research Section
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Copenhagen
| | Sample_contact_address | Panum 6.5.8, Blegdamsvej 3
| | Sample_contact_city | Copenhagen N
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM269nnn/GSM269932/suppl/GSM269932.CEL.gz
| | Sample_series_id | GSE10685
| | Sample_data_row_count | 54675
| | |
|
GSM269933 | GPL570 |
|
Subject HH, 3h
|
Human skeletal muscle biopsy from a 3h IL-6 infusion
|
Subject: HH
Timepoint: 3h
Sex: Male
|
Human skeletal muscle biopsy from vastus lateralis of musculus quadriceps muscle from a male subject.
|
| Sample_geo_accession | GSM269933
| | Sample_status | Public on May 28 2008
| | Sample_submission_date | Feb 29 2008
| | Sample_last_update_date | May 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | IL-6 infusion from 0h to 3h
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol, followed by RNEasy (Qiagen) purification of total RNA
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was synthesized to double-strand cDNA using Superscript Choice System (Invitrogen, Carlsbad, CA, USA) with an oligo-dT primer containing a T7 RNA polymerase promoter (GenSet, Evry, France). The cDNA was used as a template for in vitro transcription reaction to synthesize biotin-labeled antisense cRNA (BioArray High Yield RNA Transcript Labeling Kit; Enzo Diagnostics, Farmingdale, NY, USA)
| | Sample_hyb_protocol | After fragmentation at 94 C for 35 min in fragmentation buffer (40 mM Tris, 30 mM MgOAc, 10 mM KOAc), the labeled cRNA was hybridized for 16 h to Affymetrix HG-U133 Plus 2.0 (Affymetrix Inc., Santa Clara, CA, USA).After hybridization the array was washed and stained with streptavidin phycoerythrin solution using the Fluidics Station 400 (Affymetrix Inc.).
| | Sample_scan_protocol | Finally the arrays were scanned using the GeneChip Scanner 3000 (Affymetrix Inc.) to obtain non-normalized expression levels.
| | Sample_data_processing | Affymetrix CEL-files were normalized using gcRMA (Wu et al., 2004) available in the Bioconductor package version 1.7 (http://www.bioconductor.org) for the statistical software package R version 2.21 (R Development Core Team, 2005).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ole,Hartvig,Mortensen
| | Sample_contact_email | ole@hartvig.org
| | Sample_contact_laboratory | Cellular and Metabolic Research Section
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Copenhagen
| | Sample_contact_address | Panum 6.5.8, Blegdamsvej 3
| | Sample_contact_city | Copenhagen N
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM269nnn/GSM269933/suppl/GSM269933.CEL.gz
| | Sample_series_id | GSE10685
| | Sample_data_row_count | 54675
| | |
|
GSM269934 | GPL570 |
|
Subject HH, 6h
|
Human skeletal muscle biopsy from a 3h IL-6 infusion
|
Subject: HH
Timepoint: 6h
Sex: Male
|
Human skeletal muscle biopsy from vastus lateralis of musculus quadriceps muscle from a male subject.
|
| Sample_geo_accession | GSM269934
| | Sample_status | Public on May 28 2008
| | Sample_submission_date | Feb 29 2008
| | Sample_last_update_date | May 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | IL-6 infusion from 0h to 3h
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol, followed by RNEasy (Qiagen) purification of total RNA
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA was synthesized to double-strand cDNA using Superscript Choice System (Invitrogen, Carlsbad, CA, USA) with an oligo-dT primer containing a T7 RNA polymerase promoter (GenSet, Evry, France). The cDNA was used as a template for in vitro transcription reaction to synthesize biotin-labeled antisense cRNA (BioArray High Yield RNA Transcript Labeling Kit; Enzo Diagnostics, Farmingdale, NY, USA)
| | Sample_hyb_protocol | After fragmentation at 94 C for 35 min in fragmentation buffer (40 mM Tris, 30 mM MgOAc, 10 mM KOAc), the labeled cRNA was hybridized for 16 h to Affymetrix HG-U133 Plus 2.0 (Affymetrix Inc., Santa Clara, CA, USA).After hybridization the array was washed and stained with streptavidin phycoerythrin solution using the Fluidics Station 400 (Affymetrix Inc.).
| | Sample_scan_protocol | Finally the arrays were scanned using the GeneChip Scanner 3000 (Affymetrix Inc.) to obtain non-normalized expression levels.
| | Sample_data_processing | Affymetrix CEL-files were normalized using gcRMA (Wu et al., 2004) available in the Bioconductor package version 1.7 (http://www.bioconductor.org) for the statistical software package R version 2.21 (R Development Core Team, 2005).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ole,Hartvig,Mortensen
| | Sample_contact_email | ole@hartvig.org
| | Sample_contact_laboratory | Cellular and Metabolic Research Section
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Copenhagen
| | Sample_contact_address | Panum 6.5.8, Blegdamsvej 3
| | Sample_contact_city | Copenhagen N
| | Sample_contact_zip/postal_code | 2200
| | Sample_contact_country | Denmark
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM269nnn/GSM269934/suppl/GSM269934.CEL.gz
| | Sample_series_id | GSE10685
| | Sample_data_row_count | 54675
| | |
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