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Search results for the GEO ID: GSE10741
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GSM271153
GPL1261
BMDC control cells Bone marrow-derived dendritic cells, not 1D8 treated C57BL/6 mice, Gender: Male, Age: 6~8 weeks. BMDCs were prepared from bone marrow cells collected by removing the femur bones, cutting off each end and flushing out the bone marrow with RPMI 1640 medium using a syringe. The pooled cells were harvested by centrifugation at 600 g, 10 min and resuspended in 2 ml ACK buffer for 5 min at room temperature to lyse the red blood cells. These cells were washed in RPMI-1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin and the Dendritic Cells were cultured in medium containing 500 U/ml GM-CSF (R&D systems, MN, USA) for 7days. Hybridization was performed by the Johns Hopkins Medical Institutions Microarray Core Facility.
GSM271154
GPL1261
BMDCs cocultured with irradiated tumor Bone marrow-derived dendritic cells, irradiated 1D8 treated C57BL/6 mice, Gender: Male, Age: 6~8 weeks. BMDCs were prepared from bone marrow cells collected by removing the femur bones, cutting off each end and flushing out the bone marrow with RPMI 1640 medium using a syringe. The pooled cells were harvested by centrifugation at 600 g, 10 min and resuspended in 2 ml ACK buffer for 5 min at room temperature to lyse the red blood cells. These cells were washed in RPMI-1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin and the Dendritic Cells were cultured in medium containing 500 U/ml GM-CSF (R&D systems, MN, USA) for 7days. They were cocultured with irradiated (50Gy) 1D8 mouse ovarian surface epithelial tumor cells at a ratio 1:5, tumor:BMDC Hybridization was performed by the Johns Hopkins Medical Institutions Microarray Core Facility.
GSM271155
GPL1261
BDMCs cultured in tumor-conditioned medium Bone marrow-derived dendritic cells, 1D8 tumor cell supernatent treated C57BL/6 mice, Gender: Male, Age: 6~8 weeks. BMDCs were prepared from bone marrow cells collected by removing the femur bones, cutting off each end and flushing out the bone marrow with RPMI 1640 medium using a syringe. The pooled cells were harvested by centrifugation at 600 g, 10 min and resuspended in 2 ml ACK buffer for 5 min at room temperature to lyse the red blood cells. These cells were washed in RPMI-1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin and the Dendritic Cells were cultured in medium containing 500 U/ml GM-CSF (R&D systems, MN, USA) for 7days. They were cocultured in 1D8 (mouse ovarian surface epithelial cell) tumor cell-conditioned medium at 25% (v/v). Hybridization was performed by the Johns Hopkins Medical Institutions Microarray Core Facility.
GSM271156
GPL1261
BMDCs cocultured with tumor Bone marrow-derived dendritic cells, 1D8 treated C57BL/6 mice, Gender: Male, Age: 6~8 weeks. BMDCs were prepared from bone marrow cells collected by removing the femur bones, cutting off each end and flushing out the bone marrow with RPMI 1640 medium using a syringe. The pooled cells were harvested by centrifugation at 600 g, 10 min and resuspended in 2 ml ACK buffer for 5 min at room temperature to lyse the red blood cells. These cells were washed in RPMI-1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin and the Dendritic Cells were cultured in medium containing 500 U/ml GM-CSF (R&D systems, MN, USA) for 7days. They were cocultured with 1D8 mouse ovarian surface epithelial tumor cells at a ratio 1:5, tumor:BMDC Hybridization was performed by the Johns Hopkins Medical Institutions Microarray Core Facility.
 
 
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