Search results for the GEO ID: GSE10799  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM272755 | GPL570 |
|
Adenocarcinoma, bone marrow negative AC 3
|
Human pulmonary adenocarcinoma
|
male pulmonary adenocarcinoma
bone marrow negative
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272755
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272755/suppl/GSM272755.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272756 | GPL570 |
|
Adenocarcinoma, bone marrow negative AC 8
|
Human pulmonary adenocarcinoma
|
female pulmonary adenocarcinoma
bone marrow negative
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272756
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272756/suppl/GSM272756.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272757 | GPL570 |
|
Adenocarcinoma, bone marrow negative AC 10
|
Human pulmonary adenocarcinoma
|
female pulmonary adenocarcinoma
bone marrow negative
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272757
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272757/suppl/GSM272757.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272758 | GPL570 |
|
Adenocarcinoma, bone marrow negative AC 11
|
Human pulmonary adenocarcinoma
|
male pulmonary adenocarcinoma
bone marrow negative
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272758
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272758/suppl/GSM272758.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272759 | GPL570 |
|
Adenocarcinoma, bone marrow negative AC 12
|
Human pulmonary adenocarcinoma
|
female pulmonary adenocarcinoma
bone marrow negative
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272759
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272759/suppl/GSM272759.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272760 | GPL570 |
|
Adenocarcinoma, bone marrow negative AC 14
|
Human pulmonary adenocarcinoma
|
male pulmonary adenocarcinoma
bone marrow negative
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272760
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272760/suppl/GSM272760.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272761 | GPL570 |
|
Adenocarcinoma, bone marrow negative AC 15
|
Human pulmonary adenocarcinoma
|
female pulmonary adenocarcinoma
bone marrow negative
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272761
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272761/suppl/GSM272761.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272762 | GPL570 |
|
Adenocarcinoma, bone marrow negative AC 16
|
Human pulmonary adenocarcinoma
|
male pulmonary adenocarcinoma
bone marrow negative
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272762
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272762/suppl/GSM272762.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272763 | GPL570 |
|
Adenocarcinoma, bone marrow positive AC 22
|
Human pulmonary adenocarcinoma
|
female pulmonary adenocarcinoma
bone marrow positive
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272763
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272763/suppl/GSM272763.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272764 | GPL570 |
|
Adenocarcinoma, bone marrow positive AC 23
|
Human pulmonary adenocarcinoma
|
male pulmonary adenocarcinoma
bone marrow positive
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272764
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272764/suppl/GSM272764.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272765 | GPL570 |
|
Adenocarcinoma, bone marrow positive AC 25
|
Human pulmonary adenocarcinoma
|
male pulmonary adenocarcinoma
bone marrow positive
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272765
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272765/suppl/GSM272765.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272766 | GPL570 |
|
Adenocarcinoma, bone marrow positive AC 27
|
Human pulmonary adenocarcinoma
|
female pulmonary adenocarcinoma
bone marrow positive
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272766
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272766/suppl/GSM272766.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272767 | GPL570 |
|
Adenocarcinoma, bone marrow positive AC 30
|
Human pulmonary adenocarcinoma
|
male pulmonary adenocarcinoma
bone marrow positive
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272767
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272767/suppl/GSM272767.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272768 | GPL570 |
|
Adenocarcinoma, bone marrow positive AC 33
|
Human pulmonary adenocarcinoma
|
male pulmonary adenocarcinoma
bone marrow positive
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272768
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272768/suppl/GSM272768.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272769 | GPL570 |
|
Adenocarcinoma, bone marrow positive AC 34
|
Human pulmonary adenocarcinoma
|
male pulmonary adenocarcinoma
bone marrow positive
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272769
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272769/suppl/GSM272769.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272770 | GPL570 |
|
Normal N1
|
Human pulmonary lung
|
female pulmonary adenocarcinoma
normal tissue
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272770
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272770/suppl/GSM272770.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272771 | GPL570 |
|
Normal N2
|
Human pulmonary lung
|
female pulmonary adenocarcinoma
normal tissue
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272771
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272771/suppl/GSM272771.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
| | |
|
GSM272772 | GPL570 |
|
Normal N3
|
Human pulmonary lung
|
male pulmonary lung
normal tissue
|
Gene expression profile of lung tumor
|
| Sample_geo_accession | GSM272772
| | Sample_status | Public on Mar 11 2009
| | Sample_submission_date | Mar 12 2008
| | Sample_last_update_date | Mar 05 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | None
| | Sample_growth_protocol_ch1 | None
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Micro Kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50 ng total RNA
| | Sample_hyb_protocol | Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on hgu133plus2 Chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. Post Hyb Wash No.1: 10 cycles of of 2 mixes/cycle with Wash Buffer A at 25°C. Post Hyb Wash No.2: 4 cycles of 15 mixes/cycle with Wash Buffer B at 50°C. Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Post Stain Wash: 10 cycles of 4 mixes/cycle with Wash Buffer A at 25°C. 2nd Stain: Stain the probe array for 10 minutes in antibody solution at 25°C. 3rd Stain: Stain the probe array for 10 minutes in SAPE solution at 25°C. Final Wash: 15 cycles of 4 mixes/cycle with Wash Buffer A at 30°C. The holding temperature is 25°C. Hybridization Buffer: 100 mM MES 1 M [Na+] 20 mM EDTA 0.01% Tween 20. Wash Buffer A (Nonstringent): 6x SSPE 0.01% Tween 20. Wash Buffer B (Stringent): 100 mM MES 0.1 M [Na+] 0.01% Tween 20. Stain Buffer: 100 mM MES 1 M [Na+] 0.05% Tween 20. SAPE Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 10 μg/ml Streptavidin Phycoerythrin. Antibody Solution: 100 mM MES 1 M [Na+] 0.05% Tween 20 2 mg/ml acetylated BSA 0.1 mg/ml Normal Goat IgG 3 μg/ml biotinylated Anti-Streptavidin.
| | Sample_scan_protocol | Affymetrix GeneChip Scanner 7G Pixel Size: 1.56 μ Filter: 570 nm Number of Scans: 1 Affymetrix GeneChip Operating Software 1.3
| | Sample_data_processing | Data preprocessing using the GC Robust Multi-array Average (GC-RMA) method. Signal scaling against the median probe signal of three normal patients and log2-transformation. Genes for which information about the physical location is not given in the Affymetrix annotations files or is ambiguous are removed.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Thomas,,Streichert
| | Sample_contact_department | Institute For Clinical Chemistry / Central Laboratories
| | Sample_contact_institute | University Medical Center Hamburg-Eppendorf
| | Sample_contact_address | Martinistr. 52, Campus Forschung N27, 2. OG
| | Sample_contact_city | Hamburg
| | Sample_contact_zip/postal_code | D-20246
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272772/suppl/GSM272772.CEL.gz
| | Sample_series_id | GSE10799
| | Sample_data_row_count | 54675
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