Search results for the GEO ID: GSE10821  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM272994 | GPL570 |
|
GC B cell_pSG5_rep1
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GC B cell transfected with empty vector (pSG5), rep1
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GC B cells sorted using CD10 MACS from reactive human tonsils
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Amplified total RNA from transfected and FACS sorted GC Bells
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| Sample_geo_accession | GSM272994
| | Sample_status | Public on Oct 10 2008
| | Sample_submission_date | Mar 13 2008
| | Sample_last_update_date | Oct 10 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; Institute for Cancer Studies, Birmingham
| | Sample_treatment_protocol_ch1 | 4 x E6 CD10+ cells in 100 ul B cell solution were nucleofected with 7ug pSG5 or pSG5-LMP1 expression vector with 3ug pMACSLNFGR using program U15 on the Nucleofector I (Amaxa); cultivated for 16h and stained with anti-LNGRF-FITC or anti-LNGRF- Allophycocyanin and propidium iodide. FITC-labeled and PI-negative cells were collected by FACS on a MoFlo sorter
| | Sample_growth_protocol_ch1 | GC B cells were cultivated for 16 hours post-transfection in RPMI supplemented with 10% FCS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Micro Kit, Qiagen, and RNA was amplified with ExpressArt mRNA Amplification kit protocol (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix labelling protocol
| | Sample_hyb_protocol | Affymetrix hybridization protocol onto Human Genome U133 Plus 2.0 Array
| | Sample_scan_protocol | Affymetrix Standard protocol
| | Sample_data_processing | GCOS, RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM272nnn/GSM272994/suppl/GSM272994.CEL.gz
| | Sample_series_id | GSE10821
| | Sample_data_row_count | 54675
| | |
|
GSM273007 | GPL570 |
|
GC B cell_pSG5_rep2
|
GC B cell transfected with empty vector (pSG5), rep2
|
GC B cells sorted using CD10 MACS from reactive human tonsils
|
Amplified total RNA from transfected and FACS sorted GC Bells
|
| Sample_geo_accession | GSM273007
| | Sample_status | Public on Oct 10 2008
| | Sample_submission_date | Mar 13 2008
| | Sample_last_update_date | Oct 10 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; Institute for Cancer Studies, Birmingham
| | Sample_treatment_protocol_ch1 | 4 x E6 CD10+ cells in 100 ul B cell solution were nucleofected with 7ug pSG5 or pSG5-LMP1 expression vector with 3ug pMACSLNFGR using program U15 on the Nucleofector I (Amaxa); cultivated for 16h and stained with anti-LNGRF-FITC or anti-LNGRF- Allophycocyanin and propidium iodide. FITC-labeled and PI-negative cells were collected by FACS on a MoFlo sorter
| | Sample_growth_protocol_ch1 | GC B cells were cultivated for 16 hours post-transfection in RPMI supplemented with 10% FCS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Micro Kit, Qiagen, and RNA was amplified with ExpressArt mRNA Amplification kit protocol (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix labelling protocol
| | Sample_hyb_protocol | Affymetrix hybridization protocol onto Human Genome U133 Plus 2.0 Array
| | Sample_scan_protocol | Affymetrix Standard protocol
| | Sample_data_processing | GCOS, RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM273nnn/GSM273007/suppl/GSM273007.CEL.gz
| | Sample_series_id | GSE10821
| | Sample_data_row_count | 54675
| | |
|
GSM273008 | GPL570 |
|
GC B cell_pSG5_rep3
|
GC B cell transfected with empty vector (pSG5), rep3
|
GC B cells sorted using CD10 MACS from reactive human tonsils
|
Amplified total RNA from transfected and FACS sorted GC Bells
|
| Sample_geo_accession | GSM273008
| | Sample_status | Public on Oct 10 2008
| | Sample_submission_date | Mar 13 2008
| | Sample_last_update_date | Oct 10 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; Institute for Cancer Studies, Birmingham
| | Sample_treatment_protocol_ch1 | 4 x E6 CD10+ cells in 100 ul B cell solution were nucleofected with 7ug pSG5 or pSG5-LMP1 expression vector with 3ug pMACSLNFGR using program U15 on the Nucleofector I (Amaxa); cultivated for 16h and stained with anti-LNGRF-FITC or anti-LNGRF- Allophycocyanin and propidium iodide. FITC-labeled and PI-negative cells were collected by FACS on a MoFlo sorter
| | Sample_growth_protocol_ch1 | GC B cells were cultivated for 16 hours post-transfection in RPMI supplemented with 10% FCS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Micro Kit, Qiagen, and RNA was amplified with ExpressArt mRNA Amplification kit protocol (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix labelling protocol
| | Sample_hyb_protocol | Affymetrix hybridization protocol onto Human Genome U133 Plus 2.0 Array
| | Sample_scan_protocol | Affymetrix Standard protocol
| | Sample_data_processing | GCOS, RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM273nnn/GSM273008/suppl/GSM273008.CEL.gz
| | Sample_series_id | GSE10821
| | Sample_data_row_count | 54675
| | |
|
GSM273009 | GPL570 |
|
GC B cell_LMP1_rep1
|
GC B cell transfected with LMP1 expression vector (pSG5-LMP1), rep1
|
GC B cells sorted using CD10 MACS from reactive human tonsils
|
Amplified total RNA from transfected and FACS sorted GC Bells
|
| Sample_geo_accession | GSM273009
| | Sample_status | Public on Oct 10 2008
| | Sample_submission_date | Mar 13 2008
| | Sample_last_update_date | Oct 10 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; Institute for Cancer Studies, Birmingham
| | Sample_treatment_protocol_ch1 | 4 x E6 CD10+ cells in 100 ul B cell solution were nucleofected with 7ug pSG5 or pSG5-LMP1 expression vector with 3ug pMACSLNFGR using program U15 on the Nucleofector I (Amaxa); cultivated for 16h and stained with anti-LNGRF-FITC or anti-LNGRF- Allophycocyanin and propidium iodide. FITC-labeled and PI-negative cells were collected by FACS on a MoFlo sorter
| | Sample_growth_protocol_ch1 | GC B cells were cultivated for 16 hours post-transfection in RPMI supplemented with 10% FCS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Micro Kit, Qiagen, and RNA was amplified with ExpressArt mRNA Amplification kit protocol (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix labelling protocol
| | Sample_hyb_protocol | Affymetrix hybridization protocol onto Human Genome U133 Plus 2.0 Array
| | Sample_scan_protocol | Affymetrix Standard protocol
| | Sample_data_processing | GCOS, RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM273nnn/GSM273009/suppl/GSM273009.CEL.gz
| | Sample_series_id | GSE10821
| | Sample_data_row_count | 54675
| | |
|
GSM273012 | GPL570 |
|
GC B cell_LMP1_rep2
|
GC B cell transfected with LMP1 expression vector (pSG5-LMP1), rep2
|
GC B cells sorted using CD10 MACS from reactive human tonsils
|
Amplified total RNA from transfected and FACS sorted GC Bells
|
| Sample_geo_accession | GSM273012
| | Sample_status | Public on Oct 10 2008
| | Sample_submission_date | Mar 13 2008
| | Sample_last_update_date | Oct 10 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; Institute for Cancer Studies, Birmingham
| | Sample_treatment_protocol_ch1 | 4 x E6 CD10+ cells in 100 ul B cell solution were nucleofected with 7ug pSG5 or pSG5-LMP1 expression vector with 3ug pMACSLNFGR using program U15 on the Nucleofector I (Amaxa); cultivated for 16h and stained with anti-LNGRF-FITC or anti-LNGRF- Allophycocyanin and propidium iodide. FITC-labeled and PI-negative cells were collected by FACS on a MoFlo sorter
| | Sample_growth_protocol_ch1 | GC B cells were cultivated for 16 hours post-transfection in RPMI supplemented with 10% FCS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Micro Kit, Qiagen, and RNA was amplified with ExpressArt mRNA Amplification kit protocol (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix labelling protocol
| | Sample_hyb_protocol | Affymetrix hybridization protocol onto Human Genome U133 Plus 2.0 Array
| | Sample_scan_protocol | Affymetrix Standard protocol
| | Sample_data_processing | GCOS, RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM273nnn/GSM273012/suppl/GSM273012.CEL.gz
| | Sample_series_id | GSE10821
| | Sample_data_row_count | 54675
| | |
|
GSM273014 | GPL570 |
|
GC B cell_LMP1_rep3
|
GC B cell transfected with LMP1 expression vector (pSG5-LMP1), rep3
|
GC B cells sorted using CD10 MACS from reactive human tonsils
|
Amplified total RNA from transfected and FACS sorted GC Bells
|
| Sample_geo_accession | GSM273014
| | Sample_status | Public on Oct 10 2008
| | Sample_submission_date | Mar 13 2008
| | Sample_last_update_date | Oct 10 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; Institute for Cancer Studies, Birmingham
| | Sample_treatment_protocol_ch1 | 4 x E6 CD10+ cells in 100 ul B cell solution were nucleofected with 7ug pSG5 or pSG5-LMP1 expression vector with 3ug pMACSLNFGR using program U15 on the Nucleofector I (Amaxa); cultivated for 16h and stained with anti-LNGRF-FITC or anti-LNGRF- Allophycocyanin and propidium iodide. FITC-labeled and PI-negative cells were collected by FACS on a MoFlo sorter
| | Sample_growth_protocol_ch1 | GC B cells were cultivated for 16 hours post-transfection in RPMI supplemented with 10% FCS and antibiotics.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Micro Kit, Qiagen, and RNA was amplified with ExpressArt mRNA Amplification kit protocol (Amptech).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix labelling protocol
| | Sample_hyb_protocol | Affymetrix hybridization protocol onto Human Genome U133 Plus 2.0 Array
| | Sample_scan_protocol | Affymetrix Standard protocol
| | Sample_data_processing | GCOS, RMA
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wenbin,,Wei
| | Sample_contact_email | w.wei@bham.ac.uk
| | Sample_contact_phone | +44-121-4143293
| | Sample_contact_laboratory | Bioinformatics
| | Sample_contact_department | School of Cancer Sciences
| | Sample_contact_institute | University of Birmingham
| | Sample_contact_address | Vincent Drive
| | Sample_contact_city | Edgbaston
| | Sample_contact_state | West Midlands
| | Sample_contact_zip/postal_code | B15 2TT
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM273nnn/GSM273014/suppl/GSM273014.CEL.gz
| | Sample_series_id | GSE10821
| | Sample_data_row_count | 54675
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