Result

Search results for the GEO ID: GSE10832

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM274607

GPL570

3PPS07042001_PC3_cell_monolayer

PC3_cell_monolayer

Sample_geo_accession	GSM274607
Sample_status	Public on Dec 09 2008
Sample_submission_date	Mar 14 2008
Sample_last_update_date	Dec 09 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	For flow cytometry, cell were dissociated with Accutase (Innovative Cell Tech Inc., San Diego, CA, USA) and washed 2 times in the staining solution containing Ca2+ and Mg2+-free PBS with 1mM ethylenediaminetetraacetic acid (EDTA), 25mM Hepes (pH 7.0) (Gibco BRL), 0.5% FBS. Cells were stained live in the staining solution containing conjugated anti-CD44 and anti-CD133 antibody (50 min at 4oC). Samples were analyzed on BD LSR II flow cytometer (Beckton Dickinson Immunocytometry Systems, San Jose, CA). A minimum of (or At least) 500,000 viable cells were measured per sample. For FACS, 2x107 cells were processed for double CD44 and CD133 staining along with appropriate negative controls and single color positive controls. The CD44+/CD133+ and CD44-/CD133- populations were sorted out on a BD FACS Diva cell sorter (Beckton Dickinson Immunocytometry Systems, San Jose, CA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from cell pellets using the RNeasy kit (Qiagen, Valencia, CA, USA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	50 ng total RNA was used in a standard Affymetrix double amplification protocol.
Sample_hyb_protocol	standard Affymetrix procedures
Sample_scan_protocol	standard Affymetrix procedures
Sample_data_processing	gcRMA, scaled to a median intensity of 100
Sample_platform_id	GPL570
Sample_contact_name	John,R,Walker
Sample_contact_email	jwalker@gnf.org
Sample_contact_phone	858-812-1636
Sample_contact_laboratory	Genetics Core
Sample_contact_department
Sample_contact_institute	Genomics Institute of the Novartis Research Foundation
Sample_contact_address	10675 John Jay Hopkins
Sample_contact_city	San Diego
Sample_contact_state	CA
Sample_contact_zip/postal_code	92121
Sample_contact_country	USA
Sample_contact_web_link	http://biogps.gnf.org
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM274nnn/GSM274607/suppl/GSM274607.CEL.gz
Sample_series_id	GSE10832
Sample_data_row_count	54675

GSM274608

GPL570

3PPS07042002_PC3_cell_spheres

PC3_cell_spheres

Sample_geo_accession	GSM274608
Sample_status	Public on Dec 09 2008
Sample_submission_date	Mar 14 2008
Sample_last_update_date	Dec 09 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	For flow cytometry, cell were dissociated with Accutase (Innovative Cell Tech Inc., San Diego, CA, USA) and washed 2 times in the staining solution containing Ca2+ and Mg2+-free PBS with 1mM ethylenediaminetetraacetic acid (EDTA), 25mM Hepes (pH 7.0) (Gibco BRL), 0.5% FBS. Cells were stained live in the staining solution containing conjugated anti-CD44 and anti-CD133 antibody (50 min at 4oC). Samples were analyzed on BD LSR II flow cytometer (Beckton Dickinson Immunocytometry Systems, San Jose, CA). A minimum of (or At least) 500,000 viable cells were measured per sample. For FACS, 2x107 cells were processed for double CD44 and CD133 staining along with appropriate negative controls and single color positive controls. The CD44+/CD133+ and CD44-/CD133- populations were sorted out on a BD FACS Diva cell sorter (Beckton Dickinson Immunocytometry Systems, San Jose, CA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from cell pellets using the RNeasy kit (Qiagen, Valencia, CA, USA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	50 ng total RNA was used in a standard Affymetrix double amplification protocol.
Sample_hyb_protocol	standard Affymetrix procedures
Sample_scan_protocol	standard Affymetrix procedures
Sample_data_processing	gcRMA, scaled to a median intensity of 100
Sample_platform_id	GPL570
Sample_contact_name	John,R,Walker
Sample_contact_email	jwalker@gnf.org
Sample_contact_phone	858-812-1636
Sample_contact_laboratory	Genetics Core
Sample_contact_department
Sample_contact_institute	Genomics Institute of the Novartis Research Foundation
Sample_contact_address	10675 John Jay Hopkins
Sample_contact_city	San Diego
Sample_contact_state	CA
Sample_contact_zip/postal_code	92121
Sample_contact_country	USA
Sample_contact_web_link	http://biogps.gnf.org
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM274nnn/GSM274608/suppl/GSM274608.CEL.gz
Sample_series_id	GSE10832
Sample_data_row_count	54675

GSM274609

GPL570

3PPS07042003_DU145_cell_monolayer

DU145_cell_monolayer

Sample_geo_accession	GSM274609
Sample_status	Public on Dec 09 2008
Sample_submission_date	Mar 14 2008
Sample_last_update_date	Dec 09 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	For flow cytometry, cell were dissociated with Accutase (Innovative Cell Tech Inc., San Diego, CA, USA) and washed 2 times in the staining solution containing Ca2+ and Mg2+-free PBS with 1mM ethylenediaminetetraacetic acid (EDTA), 25mM Hepes (pH 7.0) (Gibco BRL), 0.5% FBS. Cells were stained live in the staining solution containing conjugated anti-CD44 and anti-CD133 antibody (50 min at 4oC). Samples were analyzed on BD LSR II flow cytometer (Beckton Dickinson Immunocytometry Systems, San Jose, CA). A minimum of (or At least) 500,000 viable cells were measured per sample. For FACS, 2x107 cells were processed for double CD44 and CD133 staining along with appropriate negative controls and single color positive controls. The CD44+/CD133+ and CD44-/CD133- populations were sorted out on a BD FACS Diva cell sorter (Beckton Dickinson Immunocytometry Systems, San Jose, CA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from cell pellets using the RNeasy kit (Qiagen, Valencia, CA, USA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	50 ng total RNA was used in a standard Affymetrix double amplification protocol.
Sample_hyb_protocol	standard Affymetrix procedures
Sample_scan_protocol	standard Affymetrix procedures
Sample_data_processing	gcRMA, scaled to a median intensity of 100
Sample_platform_id	GPL570
Sample_contact_name	John,R,Walker
Sample_contact_email	jwalker@gnf.org
Sample_contact_phone	858-812-1636
Sample_contact_laboratory	Genetics Core
Sample_contact_department
Sample_contact_institute	Genomics Institute of the Novartis Research Foundation
Sample_contact_address	10675 John Jay Hopkins
Sample_contact_city	San Diego
Sample_contact_state	CA
Sample_contact_zip/postal_code	92121
Sample_contact_country	USA
Sample_contact_web_link	http://biogps.gnf.org
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM274nnn/GSM274609/suppl/GSM274609.CEL.gz
Sample_series_id	GSE10832
Sample_data_row_count	54675

GSM274610

GPL570

3PPS07042004_DU145_cell_spheres

DU145_cell_spheres

Sample_geo_accession	GSM274610
Sample_status	Public on Dec 09 2008
Sample_submission_date	Mar 14 2008
Sample_last_update_date	Dec 09 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	For flow cytometry, cell were dissociated with Accutase (Innovative Cell Tech Inc., San Diego, CA, USA) and washed 2 times in the staining solution containing Ca2+ and Mg2+-free PBS with 1mM ethylenediaminetetraacetic acid (EDTA), 25mM Hepes (pH 7.0) (Gibco BRL), 0.5% FBS. Cells were stained live in the staining solution containing conjugated anti-CD44 and anti-CD133 antibody (50 min at 4oC). Samples were analyzed on BD LSR II flow cytometer (Beckton Dickinson Immunocytometry Systems, San Jose, CA). A minimum of (or At least) 500,000 viable cells were measured per sample. For FACS, 2x107 cells were processed for double CD44 and CD133 staining along with appropriate negative controls and single color positive controls. The CD44+/CD133+ and CD44-/CD133- populations were sorted out on a BD FACS Diva cell sorter (Beckton Dickinson Immunocytometry Systems, San Jose, CA).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from cell pellets using the RNeasy kit (Qiagen, Valencia, CA, USA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	50 ng total RNA was used in a standard Affymetrix double amplification protocol.
Sample_hyb_protocol	standard Affymetrix procedures
Sample_scan_protocol	standard Affymetrix procedures
Sample_data_processing	gcRMA, scaled to a median intensity of 100
Sample_platform_id	GPL570
Sample_contact_name	John,R,Walker
Sample_contact_email	jwalker@gnf.org
Sample_contact_phone	858-812-1636
Sample_contact_laboratory	Genetics Core
Sample_contact_department
Sample_contact_institute	Genomics Institute of the Novartis Research Foundation
Sample_contact_address	10675 John Jay Hopkins
Sample_contact_city	San Diego
Sample_contact_state	CA
Sample_contact_zip/postal_code	92121
Sample_contact_country	USA
Sample_contact_web_link	http://biogps.gnf.org
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM274nnn/GSM274610/suppl/GSM274610.CEL.gz
Sample_series_id	GSE10832
Sample_data_row_count	54675

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