Search results for the GEO ID: GSE10879  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM275676 | GPL570 |
|
Wild-type MCF-7 cells, biological rep 1
|
Epithelial breast cancer cells
|
Estrogen-receptor positive, estrogen dependent and antihormone responsive
|
Gene expression data for wild-type MCF-7 cells grown in estrogen-free medium
|
| Sample_geo_accession | GSM275676
| | Sample_status | Public on Mar 19 2008
| | Sample_submission_date | Mar 18 2008
| | Sample_last_update_date | Mar 18 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were not treated with any drugs.
| | Sample_growth_protocol_ch1 | wild-type MCF-7 cells and estrogen-deprived MCF-7:5C, and MCF-7:2A cells were grown in estrogen-free media until they were 70-80% confluent and then RNA was extracted, labeled, and hybridized.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dr Joan,,Lewis-Wambi
| | Sample_contact_email | joan.lewis@fccc.edu
| | Sample_contact_phone | 215-728-4094
| | Sample_contact_fax | 215-728-7034
| | Sample_contact_department | Medical Sciences
| | Sample_contact_institute | Fox Chase Cancer Center
| | Sample_contact_address | 333 Cottman Ave
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19111
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275676/suppl/GSM275676.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275676/suppl/GSM275676.CHP.gz
| | Sample_series_id | GSE10879
| | Sample_data_row_count | 54675
| | |
|
GSM275677 | GPL570 |
|
Wild-type MCF-7 cells, biological rep 2
|
Epithelial breast cancer cells
|
Estrogen-receptor positive, estrogen dependent and antihormone responsive
|
Gene expression data for wild-type MCF-7 cells grown in estrogen-free medium
|
| Sample_geo_accession | GSM275677
| | Sample_status | Public on Mar 19 2008
| | Sample_submission_date | Mar 18 2008
| | Sample_last_update_date | Mar 18 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were not treated with any drugs.
| | Sample_growth_protocol_ch1 | wild-type MCF-7 cells and estrogen-deprived MCF-7:5C, and MCF-7:2A cells were grown in estrogen-free media until they were 70-80% confluent and then RNA was extracted, labeled, and hybridized.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dr Joan,,Lewis-Wambi
| | Sample_contact_email | joan.lewis@fccc.edu
| | Sample_contact_phone | 215-728-4094
| | Sample_contact_fax | 215-728-7034
| | Sample_contact_department | Medical Sciences
| | Sample_contact_institute | Fox Chase Cancer Center
| | Sample_contact_address | 333 Cottman Ave
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19111
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275677/suppl/GSM275677.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275677/suppl/GSM275677.CHP.gz
| | Sample_series_id | GSE10879
| | Sample_data_row_count | 54675
| | |
|
GSM275678 | GPL570 |
|
Wild-type MCF-7 cells, biological rep 3
|
Epithelial breast cancer cells
|
Estrogen-receptor positive, estrogen dependent and antihormone responsive
|
Gene expression data for wild-type MCF-7 cells grown in estrogen-free medium
|
| Sample_geo_accession | GSM275678
| | Sample_status | Public on Mar 19 2008
| | Sample_submission_date | Mar 18 2008
| | Sample_last_update_date | Mar 18 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were not treated with any drugs.
| | Sample_growth_protocol_ch1 | wild-type MCF-7 cells and estrogen-deprived MCF-7:5C, and MCF-7:2A cells were grown in estrogen-free media until they were 70-80% confluent and then RNA was extracted, labeled, and hybridized.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dr Joan,,Lewis-Wambi
| | Sample_contact_email | joan.lewis@fccc.edu
| | Sample_contact_phone | 215-728-4094
| | Sample_contact_fax | 215-728-7034
| | Sample_contact_department | Medical Sciences
| | Sample_contact_institute | Fox Chase Cancer Center
| | Sample_contact_address | 333 Cottman Ave
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19111
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275678/suppl/GSM275678.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275678/suppl/GSM275678.CHP.gz
| | Sample_series_id | GSE10879
| | Sample_data_row_count | 54675
| | |
|
GSM275679 | GPL570 |
|
Wild-type MCF-7 cells, biological rep 4
|
Epithelial breast cancer cells
|
Estrogen-receptor positive, estrogen dependent and antihormone responsive
|
Gene expression data for wild-type MCF-7 cells grown in estrogen-free medium
|
| Sample_geo_accession | GSM275679
| | Sample_status | Public on Mar 19 2008
| | Sample_submission_date | Mar 18 2008
| | Sample_last_update_date | Mar 18 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were not treated with any drugs.
| | Sample_growth_protocol_ch1 | wild-type MCF-7 cells and estrogen-deprived MCF-7:5C, and MCF-7:2A cells were grown in estrogen-free media until they were 70-80% confluent and then RNA was extracted, labeled, and hybridized.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dr Joan,,Lewis-Wambi
| | Sample_contact_email | joan.lewis@fccc.edu
| | Sample_contact_phone | 215-728-4094
| | Sample_contact_fax | 215-728-7034
| | Sample_contact_department | Medical Sciences
| | Sample_contact_institute | Fox Chase Cancer Center
| | Sample_contact_address | 333 Cottman Ave
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19111
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275679/suppl/GSM275679.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275679/suppl/GSM275679.CHP.gz
| | Sample_series_id | GSE10879
| | Sample_data_row_count | 54675
| | |
|
GSM275680 | GPL570 |
|
Estrogen-deprived MCF-7:5C cells, biological rep 1
|
Epithelial breast cancer cells
|
Estrogen-receptor positive, estrogen deprived, hormone-independent
|
Gene expression data for estrogen deprived MCF-7:5C cells grown in estrogen-free medium
|
| Sample_geo_accession | GSM275680
| | Sample_status | Public on Mar 19 2008
| | Sample_submission_date | Mar 18 2008
| | Sample_last_update_date | Mar 18 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were not treated with any drugs.
| | Sample_growth_protocol_ch1 | wild-type MCF-7 cells and estrogen-deprived MCF-7:5C, and MCF-7:2A cells were grown in estrogen-free media until they were 70-80% confluent and then RNA was extracted, labeled, and hybridized.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dr Joan,,Lewis-Wambi
| | Sample_contact_email | joan.lewis@fccc.edu
| | Sample_contact_phone | 215-728-4094
| | Sample_contact_fax | 215-728-7034
| | Sample_contact_department | Medical Sciences
| | Sample_contact_institute | Fox Chase Cancer Center
| | Sample_contact_address | 333 Cottman Ave
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19111
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275680/suppl/GSM275680.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275680/suppl/GSM275680.CHP.gz
| | Sample_series_id | GSE10879
| | Sample_data_row_count | 54675
| | |
|
GSM275681 | GPL570 |
|
Estrogen-deprived MCF-7:5C cells, biological rep 2
|
Epithelial breast cancer cells
|
Estrogen-receptor positive, estrogen deprived, hormone-independent
|
Gene expression data for estrogen deprived MCF-7:5C cells grown in estrogen-free medium
|
| Sample_geo_accession | GSM275681
| | Sample_status | Public on Mar 19 2008
| | Sample_submission_date | Mar 18 2008
| | Sample_last_update_date | Mar 18 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were not treated with any drugs.
| | Sample_growth_protocol_ch1 | wild-type MCF-7 cells and estrogen-deprived MCF-7:5C, and MCF-7:2A cells were grown in estrogen-free media until they were 70-80% confluent and then RNA was extracted, labeled, and hybridized.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dr Joan,,Lewis-Wambi
| | Sample_contact_email | joan.lewis@fccc.edu
| | Sample_contact_phone | 215-728-4094
| | Sample_contact_fax | 215-728-7034
| | Sample_contact_department | Medical Sciences
| | Sample_contact_institute | Fox Chase Cancer Center
| | Sample_contact_address | 333 Cottman Ave
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19111
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275681/suppl/GSM275681.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275681/suppl/GSM275681.CHP.gz
| | Sample_series_id | GSE10879
| | Sample_data_row_count | 54675
| | |
|
GSM275682 | GPL570 |
|
Estrogen-deprived MCF-7:5C cells, biological rep 3
|
Epithelial breast cancer cells
|
Estrogen-receptor positive, estrogen deprived, hormone-independent
|
Gene expression data for estrogen deprived MCF-7:5C cells grown in estrogen-free medium
|
| Sample_geo_accession | GSM275682
| | Sample_status | Public on Mar 19 2008
| | Sample_submission_date | Mar 18 2008
| | Sample_last_update_date | Mar 18 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were not treated with any drugs.
| | Sample_growth_protocol_ch1 | wild-type MCF-7 cells and estrogen-deprived MCF-7:5C, and MCF-7:2A cells were grown in estrogen-free media until they were 70-80% confluent and then RNA was extracted, labeled, and hybridized.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dr Joan,,Lewis-Wambi
| | Sample_contact_email | joan.lewis@fccc.edu
| | Sample_contact_phone | 215-728-4094
| | Sample_contact_fax | 215-728-7034
| | Sample_contact_department | Medical Sciences
| | Sample_contact_institute | Fox Chase Cancer Center
| | Sample_contact_address | 333 Cottman Ave
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19111
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275682/suppl/GSM275682.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275682/suppl/GSM275682.CHP.gz
| | Sample_series_id | GSE10879
| | Sample_data_row_count | 54675
| | |
|
GSM275683 | GPL570 |
|
Estrogen-deprived MCF-7:2A cells, biological rep 1
|
Epithelial breast cancer cells
|
Estrogen-receptor positive, estrogen deprived, hormone-independent
|
Gene expression data for estrogen deprived MCF-7:2A cells grown in estrogen-free medium
|
| Sample_geo_accession | GSM275683
| | Sample_status | Public on Mar 19 2008
| | Sample_submission_date | Mar 18 2008
| | Sample_last_update_date | Mar 18 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were not treated with any drugs.
| | Sample_growth_protocol_ch1 | wild-type MCF-7 cells and estrogen-deprived MCF-7:5C, and MCF-7:2A cells were grown in estrogen-free media until they were 70-80% confluent and then RNA was extracted, labeled, and hybridized.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dr Joan,,Lewis-Wambi
| | Sample_contact_email | joan.lewis@fccc.edu
| | Sample_contact_phone | 215-728-4094
| | Sample_contact_fax | 215-728-7034
| | Sample_contact_department | Medical Sciences
| | Sample_contact_institute | Fox Chase Cancer Center
| | Sample_contact_address | 333 Cottman Ave
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19111
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275683/suppl/GSM275683.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275683/suppl/GSM275683.CHP.gz
| | Sample_series_id | GSE10879
| | Sample_data_row_count | 54675
| | |
|
GSM275684 | GPL570 |
|
Estrogen-deprived MCF-7:2A cells, biological rep 2
|
Epithelial breast cancer cells
|
Estrogen-receptor positive, estrogen deprived, hormone-independent
|
Gene expression data for estrogen deprived MCF-7:2A cells grown in estrogen-free medium
|
| Sample_geo_accession | GSM275684
| | Sample_status | Public on Mar 19 2008
| | Sample_submission_date | Mar 18 2008
| | Sample_last_update_date | Mar 18 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were not treated with any drugs.
| | Sample_growth_protocol_ch1 | wild-type MCF-7 cells and estrogen-deprived MCF-7:5C, and MCF-7:2A cells were grown in estrogen-free media until they were 70-80% confluent and then RNA was extracted, labeled, and hybridized.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dr Joan,,Lewis-Wambi
| | Sample_contact_email | joan.lewis@fccc.edu
| | Sample_contact_phone | 215-728-4094
| | Sample_contact_fax | 215-728-7034
| | Sample_contact_department | Medical Sciences
| | Sample_contact_institute | Fox Chase Cancer Center
| | Sample_contact_address | 333 Cottman Ave
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19111
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275684/suppl/GSM275684.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275684/suppl/GSM275684.CHP.gz
| | Sample_series_id | GSE10879
| | Sample_data_row_count | 54675
| | |
|
GSM275685 | GPL570 |
|
Estrogen-deprived MCF-7:2A cells, biological rep 3
|
Epithelial breast cancer cells
|
Estrogen-receptor positive, estrogen deprived, hormone-independent
|
Gene expression data for estrogen deprived MCF-7:2A cells grown in estrogen-free medium
|
| Sample_geo_accession | GSM275685
| | Sample_status | Public on Mar 19 2008
| | Sample_submission_date | Mar 18 2008
| | Sample_last_update_date | Mar 18 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were not treated with any drugs.
| | Sample_growth_protocol_ch1 | wild-type MCF-7 cells and estrogen-deprived MCF-7:5C, and MCF-7:2A cells were grown in estrogen-free media until they were 70-80% confluent and then RNA was extracted, labeled, and hybridized.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dr Joan,,Lewis-Wambi
| | Sample_contact_email | joan.lewis@fccc.edu
| | Sample_contact_phone | 215-728-4094
| | Sample_contact_fax | 215-728-7034
| | Sample_contact_department | Medical Sciences
| | Sample_contact_institute | Fox Chase Cancer Center
| | Sample_contact_address | 333 Cottman Ave
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19111
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275685/suppl/GSM275685.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275685/suppl/GSM275685.CHP.gz
| | Sample_series_id | GSE10879
| | Sample_data_row_count | 54675
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GSM275686 | GPL570 |
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Estrogen-deprived MCF-7:2A cells, biological rep 4
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Epithelial breast cancer cells
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Estrogen-receptor positive, estrogen deprived, hormone-independent
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Gene expression data for estrogen deprived MCF-7:2A cells grown in estrogen-free medium
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| Sample_geo_accession | GSM275686
| | Sample_status | Public on Mar 19 2008
| | Sample_submission_date | Mar 18 2008
| | Sample_last_update_date | Mar 18 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were not treated with any drugs.
| | Sample_growth_protocol_ch1 | wild-type MCF-7 cells and estrogen-deprived MCF-7:5C, and MCF-7:2A cells were grown in estrogen-free media until they were 70-80% confluent and then RNA was extracted, labeled, and hybridized.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dr Joan,,Lewis-Wambi
| | Sample_contact_email | joan.lewis@fccc.edu
| | Sample_contact_phone | 215-728-4094
| | Sample_contact_fax | 215-728-7034
| | Sample_contact_department | Medical Sciences
| | Sample_contact_institute | Fox Chase Cancer Center
| | Sample_contact_address | 333 Cottman Ave
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19111
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275686/suppl/GSM275686.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM275nnn/GSM275686/suppl/GSM275686.CHP.gz
| | Sample_series_id | GSE10879
| | Sample_data_row_count | 54675
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