Search results for the GEO ID: GSE10896  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM276209 | GPL570 |
|
U937_naive_untreated_4h_rep1
|
differentiated U937 cells, untreated, 4h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276209
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276209/suppl/GSM276209.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276210 | GPL570 |
|
U937_naive_curcumin_4h_rep1
|
differentiated U937 cells, 1uM curcumin treated, 4h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276210
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276210/suppl/GSM276210.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276211 | GPL570 |
|
U937_ROS_untreated_4h_rep1
|
differentiated U937 cells, ROS exposed, 4h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276211
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276211/suppl/GSM276211.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276212 | GPL570 |
|
U937_ROS_curcumin_4h_rep1
|
differentiated U937 cells, ROS exposed, 1uM curcumin treated, 4h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276212
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276212/suppl/GSM276212.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276213 | GPL570 |
|
U937_naive_untreated_18h_rep1
|
differentiated U937 cells, untreated, 18h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276213
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276213/suppl/GSM276213.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276214 | GPL570 |
|
U937_naive_curcumin_18h_rep1
|
differentiated U937 cells, 1uM curcumin treated, 18h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276214
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276214/suppl/GSM276214.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276215 | GPL570 |
|
U937_ROS_untreated_18h_rep1
|
differentiated U937 cells, ROS exposed, 18h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276215
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276215/suppl/GSM276215.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276216 | GPL570 |
|
U937_ROS_curcumin_18h_rep1
|
differentiated U937 cells, ROS exposed, 1uM curcumin treated, 18h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276216
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276216/suppl/GSM276216.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276217 | GPL570 |
|
U937_naive_untreated_4h_rep2
|
differentiated U937 cells, untreated, 4h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276217
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276217/suppl/GSM276217.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276218 | GPL570 |
|
U937_naive_curcumin_4h_rep2
|
differentiated U937 cells, 1uM curcumin treated, 4h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276218
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276218/suppl/GSM276218.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276219 | GPL570 |
|
U937_ROS_untreated_4h_rep2
|
differentiated U937 cells, ROS exposed, 4h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276219
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276219/suppl/GSM276219.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276220 | GPL570 |
|
U937_ROS_curcumin_4h_rep2
|
differentiated U937 cells, ROS exposed, 1uM curcumin treated, 4h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276220
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276220/suppl/GSM276220.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276221 | GPL570 |
|
U937_naive_untreated_18h_rep2
|
differentiated U937 cells, untreated, 18h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276221
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276221/suppl/GSM276221.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276222 | GPL570 |
|
U937_naive_curcumin_18h_rep2
|
differentiated U937 cells, 1uM curcumin treated, 18h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276222
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276222/suppl/GSM276222.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276223 | GPL570 |
|
U937_ROS_untreated_18h_rep2
|
differentiated U937 cells, ROS exposed, 18h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276223
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276223/suppl/GSM276223.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276224 | GPL570 |
|
U937_ROS_curcumin_18h_rep2
|
differentiated U937 cells, ROS exposed, 1uM curcumin treated, 18h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276224
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276224/suppl/GSM276224.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276225 | GPL570 |
|
U937_naive_untreated_4h_rep3
|
differentiated U937 cells, untreated, 4h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276225
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276225/suppl/GSM276225.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276226 | GPL570 |
|
U937_naive_curcumin_4h_rep3
|
differentiated U937 cells, 1uM curcumin treated, 4h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276226
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276226/suppl/GSM276226.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276227 | GPL570 |
|
U937_ROS_untreated_4h_rep3
|
differentiated U937 cells, ROS exposed, 4h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276227
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276227/suppl/GSM276227.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276228 | GPL570 |
|
U937_ROS_curcumin_4h_rep3
|
differentiated U937 cells, ROS exposed, 1uM curcumin treated, 4h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276228
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276228/suppl/GSM276228.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276229 | GPL570 |
|
U937_naive_untreated_18h_rep3
|
differentiated U937 cells, untreated, 18h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276229
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276229/suppl/GSM276229.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276230 | GPL570 |
|
U937_naive_curcumin_18h_rep3
|
differentiated U937 cells, 1uM curcumin treated, 18h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276230
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276230/suppl/GSM276230.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276231 | GPL570 |
|
U937_ROS_untreated_18h_rep3
|
differentiated U937 cells, ROS exposed, 18h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276231
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276231/suppl/GSM276231.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
|
GSM276232 | GPL570 |
|
U937_ROS_curcumin_18h_rep3
|
differentiated U937 cells, ROS exposed, 1uM curcumin treated, 18h
|
Immortilised monocyte cell line
|
no additional information
|
| Sample_geo_accession | GSM276232
| | Sample_status | Public on Mar 20 2008
| | Sample_submission_date | Mar 19 2008
| | Sample_last_update_date | Mar 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
| | Sample_growth_protocol_ch1 | The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Paul,,Kirkham
| | Sample_contact_email | paul.kirkham@novartis.com
| | Sample_contact_institute | Novartis Institutes for Biomedical Research
| | Sample_contact_address | Wimblehurst Road
| | Sample_contact_city | Horsham
| | Sample_contact_state | west Sussex
| | Sample_contact_zip/postal_code | RH12 5AB
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276232/suppl/GSM276232.cel.gz
| | Sample_series_id | GSE10896
| | Sample_data_row_count | 54675
| | |
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