Search results for the GEO ID: GSE10904  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM276433 | GPL1261 |
|
Mutant, biological rep1
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Alb67 mutant mouse
|
Genotype: B6.Alb/cre,Pdss2loxP/loxP(Liver-conditional knock-out)
Age: 176-day-old male
70 mg liver used for RNA extraction
|
Gene expression data from mutant M. musculus
|
| Sample_geo_accession | GSM276433
| | Sample_status | Public on Apr 30 2008
| | Sample_submission_date | Mar 20 2008
| | Sample_last_update_date | Mar 20 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatments were used. The only difference in the animals was their genotype, with the mutants being liver-conditional knockouts for Pdss2.
| | Sample_growth_protocol_ch1 | Mutant and control animals were grown in separate cages in the University of Pennsylvania animal facility until time of sacrifice. Food was removed from the cages 3 hours prior to sacrifice by cervical dislocation without anesthesia.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol reagent was used according to the manufacturer's instructions to extract total RNA from flash-frozen liver aliquots
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Revision 5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430_2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Probe-level measurements were normalized, summarized and log2-transformed by dCHIP software using perfect-match-only and invariant-set methods.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eric,F.,Rappaport
| | Sample_contact_laboratory | Room 906
| | Sample_contact_department | Abramson Research Center
| | Sample_contact_institute | Children's Hospital of Philadelphia
| | Sample_contact_address | 3516 Civinc Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276433/suppl/GSM276433.CEL.gz
| | Sample_series_id | GSE10904
| | Sample_data_row_count | 45101
| | |
|
GSM276434 | GPL1261 |
|
Mutant, biological rep2
|
Alb69 mutant mouse
|
Genotype: B6.Alb/cre,Pdss2loxP/loxP(Liver-conditional knock-out)
Age: 178-day-old male
40 mg liver used for RNA extraction
|
Gene expression data from mutant M. musculus
|
| Sample_geo_accession | GSM276434
| | Sample_status | Public on Apr 30 2008
| | Sample_submission_date | Mar 20 2008
| | Sample_last_update_date | Mar 20 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatments were used. The only difference in the animals was their genotype, with the mutants being liver-conditional knockouts for Pdss2.
| | Sample_growth_protocol_ch1 | Mutant and control animals were grown in separate cages in the University of Pennsylvania animal facility until time of sacrifice. Food was removed from the cages 3 hours prior to sacrifice by cervical dislocation without anesthesia.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol reagent was used according to the manufacturer's instructions to extract total RNA from flash-frozen liver aliquots
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Revision 5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430_2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Probe-level measurements were normalized, summarized and log2-transformed by dCHIP software using perfect-match-only and invariant-set methods.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eric,F.,Rappaport
| | Sample_contact_laboratory | Room 906
| | Sample_contact_department | Abramson Research Center
| | Sample_contact_institute | Children's Hospital of Philadelphia
| | Sample_contact_address | 3516 Civinc Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276434/suppl/GSM276434.CEL.gz
| | Sample_series_id | GSE10904
| | Sample_data_row_count | 45101
| | |
|
GSM276435 | GPL1261 |
|
Mutant, biological rep3
|
Alb73 mutant mouse
|
Genotype: B6.Alb/cre,Pdss2loxP/loxP(Liver-conditional knock-out)
Age: 224-day-old male
40 mg liver used for RNA extraction
|
Gene expression data from mutant M. musculus
|
| Sample_geo_accession | GSM276435
| | Sample_status | Public on Apr 30 2008
| | Sample_submission_date | Mar 20 2008
| | Sample_last_update_date | Mar 20 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatments were used. The only difference in the animals was their genotype, with the mutants being liver-conditional knockouts for Pdss2.
| | Sample_growth_protocol_ch1 | Mutant and control animals were grown in separate cages in the University of Pennsylvania animal facility until time of sacrifice. Food was removed from the cages 3 hours prior to sacrifice by cervical dislocation without anesthesia.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol reagent was used according to the manufacturer's instructions to extract total RNA from flash-frozen liver aliquots
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Revision 5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430_2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Probe-level measurements were normalized, summarized and log2-transformed by dCHIP software using perfect-match-only and invariant-set methods.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eric,F.,Rappaport
| | Sample_contact_laboratory | Room 906
| | Sample_contact_department | Abramson Research Center
| | Sample_contact_institute | Children's Hospital of Philadelphia
| | Sample_contact_address | 3516 Civinc Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276435/suppl/GSM276435.CEL.gz
| | Sample_series_id | GSE10904
| | Sample_data_row_count | 45101
| | |
|
GSM276436 | GPL1261 |
|
Wildtype, biological rep1
|
lox62 control mouse
|
Genotype: B6.Pdss2loxP/loxP
Age: 209-day-old male (concurrent control to Alb73)
90 mg liver used for RNA extraction
|
Gene expression data from wildtype M. musculus
|
| Sample_geo_accession | GSM276436
| | Sample_status | Public on Apr 30 2008
| | Sample_submission_date | Mar 20 2008
| | Sample_last_update_date | Mar 20 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatments were used. The only difference in the animals was their genotype, with the mutants being liver-conditional knockouts for Pdss2.
| | Sample_growth_protocol_ch1 | Mutant and control animals were grown in separate cages in the University of Pennsylvania animal facility until time of sacrifice. Food was removed from the cages 3 hours prior to sacrifice by cervical dislocation without anesthesia.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol reagent was used according to the manufacturer's instructions to extract total RNA from flash-frozen liver aliquots
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Revision 5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430_2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Probe-level measurements were normalized, summarized and log2-transformed by dCHIP software using perfect-match-only and invariant-set methods.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eric,F.,Rappaport
| | Sample_contact_laboratory | Room 906
| | Sample_contact_department | Abramson Research Center
| | Sample_contact_institute | Children's Hospital of Philadelphia
| | Sample_contact_address | 3516 Civinc Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276436/suppl/GSM276436.CEL.gz
| | Sample_series_id | GSE10904
| | Sample_data_row_count | 45101
| | |
|
GSM276437 | GPL1261 |
|
Wildtype, biological rep2
|
lox67 control mouse
|
Genotype: B6.Pdss2loxP/loxP
Age: 213-day-old male
40 mg liver used for RNA extraction
|
Gene expression data from wildtype M. musculus
|
| Sample_geo_accession | GSM276437
| | Sample_status | Public on Apr 30 2008
| | Sample_submission_date | Mar 20 2008
| | Sample_last_update_date | Mar 20 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatments were used. The only difference in the animals was their genotype, with the mutants being liver-conditional knockouts for Pdss2.
| | Sample_growth_protocol_ch1 | Mutant and control animals were grown in separate cages in the University of Pennsylvania animal facility until time of sacrifice. Food was removed from the cages 3 hours prior to sacrifice by cervical dislocation without anesthesia.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol reagent was used according to the manufacturer's instructions to extract total RNA from flash-frozen liver aliquots
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Revision 5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430_2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Probe-level measurements were normalized, summarized and log2-transformed by dCHIP software using perfect-match-only and invariant-set methods.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eric,F.,Rappaport
| | Sample_contact_laboratory | Room 906
| | Sample_contact_department | Abramson Research Center
| | Sample_contact_institute | Children's Hospital of Philadelphia
| | Sample_contact_address | 3516 Civinc Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276437/suppl/GSM276437.CEL.gz
| | Sample_series_id | GSE10904
| | Sample_data_row_count | 45101
| | |
|
GSM276438 | GPL1261 |
|
Wildtype, biological rep3
|
lox95 control mouse
|
Genotype: B6.Pdss2loxP/loxP
Age: 140-day-old male
40 mg liver used for RNA extraction
|
Gene expression data from wildtype M. musculus
|
| Sample_geo_accession | GSM276438
| | Sample_status | Public on Apr 30 2008
| | Sample_submission_date | Mar 20 2008
| | Sample_last_update_date | Mar 20 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | No treatments were used. The only difference in the animals was their genotype, with the mutants being liver-conditional knockouts for Pdss2.
| | Sample_growth_protocol_ch1 | Mutant and control animals were grown in separate cages in the University of Pennsylvania animal facility until time of sacrifice. Food was removed from the cages 3 hours prior to sacrifice by cervical dislocation without anesthesia.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol reagent was used according to the manufacturer's instructions to extract total RNA from flash-frozen liver aliquots
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Revision 5, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430_2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | Probe-level measurements were normalized, summarized and log2-transformed by dCHIP software using perfect-match-only and invariant-set methods.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eric,F.,Rappaport
| | Sample_contact_laboratory | Room 906
| | Sample_contact_department | Abramson Research Center
| | Sample_contact_institute | Children's Hospital of Philadelphia
| | Sample_contact_address | 3516 Civinc Center Blvd
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19104
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276438/suppl/GSM276438.CEL.gz
| | Sample_series_id | GSE10904
| | Sample_data_row_count | 45101
| | |
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