Result

Search results for the GEO ID: GSE10915

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM276717

GPL339

ob/ob Leptin treated-1

ob/ob Leptin treated-1 lung

1 tissue: Lung

ob/ob Leptin treated Lung gene expression

Sample_geo_accession	GSM276717
Sample_status	Public on Jun 01 2008
Sample_submission_date	Mar 21 2008
Sample_last_update_date	Mar 24 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Leptin treatment
Sample_growth_protocol_ch1	no growth protocol
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	ENZO BioArray HighYield RNA Transcript Labeling Kit
Sample_label_protocol_ch1	cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module, cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix Small Sample Labeling Protocol vII, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manul- Affymetrix)
Sample_scan_protocol	Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
Sample_data_processing	Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1. Analysis of differentially expressed transcripts was accomplished using a pairwise comparative analysis (Affy Data Mining Tool v3.1; Affymetrix). 3 sets ob/ob leptin-treated 1 month x 3 sets ob/ob saline-treated 1 month comparison (1.5 fold cutoff (FC), Mann-Whitney test < 0.05, 6 out of 9 comparisons).
Sample_platform_id	GPL339
Sample_contact_name	vikas,,misra
Sample_contact_email	vmisra@jhsph.edu
Sample_contact_laboratory	Clarke G Tankersley
Sample_contact_department	Environmental health sciences
Sample_contact_institute	Johns Hopkins University School of public health
Sample_contact_address	615 N. Wolfe street, E6609
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21205
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276717/suppl/GSM276717.CEL.gz
Sample_series_id	GSE10915
Sample_data_row_count	22690

GSM276718

GPL339

ob/ob Leptin treated-2

ob/ob Leptin treated-2 lung

1 tissue: Lung

ob/ob Leptin treated Lung gene expression

Sample_geo_accession	GSM276718
Sample_status	Public on Jun 01 2008
Sample_submission_date	Mar 21 2008
Sample_last_update_date	Mar 24 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Leptin treatment
Sample_growth_protocol_ch1	no growth protocol
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	ENZO BioArray HighYield RNA Transcript Labeling Kit
Sample_label_protocol_ch1	cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module, cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix Small Sample Labeling Protocol vII, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manul- Affymetrix)
Sample_scan_protocol	Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
Sample_data_processing	Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1. Analysis of differentially expressed transcripts was accomplished using a pairwise comparative analysis (Affy Data Mining Tool v3.1; Affymetrix). 3 sets ob/ob leptin-treated 1 month x 3 sets ob/ob saline-treated 1 month comparison (1.5 fold cutoff (FC), Mann-Whitney test < 0.05, 6 out of 9 comparisons).
Sample_platform_id	GPL339
Sample_contact_name	vikas,,misra
Sample_contact_email	vmisra@jhsph.edu
Sample_contact_laboratory	Clarke G Tankersley
Sample_contact_department	Environmental health sciences
Sample_contact_institute	Johns Hopkins University School of public health
Sample_contact_address	615 N. Wolfe street, E6609
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21205
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276718/suppl/GSM276718.CEL.gz
Sample_series_id	GSE10915
Sample_data_row_count	22690

GSM276719

GPL339

ob/ob Leptin treated-3

ob/ob Leptin treated-3 lung

1 tissue: Lung

ob/ob Leptin treated Lung gene expression

Sample_geo_accession	GSM276719
Sample_status	Public on Jun 01 2008
Sample_submission_date	Mar 21 2008
Sample_last_update_date	Mar 24 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Leptin treatment
Sample_growth_protocol_ch1	no growth protocol
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	ENZO BioArray HighYield RNA Transcript Labeling Kit
Sample_label_protocol_ch1	cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module, cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix Small Sample Labeling Protocol vII, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manul- Affymetrix)
Sample_scan_protocol	Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
Sample_data_processing	Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1. Analysis of differentially expressed transcripts was accomplished using a pairwise comparative analysis (Affy Data Mining Tool v3.1; Affymetrix). 3 sets ob/ob leptin-treated 1 month x 3 sets ob/ob saline-treated 1 month comparison (1.5 fold cutoff (FC), Mann-Whitney test < 0.05, 6 out of 9 comparisons).
Sample_platform_id	GPL339
Sample_contact_name	vikas,,misra
Sample_contact_email	vmisra@jhsph.edu
Sample_contact_laboratory	Clarke G Tankersley
Sample_contact_department	Environmental health sciences
Sample_contact_institute	Johns Hopkins University School of public health
Sample_contact_address	615 N. Wolfe street, E6609
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21205
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276719/suppl/GSM276719.CEL.gz
Sample_series_id	GSE10915
Sample_data_row_count	22690

GSM276720

GPL339

ob/ob Saline treated-1

ob/ob Saline treated-1 lung

1 tissue: Lung

ob/ob Saline treated Lung gene expression

Sample_geo_accession	GSM276720
Sample_status	Public on Jun 01 2008
Sample_submission_date	Mar 21 2008
Sample_last_update_date	Mar 24 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Saline treatment
Sample_growth_protocol_ch1	no growth protocol
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	ENZO BioArray HighYield RNA Transcript Labeling Kit
Sample_label_protocol_ch1	cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module, cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix Small Sample Labeling Protocol vII, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manul- Affymetrix)
Sample_scan_protocol	Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
Sample_data_processing	Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1. Analysis of differentially expressed transcripts was accomplished using a pairwise comparative analysis (Affy Data Mining Tool v3.1; Affymetrix). 3 sets ob/ob leptin-treated 1 month x 3 sets ob/ob saline-treated 1 month comparison (1.5 fold cutoff (FC), Mann-Whitney test < 0.05, 6 out of 9 comparisons).
Sample_platform_id	GPL339
Sample_contact_name	vikas,,misra
Sample_contact_email	vmisra@jhsph.edu
Sample_contact_laboratory	Clarke G Tankersley
Sample_contact_department	Environmental health sciences
Sample_contact_institute	Johns Hopkins University School of public health
Sample_contact_address	615 N. Wolfe street, E6609
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21205
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276720/suppl/GSM276720.CEL.gz
Sample_series_id	GSE10915
Sample_data_row_count	22690

GSM276721

GPL339

ob/ob Saline treated-2

ob/ob Saline treated-2 lung

1 tissue: Lung

ob/ob Saline treated Lung gene expression

Sample_geo_accession	GSM276721
Sample_status	Public on Jun 01 2008
Sample_submission_date	Mar 21 2008
Sample_last_update_date	Mar 24 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Saline treatment
Sample_growth_protocol_ch1	no growth protocol
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	ENZO BioArray HighYield RNA Transcript Labeling Kit
Sample_label_protocol_ch1	cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module, cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix Small Sample Labeling Protocol vII, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manul- Affymetrix)
Sample_scan_protocol	Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
Sample_data_processing	Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1. Analysis of differentially expressed transcripts was accomplished using a pairwise comparative analysis (Affy Data Mining Tool v3.1; Affymetrix). 3 sets ob/ob leptin-treated 1 month x 3 sets ob/ob saline-treated 1 month comparison (1.5 fold cutoff (FC), Mann-Whitney test < 0.05, 6 out of 9 comparisons).
Sample_platform_id	GPL339
Sample_contact_name	vikas,,misra
Sample_contact_email	vmisra@jhsph.edu
Sample_contact_laboratory	Clarke G Tankersley
Sample_contact_department	Environmental health sciences
Sample_contact_institute	Johns Hopkins University School of public health
Sample_contact_address	615 N. Wolfe street, E6609
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21205
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276721/suppl/GSM276721.CEL.gz
Sample_series_id	GSE10915
Sample_data_row_count	22690

GSM276722

GPL339

ob/ob Saline treated-3

ob/ob Saline treated-3 lung

1 tissue: Lung

ob/ob Saline treated Lung gene expression

Sample_geo_accession	GSM276722
Sample_status	Public on Jun 01 2008
Sample_submission_date	Mar 21 2008
Sample_last_update_date	Mar 24 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Saline treatment
Sample_growth_protocol_ch1	no growth protocol
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	ENZO BioArray HighYield RNA Transcript Labeling Kit
Sample_label_protocol_ch1	cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module, cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix Small Sample Labeling Protocol vII, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manul- Affymetrix)
Sample_scan_protocol	Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
Sample_data_processing	Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1. Analysis of differentially expressed transcripts was accomplished using a pairwise comparative analysis (Affy Data Mining Tool v3.1; Affymetrix). 3 sets ob/ob leptin-treated 1 month x 3 sets ob/ob saline-treated 1 month comparison (1.5 fold cutoff (FC), Mann-Whitney test < 0.05, 6 out of 9 comparisons).
Sample_platform_id	GPL339
Sample_contact_name	vikas,,misra
Sample_contact_email	vmisra@jhsph.edu
Sample_contact_laboratory	Clarke G Tankersley
Sample_contact_department	Environmental health sciences
Sample_contact_institute	Johns Hopkins University School of public health
Sample_contact_address	615 N. Wolfe street, E6609
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21205
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM276nnn/GSM276722/suppl/GSM276722.CEL.gz
Sample_series_id	GSE10915
Sample_data_row_count	22690

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