Search results for the GEO ID: GSE10946  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM277438 | GPL570 |
|
cumulus_nonPCOS_Lean_rep1
|
luteinized human cumulus cells, cultured 48h, nonPCOS, Lean, rep1
|
Gender: Female, Age: 28, BMI: 17, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277438
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277438/suppl/GSM277438.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277439 | GPL570 |
|
cumulus_nonPCOS_Lean_rep2
|
luteinized human cumulus cells, cultured 48h, nonPCOS, Lean, rep2
|
Gender: Female, Age: 27, BMI: 21, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277439
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277439/suppl/GSM277439.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277440 | GPL570 |
|
cumulus_nonPCOS_Lean_rep3
|
luteinized human cumulus cells, cultured 48h, nonPCOS, Lean, rep3
|
Gender: Female, Age: 30, BMI: 24, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277440
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277440/suppl/GSM277440.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277441 | GPL570 |
|
cumulus_nonPCOS_Lean_rep4
|
luteinized human cumulus cells, cultured 48h, nonPCOS, Lean, rep4
|
Gender: Female, Age: 25, BMI: 16, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277441
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277441/suppl/GSM277441.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277442 | GPL570 |
|
cumulus_nonPCOS_Lean_rep5
|
luteinized human cumulus cells, cultured 48h, nonPCOS, Lean, rep5
|
Gender: Female, Age: 31, BMI: 21, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277442
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277442/suppl/GSM277442.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277443 | GPL570 |
|
cumulus_nonPCOS_Lean_rep6
|
luteinized human cumulus cells, cultured 48h, nonPCOS, Lean, rep6
|
Gender: Female, Age: 29, BMI: 22, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277443
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277443/suppl/GSM277443.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277444 | GPL570 |
|
cumulus_nonPCOS_Obese_rep1
|
luteinized human cumulus cells, cultured 48h, nonPCOS, Obese, rep1
|
Gender: Female, Age: 35, BMI: 27, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277444
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277444/suppl/GSM277444.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277445 | GPL570 |
|
cumulus_nonPCOS_Obese_rep2
|
luteinized human cumulus cells, cultured 48h, nonPCOS, Obese, rep2
|
Gender: Female, Age: 33, BMI: 33, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277445
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277445/suppl/GSM277445.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277446 | GPL570 |
|
cumulus_nonPCOS_Obese_rep3
|
luteinized human cumulus cells, cultured 48h, nonPCOS, Obese, rep3
|
Gender: Female, Age: 28, BMI: 34, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277446
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277446/suppl/GSM277446.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277447 | GPL570 |
|
cumulus_nonPCOS_Obese_rep4
|
luteinized human cumulus cells, cultured 48h, nonPCOS, Obese, rep4
|
Gender: Female, Age: 32, BMI: 30, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277447
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277447/suppl/GSM277447.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277448 | GPL570 |
|
cumulus_nonPCOS_Obese_rep5
|
luteinized human cumulus cells, cultured 48h, nonPCOS, Obese, rep5
|
Gender: Female, Age: 28, BMI: 30, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277448
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277448/suppl/GSM277448.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277449 | GPL570 |
|
cumulus_PCOS_Lean_rep1
|
luteinized human cumulus cells, cultured 48h, PCOS, Lean, rep1
|
Gender: Female, Age: 27, BMI: 19, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277449
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277449/suppl/GSM277449.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277450 | GPL570 |
|
cumulus_PCOS_Lean_rep2
|
luteinized human cumulus cells, cultured 48h, PCOS, Lean, rep2
|
Gender: Female, Age: 35, BMI: 22, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277450
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277450/suppl/GSM277450.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277451 | GPL570 |
|
cumulus_PCOS_Lean_rep3
|
luteinized human cumulus cells, cultured 48h, PCOS, Lean, rep3
|
Gender: Female, Age: 25, BMI: 23, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277451
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277451/suppl/GSM277451.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277452 | GPL570 |
|
cumulus_PCOS_Lean_rep4
|
luteinized human cumulus cells, cultured 48h, PCOS, Lean, rep4
|
Gender: Female, Age: 32, BMI: 25, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277452
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277452/suppl/GSM277452.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277453 | GPL570 |
|
cumulus_PCOS_Lean_rep5
|
luteinized human cumulus cells, cultured 48h, PCOS, Lean, rep5
|
Gender: Female, Age: 28, BMI: 17.1, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277453
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277453/suppl/GSM277453.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277454 | GPL570 |
|
cumulus_PCOS_Obese_rep1
|
luteinized human cumulus cells, cultured 48h, PCOS, Obese, rep1
|
Gender: Female, Age: 25, BMI: 31, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277454
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277454/suppl/GSM277454.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277455 | GPL570 |
|
cumulus_PCOS_Obese_rep2
|
luteinized human cumulus cells, cultured 48h, PCOS, Obese, rep2
|
Gender: Female, Age: 358, BMI: 30, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277455
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277455/suppl/GSM277455.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277456 | GPL570 |
|
cumulus_PCOS_Obese_rep3
|
luteinized human cumulus cells, cultured 48h, PCOS, Obese, rep3
|
Gender: Female, Age: 37, BMI: 31, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277456
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277456/suppl/GSM277456.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277457 | GPL570 |
|
cumulus_PCOS_Obese_rep4
|
luteinized human cumulus cells, cultured 48h, PCOS, Obese, rep4
|
Gender: Female, Age: 31, BMI: 37, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277457
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277457/suppl/GSM277457.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277458 | GPL570 |
|
cumulus_PCOS_Obese_rep5
|
luteinized human cumulus cells, cultured 48h, PCOS, Obese, rep5
|
Gender: Female, Age: 27, BMI: 29, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277458
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277458/suppl/GSM277458.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277459 | GPL570 |
|
cumulus_PCOS_Obese_rep6
|
luteinized human cumulus cells, cultured 48h, PCOS, Obese, rep6
|
Gender: Female, Age: 25, BMI: 32, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277459
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277459/suppl/GSM277459.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
|
GSM277460 | GPL570 |
|
cumulus_PCOS_Obese_rep7
|
luteinized human cumulus cells, cultured 48h, PCOS, Obese, rep7
|
Gender: Female, Age: 37, BMI: 31, Tissue: luteinized cumulus cells
|
All the participants included in the study were women undergoing In-Vitro Fertilization with intracytoplasmatic sperm injection (IVF-ICSI) at the IVF unit. The IVF-ICSI cycles included in the study were conducted according to the long mid luteal GnRH agonist (Diphereline 3.75 mg PharmaBiotech, France) protocol. Controlled ovarian hyperstimulation was conducted with either recombinant follicular stimulating hormone (rFSH- Follitropin Alfa, Serono, UK) or human menopausal gonadotropin (hMG - Menotropin, Ferring, Germany). Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette.
|
| Sample_geo_accession | GSM277460
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Patients were treated with gonadotropins
| | Sample_growth_protocol_ch1 | Cells were cultured for 48h in the original IVF plate, washed 3 times with DMEM/Ham's F12 (1:1) supplemented with penicillin, streptomycin and amphotericin and resuspended in the same medium supplemented with 10% Fetal calf serum. Cells were incubated for 48h at 37°C with 5% CO2, medium was replaced after 24h.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Trizol (Molecular Research) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Small Sample Target Labeling Assay v.2' protocol from 50 ng total RNA from Affymetrix, (Santa Clara, CA)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Agilent GeneArray Scanner (Affymetrix)
| | Sample_data_processing | Data was preprocessed using Robust Multiarray Average (RMA) with default parameters, through the Affy package of bioconductor.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Kenigsberg
| | Sample_contact_email | shlomit.kenig@gmail.com
| | Sample_contact_laboratory | Ovarian Biology Research
| | Sample_contact_department | Research
| | Sample_contact_institute | Create Fertility Centre
| | Sample_contact_address | 790 Bay st.
| | Sample_contact_city | Toronto
| | Sample_contact_state | Ontario
| | Sample_contact_zip/postal_code | M4N1S8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277460/suppl/GSM277460.CEL.gz
| | Sample_series_id | GSE10946
| | Sample_data_row_count | 54675
| | |
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