Search results for the GEO ID: GSE10953  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM277558 | GPL339 |
|
G93A 60 days sample 1
|
G93A mouse 1261
|
Transgenic male mouse 60 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277558
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277558/suppl/GSM277558.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277559 | GPL339 |
|
G93A 60 days sample 2
|
G93A mouse 1262
|
Transgenic male mouse 60 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277559
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277559/suppl/GSM277559.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277560 | GPL339 |
|
G93A 60 days sample 3
|
G93A mouse 1266
|
Transgenic male mouse 60 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277560
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277560/suppl/GSM277560.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277561 | GPL339 |
|
Control 60 days sample 1
|
G93A non transgenic littermate 1263
|
Male mouse 60 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277561
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277561/suppl/GSM277561.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277562 | GPL339 |
|
Control 60 days sample 2
|
G93A non transgenic littermate 1264
|
Male mouse 60 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277562
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277562/suppl/GSM277562.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277563 | GPL339 |
|
Control 60 days sample 3
|
G93A non transgenic littermate 1265
|
Male mouse 60 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277563
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277563/suppl/GSM277563.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277564 | GPL339 |
|
G93A 90 days sample 1
|
G93A mouse 1645
|
Transgenic male mouse 90 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277564
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277564/suppl/GSM277564.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277565 | GPL339 |
|
G93A 90 days sample 2
|
G93A mouse 1647
|
Transgenic male mouse 90 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277565
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277565/suppl/GSM277565.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277566 | GPL339 |
|
G93A 90 days sample 3
|
G93A mouse 1423
|
Transgenic male mouse 90 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277566
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277566/suppl/GSM277566.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277567 | GPL339 |
|
Control 90 days sample 1
|
G93A non transgenic littermate 1642
|
Male mouse 90 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277567
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277567/suppl/GSM277567.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277568 | GPL339 |
|
Control 90 days sample 2
|
G93A non transgenic littermate 1644
|
Male mouse 90 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277568
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277568/suppl/GSM277568.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277569 | GPL339 |
|
Control 90 days sample 3
|
G93A non transgenic littermate 1424
|
Male mouse 90 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277569
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277569/suppl/GSM277569.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277570 | GPL339 |
|
G93A 120 days sample 1
|
G93A mouse 799
|
Transgenic male mouse 120 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277570
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277570/suppl/GSM277570.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277571 | GPL339 |
|
G93A 120 days sample 2
|
G93A mouse 801
|
Transgenic male mouse 120 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277571
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277571/suppl/GSM277571.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277572 | GPL339 |
|
G93A 120 days sample 3
|
G93A mouse 802
|
Transgenic male mouse 120 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277572
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277572/suppl/GSM277572.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277573 | GPL339 |
|
Control 120 days sample 1
|
G93A non transgenic littermate 800
|
Male mouse 120 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277573
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277573/suppl/GSM277573.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277574 | GPL339 |
|
Control 120 days sample 2
|
G93A non transgenic littermate 803
|
Male mouse 120 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277574
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277574/suppl/GSM277574.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
GSM277575 | GPL339 |
|
Control 120 days sample 3
|
G93A non transgenic littermate 805
|
Male mouse 120 day old
|
Gene expression data from murine motor neurons
|
| Sample_geo_accession | GSM277575
| | Sample_status | Public on Mar 27 2008
| | Sample_submission_date | Mar 26 2008
| | Sample_last_update_date | Mar 26 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | 10 um lumbar spinal cord frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| | Sample_extract_protocol_ch1 | From each animal 1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience)
| | Sample_extract_protocol_ch1 | PicoPure RNA Extraction Kit was used for RNA extraction according to manufacturers' instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylation of antisense RNA carried out according to Affymetrix protocol
| | Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Mouse Genome 430A GeneChip in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post hybridisation stringency washing was done using the Affymetrix fluidics station again following the protocol outlined in the Affymetrix instructions
| | Sample_scan_protocol | the GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the GCOS software package to prepare the .CEL files
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
| | Sample_platform_id | GPL339
| | Sample_contact_name | Paul,Roy,Heath
| | Sample_contact_email | p.heath@sheffield.ac.uk
| | Sample_contact_phone | +44 114 2222254
| | Sample_contact_laboratory | Heath
| | Sample_contact_department | Academic Neurology Unit
| | Sample_contact_institute | University of Sheffield
| | Sample_contact_address | SITraN, 385a Glossop Road
| | Sample_contact_city | Sheffield
| | Sample_contact_state | S. Yorks
| | Sample_contact_zip/postal_code | S10 2HQ
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM277nnn/GSM277575/suppl/GSM277575.CEL.gz
| | Sample_series_id | GSE10953
| | Sample_data_row_count | 22690
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
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