Search results for the GEO ID: GSE11091  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM280163 | GPL341 |
|
zincsulfate-Ctrl1-heart
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-Ctrl1-heart
|
| Sample_geo_accession | GSM280163
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280163/suppl/GSM280163.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280163/suppl/GSM280163.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280164 | GPL341 |
|
zincsulfate-Ctrl2-heart
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-Ctrl2-heart
|
| Sample_geo_accession | GSM280164
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280164/suppl/GSM280164.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280164/suppl/GSM280164.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280165 | GPL341 |
|
zincsulfate-Ctrl3-heart
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-Ctrl3-heart
|
| Sample_geo_accession | GSM280165
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280165/suppl/GSM280165.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280165/suppl/GSM280165.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280166 | GPL341 |
|
zincsulfate-Ctrl4-heart
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-Ctrl4-heart
|
| Sample_geo_accession | GSM280166
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280166/suppl/GSM280166.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280166/suppl/GSM280166.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280167 | GPL341 |
|
zincsulfate-Ctrl5-heart
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-Ctrl5-heart
|
| Sample_geo_accession | GSM280167
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280167/suppl/GSM280167.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280167/suppl/GSM280167.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280168 | GPL341 |
|
zincsulfate-Ctrl6-heart
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-Ctrl6-heart
|
| Sample_geo_accession | GSM280168
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280168/suppl/GSM280168.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280168/suppl/GSM280168.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280169 | GPL341 |
|
zincsulfate-10ug-heart-21
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-10ug-heart-21
|
| Sample_geo_accession | GSM280169
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280169/suppl/GSM280169.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280169/suppl/GSM280169.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280170 | GPL341 |
|
zincsulfate-10ug-heart-22
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-10ug-heart-22
|
| Sample_geo_accession | GSM280170
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280170/suppl/GSM280170.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280170/suppl/GSM280170.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280171 | GPL341 |
|
zincsulfate-10ug-heart-23
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-10ug-heart-23
|
| Sample_geo_accession | GSM280171
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280171/suppl/GSM280171.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280171/suppl/GSM280171.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280172 | GPL341 |
|
zincsulfate-10ug-heart-24
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-10ug-heart-24
|
| Sample_geo_accession | GSM280172
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280172/suppl/GSM280172.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280172/suppl/GSM280172.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280173 | GPL341 |
|
zincsulfate-10ug-heart-25
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-10ug-heart-25
|
| Sample_geo_accession | GSM280173
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280173/suppl/GSM280173.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280173/suppl/GSM280173.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280174 | GPL341 |
|
zincsulfate-10ug-heart-26
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-10ug-heart-26
|
| Sample_geo_accession | GSM280174
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280174/suppl/GSM280174.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280174/suppl/GSM280174.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280175 | GPL341 |
|
zincsulfate-30ug-heart-41
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-30ug-heart-41
|
| Sample_geo_accession | GSM280175
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280175/suppl/GSM280175.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280175/suppl/GSM280175.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280176 | GPL341 |
|
zincsulfate-30ug-heart-42
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-30ug-heart-42
|
| Sample_geo_accession | GSM280176
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280176/suppl/GSM280176.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280176/suppl/GSM280176.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280177 | GPL341 |
|
zincsulfate-30ug-heart-43
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-30ug-heart-43
|
| Sample_geo_accession | GSM280177
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280177/suppl/GSM280177.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280177/suppl/GSM280177.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280178 | GPL341 |
|
zincsulfate-30ug-heart-44
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-30ug-heart-44
|
| Sample_geo_accession | GSM280178
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280178/suppl/GSM280178.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280178/suppl/GSM280178.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280179 | GPL341 |
|
zincsulfate-30ug-heart-45
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-30ug-heart-45
|
| Sample_geo_accession | GSM280179
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280179/suppl/GSM280179.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280179/suppl/GSM280179.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280180 | GPL341 |
|
zincsulfate-30ug-heart-46
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-30ug-heart-46
|
| Sample_geo_accession | GSM280180
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280180/suppl/GSM280180.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280180/suppl/GSM280180.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280181 | GPL341 |
|
zincsulfate-100ug-heart-61
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-100ug-heart-61
|
| Sample_geo_accession | GSM280181
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280181/suppl/GSM280181.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280181/suppl/GSM280181.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280182 | GPL341 |
|
zincsulfate-100ug-heart-62
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-100ug-heart-62
|
| Sample_geo_accession | GSM280182
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280182/suppl/GSM280182.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280182/suppl/GSM280182.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280183 | GPL341 |
|
zincsulfate-100ug-heart-63
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-100ug-heart-63
|
| Sample_geo_accession | GSM280183
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280183/suppl/GSM280183.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280183/suppl/GSM280183.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280184 | GPL341 |
|
zincsulfate-100ug-heart-64
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-100ug-heart-64
|
| Sample_geo_accession | GSM280184
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280184/suppl/GSM280184.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280184/suppl/GSM280184.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280185 | GPL341 |
|
zincsulfate-100ug-heart-65
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-100ug-heart-65
|
| Sample_geo_accession | GSM280185
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280185/suppl/GSM280185.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280185/suppl/GSM280185.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
|
GSM280186 | GPL341 |
|
zincsulfate-100ug-heart-66
|
Rat whole heart-16 weeks
|
15 per group) according to body weight, and exposed by nose-only inhalation (Ledbetter et al., 1998) to either filtered air or aerosolized zinc sulfate (10, 30 or 100 ug Zn/m3) for 5 h/day, 3 days/week for 16 weeks. The aerosol was generated by aerosolizing solutions of zinc sulfate heptahydrate (Sigma Aldrich Chemicals), mixed at different starting concentrations with a TSI Model 3076 atomizer operating at 30 PSIG. The output was mixed with dry air and directed to the exposure chamber (air flow of 98-105 liters/minute). Chamber concentration data was determined by gravimetric analysis, with Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) to determine zinc concentration.
|
zincsulfate-100ug-heart-66
|
| Sample_geo_accession | GSM280186
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Target RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) and further purified using the RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using Affymetrix Scanner 3000G
| | Sample_data_processing | The data were analyzed with Rosetta Resolver 6.0 using Affymetrix default profile builder analysis settings.
| | Sample_platform_id | GPL341
| | Sample_contact_name | beena,,vallanat
| | Sample_contact_email | vallanat.beena@epa.gov
| | Sample_contact_institute | EPA
| | Sample_contact_address | 109 Tw Alexanser Dr
| | Sample_contact_city | Research Triangle Park
| | Sample_contact_zip/postal_code | 27711
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280186/suppl/GSM280186.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280186/suppl/GSM280186.CHP.gz
| | Sample_series_id | GSE11091
| | Sample_data_row_count | 15923
| | |
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