Search results for the GEO ID: GSE11110  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM280466 | GPL1261 |
|
Adult murine BM, hCD25- CLP (LIN-B220-CD19-IL7R+FLT3+SCA1lowKITlowhCD25-) #1
|
Adult murine BM from 10-15 weeks old mice
|
Wild type C57bl/6
|
Gene expression data from LSKCD34-Flt3- cells FACS sorted from adult BM
|
| Sample_geo_accession | GSM280466
| | Sample_status | Public on Jun 29 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Apr 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were directly sorted into buffer RLT and RNA prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard AffymetrixTM; Small Sample Labelling Protocol v.2, with the exception that the second round of in vitro transcription (IVT) was performed using AffymetrixTM GeneChipTM Expression 3’amplification kit.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
| | Sample_data_processing | Expression values were calculated by RMA express using background adjustment and quantile normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Robert,,Månsson
| | Sample_contact_email | robert.mansson@ki.se
| | Sample_contact_department | Hemapotoietic stem cell biology
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Klinikgatan 26, BMC B12
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 221 84
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280466/suppl/GSM280466.CEL.gz
| | Sample_series_id | GSE11110
| | Sample_data_row_count | 45101
| | |
|
GSM280467 | GPL1261 |
|
Adult murine BM, hCD25- CLP (LIN-B220-CD19-IL7R+FLT3+SCA1lowKITlowhCD25-) #2
|
Adult murine BM from 10-15 weeks old mice
|
Wild type C57bl/6
|
Gene expression data from LSKCD34-Flt3- cells FACS sorted from adult BM
|
| Sample_geo_accession | GSM280467
| | Sample_status | Public on Jun 29 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Apr 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were directly sorted into buffer RLT and RNA prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard AffymetrixTM; Small Sample Labelling Protocol v.2, with the exception that the second round of in vitro transcription (IVT) was performed using AffymetrixTM GeneChipTM Expression 3’amplification kit.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
| | Sample_data_processing | Expression values were calculated by RMA express using background adjustment and quantile normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Robert,,Månsson
| | Sample_contact_email | robert.mansson@ki.se
| | Sample_contact_department | Hemapotoietic stem cell biology
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Klinikgatan 26, BMC B12
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 221 84
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280467/suppl/GSM280467.CEL.gz
| | Sample_series_id | GSE11110
| | Sample_data_row_count | 45101
| | |
|
GSM280468 | GPL1261 |
|
Adult murine BM, hCD25- CLP (LIN-B220-CD19-IL7R+FLT3+SCA1lowKITlowhCD25-) #3
|
Adult murine BM from 10-15 weeks old mice
|
Wild type C57bl/6
|
Gene expression data from LSKCD34-Flt3- cells FACS sorted from adult BM
|
| Sample_geo_accession | GSM280468
| | Sample_status | Public on Jun 29 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Apr 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were directly sorted into buffer RLT and RNA prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard AffymetrixTM; Small Sample Labelling Protocol v.2, with the exception that the second round of in vitro transcription (IVT) was performed using AffymetrixTM GeneChipTM Expression 3’amplification kit.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
| | Sample_data_processing | Expression values were calculated by RMA express using background adjustment and quantile normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Robert,,Månsson
| | Sample_contact_email | robert.mansson@ki.se
| | Sample_contact_department | Hemapotoietic stem cell biology
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Klinikgatan 26, BMC B12
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 221 84
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280468/suppl/GSM280468.CEL.gz
| | Sample_series_id | GSE11110
| | Sample_data_row_count | 45101
| | |
|
GSM280469 | GPL1261 |
|
Adult murine BM, hCD25- CLP (LIN-B220-CD19-IL7R+FLT3+SCA1lowKITlowhCD25+) #1
|
Adult murine BM from 10-15 weeks old mice
|
Wild type C57bl/6
|
Gene expression data from LSKCD34-Flt3- cells FACS sorted from adult BM
|
| Sample_geo_accession | GSM280469
| | Sample_status | Public on Jun 29 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Apr 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were directly sorted into buffer RLT and RNA prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard AffymetrixTM; Small Sample Labelling Protocol v.2, with the exception that the second round of in vitro transcription (IVT) was performed using AffymetrixTM GeneChipTM Expression 3’amplification kit.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
| | Sample_data_processing | Expression values were calculated by RMA express using background adjustment and quantile normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Robert,,Månsson
| | Sample_contact_email | robert.mansson@ki.se
| | Sample_contact_department | Hemapotoietic stem cell biology
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Klinikgatan 26, BMC B12
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 221 84
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280469/suppl/GSM280469.CEL.gz
| | Sample_series_id | GSE11110
| | Sample_data_row_count | 45101
| | |
|
GSM280470 | GPL1261 |
|
Adult murine BM, hCD25+ CLP (LIN-B220-CD19-IL7R+FLT3+SCA1lowKITlowhCD25+) #2
|
Adult murine BM from 10-15 weeks old mice
|
Wild type C57bl/6
|
Gene expression data from LSKCD34-Flt3- cells FACS sorted from adult BM
|
| Sample_geo_accession | GSM280470
| | Sample_status | Public on Jun 29 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Apr 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were directly sorted into buffer RLT and RNA prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard AffymetrixTM; Small Sample Labelling Protocol v.2, with the exception that the second round of in vitro transcription (IVT) was performed using AffymetrixTM GeneChipTM Expression 3’amplification kit.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
| | Sample_data_processing | Expression values were calculated by RMA express using background adjustment and quantile normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Robert,,Månsson
| | Sample_contact_email | robert.mansson@ki.se
| | Sample_contact_department | Hemapotoietic stem cell biology
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Klinikgatan 26, BMC B12
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 221 84
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280470/suppl/GSM280470.CEL.gz
| | Sample_series_id | GSE11110
| | Sample_data_row_count | 45101
| | |
|
GSM280471 | GPL1261 |
|
Adult murine BM, pro-B cells (CD19+AA4.1+CD43low) #1
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Adult murine BM from 10-15 weeks old mice
|
Wild type C57bl/6
|
Gene expression data from LSKCD34-Flt3- cells FACS sorted from adult BM
|
| Sample_geo_accession | GSM280471
| | Sample_status | Public on Jun 29 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Apr 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were directly sorted into buffer RLT and RNA prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard AffymetrixTM; Small Sample Labelling Protocol v.2, with the exception that the second round of in vitro transcription (IVT) was performed using AffymetrixTM GeneChipTM Expression 3’amplification kit.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
| | Sample_data_processing | Expression values were calculated by RMA express using background adjustment and quantile normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Robert,,Månsson
| | Sample_contact_email | robert.mansson@ki.se
| | Sample_contact_department | Hemapotoietic stem cell biology
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Klinikgatan 26, BMC B12
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 221 84
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280471/suppl/GSM280471.CEL.gz
| | Sample_series_id | GSE11110
| | Sample_data_row_count | 45101
| | |
|
GSM280472 | GPL1261 |
|
Adult murine BM, pro-B cells (CD19+AA4.1+CD43low) #2
|
Adult murine BM from 10-15 weeks old mice
|
Wild type C57bl/6
|
Gene expression data from LSKCD34-Flt3- cells FACS sorted from adult BM
|
| Sample_geo_accession | GSM280472
| | Sample_status | Public on Jun 29 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Apr 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were directly sorted into buffer RLT and RNA prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard AffymetrixTM; Small Sample Labelling Protocol v.2, with the exception that the second round of in vitro transcription (IVT) was performed using AffymetrixTM GeneChipTM Expression 3’amplification kit.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
| | Sample_data_processing | Expression values were calculated by RMA express using background adjustment and quantile normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Robert,,Månsson
| | Sample_contact_email | robert.mansson@ki.se
| | Sample_contact_department | Hemapotoietic stem cell biology
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Klinikgatan 26, BMC B12
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 221 84
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280472/suppl/GSM280472.CEL.gz
| | Sample_series_id | GSE11110
| | Sample_data_row_count | 45101
| | |
|
GSM280473 | GPL1261 |
|
Adult murine BM, pro-B cells (CD19+B220+CD43-IgM-) #1
|
Adult murine BM from 10-15 weeks old mice
|
Wild type C57bl/6
|
Gene expression data from LSKCD34-Flt3- cells FACS sorted from adult BM
|
| Sample_geo_accession | GSM280473
| | Sample_status | Public on Jun 29 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Apr 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were directly sorted into buffer RLT and RNA prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard AffymetrixTM; Small Sample Labelling Protocol v.2, with the exception that the second round of in vitro transcription (IVT) was performed using AffymetrixTM GeneChipTM Expression 3’amplification kit.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
| | Sample_data_processing | Expression values were calculated by RMA express using background adjustment and quantile normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Robert,,Månsson
| | Sample_contact_email | robert.mansson@ki.se
| | Sample_contact_department | Hemapotoietic stem cell biology
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Klinikgatan 26, BMC B12
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 221 84
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280473/suppl/GSM280473.CEL.gz
| | Sample_series_id | GSE11110
| | Sample_data_row_count | 45101
| | |
|
GSM280474 | GPL1261 |
|
Adult murine BM, pro-B cells (CD19+B220+CD43-IgM-) #2
|
Adult murine BM from 10-15 weeks old mice
|
Wild type C57bl/6
|
Gene expression data from LSKCD34-Flt3- cells FACS sorted from adult BM
|
| Sample_geo_accession | GSM280474
| | Sample_status | Public on Jun 29 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Apr 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were directly sorted into buffer RLT and RNA prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard AffymetrixTM; Small Sample Labelling Protocol v.2, with the exception that the second round of in vitro transcription (IVT) was performed using AffymetrixTM GeneChipTM Expression 3’amplification kit.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
| | Sample_data_processing | Expression values were calculated by RMA express using background adjustment and quantile normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Robert,,Månsson
| | Sample_contact_email | robert.mansson@ki.se
| | Sample_contact_department | Hemapotoietic stem cell biology
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Klinikgatan 26, BMC B12
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 221 84
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280474/suppl/GSM280474.CEL.gz
| | Sample_series_id | GSE11110
| | Sample_data_row_count | 45101
| | |
|
GSM280475 | GPL1261 |
|
Adult murine spleen, Mature B cells (CD19+IgM+) #1
|
Adult murine spleen from 10-15 weeks old mice
|
Wild type C57bl/6
|
Gene expression data from LSKCD34-Flt3- cells FACS sorted from adult BM
|
| Sample_geo_accession | GSM280475
| | Sample_status | Public on Jun 29 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Apr 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were directly sorted into buffer RLT and RNA prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard AffymetrixTM; Small Sample Labelling Protocol v.2, with the exception that the second round of in vitro transcription (IVT) was performed using AffymetrixTM GeneChipTM Expression 3’amplification kit.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
| | Sample_data_processing | Expression values were calculated by RMA express using background adjustment and quantile normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Robert,,Månsson
| | Sample_contact_email | robert.mansson@ki.se
| | Sample_contact_department | Hemapotoietic stem cell biology
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Klinikgatan 26, BMC B12
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 221 84
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280475/suppl/GSM280475.CEL.gz
| | Sample_series_id | GSE11110
| | Sample_data_row_count | 45101
| | |
|
GSM280476 | GPL1261 |
|
Adult murine spleen, Mature B cells (CD19+IgM+) #2
|
Adult murine spleen from 10-15 weeks old mice
|
Wild type C57bl/6
|
Gene expression data from LSKCD34-Flt3- cells FACS sorted from adult BM
|
| Sample_geo_accession | GSM280476
| | Sample_status | Public on Jun 29 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Apr 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were directly sorted into buffer RLT and RNA prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard AffymetrixTM; Small Sample Labelling Protocol v.2, with the exception that the second round of in vitro transcription (IVT) was performed using AffymetrixTM GeneChipTM Expression 3’amplification kit.
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
| | Sample_data_processing | Expression values were calculated by RMA express using background adjustment and quantile normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Robert,,Månsson
| | Sample_contact_email | robert.mansson@ki.se
| | Sample_contact_department | Hemapotoietic stem cell biology
| | Sample_contact_institute | Lund University
| | Sample_contact_address | Klinikgatan 26, BMC B12
| | Sample_contact_city | Lund
| | Sample_contact_zip/postal_code | 221 84
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280476/suppl/GSM280476.CEL.gz
| | Sample_series_id | GSE11110
| | Sample_data_row_count | 45101
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