Search results for the GEO ID: GSE11113  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM280489 | GPL339 |
|
Ovary_Line FL1__biological rep1_technical rep1
|
Sample pools, ovaries from six animals, metestrous stage
|
mouse line selected for high fertility performance
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280489
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280489/suppl/GSM280489.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280490 | GPL339 |
|
Ovary_Line FL1_biological rep1_technical rep2
|
Sample pools, ovaries from six animals, metestrous stage
|
mouse line selected for high fertility performance
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280490
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280490/suppl/GSM280490.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280491 | GPL339 |
|
Ovary_Line FL1_biological rep2_technical rep1
|
Sample pools, ovaries from six animals, metestrous stage
|
mouse line selected for high fertility performance
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280491
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280491/suppl/GSM280491.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280492 | GPL339 |
|
Ovary_Line FL1_biological rep2_technical rep2
|
Sample pools, ovaries from six animals, metestrous stage
|
mouse line selected for high fertility performance
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280492
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280492/suppl/GSM280492.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280493 | GPL339 |
|
Ovary_Line FL1_biological rep3_technical rep1
|
Sample pools, ovaries from six animals, metestrous stage
|
mouse line selected for high fertility performance
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280493
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280493/suppl/GSM280493.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280494 | GPL339 |
|
Ovary_Line FL1_biological rep3_technical rep2
|
Sample pools, ovaries from six animals, metestrous stage
|
mouse line selected for high fertility performance
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280494
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280494/suppl/GSM280494.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280495 | GPL339 |
|
Ovary_Line FL1_biological rep4_technical rep1
|
Sample pools, ovaries from six animals, metestrous stage
|
mouse line selected for high fertility performance
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280495
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280495/suppl/GSM280495.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280496 | GPL339 |
|
Ovary_Line FL1_biological rep4_technical rep2
|
Sample pools, ovaries from six animals, metestrous stage
|
mouse line selected for high fertility performance
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280496
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280496/suppl/GSM280496.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280497 | GPL339 |
|
Ovary_Line FL1_biological rep5_technical rep1
|
Sample pools, ovaries from six animals, metestrous stage
|
mouse line selected for high fertility performance
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280497
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280497/suppl/GSM280497.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280498 | GPL339 |
|
Ovary_Line FL1_biological rep5_technical rep2
|
Sample pools, ovaries from six animals, metestrous stage
|
mouse line selected for high fertility performance
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280498
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280498/suppl/GSM280498.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280499 | GPL339 |
|
Ovary_Line FL1_biological rep6_technical rep1
|
Sample pools, ovaries from six animals, metestrous stage
|
mouse line selected for high fertility performance
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280499
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280499/suppl/GSM280499.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280500 | GPL339 |
|
Ovary_Line FL1_biological rep6_technical rep2
|
Sample pools, ovaries from six animals, metestrous stage
|
mouse line selected for high fertility performance
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280500
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280500/suppl/GSM280500.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280501 | GPL339 |
|
Ovary_Line DUKsi_biological rep1_technical rep1
|
Sample pools, ovaries from six animals, metestrous stage
|
non-selected control line
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280501
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280501/suppl/GSM280501.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280502 | GPL339 |
|
Ovary_Line DUKsi_biological rep1_technical rep2
|
Sample pools, ovaries from six animals, metestrous stage
|
non-selected control line
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280502
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280502/suppl/GSM280502.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280503 | GPL339 |
|
Ovary_Line DUKsi_biological rep2_technical rep1
|
Sample pools, ovaries from six animals, metestrous stage
|
non-selected control line
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280503
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280503/suppl/GSM280503.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280504 | GPL339 |
|
Ovary_Line DUKsi_biological rep2_technical rep2
|
Sample pools, ovaries from six animals, metestrous stage
|
non-selected control line
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280504
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280504/suppl/GSM280504.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280505 | GPL339 |
|
Ovary_Line DUKsi_biological rep3_technical rep1
|
Sample pools, ovaries from six animals, metestrous stage
|
non-selected control line
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280505
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280505/suppl/GSM280505.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280506 | GPL339 |
|
Ovary_Line DUKsi_biological rep3_technical rep2
|
Sample pools, ovaries from six animals, metestrous stage
|
non-selected control line
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280506
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280506/suppl/GSM280506.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280507 | GPL339 |
|
Ovary_Line DUKsi_biological rep4_technical rep1
|
Sample pools, ovaries from six animals, metestrous stage
|
non-selected control line
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280507
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280507/suppl/GSM280507.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280508 | GPL339 |
|
Ovary_Line DUKsi_biological rep4_technical rep2
|
Sample pools, ovaries from six animals, metestrous stage
|
non-selected control line
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280508
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280508/suppl/GSM280508.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280509 | GPL339 |
|
Ovary_Line DUKsi_biological rep5_technical rep1
|
Sample pools, ovaries from six animals, metestrous stage
|
non-selected control line
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280509
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280509/suppl/GSM280509.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280510 | GPL339 |
|
Ovary_Line DUKsi_biological rep5_technical rep2
|
Sample pools, ovaries from six animals, metestrous stage
|
non-selected control line
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280510
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280510/suppl/GSM280510.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280511 | GPL339 |
|
Ovary_Line DUKsi_biological rep6_technical rep1
|
Sample pools, ovaries from six animals, metestrous stage
|
non-selected control line
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280511
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280511/suppl/GSM280511.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
GSM280512 | GPL339 |
|
Ovary_Line DUKsi_biological rep6_technical rep2
|
Sample pools, ovaries from six animals, metestrous stage
|
non-selected control line
about six week old females at metestrous stage
|
Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage to identify genes that are involved in increasing the ovulation number.
|
| Sample_geo_accession | GSM280512
| | Sample_status | Public on Sep 22 2008
| | Sample_submission_date | Apr 09 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 = Line FL1 was selected for an index trait, combining litter size (LS0) and litter weight (LW0) at birth in primiparous females (Index (I) | 1.6 x LS0 + LW0)) to generation 130
| | Sample_growth_protocol_ch1 | Mice were kept in Makrolon-cages of 30 x 12.5 x 12.5 cm with free access to pellet concentrate and water. The light regime was LD 12:12, the room temperature was standardized to 22.5 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared with the RNeasey Mini Kit with simultaneous removal of genomic DNA with RNAse free DNAse (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
| | Sample_hyb_protocol | The fragmented cRNA was hybridized overnight (45°C) to the Affymetrix GeneChip MOE 430A mouse expression arrays. Each sample pool was hybridized to two arrays to reduce the technical variation. Arrays were then washed using the GeneChip Fluidics Station (Affymetrix) according to the manufacturer’s protocol and stained by R-Phycoerythrin Streptavidin (Molecular Probes). This was followed by an antibody amplification procedure using a biotinylated anti-streptavidin antibody (Vector laboratories) and goat IgG (Sigma).
| | Sample_scan_protocol | Scanning was carried out with 3µm resolution, 488nm excitation and 570nm emission wavelengths employing the GeneArray Scanner 2500 (Hewlett Packard)
| | Sample_data_processing | For calculation of signal intensity the Affymetrix Microarray Suite 5.0 Software (MAS5) was used. All arrays were normalized by scaling to reach a common target intensity.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Jens,,Vanselow
| | Sample_contact_email | vanselow@fbn-dummerstorf.de
| | Sample_contact_phone | +49 38208 68706
| | Sample_contact_fax | +49 38208 68702
| | Sample_contact_laboratory | Genregulation
| | Sample_contact_department | Molekularbiologie
| | Sample_contact_institute | Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
| | Sample_contact_address | Wilhelm-Stahl-Allee 2
| | Sample_contact_city | Dummerstorf
| | Sample_contact_zip/postal_code | 18196
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280512/suppl/GSM280512.CEL.gz
| | Sample_series_id | GSE11113
| | Sample_data_row_count | 22690
| | |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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