Search results for the GEO ID: GSE11115  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM280379 | GPL96 |
|
U2OS-ER beta cells treated with TNF, sample 1
|
human U2OS cells
|
human osteosarcoma U2OS cells, stably transfected with ER-beta
|
U2OS-ER beta cells treated with TNF, sample 1.
|
| Sample_geo_accession | GSM280379
| | Sample_status | Public on Apr 12 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Apr 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | the stable cell was generated in Dr. D.C. Leitman's laboratory
| | Sample_treatment_protocol_ch1 | Expression of ER in the U2OS-ERβ cells was induced with doxycycline. The cells were treated in the absence or presence of 10 nM E2 for 18 h followed by incubation with TNF-{alpha} for 1 h.
| | Sample_growth_protocol_ch1 | Human U2OS osteosarcoma cell lines stably expressing a tetracycline-regulated ERβ (U2OS-ERβ) cDNA were grown in 5% stripped FBS with 50 µg/ml hygromycin B and 500 µg/ml zeocin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_hyb_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_scan_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_data_processing | MAS5 was used to generate the CHP files.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dale,,Leitman
| | Sample_contact_department | Ob/Gyn
| | Sample_contact_institute | University of California, San Francisco
| | Sample_contact_address | 513 Parnassus Ave.
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://leitmanlab.com/index.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280379/suppl/GSM280379.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280379/suppl/GSM280379.CHP.gz
| | Sample_series_id | GSE11115
| | Sample_data_row_count | 22283
| | |
|
GSM280380 | GPL96 |
|
U2OS-ER beta cells treated with TNF, sample 2
|
human osteosarcoma U2OS cells, stably transfected with ER-beta
|
human osteosarcoma U2OS cells, stably transfected with ER-beta, treated with TNF
|
U2OS-ER beta cells treated with TNF, sample 1.
|
| Sample_geo_accession | GSM280380
| | Sample_status | Public on Apr 12 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Apr 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | the stable cell line was generated in Dr. D.C. Leitman's laboratory
| | Sample_treatment_protocol_ch1 | Expression of ER in the U2OS-ERβ cells was induced with doxycycline. The cells were treated in the absence or presence of 10 nM E2 for 18 h followed by incubation with TNF-{alpha} for 1 h.
| | Sample_growth_protocol_ch1 | Human U2OS osteosarcoma cell lines stably expressing a tetracycline-regulated ERβ (U2OS-ERβ) cDNA were grown in 5% stripped FBS with 50 µg/ml hygromycin B and 500 µg/ml zeocin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_hyb_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_scan_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_data_processing | MAS5 was used to generate the CHP files.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dale,,Leitman
| | Sample_contact_department | Ob/Gyn
| | Sample_contact_institute | University of California, San Francisco
| | Sample_contact_address | 513 Parnassus Ave.
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://leitmanlab.com/index.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280380/suppl/GSM280380.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280380/suppl/GSM280380.CHP.gz
| | Sample_series_id | GSE11115
| | Sample_data_row_count | 22283
| | |
|
GSM280381 | GPL96 |
|
U2OS-ER beta cells treated with TNF, sample 3
|
human osteosarcoma U2OS cells, stably transfected with ER-beta
|
human osteosarcoma U2OS cells, stably transfected with ER-beta, treated with TNF
|
U2OS-ER beta cells treated with TNF, sample 3
|
| Sample_geo_accession | GSM280381
| | Sample_status | Public on Apr 12 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Apr 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | the stable cell line was generated in Dr. D.C. Leitman's laboratory
| | Sample_treatment_protocol_ch1 | Expression of ER in the U2OS-ERß cells was induced with doxycycline. The cells were treated in the absence or presence of 10 nM E2 for 18 h followed by incubation with TNF-{alpha} for 1 h.
| | Sample_growth_protocol_ch1 | Human U2OS osteosarcoma cell lines stably expressing a tetracycline-regulated ERß (U2OS-ERß) cDNA were grown in 5% stripped FBS with 50 µg/ml hygromycin B and 500 µg/ml zeocin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_hyb_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_scan_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_data_processing | MAS5
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dale,,Leitman
| | Sample_contact_department | Ob/Gyn
| | Sample_contact_institute | University of California, San Francisco
| | Sample_contact_address | 513 Parnassus Ave.
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://leitmanlab.com/index.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280381/suppl/GSM280381.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280381/suppl/GSM280381.CHP.gz
| | Sample_series_id | GSE11115
| | Sample_data_row_count | 22283
| | |
|
GSM280382 | GPL96 |
|
U2OS-ER beta cells treated with TNF, sample 4
|
human osteosarcoma U2OS cells, stably transfected with ER-beta
|
human osteosarcoma U2OS cells, stably transfected with ER-beta, treated with TNF
|
U2OS-ER beta cells treated with TNF, sample 4
|
| Sample_geo_accession | GSM280382
| | Sample_status | Public on Apr 12 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Apr 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | the stable cell line was generated in Dr. D.C. Leitman's laboratory
| | Sample_treatment_protocol_ch1 | Expression of ER in the U2OS-ERß cells was induced with doxycycline. The cells were treated in the absence or presence of 10 nM E2 for 18 h followed by incubation with TNF-{alpha} for 1 h.
| | Sample_growth_protocol_ch1 | Human U2OS osteosarcoma cell lines stably expressing a tetracycline-regulated ERß (U2OS-ERß) cDNA were grown in 5% stripped FBS with 50 µg/ml hygromycin B and 500 µg/ml zeocin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_hyb_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_scan_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_data_processing | MAS5
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dale,,Leitman
| | Sample_contact_department | Ob/Gyn
| | Sample_contact_institute | University of California, San Francisco
| | Sample_contact_address | 513 Parnassus Ave.
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://leitmanlab.com/index.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280382/suppl/GSM280382.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280382/suppl/GSM280382.CHP.gz
| | Sample_series_id | GSE11115
| | Sample_data_row_count | 22283
| | |
|
GSM280383 | GPL96 |
|
U2OS-ER beta cells treated with E2 and TNF, sample 1
|
human osteosarcoma U2OS cells, stably transfected with ER-beta
|
human osteosarcoma U2OS cells, stably transfected with ER-beta, treated with E2 and TNF, sample 1
|
U2OS-ER beta cells treated with E2 and TNF, sample 1
|
| Sample_geo_accession | GSM280383
| | Sample_status | Public on Apr 12 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Apr 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | the stable cell line was generated in Dr. D.C. Leitman's laboratory
| | Sample_treatment_protocol_ch1 | Expression of ER in the U2OS-ERß cells was induced with doxycycline. The cells were treated in the absence or presence of 10 nM E2 for 18 h followed by incubation with TNF-{alpha} for 1 h.
| | Sample_growth_protocol_ch1 | Human U2OS osteosarcoma cell lines stably expressing a tetracycline-regulated ERß (U2OS-ERß) cDNA were grown in 5% stripped FBS with 50 µg/ml hygromycin B and 500 µg/ml zeocin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_hyb_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_scan_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_data_processing | MAS5
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dale,,Leitman
| | Sample_contact_department | Ob/Gyn
| | Sample_contact_institute | University of California, San Francisco
| | Sample_contact_address | 513 Parnassus Ave.
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://leitmanlab.com/index.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280383/suppl/GSM280383.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280383/suppl/GSM280383.CHP.gz
| | Sample_series_id | GSE11115
| | Sample_data_row_count | 22283
| | |
|
GSM280384 | GPL96 |
|
U2OS-ER beta cells treated with E2 and TNF, sample 2
|
human osteosarcoma U2OS cells, stably transfected with ER-beta
|
human osteosarcoma U2OS cells, stably transfected with ER-beta, treated with E2 and TNF
|
U2OS-ER beta cells treated with E2 and TNF, sample 2
|
| Sample_geo_accession | GSM280384
| | Sample_status | Public on Apr 12 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Apr 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | the stable cell line was generated in Dr. D.C. Leitman's laboratory
| | Sample_treatment_protocol_ch1 | Expression of ER in the U2OS-ERß cells was induced with doxycycline. The cells were treated in the absence or presence of 10 nM E2 for 18 h followed by incubation with TNF-{alpha} for 1 h.
| | Sample_growth_protocol_ch1 | Human U2OS osteosarcoma cell lines stably expressing a tetracycline-regulated ERß (U2OS-ERß) cDNA were grown in 5% stripped FBS with 50 µg/ml hygromycin B and 500 µg/ml zeocin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_hyb_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_scan_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_data_processing | MAS5
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dale,,Leitman
| | Sample_contact_department | Ob/Gyn
| | Sample_contact_institute | University of California, San Francisco
| | Sample_contact_address | 513 Parnassus Ave.
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://leitmanlab.com/index.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280384/suppl/GSM280384.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280384/suppl/GSM280384.CHP.gz
| | Sample_series_id | GSE11115
| | Sample_data_row_count | 22283
| | |
|
GSM280385 | GPL96 |
|
U2OS-ER beta cells treated with E2 and TNF, sample 3
|
human osteosarcoma U2OS cells, stably transfected with ER-beta
|
human osteosarcoma U2OS cells, stably transfected with ER-beta, treated with E2 and TNF
|
U2OS-ER beta cells treated with E2 and TNF, sample 3
|
| Sample_geo_accession | GSM280385
| | Sample_status | Public on Apr 12 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Apr 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | the stable cell line was generated in Dr. D.C. Leitman's laboratory
| | Sample_treatment_protocol_ch1 | Expression of ER in the U2OS-ERß cells was induced with doxycycline. The cells were treated in the absence or presence of 10 nM E2 for 18 h followed by incubation with TNF-{alpha} for 1 h.
| | Sample_growth_protocol_ch1 | Human U2OS osteosarcoma cell lines stably expressing a tetracycline-regulated ERß (U2OS-ERß) cDNA were grown in 5% stripped FBS with 50 µg/ml hygromycin B and 500 µg/ml zeocin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_hyb_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_scan_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_data_processing | MAS5
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dale,,Leitman
| | Sample_contact_department | Ob/Gyn
| | Sample_contact_institute | University of California, San Francisco
| | Sample_contact_address | 513 Parnassus Ave.
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://leitmanlab.com/index.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280385/suppl/GSM280385.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280385/suppl/GSM280385.CHP.gz
| | Sample_series_id | GSE11115
| | Sample_data_row_count | 22283
| | |
|
GSM280386 | GPL96 |
|
U2OS-ER beta cells treated with E2 and TNF, sample 4
|
human osteosarcoma U2OS cells, stably transfected with ER-beta
|
human osteosarcoma U2OS cells, stably transfected with ER-beta, treated with E2 and TNF
|
U2OS-ER beta cells treated with E2 and TNF, sample 4
|
| Sample_geo_accession | GSM280386
| | Sample_status | Public on Apr 12 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Apr 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | the stable cell line was generated in Dr. D.C. Leitman's laboratory
| | Sample_treatment_protocol_ch1 | Expression of ER in the U2OS-ERß cells was induced with doxycycline. The cells were treated in the absence or presence of 10 nM E2 for 18 h followed by incubation with TNF-{alpha} for 1 h.
| | Sample_growth_protocol_ch1 | Human U2OS osteosarcoma cell lines stably expressing a tetracycline-regulated ERß (U2OS-ERß) cDNA were grown in 5% stripped FBS with 50 µg/ml hygromycin B and 500 µg/ml zeocin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_hyb_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_scan_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_data_processing | MAS5
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dale,,Leitman
| | Sample_contact_department | Ob/Gyn
| | Sample_contact_institute | University of California, San Francisco
| | Sample_contact_address | 513 Parnassus Ave.
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://leitmanlab.com/index.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280386/suppl/GSM280386.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280386/suppl/GSM280386.CHP.gz
| | Sample_series_id | GSE11115
| | Sample_data_row_count | 22283
| | |
|
GSM280387 | GPL96 |
|
U2OS-ER beta cells untreated, sample 1
|
human osteosarcoma U2OS cells, stably transfected with ER-beta
|
human osteosarcoma U2OS cells, stably transfected with ER-beta, untreated
|
U2OS-ER beta cells, untreated
|
| Sample_geo_accession | GSM280387
| | Sample_status | Public on Apr 12 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Apr 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | the stable cell line was generated in Dr. D.C. Leitman's laboratory
| | Sample_treatment_protocol_ch1 | Expression of ER in the U2OS-ERß cells was induced with doxycycline. The cells were treated in the absence or presence of 10 nM E2 for 18 h followed by incubation with TNF-{alpha} for 1 h.
| | Sample_growth_protocol_ch1 | Human U2OS osteosarcoma cell lines stably expressing a tetracycline-regulated ERß (U2OS-ERß) cDNA were grown in 5% stripped FBS with 50 µg/ml hygromycin B and 500 µg/ml zeocin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_hyb_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_scan_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_data_processing | MAS5
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dale,,Leitman
| | Sample_contact_department | Ob/Gyn
| | Sample_contact_institute | University of California, San Francisco
| | Sample_contact_address | 513 Parnassus Ave.
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://leitmanlab.com/index.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280387/suppl/GSM280387.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280387/suppl/GSM280387.CHP.gz
| | Sample_series_id | GSE11115
| | Sample_data_row_count | 22283
| | |
|
GSM280388 | GPL96 |
|
U2OS-ER beta cells untreated, sample 2
|
human osteosarcoma U2OS cells, stably transfected with ER-beta
|
human osteosarcoma U2OS cells, stably transfected with ER-beta, untreated
|
U2OS-ER beta cells, untreated
|
| Sample_geo_accession | GSM280388
| | Sample_status | Public on Apr 12 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Apr 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | the stable cell line was generated in Dr. D.C. Leitman's laboratory
| | Sample_treatment_protocol_ch1 | Expression of ER in the U2OS-ERß cells was induced with doxycycline. The cells were treated in the absence or presence of 10 nM E2 for 18 h followed by incubation with TNF-{alpha} for 1 h.
| | Sample_growth_protocol_ch1 | Human U2OS osteosarcoma cell lines stably expressing a tetracycline-regulated ERß (U2OS-ERß) cDNA were grown in 5% stripped FBS with 50 µg/ml hygromycin B and 500 µg/ml zeocin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_hyb_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_scan_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_data_processing | MAS5
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dale,,Leitman
| | Sample_contact_department | Ob/Gyn
| | Sample_contact_institute | University of California, San Francisco
| | Sample_contact_address | 513 Parnassus Ave.
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://leitmanlab.com/index.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280388/suppl/GSM280388.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280388/suppl/GSM280388.CHP.gz
| | Sample_series_id | GSE11115
| | Sample_data_row_count | 22283
| | |
|
GSM280389 | GPL96 |
|
U2OS-ER beta cells untreated, sample 3
|
human osteosarcoma U2OS cells, stably transfected with ER-beta
|
human osteosarcoma U2OS cells, stably transfected with ER-beta, untreated
|
U2OS-ER beta cells, untreated
|
| Sample_geo_accession | GSM280389
| | Sample_status | Public on Apr 12 2008
| | Sample_submission_date | Apr 08 2008
| | Sample_last_update_date | Apr 11 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | the stable cell line was generated in Dr. D.C. Leitman's laboratory
| | Sample_treatment_protocol_ch1 | Expression of ER in the U2OS-ERß cells was induced with doxycycline. The cells were treated in the absence or presence of 10 nM E2 for 18 h followed by incubation with TNF-{alpha} for 1 h.
| | Sample_growth_protocol_ch1 | Human U2OS osteosarcoma cell lines stably expressing a tetracycline-regulated ERß (U2OS-ERß) cDNA were grown in 5% stripped FBS with 50 µg/ml hygromycin B and 500 µg/ml zeocin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_label_protocol_ch1 | Standard protocol as recommended by Affymetrix.
| | Sample_hyb_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_scan_protocol | Standard protocol as recommended by Affymetrix.
| | Sample_data_processing | MAS5
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dale,,Leitman
| | Sample_contact_department | Ob/Gyn
| | Sample_contact_institute | University of California, San Francisco
| | Sample_contact_address | 513 Parnassus Ave.
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://leitmanlab.com/index.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280389/suppl/GSM280389.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280389/suppl/GSM280389.CHP.gz
| | Sample_series_id | GSE11115
| | Sample_data_row_count | 22283
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