Search results for the GEO ID: GSE11118 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM280586 | GPL570 |
|
sample 1A zero 32--2, technical rep1
|
Jurkat T-cells were harvest at 0
|
Jurkat T-cells were harvest at 0
|
Mitogen stimulated Jurkat T-cells were harvest at 0, 30 min, 1hr, 2hr, 4hr, and 6hr and RNA was collected.
|
Sample_geo_accession | GSM280586
| Sample_status | Public on Dec 04 2009
| Sample_submission_date | Apr 09 2008
| Sample_last_update_date | Dec 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Jurkat Cells were treated with P/I for 45 min.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Human promoter microarray from NimbleGen Systems, Inc., was co-hybridized with either antibody precipitated and total imput control or an IgG-precipitated sample with the total imput control.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II Biotin-enhanced protocol from 1 ug total RNA (Message Amp II Biotin-Enhanced Technical Manual, 2006, Ambion).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus 2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 Laser Scanner.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Jung,S,Byun
| Sample_contact_email | byunjs@mail.nih.gov
| Sample_contact_phone | 3014352890
| Sample_contact_department | Laboratory of Receptor Biology and Gene Expression
| Sample_contact_institute | NCI/NIH
| Sample_contact_address | 41 Library Drive
| Sample_contact_city | Building 41/Rm D305
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280586/suppl/GSM280586.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280586/suppl/GSM280586.CHP.gz
| Sample_series_id | GSE11118
| Sample_data_row_count | 54675
| |
|
GSM280587 | GPL570 |
|
sample 1B zero 32--2, technical rep2
|
Jurkat T-cells were harvest at 0
|
Jurkat T-cells were harvest at 0
|
Mitogen stimulated Jurkat T-cells were harvest at 0, 30 min, 1hr, 2hr, 4hr, and 6hr and RNA was collected.
|
Sample_geo_accession | GSM280587
| Sample_status | Public on Dec 04 2009
| Sample_submission_date | Apr 09 2008
| Sample_last_update_date | Dec 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Jurkat Cells were treated with P/I for 45 min.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Human promoter microarray from NimbleGen Systems, Inc., was co-hybridized with either antibody precipitated and total imput control or an IgG-precipitated sample with the total imput control.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II Biotin-enhanced protocol from 1 ug total RNA (Message Amp II Biotin-Enhanced Technical Manual, 2006, Ambion).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus 2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 Laser Scanner.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Jung,S,Byun
| Sample_contact_email | byunjs@mail.nih.gov
| Sample_contact_phone | 3014352890
| Sample_contact_department | Laboratory of Receptor Biology and Gene Expression
| Sample_contact_institute | NCI/NIH
| Sample_contact_address | 41 Library Drive
| Sample_contact_city | Building 41/Rm D305
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280587/suppl/GSM280587.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280587/suppl/GSM280587.CHP.gz
| Sample_series_id | GSE11118
| Sample_data_row_count | 54675
| |
|
GSM280588 | GPL570 |
|
sample 2A 30min 32--3, technical rep1
|
Jurkat T-cells were harvest at 30 min
|
Jurkat T-cells were harvest at 30 min
|
Mitogen stimulated Jurkat T-cells were harvest at 0, 30 min, 1hr, 2hr, 4hr, and 6hr and RNA was collected.
|
Sample_geo_accession | GSM280588
| Sample_status | Public on Dec 04 2009
| Sample_submission_date | Apr 09 2008
| Sample_last_update_date | Dec 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Jurkat Cells were treated with P/I for 45 min.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Human promoter microarray from NimbleGen Systems, Inc., was co-hybridized with either antibody precipitated and total imput control or an IgG-precipitated sample with the total imput control.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II Biotin-enhanced protocol from 1 ug total RNA (Message Amp II Biotin-Enhanced Technical Manual, 2006, Ambion).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus 2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 Laser Scanner.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Jung,S,Byun
| Sample_contact_email | byunjs@mail.nih.gov
| Sample_contact_phone | 3014352890
| Sample_contact_department | Laboratory of Receptor Biology and Gene Expression
| Sample_contact_institute | NCI/NIH
| Sample_contact_address | 41 Library Drive
| Sample_contact_city | Building 41/Rm D305
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280588/suppl/GSM280588.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280588/suppl/GSM280588.CHP.gz
| Sample_series_id | GSE11118
| Sample_data_row_count | 54675
| |
|
GSM280589 | GPL570 |
|
sample 2B 30min 32--3, technical rep2
|
Jurkat T-cells were harvest at 30 min
|
Jurkat T-cells were harvest at 30 min
|
Mitogen stimulated Jurkat T-cells were harvest at 0, 30 min, 1hr, 2hr, 4hr, and 6hr and RNA was collected.
|
Sample_geo_accession | GSM280589
| Sample_status | Public on Dec 04 2009
| Sample_submission_date | Apr 09 2008
| Sample_last_update_date | Dec 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Jurkat Cells were treated with P/I for 45 min.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Human promoter microarray from NimbleGen Systems, Inc., was co-hybridized with either antibody precipitated and total imput control or an IgG-precipitated sample with the total imput control.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II Biotin-enhanced protocol from 1 ug total RNA (Message Amp II Biotin-Enhanced Technical Manual, 2006, Ambion).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus 2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 Laser Scanner.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Jung,S,Byun
| Sample_contact_email | byunjs@mail.nih.gov
| Sample_contact_phone | 3014352890
| Sample_contact_department | Laboratory of Receptor Biology and Gene Expression
| Sample_contact_institute | NCI/NIH
| Sample_contact_address | 41 Library Drive
| Sample_contact_city | Building 41/Rm D305
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280589/suppl/GSM280589.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280589/suppl/GSM280589.CHP.gz
| Sample_series_id | GSE11118
| Sample_data_row_count | 54675
| |
|
GSM280590 | GPL570 |
|
sample 3A 1hr 32--4, technical rep1
|
Jurkat T-cells were harvest at 1hr
|
Jurkat T-cells were harvest at 1hr
|
Mitogen stimulated Jurkat T-cells were harvest at 0, 30 min, 1hr, 2hr, 4hr, and 6hr and RNA was collected.
|
Sample_geo_accession | GSM280590
| Sample_status | Public on Dec 04 2009
| Sample_submission_date | Apr 09 2008
| Sample_last_update_date | Dec 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Jurkat Cells were treated with P/I for 45 min.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Human promoter microarray from NimbleGen Systems, Inc., was co-hybridized with either antibody precipitated and total imput control or an IgG-precipitated sample with the total imput control.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II Biotin-enhanced protocol from 1 ug total RNA (Message Amp II Biotin-Enhanced Technical Manual, 2006, Ambion).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus 2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 Laser Scanner.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Jung,S,Byun
| Sample_contact_email | byunjs@mail.nih.gov
| Sample_contact_phone | 3014352890
| Sample_contact_department | Laboratory of Receptor Biology and Gene Expression
| Sample_contact_institute | NCI/NIH
| Sample_contact_address | 41 Library Drive
| Sample_contact_city | Building 41/Rm D305
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280590/suppl/GSM280590.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280590/suppl/GSM280590.CHP.gz
| Sample_series_id | GSE11118
| Sample_data_row_count | 54675
| |
|
GSM280591 | GPL570 |
|
sample 3b 1hr 32--4, technical rep2
|
Jurkat T-cells were harvest at 1hr
|
Jurkat T-cells were harvest at 1hr
|
Mitogen stimulated Jurkat T-cells were harvest at 0, 30 min, 1hr, 2hr, 4hr, and 6hr and RNA was collected.
|
Sample_geo_accession | GSM280591
| Sample_status | Public on Dec 04 2009
| Sample_submission_date | Apr 09 2008
| Sample_last_update_date | Dec 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Jurkat Cells were treated with P/I for 45 min.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Human promoter microarray from NimbleGen Systems, Inc., was co-hybridized with either antibody precipitated and total imput control or an IgG-precipitated sample with the total imput control.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II Biotin-enhanced protocol from 1 ug total RNA (Message Amp II Biotin-Enhanced Technical Manual, 2006, Ambion).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus 2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 Laser Scanner.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Jung,S,Byun
| Sample_contact_email | byunjs@mail.nih.gov
| Sample_contact_phone | 3014352890
| Sample_contact_department | Laboratory of Receptor Biology and Gene Expression
| Sample_contact_institute | NCI/NIH
| Sample_contact_address | 41 Library Drive
| Sample_contact_city | Building 41/Rm D305
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280591/suppl/GSM280591.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280591/suppl/GSM280591.CHP.gz
| Sample_series_id | GSE11118
| Sample_data_row_count | 54675
| |
|
GSM280592 | GPL570 |
|
sample 7a zero 33--1, biological rep2, technical rep1
|
Jurkat T-cells were harvest at 0
|
Jurkat T-cells were harvest at 0
|
Mitogen stimulated Jurkat T-cells were harvest at 0, 30 min, 1hr, 2hr, 4hr, and 6hr and RNA was collected.
|
Sample_geo_accession | GSM280592
| Sample_status | Public on Dec 04 2009
| Sample_submission_date | Apr 09 2008
| Sample_last_update_date | Dec 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Jurkat Cells were treated with P/I for 45 min.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Human promoter microarray from NimbleGen Systems, Inc., was co-hybridized with either antibody precipitated and total imput control or an IgG-precipitated sample with the total imput control.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II Biotin-enhanced protocol from 1 ug total RNA (Message Amp II Biotin-Enhanced Technical Manual, 2006, Ambion).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus 2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 Laser Scanner.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Jung,S,Byun
| Sample_contact_email | byunjs@mail.nih.gov
| Sample_contact_phone | 3014352890
| Sample_contact_department | Laboratory of Receptor Biology and Gene Expression
| Sample_contact_institute | NCI/NIH
| Sample_contact_address | 41 Library Drive
| Sample_contact_city | Building 41/Rm D305
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280592/suppl/GSM280592.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280592/suppl/GSM280592.CHP.gz
| Sample_series_id | GSE11118
| Sample_data_row_count | 54675
| |
|
GSM280594 | GPL570 |
|
sample 8a 30min 33--3, biological rep2, technical rep1
|
Jurkat T-cells were harvest at 30 min
|
Jurkat T-cells were harvest at 30 min
|
Mitogen stimulated Jurkat T-cells were harvest at 0, 30 min, 1hr, 2hr, 4hr, and 6hr and RNA was collected.
|
Sample_geo_accession | GSM280594
| Sample_status | Public on Dec 04 2009
| Sample_submission_date | Apr 09 2008
| Sample_last_update_date | Dec 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Jurkat Cells were treated with P/I for 45 min.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Human promoter microarray from NimbleGen Systems, Inc., was co-hybridized with either antibody precipitated and total imput control or an IgG-precipitated sample with the total imput control.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II Biotin-enhanced protocol from 1 ug total RNA (Message Amp II Biotin-Enhanced Technical Manual, 2006, Ambion).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus 2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 Laser Scanner.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Jung,S,Byun
| Sample_contact_email | byunjs@mail.nih.gov
| Sample_contact_phone | 3014352890
| Sample_contact_department | Laboratory of Receptor Biology and Gene Expression
| Sample_contact_institute | NCI/NIH
| Sample_contact_address | 41 Library Drive
| Sample_contact_city | Building 41/Rm D305
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280594/suppl/GSM280594.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280594/suppl/GSM280594.CHP.gz
| Sample_series_id | GSE11118
| Sample_data_row_count | 54675
| |
|
GSM280595 | GPL570 |
|
sample 8b 30min 33--3, biological rep2, technical rep2
|
Jurkat T-cells were harvest at 30 min
|
Jurkat T-cells were harvest at 30 min
|
Mitogen stimulated Jurkat T-cells were harvest at 0, 30 min, 1hr, 2hr, 4hr, and 6hr and RNA was collected.
|
Sample_geo_accession | GSM280595
| Sample_status | Public on Dec 04 2009
| Sample_submission_date | Apr 09 2008
| Sample_last_update_date | Dec 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Jurkat Cells were treated with P/I for 45 min.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Human promoter microarray from NimbleGen Systems, Inc., was co-hybridized with either antibody precipitated and total imput control or an IgG-precipitated sample with the total imput control.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II Biotin-enhanced protocol from 1 ug total RNA (Message Amp II Biotin-Enhanced Technical Manual, 2006, Ambion).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus 2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 Laser Scanner.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Jung,S,Byun
| Sample_contact_email | byunjs@mail.nih.gov
| Sample_contact_phone | 3014352890
| Sample_contact_department | Laboratory of Receptor Biology and Gene Expression
| Sample_contact_institute | NCI/NIH
| Sample_contact_address | 41 Library Drive
| Sample_contact_city | Building 41/Rm D305
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280595/suppl/GSM280595.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280595/suppl/GSM280595.CHP.gz
| Sample_series_id | GSE11118
| Sample_data_row_count | 54675
| |
|
GSM280596 | GPL570 |
|
sample 9a 1hr 33--4, biological rep2, technical rep1
|
Jurkat T-cells were harvest at 1hr
|
Jurkat T-cells were harvest at 1hr
|
Mitogen stimulated Jurkat T-cells were harvest at 0, 30 min, 1hr, 2hr, 4hr, and 6hr and RNA was collected.
|
Sample_geo_accession | GSM280596
| Sample_status | Public on Dec 04 2009
| Sample_submission_date | Apr 09 2008
| Sample_last_update_date | Dec 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Jurkat Cells were treated with P/I for 45 min.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Human promoter microarray from NimbleGen Systems, Inc., was co-hybridized with either antibody precipitated and total imput control or an IgG-precipitated sample with the total imput control.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II Biotin-enhanced protocol from 1 ug total RNA (Message Amp II Biotin-Enhanced Technical Manual, 2006, Ambion).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus 2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 Laser Scanner.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Jung,S,Byun
| Sample_contact_email | byunjs@mail.nih.gov
| Sample_contact_phone | 3014352890
| Sample_contact_department | Laboratory of Receptor Biology and Gene Expression
| Sample_contact_institute | NCI/NIH
| Sample_contact_address | 41 Library Drive
| Sample_contact_city | Building 41/Rm D305
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280596/suppl/GSM280596.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280596/suppl/GSM280596.CHP.gz
| Sample_series_id | GSE11118
| Sample_data_row_count | 54675
| |
|
GSM280597 | GPL570 |
|
sample 9b 1hr 33--4, biological rep2, technical rep2
|
Jurkat T-cells were harvest at 1hr
|
Jurkat T-cells were harvest at 1hr
|
Mitogen stimulated Jurkat T-cells were harvest at 0, 30 min, 1hr, 2hr, 4hr, and 6hr and RNA was collected.
|
Sample_geo_accession | GSM280597
| Sample_status | Public on Dec 04 2009
| Sample_submission_date | Apr 09 2008
| Sample_last_update_date | Dec 04 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | Jurkat Cells were treated with P/I for 45 min.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Human promoter microarray from NimbleGen Systems, Inc., was co-hybridized with either antibody precipitated and total imput control or an IgG-precipitated sample with the total imput control.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II Biotin-enhanced protocol from 1 ug total RNA (Message Amp II Biotin-Enhanced Technical Manual, 2006, Ambion).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus 2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 Laser Scanner.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Jung,S,Byun
| Sample_contact_email | byunjs@mail.nih.gov
| Sample_contact_phone | 3014352890
| Sample_contact_department | Laboratory of Receptor Biology and Gene Expression
| Sample_contact_institute | NCI/NIH
| Sample_contact_address | 41 Library Drive
| Sample_contact_city | Building 41/Rm D305
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280597/suppl/GSM280597.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280597/suppl/GSM280597.CHP.gz
| Sample_series_id | GSE11118
| Sample_data_row_count | 54675
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