Result

Search results for the GEO ID: GSE11133

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM280797

GPL1355

Ovary cultured control rep1

4-day rat ovary cultured 2 days

ovaries dissected from 4-day old Sprague-Dawley rats and cleaned of ovarian bursa.

mRNA from 4-day old rat ovaries cultured two days as controls with no treatment.

Sample_geo_accession	GSM280797
Sample_status	Public on Apr 11 2008
Sample_submission_date	Apr 10 2008
Sample_last_update_date	Apr 10 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Ovaries dissected from four-day old female rat pups were maintained in a whole organ culture system on floating filters (0.4 um Millicell-CM; Millipore Corp., Billerica, MA) in 0.5 ml DMEM-Ham’s F-12 medium (1:1, vol/vol; Life Technologies, Inc., Rockville, Maryland) containing 0.1% BSA (Sigma, St. Louis, MO), 0.1% albumax (Life Technologies, Inc., Rockville, Maryland), 200 ng/ml insulin (rh Insulin; Sigma, St. Louis, 10 MO), 0.05 mg/ml L-ascorbic acid (Sigma, St. Louis, MO) and 27.5 ug/ml transferrin (Sigma, St. Louis, MO) in a four-well culture plate (Nunc plate; Applied Scientific, South San Francisco, CA). Medium was supplemented with final concentration 5 ug/ml gentamicin, 3.25 ug/ml streptomycin, and 3.25 units/ml penicillin to prevent bacterial contamination. Ovaries were treated with no factor (control). Each sample contained 6-10 pooled ovaries, and no two ovaries from the same animal were placed into the same group.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturers instructions (Sigma, St. Louis, MO).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix procedures
Sample_hyb_protocol	standard Affymetrix procedure
Sample_scan_protocol	standard Affymetrix procedure
Sample_data_processing = Auto-scale ON (1-1000). Scaling	all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using MAS5 preprocessing and a scaling factor of 125.
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280797/suppl/GSM280797.CEL.gz
Sample_series_id	GSE11133
Sample_data_row_count	31099

GSM280798

GPL1355

Ovary cultured control rep2

4-day rat ovary cultured 2 days

ovaries dissected from 4-day old Sprague-Dawley rats and cleaned of ovarian bursa.

mRNA from 4-day old rat ovaries cultured two days as controls with no treatment.

Sample_geo_accession	GSM280798
Sample_status	Public on Apr 11 2008
Sample_submission_date	Apr 10 2008
Sample_last_update_date	Apr 10 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Ovaries dissected from four-day old female rat pups were maintained in a whole organ culture system on floating filters (0.4 um Millicell-CM; Millipore Corp., Billerica, MA) in 0.5 ml DMEM-Ham’s F-12 medium (1:1, vol/vol; Life Technologies, Inc., Rockville, Maryland) containing 0.1% BSA (Sigma, St. Louis, MO), 0.1% albumax (Life Technologies, Inc., Rockville, Maryland), 200 ng/ml insulin (rh Insulin; Sigma, St. Louis, 10 MO), 0.05 mg/ml L-ascorbic acid (Sigma, St. Louis, MO) and 27.5 ug/ml transferrin (Sigma, St. Louis, MO) in a four-well culture plate (Nunc plate; Applied Scientific, South San Francisco, CA). Medium was supplemented with final concentration 5 ug/ml gentamicin, 3.25 ug/ml streptomycin, and 3.25 units/ml penicillin to prevent bacterial contamination. Ovaries were treated with no factor (control). Each sample contained 6-10 pooled ovaries, and no two ovaries from the same animal were placed into the same group.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturers instructions (Sigma, St. Louis, MO).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix procedures
Sample_hyb_protocol	standard Affymetrix procedure
Sample_scan_protocol	standard Affymetrix procedure
Sample_data_processing = Auto-scale ON (1-1000). Scaling	all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using MAS5 preprocessing and a scaling factor of 125.
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280798/suppl/GSM280798.CEL.gz
Sample_series_id	GSE11133
Sample_data_row_count	31099

GSM280799

GPL1355

Ovary cultured GDNF rep1

4-day rat ovary cultured 2 days

ovaries dissected from 4-day old Sprague-Dawley rats and cleaned of ovarian bursa.

mRNA from 4-day old rat ovaries cultured two days with GDNF treatment.

Sample_geo_accession	GSM280799
Sample_status	Public on Apr 11 2008
Sample_submission_date	Apr 10 2008
Sample_last_update_date	Apr 10 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Ovaries dissected from four-day old female rat pups were maintained in a whole organ culture system on floating filters (0.4 um Millicell-CM; Millipore Corp., Billerica, MA) in 0.5 ml DMEM-Ham’s F-12 medium (1:1, vol/vol; Life Technologies, Inc., Rockville, Maryland) containing 0.1% BSA (Sigma, St. Louis, MO), 0.1% albumax (Life Technologies, Inc., Rockville, Maryland), 200 ng/ml insulin (rh Insulin; Sigma, St. Louis, 10 MO), 0.05 mg/ml L-ascorbic acid (Sigma, St. Louis, MO) and 27.5 ug/ml transferrin (Sigma, St. Louis, MO) in a four-well culture plate (Nunc plate; Applied Scientific, South San Francisco, CA). Medium was supplemented with final concentration 5 ug/ml gentamicin, 3.25 ug/ml streptomycin, and 3.25 units/ml penicillin to prevent bacterial contamination. Ovaries were treated with no factor (control). Each sample contained 6-10 pooled ovaries, and no two ovaries from the same animal were placed into the same group.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturers instructions (Sigma, St. Louis, MO).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix procedures
Sample_hyb_protocol	standard Affymetrix procedure
Sample_scan_protocol	standard Affymetrix procedure
Sample_data_processing = Auto-scale ON (1-1000). Scaling	all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using MAS5 preprocessing and a scaling factor of 125.
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280799/suppl/GSM280799.CEL.gz
Sample_series_id	GSE11133
Sample_data_row_count	31099

GSM280800

GPL1355

Ovary cultured GDNF rep2

4-day rat ovary cultured 2 days

ovaries dissected from 4-day old Sprague-Dawley rats and cleaned of ovarian bursa.

mRNA from 4-day old rat ovaries cultured two days with GDNF treatment.

Sample_geo_accession	GSM280800
Sample_status	Public on Apr 11 2008
Sample_submission_date	Apr 10 2008
Sample_last_update_date	Apr 10 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Ovaries dissected from four-day old female rat pups were maintained in a whole organ culture system on floating filters (0.4 um Millicell-CM; Millipore Corp., Billerica, MA) in 0.5 ml DMEM-Ham’s F-12 medium (1:1, vol/vol; Life Technologies, Inc., Rockville, Maryland) containing 0.1% BSA (Sigma, St. Louis, MO), 0.1% albumax (Life Technologies, Inc., Rockville, Maryland), 200 ng/ml insulin (rh Insulin; Sigma, St. Louis, 10 MO), 0.05 mg/ml L-ascorbic acid (Sigma, St. Louis, MO) and 27.5 ug/ml transferrin (Sigma, St. Louis, MO) in a four-well culture plate (Nunc plate; Applied Scientific, South San Francisco, CA). Medium was supplemented with final concentration 5 ug/ml gentamicin, 3.25 ug/ml streptomycin, and 3.25 units/ml penicillin to prevent bacterial contamination. Ovaries were treated with no factor (control). Each sample contained 6-10 pooled ovaries, and no two ovaries from the same animal were placed into the same group.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Ovaries with the same treatment were pooled to make each RNA sample. RNA was extracted using the Trizol reagent according to manufacturers instructions (Sigma, St. Louis, MO).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix procedures
Sample_hyb_protocol	standard Affymetrix procedure
Sample_scan_protocol	standard Affymetrix procedure
Sample_data_processing = Auto-scale ON (1-1000). Scaling	all probe sets. The microarray image data were converted to numerical data with GeneChip Operating Software using MAS5 preprocessing and a scaling factor of 125.
Sample_platform_id	GPL1355
Sample_contact_name	Michael,K,Skinner
Sample_contact_email	skinner@mail.wsu.edu
Sample_contact_department	SBS
Sample_contact_institute	WSU
Sample_contact_address	Abelson 507
Sample_contact_city	Pullman
Sample_contact_state	WA
Sample_contact_zip/postal_code	99163
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM280nnn/GSM280800/suppl/GSM280800.CEL.gz
Sample_series_id	GSE11133
Sample_data_row_count	31099

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