Search results for the GEO ID: GSE11161 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM281573 | GPL1261 |
|
postnatal day 2 bladder cells EGFP positive, EYFP negative, rep 1
|
postnatal day 2 bladder cells EGFP positive, EYFP negative (detrusor muscle of bladder)
|
gene reported: SMAA/EYFP SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: male
developmental stage: postnatal day 2
theiler stage: 27
somite count: ND
developmental landmark: EGFP positive, EYFP negative
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9424
|
Sample_geo_accession | GSM281573
| Sample_status | Public on Apr 17 2008
| Sample_submission_date | Apr 14 2008
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | pool size: N/A
| Sample_treatment_protocol_ch1 | Pooled sample: no
| Sample_treatment_protocol_ch1 | Dissection Method: Bladder microdissected based on SMGA/EGFP and SMAA/EYFP expression and digested using lysis enzymes.
| Sample_treatment_protocol_ch1 | SMAA/EYFP mice were time mated to SMGA/EGFP transgenic mice. Postnatal mice were euthanized by decapitation and tissues were micro-dissected and digested with Blendzymes 1 & 4 and subjected to FACS sorting. The separated cell populations were rapidly stored at -80ºC until RNA was extracted for gene expression analysis.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen: RNeasy
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| Sample_hyb_protocol | Affymetrix standard protcol
| Sample_hyb_protocol | Amount labeled target hybridization to array: 10µg
| Sample_scan_protocol | Affymetrix standard protcol
| Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| Sample_platform_id | GPL1261
| Sample_contact_name | GUDMAP,,Developers
| Sample_contact_email | gudmap-editors@gudmap.org
| Sample_contact_phone | +44 131 3322471
| Sample_contact_laboratory | GUDMAP Database Group
| Sample_contact_institute | MRC Human Genetics Unit
| Sample_contact_address | Crewe Road
| Sample_contact_city | Edinburgh
| Sample_contact_zip/postal_code | EH4 2XU
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM281nnn/GSM281573/suppl/GSM281573.CEL.gz
| Sample_series_id | GSE11161
| Sample_data_row_count | 45101
| |
|
GSM281574 | GPL1261 |
|
postnatal day 2 bladder cells EGFP positive, EYFP positive, rep 1
|
postnatal day 2 bladder cells EGFP positive, EYFP positive (detrusor muscle of bladder)
|
gene reported: SMAA/EYFP SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: male
developmental stage: postnatal day 2
theiler stage: 27
somite count: ND
developmental landmark: EGFP positive, EYFP positive
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9425
|
Sample_geo_accession | GSM281574
| Sample_status | Public on Apr 17 2008
| Sample_submission_date | Apr 14 2008
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | pool size: N/A
| Sample_treatment_protocol_ch1 | Pooled sample: no
| Sample_treatment_protocol_ch1 | Dissection Method: Bladder microdissected based on SMGA/EGFP and SMAA/EYFP expression and digested using lysis enzymes.
| Sample_treatment_protocol_ch1 | SMAA/EYFP mice were time mated to SMGA/EGFP transgenic mice. Postnatal mice were euthanized by decapitation and tissues were micro-dissected and digested with Blendzymes 1 & 4 and subjected to FACS sorting. The separated cell populations were rapidly stored at -80ºC until RNA was extracted for gene expression analysis.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen: RNeasy
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| Sample_hyb_protocol | Affymetrix standard protcol
| Sample_hyb_protocol | Amount labeled target hybridization to array: 10µg
| Sample_scan_protocol | Affymetrix standard protcol
| Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| Sample_platform_id | GPL1261
| Sample_contact_name | GUDMAP,,Developers
| Sample_contact_email | gudmap-editors@gudmap.org
| Sample_contact_phone | +44 131 3322471
| Sample_contact_laboratory | GUDMAP Database Group
| Sample_contact_institute | MRC Human Genetics Unit
| Sample_contact_address | Crewe Road
| Sample_contact_city | Edinburgh
| Sample_contact_zip/postal_code | EH4 2XU
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM281nnn/GSM281574/suppl/GSM281574.CEL.gz
| Sample_series_id | GSE11161
| Sample_data_row_count | 45101
| |
|
GSM281575 | GPL1261 |
|
postnatal day 2 bladder cells EGFP positive, EYFP negative, rep 2
|
postnatal day 2 bladder cells EGFP positive, EYFP negative (detrusor muscle of bladder)
|
gene reported: SMAA/EYFP SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: male
developmental stage: postnatal day 2
theiler stage: 27
somite count: ND
developmental landmark: EGFP positive, EYFP negative
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9426
|
Sample_geo_accession | GSM281575
| Sample_status | Public on Apr 17 2008
| Sample_submission_date | Apr 14 2008
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | pool size: N/A
| Sample_treatment_protocol_ch1 | Pooled sample: no
| Sample_treatment_protocol_ch1 | Dissection Method: Bladder microdissected based on SMGA/EGFP and SMAA/EYFP expression and digested using lysis enzymes.
| Sample_treatment_protocol_ch1 | SMAA/EYFP mice were time mated to SMGA/EGFP transgenic mice. Postnatal mice were euthanized by decapitation and tissues were micro-dissected and digested with Blendzymes 1 & 4 and subjected to FACS sorting. The separated cell populations were rapidly stored at -80ºC until RNA was extracted for gene expression analysis.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen: RNeasy
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| Sample_hyb_protocol | Affymetrix standard protcol
| Sample_hyb_protocol | Amount labeled target hybridization to array: 10µg
| Sample_scan_protocol | Affymetrix standard protcol
| Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| Sample_platform_id | GPL1261
| Sample_contact_name | GUDMAP,,Developers
| Sample_contact_email | gudmap-editors@gudmap.org
| Sample_contact_phone | +44 131 3322471
| Sample_contact_laboratory | GUDMAP Database Group
| Sample_contact_institute | MRC Human Genetics Unit
| Sample_contact_address | Crewe Road
| Sample_contact_city | Edinburgh
| Sample_contact_zip/postal_code | EH4 2XU
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM281nnn/GSM281575/suppl/GSM281575.CEL.gz
| Sample_series_id | GSE11161
| Sample_data_row_count | 45101
| |
|
GSM281576 | GPL1261 |
|
postnatal day 2 bladder cells EGFP positive, EYFP positive, rep 2
|
postnatal day 2 bladder cells EGFP positive, EYFP positive (detrusor muscle of bladder)
|
gene reported: SMAA/EYFP SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: male
developmental stage: postnatal day 2
theiler stage: 27
somite count: ND
developmental landmark: EGFP positive, EYFP positive
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9427
|
Sample_geo_accession | GSM281576
| Sample_status | Public on Apr 17 2008
| Sample_submission_date | Apr 14 2008
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | pool size: N/A
| Sample_treatment_protocol_ch1 | Pooled sample: no
| Sample_treatment_protocol_ch1 | Dissection Method: Bladder microdissected based on SMGA/EGFP and SMAA/EYFP expression and digested using lysis enzymes.
| Sample_treatment_protocol_ch1 | SMAA/EYFP mice were time mated to SMGA/EGFP transgenic mice. Postnatal mice were euthanized by decapitation and tissues were micro-dissected and digested with Blendzymes 1 & 4 and subjected to FACS sorting. The separated cell populations were rapidly stored at -80ºC until RNA was extracted for gene expression analysis.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen: RNeasy
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| Sample_hyb_protocol | Affymetrix standard protcol
| Sample_hyb_protocol | Amount labeled target hybridization to array: 10µg
| Sample_scan_protocol | Affymetrix standard protcol
| Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| Sample_platform_id | GPL1261
| Sample_contact_name | GUDMAP,,Developers
| Sample_contact_email | gudmap-editors@gudmap.org
| Sample_contact_phone | +44 131 3322471
| Sample_contact_laboratory | GUDMAP Database Group
| Sample_contact_institute | MRC Human Genetics Unit
| Sample_contact_address | Crewe Road
| Sample_contact_city | Edinburgh
| Sample_contact_zip/postal_code | EH4 2XU
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM281nnn/GSM281576/suppl/GSM281576.CEL.gz
| Sample_series_id | GSE11161
| Sample_data_row_count | 45101
| |
|
GSM281577 | GPL1261 |
|
postnatal day 2 bladder cells EGFP positive, EYFP negative, rep 3
|
postnatal day 2 bladder cells EGFP positive, EYFP negative (detrusor muscle of bladder)
|
gene reported: SMAA/EYFP SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: male
developmental stage: postnatal day 2
theiler stage: 27
somite count: ND
developmental landmark: EGFP positive, EYFP negative
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9428
|
Sample_geo_accession | GSM281577
| Sample_status | Public on Apr 17 2008
| Sample_submission_date | Apr 14 2008
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | pool size: N/A
| Sample_treatment_protocol_ch1 | Pooled sample: no
| Sample_treatment_protocol_ch1 | Dissection Method: Bladder microdissected based on SMGA/EGFP and SMAA/EYFP expression and digested using lysis enzymes.
| Sample_treatment_protocol_ch1 | SMAA/EYFP mice were time mated to SMGA/EGFP transgenic mice. Postnatal mice were euthanized by decapitation and tissues were micro-dissected and digested with Blendzymes 1 & 4 and subjected to FACS sorting. The separated cell populations were rapidly stored at -80ºC until RNA was extracted for gene expression analysis.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen: RNeasy
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| Sample_hyb_protocol | Affymetrix standard protcol
| Sample_hyb_protocol | Amount labeled target hybridization to array: 10µg
| Sample_scan_protocol | Affymetrix standard protcol
| Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| Sample_platform_id | GPL1261
| Sample_contact_name | GUDMAP,,Developers
| Sample_contact_email | gudmap-editors@gudmap.org
| Sample_contact_phone | +44 131 3322471
| Sample_contact_laboratory | GUDMAP Database Group
| Sample_contact_institute | MRC Human Genetics Unit
| Sample_contact_address | Crewe Road
| Sample_contact_city | Edinburgh
| Sample_contact_zip/postal_code | EH4 2XU
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM281nnn/GSM281577/suppl/GSM281577.CEL.gz
| Sample_series_id | GSE11161
| Sample_data_row_count | 45101
| |
|
GSM281578 | GPL1261 |
|
postnatal day 2 bladder cells EGFP positive, EYFP positive, rep 3
|
postnatal day 2 bladder cells EGFP positive, EYFP positive (detrusor muscle of bladder)
|
gene reported: SMAA/EYFP SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: male
developmental stage: postnatal day 2
theiler stage: 27
somite count: ND
developmental landmark: EGFP positive, EYFP positive
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9429
|
Sample_geo_accession | GSM281578
| Sample_status | Public on Apr 17 2008
| Sample_submission_date | Apr 14 2008
| Sample_last_update_date | Jan 03 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | pool size: N/A
| Sample_treatment_protocol_ch1 | Pooled sample: no
| Sample_treatment_protocol_ch1 | Dissection Method: Bladder microdissected based on SMGA/EGFP and SMAA/EYFP expression and digested using lysis enzymes.
| Sample_treatment_protocol_ch1 | SMAA/EYFP mice were time mated to SMGA/EGFP transgenic mice. Postnatal mice were euthanized by decapitation and tissues were micro-dissected and digested with Blendzymes 1 & 4 and subjected to FACS sorting. The separated cell populations were rapidly stored at -80ºC until RNA was extracted for gene expression analysis.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen: RNeasy
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| Sample_hyb_protocol | Affymetrix standard protcol
| Sample_hyb_protocol | Amount labeled target hybridization to array: 10µg
| Sample_scan_protocol | Affymetrix standard protcol
| Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| Sample_platform_id | GPL1261
| Sample_contact_name | GUDMAP,,Developers
| Sample_contact_email | gudmap-editors@gudmap.org
| Sample_contact_phone | +44 131 3322471
| Sample_contact_laboratory | GUDMAP Database Group
| Sample_contact_institute | MRC Human Genetics Unit
| Sample_contact_address | Crewe Road
| Sample_contact_city | Edinburgh
| Sample_contact_zip/postal_code | EH4 2XU
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM281nnn/GSM281578/suppl/GSM281578.CEL.gz
| Sample_series_id | GSE11161
| Sample_data_row_count | 45101
| |
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