Search results for the GEO ID: GSE11221  |
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(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM282804 | GPL1261 |
|
2801_Detrusor
|
bladder detrusor
|
gene reported: SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: unknown
developmental stage: postnatal day 1
theiler stage: 27
somite count: ND
developmental landmark:
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9430
|
| Sample_geo_accession | GSM282804
| | Sample_status | Public on Apr 22 2008
| | Sample_submission_date | Apr 21 2008
| | Sample_last_update_date | Jan 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: N/A
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Tissue layer isolated and dissected using laser capture microscopy for dissection.
| | Sample_treatment_protocol_ch1 | Bladders were isolated from newborn SMGA/EGFP transgenic mice, embedded in OCT, frozen and sectioned (8 microns). Detrusor, stroma and urothelium were isolated using laser capture microscopy. The caps were frozen on dry ice and stored at -80 ºC until RNA was extracted for gene expression analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen: RNeasy
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282804/suppl/GSM282804_2801_Detrusor.CEL.gz
| | Sample_series_id | GSE11221
| | Sample_data_row_count | 45101
| | |
|
GSM282805 | GPL1261 |
|
1848_Detrusor
|
bladder detrusor
|
gene reported: SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: unknown
developmental stage: postnatal day 1
theiler stage: 27
somite count: ND
developmental landmark:
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9431
|
| Sample_geo_accession | GSM282805
| | Sample_status | Public on Apr 22 2008
| | Sample_submission_date | Apr 21 2008
| | Sample_last_update_date | Jan 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: N/A
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Tissue layer isolated and dissected using laser capture microscopy for dissection.
| | Sample_treatment_protocol_ch1 | Bladders were isolated from newborn SMGA/EGFP transgenic mice, embedded in OCT, frozen and sectioned (8 microns). Detrusor, stroma and urothelium were isolated using laser capture microscopy. The caps were frozen on dry ice and stored at -80 ºC until RNA was extracted for gene expression analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen: RNeasy
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282805/suppl/GSM282805_1848_Detrusor.CEL.gz
| | Sample_series_id | GSE11221
| | Sample_data_row_count | 45101
| | |
|
GSM282806 | GPL1261 |
|
2715_Detrusor
|
bladder detrusor
|
gene reported: SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: unknown
developmental stage: postnatal day 2
theiler stage: 27
somite count: ND
developmental landmark:
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9432
|
| Sample_geo_accession | GSM282806
| | Sample_status | Public on Apr 22 2008
| | Sample_submission_date | Apr 21 2008
| | Sample_last_update_date | Jan 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: N/A
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Tissue layer isolated and dissected using laser capture microscopy for dissection.
| | Sample_treatment_protocol_ch1 | Bladders were isolated from newborn SMGA/EGFP transgenic mice, embedded in OCT, frozen and sectioned (8 microns). Detrusor, stroma and urothelium were isolated using laser capture microscopy. The caps were frozen on dry ice and stored at -80 ºC until RNA was extracted for gene expression analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen: RNeasy
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282806/suppl/GSM282806_2715_Detrusor.CEL.gz
| | Sample_series_id | GSE11221
| | Sample_data_row_count | 45101
| | |
|
GSM282807 | GPL1261 |
|
2299_Urothelium
|
bladder urothelium
|
gene reported: SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: unknown
developmental stage: postnatal day 2
theiler stage: 27
somite count: ND
developmental landmark:
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9433
|
| Sample_geo_accession | GSM282807
| | Sample_status | Public on Apr 22 2008
| | Sample_submission_date | Apr 21 2008
| | Sample_last_update_date | Jan 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: N/A
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Tissue layer isolated and dissected using laser capture microscopy for dissection.
| | Sample_treatment_protocol_ch1 | Bladders were isolated from newborn SMGA/EGFP transgenic mice, embedded in OCT, frozen and sectioned (8 microns). Detrusor, stroma and urothelium were isolated using laser capture microscopy. The caps were frozen on dry ice and stored at -80 ºC until RNA was extracted for gene expression analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen: RNeasy
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282807/suppl/GSM282807_2299_Urothelium.CEL.gz
| | Sample_series_id | GSE11221
| | Sample_data_row_count | 45101
| | |
|
GSM282808 | GPL1261 |
|
2951_Urothelium
|
bladder urothelium
|
gene reported: SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: unknown
developmental stage: postnatal day 1
theiler stage: 27
somite count: ND
developmental landmark:
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9434
|
| Sample_geo_accession | GSM282808
| | Sample_status | Public on Apr 22 2008
| | Sample_submission_date | Apr 21 2008
| | Sample_last_update_date | Jan 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: N/A
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Tissue layer isolated and dissected using laser capture microscopy for dissection.
| | Sample_treatment_protocol_ch1 | Bladders were isolated from newborn SMGA/EGFP transgenic mice, embedded in OCT, frozen and sectioned (8 microns). Detrusor, stroma and urothelium were isolated using laser capture microscopy. The caps were frozen on dry ice and stored at -80 ºC until RNA was extracted for gene expression analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen: RNeasy
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282808/suppl/GSM282808_2951_Urothelium.CEL.gz
| | Sample_series_id | GSE11221
| | Sample_data_row_count | 45101
| | |
|
GSM282809 | GPL1261 |
|
2291_Urothelium
|
bladder urothelium
|
gene reported: SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: unknown
developmental stage: postnatal day 2
theiler stage: 27
somite count: ND
developmental landmark:
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9435
|
| Sample_geo_accession | GSM282809
| | Sample_status | Public on Apr 22 2008
| | Sample_submission_date | Apr 21 2008
| | Sample_last_update_date | Jan 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: N/A
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Tissue layer isolated and dissected using laser capture microscopy for dissection.
| | Sample_treatment_protocol_ch1 | Bladders were isolated from newborn SMGA/EGFP transgenic mice, embedded in OCT, frozen and sectioned (8 microns). Detrusor, stroma and urothelium were isolated using laser capture microscopy. The caps were frozen on dry ice and stored at -80 ºC until RNA was extracted for gene expression analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen: RNeasy
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282809/suppl/GSM282809_2291_Urothelium.CEL.gz
| | Sample_series_id | GSE11221
| | Sample_data_row_count | 45101
| | |
|
GSM282810 | GPL1261 |
|
2833_Stroma
|
bladder stroma
|
gene reported: SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: unknown
developmental stage: postnatal day 1
theiler stage: 27
somite count: ND
developmental landmark:
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9436
|
| Sample_geo_accession | GSM282810
| | Sample_status | Public on Apr 22 2008
| | Sample_submission_date | Apr 21 2008
| | Sample_last_update_date | Jan 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: N/A
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Tissue layer isolated and dissected using laser capture microscopy for dissection.
| | Sample_treatment_protocol_ch1 | Bladders were isolated from newborn SMGA/EGFP transgenic mice, embedded in OCT, frozen and sectioned (8 microns). Detrusor, stroma and urothelium were isolated using laser capture microscopy. The caps were frozen on dry ice and stored at -80 ºC until RNA was extracted for gene expression analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen: RNeasy
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282810/suppl/GSM282810_2833_Stroma.CEL.gz
| | Sample_series_id | GSE11221
| | Sample_data_row_count | 45101
| | |
|
GSM282811 | GPL1261 |
|
2865_Stroma
|
bladder stroma
|
gene reported: SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: unknown
developmental stage: postnatal day 2
theiler stage: 27
somite count: ND
developmental landmark:
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9437
|
| Sample_geo_accession | GSM282811
| | Sample_status | Public on Apr 22 2008
| | Sample_submission_date | Apr 21 2008
| | Sample_last_update_date | Jan 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: N/A
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Tissue layer isolated and dissected using laser capture microscopy for dissection.
| | Sample_treatment_protocol_ch1 | Bladders were isolated from newborn SMGA/EGFP transgenic mice, embedded in OCT, frozen and sectioned (8 microns). Detrusor, stroma and urothelium were isolated using laser capture microscopy. The caps were frozen on dry ice and stored at -80 ºC until RNA was extracted for gene expression analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen: RNeasy
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282811/suppl/GSM282811_2865_Stroma.CEL.gz
| | Sample_series_id | GSE11221
| | Sample_data_row_count | 45101
| | |
|
GSM282812 | GPL1261 |
|
2295_Stroma
|
bladder stroma
|
gene reported: SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: unknown
developmental stage: postnatal day 2
theiler stage: 27
somite count: ND
developmental landmark:
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9438
|
| Sample_geo_accession | GSM282812
| | Sample_status | Public on Apr 22 2008
| | Sample_submission_date | Apr 21 2008
| | Sample_last_update_date | Jan 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: N/A
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Tissue layer isolated and dissected using laser capture microscopy for dissection.
| | Sample_treatment_protocol_ch1 | Bladders were isolated from newborn SMGA/EGFP transgenic mice, embedded in OCT, frozen and sectioned (8 microns). Detrusor, stroma and urothelium were isolated using laser capture microscopy. The caps were frozen on dry ice and stored at -80 ºC until RNA was extracted for gene expression analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen: RNeasy
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282812/suppl/GSM282812_2295_Stroma.CEL.gz
| | Sample_series_id | GSE11221
| | Sample_data_row_count | 45101
| | |
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