Result

Search results for the GEO ID: GSE11232

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM283125

GPL1261

FLK1_1

endothelial layer of renal vein & endothelial layer of renal artery

gene reported: Tg(TIE2GFP)287Sato / MGI:3056937 strain: CD-1 sex: unknown developmental stage: dpc15.5 theiler stage: 23 somite count: NA developmental landmark: NA

>> Amplification protocol << Target Amplified manufacturer/kit: TargetAmp 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies) Target Amplified protocol: Potter protocols Rounds of amplification: 2 URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:9439

Sample_geo_accession	GSM283125
Sample_status	Public on Jun 18 2008
Sample_submission_date	Apr 22 2008
Sample_last_update_date	Dec 31 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: approx 5-10 E15.5 kidneys
Sample_treatment_protocol_ch1	Pooled sample: Yes
Sample_treatment_protocol_ch1	Dissection Method: Trypsinization & FACS
Sample_treatment_protocol_ch1	Tie2 transgenic mice are time-mated. Pregnant Tie2 transgenic mice are euthanized by standard carbon dioxide asphyxiation. All fetuses are killed by decapitation with a scalpel. Fetal kidneys are dissected from fetuses and placed in ice-cold PBS. Embryonic kidneys are incubated in the presence of 300µl of trypsin for 5 minutes at 37 ºC. Kidneys are then dissociated by titurating in the presence of 600µl of ice-cold 10%FBS/PBS. The dissociated cells from the kidneys are pelleted at 5000 rpm, 4 ºC for 5 minutes. The media is aspirated and the cell pellet is resuspended in 200µl of ice-cold 2%FBS/PBS. Filter cells through 70 micron mesh filter and proceed with FACS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Potter protocol: 'RNA purification'
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-X-X-NHS (EPICENTRE Biotechnologies)
Sample_hyb_protocol	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 5ug
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
Sample_platform_id	GPL1261
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM283nnn/GSM283125/suppl/GSM283125.CEL.gz
Sample_series_id	GSE11232
Sample_data_row_count	45101

GSM283126

GPL1261

FLK1_2

endothelial layer of renal vein & endothelial layer of renal artery

gene reported: Tg(TIE2GFP)287Sato / MGI:3056937 strain: CD-1 sex: unknown developmental stage: dpc15.5 theiler stage: 23 somite count: NA developmental landmark: NA

Sample_geo_accession	GSM283126
Sample_status	Public on Jun 18 2008
Sample_submission_date	Apr 22 2008
Sample_last_update_date	Dec 31 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: approx 5-10 E15.5 kidneys
Sample_treatment_protocol_ch1	Pooled sample: Yes
Sample_treatment_protocol_ch1	Dissection Method: Trypsinization & FACS
Sample_treatment_protocol_ch1	Tie2 transgenic mice are time-mated. Pregnant Tie2 transgenic mice are euthanized by standard carbon dioxide asphyxiation. All fetuses are killed by decapitation with a scalpel. Fetal kidneys are dissected from fetuses and placed in ice-cold PBS. Embryonic kidneys are incubated in the presence of 300µl of trypsin for 5 minutes at 37 ºC. Kidneys are then dissociated by titurating in the presence of 600µl of ice-cold 10%FBS/PBS. The dissociated cells from the kidneys are pelleted at 5000 rpm, 4 ºC for 5 minutes. The media is aspirated and the cell pellet is resuspended in 200µl of ice-cold 2%FBS/PBS. Filter cells through 70 micron mesh filter and proceed with FACS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Potter protocol: 'RNA purification'
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-X-X-NHS (EPICENTRE Biotechnologies)
Sample_hyb_protocol	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 5ug
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
Sample_platform_id	GPL1261
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM283nnn/GSM283126/suppl/GSM283126.CEL.gz
Sample_series_id	GSE11232
Sample_data_row_count	45101

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