Search results for the GEO ID: GSE11266  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM284754 | GPL96 |
|
BGAL ETOH 1
|
BGAL, EtOH
|
Cell line: MCF7
Infection: BGAL
Treatment: Vehicle
|
SS283784
|
| Sample_geo_accession | GSM284754
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284754/suppl/GSM284754.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284755 | GPL96 |
|
WT ETOH 1
|
PGC1 WT, EtOH
|
Cell line: MCF7
Infection: PGC1 WT
Treatment: Vehicle
|
SS283785
|
| Sample_geo_accession | GSM284755
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284755/suppl/GSM284755.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284756 | GPL96 |
|
2x9 ETOH 1
|
PGC1 2x9, EtOH
|
Cell line: MCF7
Infection: PGC1 2x9
Treatment: Vehicle
|
SS283786
|
| Sample_geo_accession | GSM284756
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284756/suppl/GSM284756.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284757 | GPL96 |
|
L2L3 ETOH 1
|
PGC1 L2L3, EtOH
|
Cell line: MCF7
Infection: PGC1 L2L3
Treatment: Vehicle
|
SS283787
|
| Sample_geo_accession | GSM284757
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284757/suppl/GSM284757.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284758 | GPL96 |
|
BGAL E2 1
|
BGAL, E2
|
Cell line: MCF7
Infection: BGAL
Treatment: E2 10 nM
|
SS283788
|
| Sample_geo_accession | GSM284758
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284758/suppl/GSM284758.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284759 | GPL96 |
|
WT E2 1
|
PGC1 WT, E2
|
Cell line: MCF7
Infection: PGC1 WT
Treatment: E2 10 nM
|
SS283789
|
| Sample_geo_accession | GSM284759
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284759/suppl/GSM284759.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284760 | GPL96 |
|
2x9 E2 1
|
PGC1 2x9, E2
|
Cell line: MCF7
Infection: PGC1 2x9
Treatment: E2 10 nM
|
SS283790
|
| Sample_geo_accession | GSM284760
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284760/suppl/GSM284760.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284761 | GPL96 |
|
L2L3 E2 1
|
PGC1 L2L3, E2
|
Cell line: MCF7
Infection: PGC1 L2L3
Treatment: E2 10 nM
|
SS283791
|
| Sample_geo_accession | GSM284761
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284761/suppl/GSM284761.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284762 | GPL96 |
|
BGAL ETOH 3
|
BGAL, EtOH
|
Cell line: MCF7
Infection: BGAL
Treatment: Vehicle
|
SS283792
|
| Sample_geo_accession | GSM284762
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284762/suppl/GSM284762.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284763 | GPL96 |
|
WT ETOH 3
|
PGC1 WT, EtOH
|
Cell line: MCF7
Infection: PGC1 WT
Treatment: Vehicle
|
SS283793
|
| Sample_geo_accession | GSM284763
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284763/suppl/GSM284763.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284764 | GPL96 |
|
2x9 ETOH 3
|
PGC1 2x9, EtOH
|
Cell line: MCF7
Infection: PGC1 2x9
Treatment: Vehicle
|
SS283794
|
| Sample_geo_accession | GSM284764
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284764/suppl/GSM284764.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284765 | GPL96 |
|
L2L3 ETOH 3
|
PGC1 L2L3, EtOH
|
Cell line: MCF7
Infection: PGC1 L2L3
Treatment: Vehicle
|
SS283795
|
| Sample_geo_accession | GSM284765
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284765/suppl/GSM284765.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284766 | GPL96 |
|
BGAL E2 3
|
BGAL, E2
|
Cell line: MCF7
Infection: BGAL
Treatment: E2 10 nM
|
SS283796
|
| Sample_geo_accession | GSM284766
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284766/suppl/GSM284766.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284767 | GPL96 |
|
2x9 E2 3
|
PGC1 2x9, E2
|
Cell line: MCF7
Infection: PGC1 2x9
Treatment: E2 10 nM
|
SS283798
|
| Sample_geo_accession | GSM284767
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284767/suppl/GSM284767.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284768 | GPL96 |
|
L2L3 E2 3
|
PGC1 L2L3, E2
|
Cell line: MCF7
Infection: PGC1 L2L3
Treatment: E2 10 nM
|
SS283799
|
| Sample_geo_accession | GSM284768
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284768/suppl/GSM284768.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284769 | GPL96 |
|
BGAL ETOH 2
|
BGAL, EtOH
|
Cell line: MCF7
Infection: BGAL
Treatment: Vehicle
|
SS283800
|
| Sample_geo_accession | GSM284769
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284769/suppl/GSM284769.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284770 | GPL96 |
|
WT WTOH 2
|
PGC1 WT, EtOH
|
Cell line: MCF7
Infection: PGC1 WT
Treatment: Vehicle
|
SS283801
|
| Sample_geo_accession | GSM284770
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284770/suppl/GSM284770.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284771 | GPL96 |
|
2x9 ETOH 2
|
PGC1 2x9, EtOH
|
Cell line: MCF7
Infection: PGC1 2x9
Treatment: Vehicle
|
SS283802
|
| Sample_geo_accession | GSM284771
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284771/suppl/GSM284771.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284772 | GPL96 |
|
L2L3 ETOH 2
|
PGC1 L2L3, EtOH
|
Cell line: MCF7
Infection: PGC1 L2L3
Treatment: Vehicle
|
SS283803
|
| Sample_geo_accession | GSM284772
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284772/suppl/GSM284772.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284773 | GPL96 |
|
BGAL E2 2
|
BGAL,E2
|
Cell line: MCF7
Infection: BGAL
Treatment: E2 10 nM
|
SS283804
|
| Sample_geo_accession | GSM284773
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284773/suppl/GSM284773.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284774 | GPL96 |
|
WT E2 2
|
PGC1 WT,E2
|
Cell line: MCF7
Infection: PGC1 WT
Treatment: E2 10 nM
|
SS283805
|
| Sample_geo_accession | GSM284774
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284774/suppl/GSM284774.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
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GSM284775 | GPL96 |
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2x9 E2 2
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PGC1 2x9, E2
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Cell line: MCF7
Infection: PGC1 2x9
Treatment: E2 10 nM
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SS283806
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| Sample_geo_accession | GSM284775
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284775/suppl/GSM284775.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
|
GSM284776 | GPL96 |
|
L2L3 E2 2
|
PGC1 L2L3, E2
|
Cell line: MCF7
Infection: PGC1 L2L3
Treatment: E2 10 nM
|
SS283807
|
| Sample_geo_accession | GSM284776
| | Sample_status | Public on Aug 25 2008
| | Sample_submission_date | Apr 25 2008
| | Sample_last_update_date | Jul 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviral infection was performed at an MOI of 50. Twelve hours after infection cells were treated with estradiol (10nM) or vehicle and harvested 12 h thereafter.
| | Sample_growth_protocol_ch1 | For microarray experiments, MCF-7 cells were cultured in estrogen-free media for two days before adenoviral infection
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Quagen RNEasy kit following manufacturer's protocol.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | One step IVT from 10 ug totaql RNA, followed by biotin labeling of fragmented cRNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284776/suppl/GSM284776.CEL.gz
| | Sample_series_id | GSE11266
| | Sample_data_row_count | 22283
| | |
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