Result

Search results for the GEO ID: GSE11281

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM284858

GPL570

PBMCs_untreated_biorep1

PBMCs untreated donor1

donor 1 medium control untreated

PBMCs_untreated_biorep1

Sample_geo_accession	GSM284858
Sample_status	Public on Oct 01 2008
Sample_submission_date	Apr 28 2008
Sample_last_update_date	Sep 25 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Human PBMCs were purified from buffy coats of healthy blood donors and stimulated for 6h with the recombinant SAgs SEB or SEI.
Sample_growth_protocol_ch1	Purified PBMCs were cultured in RPMI containing 10% FCS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol extraction of total RNA, subsequent purification of RNA with Qiagen RNeasy Micro Kit and precipitation of RNA with Sodium acetate and ethanol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Preparation of biotinylated cRNA followed the Affymetrix standard protocol starting from 2.5 µg total RNA (GeneChip Expression Analysis Technical Manual, 2004, Affymetrix)
Sample_hyb_protocol	Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Raw data were generated with GCOS 1.4 (MAS algorithm) without scaling. Normalization was performed with GeneSpring GX 7.3.1 (Agilent) and included data transformation of signals below 10 to 10 as well as per chip and per gene median normalisation.
Sample_platform_id	GPL570
Sample_contact_name	Sandra,,Scharf
Sample_contact_department	Department of Functional Genomics
Sample_contact_institute	Ernst Moritz Arndt University Greifswald, Institute for Genetics and Functional Genomics
Sample_contact_address	F.-L.-Jahn-Str. 15a
Sample_contact_city	Greifswald
Sample_contact_zip/postal_code	17489
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284858/suppl/GSM284858.CEL.gz
Sample_series_id	GSE11281
Sample_data_row_count	54675

GSM284859

GPL570

PBMCs_SEI-treated_biorep1

PBMCs SEI-treated donor1

donor 1 treated with SEI 100pg/ml

PBMCs_SEI-treated_biorep1

Sample_geo_accession	GSM284859
Sample_status	Public on Oct 01 2008
Sample_submission_date	Apr 28 2008
Sample_last_update_date	Sep 25 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Human PBMCs were purified from buffy coats of healthy blood donors and stimulated for 6h with the recombinant SAgs SEB or SEI.
Sample_growth_protocol_ch1	Purified PBMCs were cultured in RPMI containing 10% FCS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol extraction of total RNA, subsequent purification of RNA with Qiagen RNeasy Micro Kit and precipitation of RNA with Sodium acetate and ethanol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Preparation of biotinylated cRNA followed the Affymetrix standard protocol starting from 2.5 µg total RNA (GeneChip Expression Analysis Technical Manual, 2004, Affymetrix)
Sample_hyb_protocol	Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Raw data were generated with GCOS 1.4 (MAS algorithm) without scaling. Normalization was performed with GeneSpring GX 7.3.1 (Agilent) and included data transformation of signals below 10 to 10 as well as per chip and per gene median normalisation.
Sample_platform_id	GPL570
Sample_contact_name	Sandra,,Scharf
Sample_contact_department	Department of Functional Genomics
Sample_contact_institute	Ernst Moritz Arndt University Greifswald, Institute for Genetics and Functional Genomics
Sample_contact_address	F.-L.-Jahn-Str. 15a
Sample_contact_city	Greifswald
Sample_contact_zip/postal_code	17489
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284859/suppl/GSM284859.CEL.gz
Sample_series_id	GSE11281
Sample_data_row_count	54675

GSM284860

GPL570

PBMCs_SEB-treated_biorep1

PBMCs SEB-treated donor1

donor 1 treated with SEB 100pg/ml

PBMCs_SEB-treated_biorep1

Sample_geo_accession	GSM284860
Sample_status	Public on Oct 01 2008
Sample_submission_date	Apr 28 2008
Sample_last_update_date	Sep 25 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Human PBMCs were purified from buffy coats of healthy blood donors and stimulated for 6h with the recombinant SAgs SEB or SEI.
Sample_growth_protocol_ch1	Purified PBMCs were cultured in RPMI containing 10% FCS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol extraction of total RNA, subsequent purification of RNA with Qiagen RNeasy Micro Kit and precipitation of RNA with Sodium acetate and ethanol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Preparation of biotinylated cRNA followed the Affymetrix standard protocol starting from 2.5 µg total RNA (GeneChip Expression Analysis Technical Manual, 2004, Affymetrix)
Sample_hyb_protocol	Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Raw data were generated with GCOS 1.4 (MAS algorithm) without scaling. Normalization was performed with GeneSpring GX 7.3.1 (Agilent) and included data transformation of signals below 10 to 10 as well as per chip and per gene median normalisation.
Sample_platform_id	GPL570
Sample_contact_name	Sandra,,Scharf
Sample_contact_department	Department of Functional Genomics
Sample_contact_institute	Ernst Moritz Arndt University Greifswald, Institute for Genetics and Functional Genomics
Sample_contact_address	F.-L.-Jahn-Str. 15a
Sample_contact_city	Greifswald
Sample_contact_zip/postal_code	17489
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284860/suppl/GSM284860.CEL.gz
Sample_series_id	GSE11281
Sample_data_row_count	54675

GSM284861

GPL570

PBMCs_untreated_biorep2

PBMCs untreated donor2

donor 2 medium control untreated

PBMCs_untreated_biorep2

Sample_geo_accession	GSM284861
Sample_status	Public on Oct 01 2008
Sample_submission_date	Apr 28 2008
Sample_last_update_date	Sep 25 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Human PBMCs were purified from buffy coats of healthy blood donors and stimulated for 6h with the recombinant SAgs SEB or SEI.
Sample_growth_protocol_ch1	Purified PBMCs were cultured in RPMI containing 10% FCS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol extraction of total RNA, subsequent purification of RNA with Qiagen RNeasy Micro Kit and precipitation of RNA with Sodium acetate and ethanol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Preparation of biotinylated cRNA followed the Affymetrix standard protocol starting from 2.5 µg total RNA (GeneChip Expression Analysis Technical Manual, 2004, Affymetrix)
Sample_hyb_protocol	Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Raw data were generated with GCOS 1.4 (MAS algorithm) without scaling. Normalization was performed with GeneSpring GX 7.3.1 (Agilent) and included data transformation of signals below 10 to 10 as well as per chip and per gene median normalisation.
Sample_platform_id	GPL570
Sample_contact_name	Sandra,,Scharf
Sample_contact_department	Department of Functional Genomics
Sample_contact_institute	Ernst Moritz Arndt University Greifswald, Institute for Genetics and Functional Genomics
Sample_contact_address	F.-L.-Jahn-Str. 15a
Sample_contact_city	Greifswald
Sample_contact_zip/postal_code	17489
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284861/suppl/GSM284861.CEL.gz
Sample_series_id	GSE11281
Sample_data_row_count	54675

GSM284862

GPL570

PBMCs_SEI-treated_biorep2

PBMCs SEI-treated donor2

donor 2 treated with SEI 100pg/ml

PBMCs_SEI-treated_biorep2

Sample_geo_accession	GSM284862
Sample_status	Public on Oct 01 2008
Sample_submission_date	Apr 28 2008
Sample_last_update_date	Sep 25 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Human PBMCs were purified from buffy coats of healthy blood donors and stimulated for 6h with the recombinant SAgs SEB or SEI.
Sample_growth_protocol_ch1	Purified PBMCs were cultured in RPMI containing 10% FCS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol extraction of total RNA, subsequent purification of RNA with Qiagen RNeasy Micro Kit and precipitation of RNA with Sodium acetate and ethanol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Preparation of biotinylated cRNA followed the Affymetrix standard protocol starting from 2.5 µg total RNA (GeneChip Expression Analysis Technical Manual, 2004, Affymetrix)
Sample_hyb_protocol	Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Raw data were generated with GCOS 1.4 (MAS algorithm) without scaling. Normalization was performed with GeneSpring GX 7.3.1 (Agilent) and included data transformation of signals below 10 to 10 as well as per chip and per gene median normalisation.
Sample_platform_id	GPL570
Sample_contact_name	Sandra,,Scharf
Sample_contact_department	Department of Functional Genomics
Sample_contact_institute	Ernst Moritz Arndt University Greifswald, Institute for Genetics and Functional Genomics
Sample_contact_address	F.-L.-Jahn-Str. 15a
Sample_contact_city	Greifswald
Sample_contact_zip/postal_code	17489
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284862/suppl/GSM284862.CEL.gz
Sample_series_id	GSE11281
Sample_data_row_count	54675

GSM284863

GPL570

PBMCs_SEB-treated_biorep2

PBMCs SEB-treated donor2

donor 2 treated with SEB 100pg/ml

PBMCs_SEB-treated_biorep2

Sample_geo_accession	GSM284863
Sample_status	Public on Oct 01 2008
Sample_submission_date	Apr 28 2008
Sample_last_update_date	Sep 25 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Human PBMCs were purified from buffy coats of healthy blood donors and stimulated for 6h with the recombinant SAgs SEB or SEI.
Sample_growth_protocol_ch1	Purified PBMCs were cultured in RPMI containing 10% FCS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol extraction of total RNA, subsequent purification of RNA with Qiagen RNeasy Micro Kit and precipitation of RNA with Sodium acetate and ethanol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Preparation of biotinylated cRNA followed the Affymetrix standard protocol starting from 2.5 µg total RNA (GeneChip Expression Analysis Technical Manual, 2004, Affymetrix)
Sample_hyb_protocol	Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Raw data were generated with GCOS 1.4 (MAS algorithm) without scaling. Normalization was performed with GeneSpring GX 7.3.1 (Agilent) and included data transformation of signals below 10 to 10 as well as per chip and per gene median normalisation.
Sample_platform_id	GPL570
Sample_contact_name	Sandra,,Scharf
Sample_contact_department	Department of Functional Genomics
Sample_contact_institute	Ernst Moritz Arndt University Greifswald, Institute for Genetics and Functional Genomics
Sample_contact_address	F.-L.-Jahn-Str. 15a
Sample_contact_city	Greifswald
Sample_contact_zip/postal_code	17489
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284863/suppl/GSM284863.CEL.gz
Sample_series_id	GSE11281
Sample_data_row_count	54675

GSM284864

GPL570

PBMCs_untreated_biorep3

PBMCs untreated donor3

donor 3 medium control untreated

PBMCs_untreated_biorep3

Sample_geo_accession	GSM284864
Sample_status	Public on Oct 01 2008
Sample_submission_date	Apr 28 2008
Sample_last_update_date	Sep 25 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Human PBMCs were purified from buffy coats of healthy blood donors and stimulated for 6h with the recombinant SAgs SEB or SEI.
Sample_growth_protocol_ch1	Purified PBMCs were cultured in RPMI containing 10% FCS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol extraction of total RNA, subsequent purification of RNA with Qiagen RNeasy Micro Kit and precipitation of RNA with Sodium acetate and ethanol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Preparation of biotinylated cRNA followed the Affymetrix standard protocol starting from 2.5 µg total RNA (GeneChip Expression Analysis Technical Manual, 2004, Affymetrix)
Sample_hyb_protocol	Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Raw data were generated with GCOS 1.4 (MAS algorithm) without scaling. Normalization was performed with GeneSpring GX 7.3.1 (Agilent) and included data transformation of signals below 10 to 10 as well as per chip and per gene median normalisation.
Sample_platform_id	GPL570
Sample_contact_name	Sandra,,Scharf
Sample_contact_department	Department of Functional Genomics
Sample_contact_institute	Ernst Moritz Arndt University Greifswald, Institute for Genetics and Functional Genomics
Sample_contact_address	F.-L.-Jahn-Str. 15a
Sample_contact_city	Greifswald
Sample_contact_zip/postal_code	17489
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284864/suppl/GSM284864.CEL.gz
Sample_series_id	GSE11281
Sample_data_row_count	54675

GSM284865

GPL570

PBMCs_SEI-treated_biorep3

PBMCs SEI-treated donor3

donor 3 treated with SEI 100pg/ml

PBMCs_SEI-treated_biorep3

Sample_geo_accession	GSM284865
Sample_status	Public on Oct 01 2008
Sample_submission_date	Apr 28 2008
Sample_last_update_date	Sep 25 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Human PBMCs were purified from buffy coats of healthy blood donors and stimulated for 6h with the recombinant SAgs SEB or SEI.
Sample_growth_protocol_ch1	Purified PBMCs were cultured in RPMI containing 10% FCS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol extraction of total RNA, subsequent purification of RNA with Qiagen RNeasy Micro Kit and precipitation of RNA with Sodium acetate and ethanol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Preparation of biotinylated cRNA followed the Affymetrix standard protocol starting from 2.5 µg total RNA (GeneChip Expression Analysis Technical Manual, 2004, Affymetrix)
Sample_hyb_protocol	Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Raw data were generated with GCOS 1.4 (MAS algorithm) without scaling. Normalization was performed with GeneSpring GX 7.3.1 (Agilent) and included data transformation of signals below 10 to 10 as well as per chip and per gene median normalisation.
Sample_platform_id	GPL570
Sample_contact_name	Sandra,,Scharf
Sample_contact_department	Department of Functional Genomics
Sample_contact_institute	Ernst Moritz Arndt University Greifswald, Institute for Genetics and Functional Genomics
Sample_contact_address	F.-L.-Jahn-Str. 15a
Sample_contact_city	Greifswald
Sample_contact_zip/postal_code	17489
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284865/suppl/GSM284865.CEL.gz
Sample_series_id	GSE11281
Sample_data_row_count	54675

GSM284866

GPL570

PBMCs_SEB-treated_biorep3

PBMCs SEB-treated donor3

donor 3 treated with SEB 100pg/ml

PBMCs_SEB-treated_biorep3

Sample_geo_accession	GSM284866
Sample_status	Public on Oct 01 2008
Sample_submission_date	Apr 28 2008
Sample_last_update_date	Sep 25 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Human PBMCs were purified from buffy coats of healthy blood donors and stimulated for 6h with the recombinant SAgs SEB or SEI.
Sample_growth_protocol_ch1	Purified PBMCs were cultured in RPMI containing 10% FCS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol extraction of total RNA, subsequent purification of RNA with Qiagen RNeasy Micro Kit and precipitation of RNA with Sodium acetate and ethanol.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Preparation of biotinylated cRNA followed the Affymetrix standard protocol starting from 2.5 µg total RNA (GeneChip Expression Analysis Technical Manual, 2004, Affymetrix)
Sample_hyb_protocol	Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Raw data were generated with GCOS 1.4 (MAS algorithm) without scaling. Normalization was performed with GeneSpring GX 7.3.1 (Agilent) and included data transformation of signals below 10 to 10 as well as per chip and per gene median normalisation.
Sample_platform_id	GPL570
Sample_contact_name	Sandra,,Scharf
Sample_contact_department	Department of Functional Genomics
Sample_contact_institute	Ernst Moritz Arndt University Greifswald, Institute for Genetics and Functional Genomics
Sample_contact_address	F.-L.-Jahn-Str. 15a
Sample_contact_city	Greifswald
Sample_contact_zip/postal_code	17489
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM284nnn/GSM284866/suppl/GSM284866.CEL.gz
Sample_series_id	GSE11281
Sample_data_row_count	54675

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