Search results for the GEO ID: GSE11309  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM285730 | GPL570 |
|
moDC_candida-_patient_1
|
mature moDCs from APECED patient
|
Gender: female
|
Matrix: candida neg
|
| Sample_geo_accession | GSM285730
| | Sample_status | Public on Oct 01 2008
| | Sample_submission_date | Apr 30 2008
| | Sample_last_update_date | Apr 30 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples labelled candida_pos were co-cultured with candida albicans using a cell-to-candida ratio of 1:1 (MOI of 1).
| | Sample_growth_protocol_ch1 | Peripheral blood mononuclear cells (PBMC) were isolated from 70-100 ml of EDTA blood over Ficoll–Paque gradient. PBMCs were plated at a cell density of 10×106 cells/ml onto six-well, 24-well or 96-well plates. Monocytes were allowed to adhere for 2 h at 37°C, after which non-adherent cells were removed by washing with phosphate-buffered saline (PBS). Monocytes were differentiated into moDCs in RPMI supplemented with 2 mM L-glutamine, 20 mM HEPES, 10% fetal calf serum (FCS) and containing 20 ng/ml IL-4 and 10 ng/ml GM-CSF. Fresh medium was added every two days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from DCs using the RNeasy Mini kit (Qiagen, Venlo, The Netherlands). Quantity of RNA was determined with spectrophotometer and the quality was controlled using the Agilent Bioanalyzer 2100.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total cellular RNA was treated according to the conventional Affymetrix eukaryotic RNA labeling protocols (Affymetrix, Santa Clara, CA) and then used to prepare biotinylated cRNA with the Enzo Bioarray High Yield RNA transcript labelling kit (Enzo, Life sciences, Farmingdale, NY).
| | Sample_hyb_protocol | 15 μg of biotin-labeled cRNA were fragmented according to the Affymetrix eukaryotic sample protocol and hybridized to the U133 Plus 2.0 chip (Affymetrix). Hybridization, staining and washing were performed using the Affymetrix Fluidics Station 450 and Hybridization Oven 640 under standard conditions.
| | Sample_scan_protocol | Scanning was performed according to the standard Affymetrix protocol.
| | Sample_data_processing | The raw data was first processed with the Affymetrix Microarray Suite 5 (Affymetrix) software in order to create lists of good quality genes. The initial selection procedure utilized Affymetrix expression level flags (present, marginal, absent). Genes that were flagged either present or marginally expressed in all replicates were considered expressed and genes that were flagged absent in all replicates were considered absent. Genes with inconsistent flagging were not included in the analysis. Further, a cross gene error model was devised with the GeneSpring GX 7.3.1 (Agilent Technologies) software in order to calculate the lowest reliable expression value for each set of chips and to filter away the genes with unreliably low intensities. The raw data was then reprocessed with GeneSpring GX 7.3.1 using the GC-RMA algorithm with default parameters. The pre-processed intensities that had a magnitude lower than 0.01 were set to 0.01. The intensities of individual genes were normalized by dividing by the median intensity of a set of appropriate replicates. The resulting data was log2-transformed and geometrical means were calculated to give one expression value for a set of replicates.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Niko,Kristian,Sillanpaa
| | Sample_contact_email | niko.sillanpaa@iki.fi
| | Sample_contact_department | Molecular immunology
| | Sample_contact_institute | National Institute of Health, Finland
| | Sample_contact_address | Papinkatu 14 A 10
| | Sample_contact_city | Tampere
| | Sample_contact_zip/postal_code | 33200
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285730/suppl/GSM285730.CEL.gz
| | Sample_series_id | GSE11309
| | Sample_data_row_count | 12757
| | |
|
GSM285731 | GPL570 |
|
moDC_candida-_patient_2
|
mature moDCs from APECED patient
|
Gender: female
|
Matrix: candida neg
|
| Sample_geo_accession | GSM285731
| | Sample_status | Public on Oct 01 2008
| | Sample_submission_date | Apr 30 2008
| | Sample_last_update_date | Apr 30 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples labelled candida_pos were co-cultured with candida albicans using a cell-to-candida ratio of 1:1 (MOI of 1).
| | Sample_growth_protocol_ch1 | Peripheral blood mononuclear cells (PBMC) were isolated from 70-100 ml of EDTA blood over Ficoll–Paque gradient. PBMCs were plated at a cell density of 10×106 cells/ml onto six-well, 24-well or 96-well plates. Monocytes were allowed to adhere for 2 h at 37°C, after which non-adherent cells were removed by washing with phosphate-buffered saline (PBS). Monocytes were differentiated into moDCs in RPMI supplemented with 2 mM L-glutamine, 20 mM HEPES, 10% fetal calf serum (FCS) and containing 20 ng/ml IL-4 and 10 ng/ml GM-CSF. Fresh medium was added every two days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from DCs using the RNeasy Mini kit (Qiagen, Venlo, The Netherlands). Quantity of RNA was determined with spectrophotometer and the quality was controlled using the Agilent Bioanalyzer 2100.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total cellular RNA was treated according to the conventional Affymetrix eukaryotic RNA labeling protocols (Affymetrix, Santa Clara, CA) and then used to prepare biotinylated cRNA with the Enzo Bioarray High Yield RNA transcript labelling kit (Enzo, Life sciences, Farmingdale, NY).
| | Sample_hyb_protocol | 15 μg of biotin-labeled cRNA were fragmented according to the Affymetrix eukaryotic sample protocol and hybridized to the U133 Plus 2.0 chip (Affymetrix). Hybridization, staining and washing were performed using the Affymetrix Fluidics Station 450 and Hybridization Oven 640 under standard conditions.
| | Sample_scan_protocol | Scanning was performed according to the standard Affymetrix protocol.
| | Sample_data_processing | The raw data was first processed with the Affymetrix Microarray Suite 5 (Affymetrix) software in order to create lists of good quality genes. The initial selection procedure utilized Affymetrix expression level flags (present, marginal, absent). Genes that were flagged either present or marginally expressed in all replicates were considered expressed and genes that were flagged absent in all replicates were considered absent. Genes with inconsistent flagging were not included in the analysis. Further, a cross gene error model was devised with the GeneSpring GX 7.3.1 (Agilent Technologies) software in order to calculate the lowest reliable expression value for each set of chips and to filter away the genes with unreliably low intensities. The raw data was then reprocessed with GeneSpring GX 7.3.1 using the GC-RMA algorithm with default parameters. The pre-processed intensities that had a magnitude lower than 0.01 were set to 0.01. The intensities of individual genes were normalized by dividing by the median intensity of a set of appropriate replicates. The resulting data was log2-transformed and geometrical means were calculated to give one expression value for a set of replicates.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Niko,Kristian,Sillanpaa
| | Sample_contact_email | niko.sillanpaa@iki.fi
| | Sample_contact_department | Molecular immunology
| | Sample_contact_institute | National Institute of Health, Finland
| | Sample_contact_address | Papinkatu 14 A 10
| | Sample_contact_city | Tampere
| | Sample_contact_zip/postal_code | 33200
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285731/suppl/GSM285731.CEL.gz
| | Sample_series_id | GSE11309
| | Sample_data_row_count | 12757
| | |
|
GSM285732 | GPL570 |
|
moDC_candida-_patient_3
|
mature moDCs from APECED patient
|
Gender: female
|
Matrix: candida neg
|
| Sample_geo_accession | GSM285732
| | Sample_status | Public on Oct 01 2008
| | Sample_submission_date | Apr 30 2008
| | Sample_last_update_date | Apr 30 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples labelled candida_pos were co-cultured with candida albicans using a cell-to-candida ratio of 1:1 (MOI of 1).
| | Sample_growth_protocol_ch1 | Peripheral blood mononuclear cells (PBMC) were isolated from 70-100 ml of EDTA blood over Ficoll–Paque gradient. PBMCs were plated at a cell density of 10×106 cells/ml onto six-well, 24-well or 96-well plates. Monocytes were allowed to adhere for 2 h at 37°C, after which non-adherent cells were removed by washing with phosphate-buffered saline (PBS). Monocytes were differentiated into moDCs in RPMI supplemented with 2 mM L-glutamine, 20 mM HEPES, 10% fetal calf serum (FCS) and containing 20 ng/ml IL-4 and 10 ng/ml GM-CSF. Fresh medium was added every two days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from DCs using the RNeasy Mini kit (Qiagen, Venlo, The Netherlands). Quantity of RNA was determined with spectrophotometer and the quality was controlled using the Agilent Bioanalyzer 2100.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total cellular RNA was treated according to the conventional Affymetrix eukaryotic RNA labeling protocols (Affymetrix, Santa Clara, CA) and then used to prepare biotinylated cRNA with the Enzo Bioarray High Yield RNA transcript labelling kit (Enzo, Life sciences, Farmingdale, NY).
| | Sample_hyb_protocol | 15 μg of biotin-labeled cRNA were fragmented according to the Affymetrix eukaryotic sample protocol and hybridized to the U133 Plus 2.0 chip (Affymetrix). Hybridization, staining and washing were performed using the Affymetrix Fluidics Station 450 and Hybridization Oven 640 under standard conditions.
| | Sample_scan_protocol | Scanning was performed according to the standard Affymetrix protocol.
| | Sample_data_processing | The raw data was first processed with the Affymetrix Microarray Suite 5 (Affymetrix) software in order to create lists of good quality genes. The initial selection procedure utilized Affymetrix expression level flags (present, marginal, absent). Genes that were flagged either present or marginally expressed in all replicates were considered expressed and genes that were flagged absent in all replicates were considered absent. Genes with inconsistent flagging were not included in the analysis. Further, a cross gene error model was devised with the GeneSpring GX 7.3.1 (Agilent Technologies) software in order to calculate the lowest reliable expression value for each set of chips and to filter away the genes with unreliably low intensities. The raw data was then reprocessed with GeneSpring GX 7.3.1 using the GC-RMA algorithm with default parameters. The pre-processed intensities that had a magnitude lower than 0.01 were set to 0.01. The intensities of individual genes were normalized by dividing by the median intensity of a set of appropriate replicates. The resulting data was log2-transformed and geometrical means were calculated to give one expression value for a set of replicates.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Niko,Kristian,Sillanpaa
| | Sample_contact_email | niko.sillanpaa@iki.fi
| | Sample_contact_department | Molecular immunology
| | Sample_contact_institute | National Institute of Health, Finland
| | Sample_contact_address | Papinkatu 14 A 10
| | Sample_contact_city | Tampere
| | Sample_contact_zip/postal_code | 33200
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285732/suppl/GSM285732.CEL.gz
| | Sample_series_id | GSE11309
| | Sample_data_row_count | 12757
| | |
|
GSM285733 | GPL570 |
|
moDC_candida-_control_1
|
mature moDCs from healthy control
|
Gender: female
|
Matrix: candida neg
|
| Sample_geo_accession | GSM285733
| | Sample_status | Public on Oct 01 2008
| | Sample_submission_date | Apr 30 2008
| | Sample_last_update_date | Apr 30 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples labelled candida_pos were co-cultured with candida albicans using a cell-to-candida ratio of 1:1 (MOI of 1).
| | Sample_growth_protocol_ch1 | Peripheral blood mononuclear cells (PBMC) were isolated from 70-100 ml of EDTA blood over Ficoll–Paque gradient. PBMCs were plated at a cell density of 10×106 cells/ml onto six-well, 24-well or 96-well plates. Monocytes were allowed to adhere for 2 h at 37°C, after which non-adherent cells were removed by washing with phosphate-buffered saline (PBS). Monocytes were differentiated into moDCs in RPMI supplemented with 2 mM L-glutamine, 20 mM HEPES, 10% fetal calf serum (FCS) and containing 20 ng/ml IL-4 and 10 ng/ml GM-CSF. Fresh medium was added every two days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from DCs using the RNeasy Mini kit (Qiagen, Venlo, The Netherlands). Quantity of RNA was determined with spectrophotometer and the quality was controlled using the Agilent Bioanalyzer 2100.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total cellular RNA was treated according to the conventional Affymetrix eukaryotic RNA labeling protocols (Affymetrix, Santa Clara, CA) and then used to prepare biotinylated cRNA with the Enzo Bioarray High Yield RNA transcript labelling kit (Enzo, Life sciences, Farmingdale, NY).
| | Sample_hyb_protocol | 15 μg of biotin-labeled cRNA were fragmented according to the Affymetrix eukaryotic sample protocol and hybridized to the U133 Plus 2.0 chip (Affymetrix). Hybridization, staining and washing were performed using the Affymetrix Fluidics Station 450 and Hybridization Oven 640 under standard conditions.
| | Sample_scan_protocol | Scanning was performed according to the standard Affymetrix protocol.
| | Sample_data_processing | The raw data was first processed with the Affymetrix Microarray Suite 5 (Affymetrix) software in order to create lists of good quality genes. The initial selection procedure utilized Affymetrix expression level flags (present, marginal, absent). Genes that were flagged either present or marginally expressed in all replicates were considered expressed and genes that were flagged absent in all replicates were considered absent. Genes with inconsistent flagging were not included in the analysis. Further, a cross gene error model was devised with the GeneSpring GX 7.3.1 (Agilent Technologies) software in order to calculate the lowest reliable expression value for each set of chips and to filter away the genes with unreliably low intensities. The raw data was then reprocessed with GeneSpring GX 7.3.1 using the GC-RMA algorithm with default parameters. The pre-processed intensities that had a magnitude lower than 0.01 were set to 0.01. The intensities of individual genes were normalized by dividing by the median intensity of a set of appropriate replicates. The resulting data was log2-transformed and geometrical means were calculated to give one expression value for a set of replicates.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Niko,Kristian,Sillanpaa
| | Sample_contact_email | niko.sillanpaa@iki.fi
| | Sample_contact_department | Molecular immunology
| | Sample_contact_institute | National Institute of Health, Finland
| | Sample_contact_address | Papinkatu 14 A 10
| | Sample_contact_city | Tampere
| | Sample_contact_zip/postal_code | 33200
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285733/suppl/GSM285733.CEL.gz
| | Sample_series_id | GSE11309
| | Sample_data_row_count | 12757
| | |
|
GSM285734 | GPL570 |
|
moDC_candida-_control_2
|
mature moDCs from healthy control
|
Gender: female
|
Matrix: candida neg
|
| Sample_geo_accession | GSM285734
| | Sample_status | Public on Oct 01 2008
| | Sample_submission_date | Apr 30 2008
| | Sample_last_update_date | Apr 30 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples labelled candida_pos were co-cultured with candida albicans using a cell-to-candida ratio of 1:1 (MOI of 1).
| | Sample_growth_protocol_ch1 | Peripheral blood mononuclear cells (PBMC) were isolated from 70-100 ml of EDTA blood over Ficoll–Paque gradient. PBMCs were plated at a cell density of 10×106 cells/ml onto six-well, 24-well or 96-well plates. Monocytes were allowed to adhere for 2 h at 37°C, after which non-adherent cells were removed by washing with phosphate-buffered saline (PBS). Monocytes were differentiated into moDCs in RPMI supplemented with 2 mM L-glutamine, 20 mM HEPES, 10% fetal calf serum (FCS) and containing 20 ng/ml IL-4 and 10 ng/ml GM-CSF. Fresh medium was added every two days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from DCs using the RNeasy Mini kit (Qiagen, Venlo, The Netherlands). Quantity of RNA was determined with spectrophotometer and the quality was controlled using the Agilent Bioanalyzer 2100.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total cellular RNA was treated according to the conventional Affymetrix eukaryotic RNA labeling protocols (Affymetrix, Santa Clara, CA) and then used to prepare biotinylated cRNA with the Enzo Bioarray High Yield RNA transcript labelling kit (Enzo, Life sciences, Farmingdale, NY).
| | Sample_hyb_protocol | 15 μg of biotin-labeled cRNA were fragmented according to the Affymetrix eukaryotic sample protocol and hybridized to the U133 Plus 2.0 chip (Affymetrix). Hybridization, staining and washing were performed using the Affymetrix Fluidics Station 450 and Hybridization Oven 640 under standard conditions.
| | Sample_scan_protocol | Scanning was performed according to the standard Affymetrix protocol.
| | Sample_data_processing | The raw data was first processed with the Affymetrix Microarray Suite 5 (Affymetrix) software in order to create lists of good quality genes. The initial selection procedure utilized Affymetrix expression level flags (present, marginal, absent). Genes that were flagged either present or marginally expressed in all replicates were considered expressed and genes that were flagged absent in all replicates were considered absent. Genes with inconsistent flagging were not included in the analysis. Further, a cross gene error model was devised with the GeneSpring GX 7.3.1 (Agilent Technologies) software in order to calculate the lowest reliable expression value for each set of chips and to filter away the genes with unreliably low intensities. The raw data was then reprocessed with GeneSpring GX 7.3.1 using the GC-RMA algorithm with default parameters. The pre-processed intensities that had a magnitude lower than 0.01 were set to 0.01. The intensities of individual genes were normalized by dividing by the median intensity of a set of appropriate replicates. The resulting data was log2-transformed and geometrical means were calculated to give one expression value for a set of replicates.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Niko,Kristian,Sillanpaa
| | Sample_contact_email | niko.sillanpaa@iki.fi
| | Sample_contact_department | Molecular immunology
| | Sample_contact_institute | National Institute of Health, Finland
| | Sample_contact_address | Papinkatu 14 A 10
| | Sample_contact_city | Tampere
| | Sample_contact_zip/postal_code | 33200
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285734/suppl/GSM285734.CEL.gz
| | Sample_series_id | GSE11309
| | Sample_data_row_count | 12757
| | |
|
GSM285735 | GPL570 |
|
moDC_candida-_control_3
|
mature moDCs from healthy control
|
Gender: female
|
Matrix: candida neg
|
| Sample_geo_accession | GSM285735
| | Sample_status | Public on Oct 01 2008
| | Sample_submission_date | Apr 30 2008
| | Sample_last_update_date | Apr 30 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples labelled candida_pos were co-cultured with candida albicans using a cell-to-candida ratio of 1:1 (MOI of 1).
| | Sample_growth_protocol_ch1 | Peripheral blood mononuclear cells (PBMC) were isolated from 70-100 ml of EDTA blood over Ficoll–Paque gradient. PBMCs were plated at a cell density of 10×106 cells/ml onto six-well, 24-well or 96-well plates. Monocytes were allowed to adhere for 2 h at 37°C, after which non-adherent cells were removed by washing with phosphate-buffered saline (PBS). Monocytes were differentiated into moDCs in RPMI supplemented with 2 mM L-glutamine, 20 mM HEPES, 10% fetal calf serum (FCS) and containing 20 ng/ml IL-4 and 10 ng/ml GM-CSF. Fresh medium was added every two days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from DCs using the RNeasy Mini kit (Qiagen, Venlo, The Netherlands). Quantity of RNA was determined with spectrophotometer and the quality was controlled using the Agilent Bioanalyzer 2100.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total cellular RNA was treated according to the conventional Affymetrix eukaryotic RNA labeling protocols (Affymetrix, Santa Clara, CA) and then used to prepare biotinylated cRNA with the Enzo Bioarray High Yield RNA transcript labelling kit (Enzo, Life sciences, Farmingdale, NY).
| | Sample_hyb_protocol | 15 μg of biotin-labeled cRNA were fragmented according to the Affymetrix eukaryotic sample protocol and hybridized to the U133 Plus 2.0 chip (Affymetrix). Hybridization, staining and washing were performed using the Affymetrix Fluidics Station 450 and Hybridization Oven 640 under standard conditions.
| | Sample_scan_protocol | Scanning was performed according to the standard Affymetrix protocol.
| | Sample_data_processing | The raw data was first processed with the Affymetrix Microarray Suite 5 (Affymetrix) software in order to create lists of good quality genes. The initial selection procedure utilized Affymetrix expression level flags (present, marginal, absent). Genes that were flagged either present or marginally expressed in all replicates were considered expressed and genes that were flagged absent in all replicates were considered absent. Genes with inconsistent flagging were not included in the analysis. Further, a cross gene error model was devised with the GeneSpring GX 7.3.1 (Agilent Technologies) software in order to calculate the lowest reliable expression value for each set of chips and to filter away the genes with unreliably low intensities. The raw data was then reprocessed with GeneSpring GX 7.3.1 using the GC-RMA algorithm with default parameters. The pre-processed intensities that had a magnitude lower than 0.01 were set to 0.01. The intensities of individual genes were normalized by dividing by the median intensity of a set of appropriate replicates. The resulting data was log2-transformed and geometrical means were calculated to give one expression value for a set of replicates.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Niko,Kristian,Sillanpaa
| | Sample_contact_email | niko.sillanpaa@iki.fi
| | Sample_contact_department | Molecular immunology
| | Sample_contact_institute | National Institute of Health, Finland
| | Sample_contact_address | Papinkatu 14 A 10
| | Sample_contact_city | Tampere
| | Sample_contact_zip/postal_code | 33200
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285735/suppl/GSM285735.CEL.gz
| | Sample_series_id | GSE11309
| | Sample_data_row_count | 12757
| | |
|
GSM285736 | GPL570 |
|
moDC_candida+_patient_1
|
mature, candida albicans stimulated moDCs from APECED patient
|
Gender: female
|
Matrix: candida pos
|
| Sample_geo_accession | GSM285736
| | Sample_status | Public on Oct 01 2008
| | Sample_submission_date | Apr 30 2008
| | Sample_last_update_date | Apr 30 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples labelled candida_pos were co-cultured with candida albicans using a cell-to-candida ratio of 1:1 (MOI of 1).
| | Sample_growth_protocol_ch1 | Peripheral blood mononuclear cells (PBMC) were isolated from 70-100 ml of EDTA blood over Ficoll–Paque gradient. PBMCs were plated at a cell density of 10×106 cells/ml onto six-well, 24-well or 96-well plates. Monocytes were allowed to adhere for 2 h at 37°C, after which non-adherent cells were removed by washing with phosphate-buffered saline (PBS). Monocytes were differentiated into moDCs in RPMI supplemented with 2 mM L-glutamine, 20 mM HEPES, 10% fetal calf serum (FCS) and containing 20 ng/ml IL-4 and 10 ng/ml GM-CSF. Fresh medium was added every two days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from DCs using the RNeasy Mini kit (Qiagen, Venlo, The Netherlands). Quantity of RNA was determined with spectrophotometer and the quality was controlled using the Agilent Bioanalyzer 2100.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total cellular RNA was treated according to the conventional Affymetrix eukaryotic RNA labeling protocols (Affymetrix, Santa Clara, CA) and then used to prepare biotinylated cRNA with the Enzo Bioarray High Yield RNA transcript labelling kit (Enzo, Life sciences, Farmingdale, NY).
| | Sample_hyb_protocol | 15 μg of biotin-labeled cRNA were fragmented according to the Affymetrix eukaryotic sample protocol and hybridized to the U133 Plus 2.0 chip (Affymetrix). Hybridization, staining and washing were performed using the Affymetrix Fluidics Station 450 and Hybridization Oven 640 under standard conditions.
| | Sample_scan_protocol | Scanning was performed according to the standard Affymetrix protocol.
| | Sample_data_processing | The raw data was first processed with the Affymetrix Microarray Suite 5 (Affymetrix) software in order to create lists of good quality genes. The initial selection procedure utilized Affymetrix expression level flags (present, marginal, absent). Genes that were flagged either present or marginally expressed in all replicates were considered expressed and genes that were flagged absent in all replicates were considered absent. Genes with inconsistent flagging were not included in the analysis. Further, a cross gene error model was devised with the GeneSpring GX 7.3.1 (Agilent Technologies) software in order to calculate the lowest reliable expression value for each set of chips and to filter away the genes with unreliably low intensities. The raw data was then reprocessed with GeneSpring GX 7.3.1 using the GC-RMA algorithm with default parameters. The pre-processed intensities that had a magnitude lower than 0.01 were set to 0.01. The intensities of individual genes were normalized by dividing by the median intensity of a set of appropriate replicates. The resulting data was log2-transformed and geometrical means were calculated to give one expression value for a set of replicates.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Niko,Kristian,Sillanpaa
| | Sample_contact_email | niko.sillanpaa@iki.fi
| | Sample_contact_department | Molecular immunology
| | Sample_contact_institute | National Institute of Health, Finland
| | Sample_contact_address | Papinkatu 14 A 10
| | Sample_contact_city | Tampere
| | Sample_contact_zip/postal_code | 33200
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285736/suppl/GSM285736.CEL.gz
| | Sample_series_id | GSE11309
| | Sample_data_row_count | 12681
| | |
|
GSM285737 | GPL570 |
|
moDC_candida+_patient_2
|
mature, candida albicans stimulated moDCs from APECED patient
|
Gender: female
|
Matrix: candida pos
|
| Sample_geo_accession | GSM285737
| | Sample_status | Public on Oct 01 2008
| | Sample_submission_date | Apr 30 2008
| | Sample_last_update_date | Apr 30 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples labelled candida_pos were co-cultured with candida albicans using a cell-to-candida ratio of 1:1 (MOI of 1).
| | Sample_growth_protocol_ch1 | Peripheral blood mononuclear cells (PBMC) were isolated from 70-100 ml of EDTA blood over Ficoll–Paque gradient. PBMCs were plated at a cell density of 10×106 cells/ml onto six-well, 24-well or 96-well plates. Monocytes were allowed to adhere for 2 h at 37°C, after which non-adherent cells were removed by washing with phosphate-buffered saline (PBS). Monocytes were differentiated into moDCs in RPMI supplemented with 2 mM L-glutamine, 20 mM HEPES, 10% fetal calf serum (FCS) and containing 20 ng/ml IL-4 and 10 ng/ml GM-CSF. Fresh medium was added every two days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from DCs using the RNeasy Mini kit (Qiagen, Venlo, The Netherlands). Quantity of RNA was determined with spectrophotometer and the quality was controlled using the Agilent Bioanalyzer 2100.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total cellular RNA was treated according to the conventional Affymetrix eukaryotic RNA labeling protocols (Affymetrix, Santa Clara, CA) and then used to prepare biotinylated cRNA with the Enzo Bioarray High Yield RNA transcript labelling kit (Enzo, Life sciences, Farmingdale, NY).
| | Sample_hyb_protocol | 15 μg of biotin-labeled cRNA were fragmented according to the Affymetrix eukaryotic sample protocol and hybridized to the U133 Plus 2.0 chip (Affymetrix). Hybridization, staining and washing were performed using the Affymetrix Fluidics Station 450 and Hybridization Oven 640 under standard conditions.
| | Sample_scan_protocol | Scanning was performed according to the standard Affymetrix protocol.
| | Sample_data_processing | The raw data was first processed with the Affymetrix Microarray Suite 5 (Affymetrix) software in order to create lists of good quality genes. The initial selection procedure utilized Affymetrix expression level flags (present, marginal, absent). Genes that were flagged either present or marginally expressed in all replicates were considered expressed and genes that were flagged absent in all replicates were considered absent. Genes with inconsistent flagging were not included in the analysis. Further, a cross gene error model was devised with the GeneSpring GX 7.3.1 (Agilent Technologies) software in order to calculate the lowest reliable expression value for each set of chips and to filter away the genes with unreliably low intensities. The raw data was then reprocessed with GeneSpring GX 7.3.1 using the GC-RMA algorithm with default parameters. The pre-processed intensities that had a magnitude lower than 0.01 were set to 0.01. The intensities of individual genes were normalized by dividing by the median intensity of a set of appropriate replicates. The resulting data was log2-transformed and geometrical means were calculated to give one expression value for a set of replicates.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Niko,Kristian,Sillanpaa
| | Sample_contact_email | niko.sillanpaa@iki.fi
| | Sample_contact_department | Molecular immunology
| | Sample_contact_institute | National Institute of Health, Finland
| | Sample_contact_address | Papinkatu 14 A 10
| | Sample_contact_city | Tampere
| | Sample_contact_zip/postal_code | 33200
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285737/suppl/GSM285737.CEL.gz
| | Sample_series_id | GSE11309
| | Sample_data_row_count | 12681
| | |
|
GSM285738 | GPL570 |
|
moDC_candida+_patient_3
|
mature, candida albicans stimulated moDCs from APECED patient
|
Gender: female
|
Matrix: candida pos
|
| Sample_geo_accession | GSM285738
| | Sample_status | Public on Oct 01 2008
| | Sample_submission_date | Apr 30 2008
| | Sample_last_update_date | Apr 30 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples labelled candida_pos were co-cultured with candida albicans using a cell-to-candida ratio of 1:1 (MOI of 1).
| | Sample_growth_protocol_ch1 | Peripheral blood mononuclear cells (PBMC) were isolated from 70-100 ml of EDTA blood over Ficoll–Paque gradient. PBMCs were plated at a cell density of 10×106 cells/ml onto six-well, 24-well or 96-well plates. Monocytes were allowed to adhere for 2 h at 37°C, after which non-adherent cells were removed by washing with phosphate-buffered saline (PBS). Monocytes were differentiated into moDCs in RPMI supplemented with 2 mM L-glutamine, 20 mM HEPES, 10% fetal calf serum (FCS) and containing 20 ng/ml IL-4 and 10 ng/ml GM-CSF. Fresh medium was added every two days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from DCs using the RNeasy Mini kit (Qiagen, Venlo, The Netherlands). Quantity of RNA was determined with spectrophotometer and the quality was controlled using the Agilent Bioanalyzer 2100.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total cellular RNA was treated according to the conventional Affymetrix eukaryotic RNA labeling protocols (Affymetrix, Santa Clara, CA) and then used to prepare biotinylated cRNA with the Enzo Bioarray High Yield RNA transcript labelling kit (Enzo, Life sciences, Farmingdale, NY).
| | Sample_hyb_protocol | 15 μg of biotin-labeled cRNA were fragmented according to the Affymetrix eukaryotic sample protocol and hybridized to the U133 Plus 2.0 chip (Affymetrix). Hybridization, staining and washing were performed using the Affymetrix Fluidics Station 450 and Hybridization Oven 640 under standard conditions.
| | Sample_scan_protocol | Scanning was performed according to the standard Affymetrix protocol.
| | Sample_data_processing | The raw data was first processed with the Affymetrix Microarray Suite 5 (Affymetrix) software in order to create lists of good quality genes. The initial selection procedure utilized Affymetrix expression level flags (present, marginal, absent). Genes that were flagged either present or marginally expressed in all replicates were considered expressed and genes that were flagged absent in all replicates were considered absent. Genes with inconsistent flagging were not included in the analysis. Further, a cross gene error model was devised with the GeneSpring GX 7.3.1 (Agilent Technologies) software in order to calculate the lowest reliable expression value for each set of chips and to filter away the genes with unreliably low intensities. The raw data was then reprocessed with GeneSpring GX 7.3.1 using the GC-RMA algorithm with default parameters. The pre-processed intensities that had a magnitude lower than 0.01 were set to 0.01. The intensities of individual genes were normalized by dividing by the median intensity of a set of appropriate replicates. The resulting data was log2-transformed and geometrical means were calculated to give one expression value for a set of replicates.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Niko,Kristian,Sillanpaa
| | Sample_contact_email | niko.sillanpaa@iki.fi
| | Sample_contact_department | Molecular immunology
| | Sample_contact_institute | National Institute of Health, Finland
| | Sample_contact_address | Papinkatu 14 A 10
| | Sample_contact_city | Tampere
| | Sample_contact_zip/postal_code | 33200
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285738/suppl/GSM285738.CEL.gz
| | Sample_series_id | GSE11309
| | Sample_data_row_count | 12681
| | |
|
GSM285739 | GPL570 |
|
moDC_candida+_control_1
|
mature, candida albicans stimulated moDCs from healthy control
|
Gender: female
|
Matrix: candida pos
|
| Sample_geo_accession | GSM285739
| | Sample_status | Public on Oct 01 2008
| | Sample_submission_date | Apr 30 2008
| | Sample_last_update_date | Apr 30 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples labelled candida_pos were co-cultured with candida albicans using a cell-to-candida ratio of 1:1 (MOI of 1).
| | Sample_growth_protocol_ch1 | Peripheral blood mononuclear cells (PBMC) were isolated from 70-100 ml of EDTA blood over Ficoll–Paque gradient. PBMCs were plated at a cell density of 10×106 cells/ml onto six-well, 24-well or 96-well plates. Monocytes were allowed to adhere for 2 h at 37°C, after which non-adherent cells were removed by washing with phosphate-buffered saline (PBS). Monocytes were differentiated into moDCs in RPMI supplemented with 2 mM L-glutamine, 20 mM HEPES, 10% fetal calf serum (FCS) and containing 20 ng/ml IL-4 and 10 ng/ml GM-CSF. Fresh medium was added every two days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from DCs using the RNeasy Mini kit (Qiagen, Venlo, The Netherlands). Quantity of RNA was determined with spectrophotometer and the quality was controlled using the Agilent Bioanalyzer 2100.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total cellular RNA was treated according to the conventional Affymetrix eukaryotic RNA labeling protocols (Affymetrix, Santa Clara, CA) and then used to prepare biotinylated cRNA with the Enzo Bioarray High Yield RNA transcript labelling kit (Enzo, Life sciences, Farmingdale, NY).
| | Sample_hyb_protocol | 15 μg of biotin-labeled cRNA were fragmented according to the Affymetrix eukaryotic sample protocol and hybridized to the U133 Plus 2.0 chip (Affymetrix). Hybridization, staining and washing were performed using the Affymetrix Fluidics Station 450 and Hybridization Oven 640 under standard conditions.
| | Sample_scan_protocol | Scanning was performed according to the standard Affymetrix protocol.
| | Sample_data_processing | The raw data was first processed with the Affymetrix Microarray Suite 5 (Affymetrix) software in order to create lists of good quality genes. The initial selection procedure utilized Affymetrix expression level flags (present, marginal, absent). Genes that were flagged either present or marginally expressed in all replicates were considered expressed and genes that were flagged absent in all replicates were considered absent. Genes with inconsistent flagging were not included in the analysis. Further, a cross gene error model was devised with the GeneSpring GX 7.3.1 (Agilent Technologies) software in order to calculate the lowest reliable expression value for each set of chips and to filter away the genes with unreliably low intensities. The raw data was then reprocessed with GeneSpring GX 7.3.1 using the GC-RMA algorithm with default parameters. The pre-processed intensities that had a magnitude lower than 0.01 were set to 0.01. The intensities of individual genes were normalized by dividing by the median intensity of a set of appropriate replicates. The resulting data was log2-transformed and geometrical means were calculated to give one expression value for a set of replicates.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Niko,Kristian,Sillanpaa
| | Sample_contact_email | niko.sillanpaa@iki.fi
| | Sample_contact_department | Molecular immunology
| | Sample_contact_institute | National Institute of Health, Finland
| | Sample_contact_address | Papinkatu 14 A 10
| | Sample_contact_city | Tampere
| | Sample_contact_zip/postal_code | 33200
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285739/suppl/GSM285739.CEL.gz
| | Sample_series_id | GSE11309
| | Sample_data_row_count | 12681
| | |
|
GSM285740 | GPL570 |
|
moDC_candida+_control_2
|
mature, candida albicans stimulated moDCs from healthy control
|
Gender: female
|
Matrix: candida pos
|
| Sample_geo_accession | GSM285740
| | Sample_status | Public on Oct 01 2008
| | Sample_submission_date | Apr 30 2008
| | Sample_last_update_date | Apr 30 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples labelled candida_pos were co-cultured with candida albicans using a cell-to-candida ratio of 1:1 (MOI of 1).
| | Sample_growth_protocol_ch1 | Peripheral blood mononuclear cells (PBMC) were isolated from 70-100 ml of EDTA blood over Ficoll–Paque gradient. PBMCs were plated at a cell density of 10×106 cells/ml onto six-well, 24-well or 96-well plates. Monocytes were allowed to adhere for 2 h at 37°C, after which non-adherent cells were removed by washing with phosphate-buffered saline (PBS). Monocytes were differentiated into moDCs in RPMI supplemented with 2 mM L-glutamine, 20 mM HEPES, 10% fetal calf serum (FCS) and containing 20 ng/ml IL-4 and 10 ng/ml GM-CSF. Fresh medium was added every two days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from DCs using the RNeasy Mini kit (Qiagen, Venlo, The Netherlands). Quantity of RNA was determined with spectrophotometer and the quality was controlled using the Agilent Bioanalyzer 2100.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total cellular RNA was treated according to the conventional Affymetrix eukaryotic RNA labeling protocols (Affymetrix, Santa Clara, CA) and then used to prepare biotinylated cRNA with the Enzo Bioarray High Yield RNA transcript labelling kit (Enzo, Life sciences, Farmingdale, NY).
| | Sample_hyb_protocol | 15 μg of biotin-labeled cRNA were fragmented according to the Affymetrix eukaryotic sample protocol and hybridized to the U133 Plus 2.0 chip (Affymetrix). Hybridization, staining and washing were performed using the Affymetrix Fluidics Station 450 and Hybridization Oven 640 under standard conditions.
| | Sample_scan_protocol | Scanning was performed according to the standard Affymetrix protocol.
| | Sample_data_processing | The raw data was first processed with the Affymetrix Microarray Suite 5 (Affymetrix) software in order to create lists of good quality genes. The initial selection procedure utilized Affymetrix expression level flags (present, marginal, absent). Genes that were flagged either present or marginally expressed in all replicates were considered expressed and genes that were flagged absent in all replicates were considered absent. Genes with inconsistent flagging were not included in the analysis. Further, a cross gene error model was devised with the GeneSpring GX 7.3.1 (Agilent Technologies) software in order to calculate the lowest reliable expression value for each set of chips and to filter away the genes with unreliably low intensities. The raw data was then reprocessed with GeneSpring GX 7.3.1 using the GC-RMA algorithm with default parameters. The pre-processed intensities that had a magnitude lower than 0.01 were set to 0.01. The intensities of individual genes were normalized by dividing by the median intensity of a set of appropriate replicates. The resulting data was log2-transformed and geometrical means were calculated to give one expression value for a set of replicates.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Niko,Kristian,Sillanpaa
| | Sample_contact_email | niko.sillanpaa@iki.fi
| | Sample_contact_department | Molecular immunology
| | Sample_contact_institute | National Institute of Health, Finland
| | Sample_contact_address | Papinkatu 14 A 10
| | Sample_contact_city | Tampere
| | Sample_contact_zip/postal_code | 33200
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285740/suppl/GSM285740.CEL.gz
| | Sample_series_id | GSE11309
| | Sample_data_row_count | 12681
| | |
|
GSM285741 | GPL570 |
|
moDC_candida+_control_3
|
mature, candida albicans stimulated moDCs from healthy control
|
Gender: female
|
Matrix: candida pos
|
| Sample_geo_accession | GSM285741
| | Sample_status | Public on Oct 01 2008
| | Sample_submission_date | Apr 30 2008
| | Sample_last_update_date | Apr 30 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The samples labelled candida_pos were co-cultured with candida albicans using a cell-to-candida ratio of 1:1 (MOI of 1).
| | Sample_growth_protocol_ch1 | Peripheral blood mononuclear cells (PBMC) were isolated from 70-100 ml of EDTA blood over Ficoll–Paque gradient. PBMCs were plated at a cell density of 10×106 cells/ml onto six-well, 24-well or 96-well plates. Monocytes were allowed to adhere for 2 h at 37°C, after which non-adherent cells were removed by washing with phosphate-buffered saline (PBS). Monocytes were differentiated into moDCs in RPMI supplemented with 2 mM L-glutamine, 20 mM HEPES, 10% fetal calf serum (FCS) and containing 20 ng/ml IL-4 and 10 ng/ml GM-CSF. Fresh medium was added every two days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from DCs using the RNeasy Mini kit (Qiagen, Venlo, The Netherlands). Quantity of RNA was determined with spectrophotometer and the quality was controlled using the Agilent Bioanalyzer 2100.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of total cellular RNA was treated according to the conventional Affymetrix eukaryotic RNA labeling protocols (Affymetrix, Santa Clara, CA) and then used to prepare biotinylated cRNA with the Enzo Bioarray High Yield RNA transcript labelling kit (Enzo, Life sciences, Farmingdale, NY).
| | Sample_hyb_protocol | 15 μg of biotin-labeled cRNA were fragmented according to the Affymetrix eukaryotic sample protocol and hybridized to the U133 Plus 2.0 chip (Affymetrix). Hybridization, staining and washing were performed using the Affymetrix Fluidics Station 450 and Hybridization Oven 640 under standard conditions.
| | Sample_scan_protocol | Scanning was performed according to the standard Affymetrix protocol.
| | Sample_data_processing | The raw data was first processed with the Affymetrix Microarray Suite 5 (Affymetrix) software in order to create lists of good quality genes. The initial selection procedure utilized Affymetrix expression level flags (present, marginal, absent). Genes that were flagged either present or marginally expressed in all replicates were considered expressed and genes that were flagged absent in all replicates were considered absent. Genes with inconsistent flagging were not included in the analysis. Further, a cross gene error model was devised with the GeneSpring GX 7.3.1 (Agilent Technologies) software in order to calculate the lowest reliable expression value for each set of chips and to filter away the genes with unreliably low intensities. The raw data was then reprocessed with GeneSpring GX 7.3.1 using the GC-RMA algorithm with default parameters. The pre-processed intensities that had a magnitude lower than 0.01 were set to 0.01. The intensities of individual genes were normalized by dividing by the median intensity of a set of appropriate replicates. The resulting data was log2-transformed and geometrical means were calculated to give one expression value for a set of replicates.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Niko,Kristian,Sillanpaa
| | Sample_contact_email | niko.sillanpaa@iki.fi
| | Sample_contact_department | Molecular immunology
| | Sample_contact_institute | National Institute of Health, Finland
| | Sample_contact_address | Papinkatu 14 A 10
| | Sample_contact_city | Tampere
| | Sample_contact_zip/postal_code | 33200
| | Sample_contact_country | Finland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285741/suppl/GSM285741.CEL.gz
| | Sample_series_id | GSE11309
| | Sample_data_row_count | 12681
| | |
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