Search results for the GEO ID: GSE11314  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM285881 | GPL1261 |
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E12.5 gonad-mesonephros normal
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embryonic gonad-mesonephros
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Wild-type ovary-mesonephros
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Gonad-mesonephric complexes from E12.5 XX wild type embryos
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| Sample_geo_accession | GSM285881
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | May 01 2008
| | Sample_last_update_date | May 05 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Nikolay Manuylov
| | Sample_treatment_protocol_ch1 | Gonad-mesonephric complexes were dissected from E12.5 XX wild type embryos
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA isolated from the 6 pairs of wild type complexes using RNeasy Mini kit (Qiagen). Total RNA quantity was determined using the NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies Inc.)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesized cRNA were labeled during transcription (Megascript system; Ambion) with biotin-11–cytidine triphosphate and biotin-16–uridine triphosphate (Enzo Diagnostics).
| | Sample_hyb_protocol | Biotinylated cRNA products were hybridized to Affymetrix GeneChip mouse arrays for 16 h at 40°C in a Gene Chip Hybridization oven 640 using the manufacturer's hybridization buffer (Affymetrix).
| | Sample_scan_protocol | After hybridization, the arrays were washed and stained using the Fluidics Station 450 (Affymetrix). Chips were scanned with the 7G Affymetrix Gene Scanner (Affymetrix) according to manufacturer's recommendation.
| | Sample_data_processing | MAS5.0 algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Sergei,G,Tevosian
| | Sample_contact_email | tevosian@dartmouth.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Dartmouth Medical School
| | Sample_contact_address | 7400 Hinman Box
| | Sample_contact_city | Hanover
| | Sample_contact_state | NH
| | Sample_contact_zip/postal_code | 03755
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285881/suppl/GSM285881.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285881/suppl/GSM285881.CHP.gz
| | Sample_series_id | GSE11314
| | Sample_data_row_count | 45101
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GSM285882 | GPL1261 |
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E12.5 XX Gata4ki/ki gonad-mesonephros
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E12.5 XX Gata4ki/ki gonad-mesonephros
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Strain: C57Bl/6
Age: E12.5
Tissue: Gonad-mesonephros
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Gonad-mesonephric complexes from E12.5 XX Gata4ki/ki embryos
|
| Sample_geo_accession | GSM285882
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | May 01 2008
| | Sample_last_update_date | May 05 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Nikolay Manuylov
| | Sample_treatment_protocol_ch1 | Gonad-mesonephric complexes were dissected from E12.5 XX Gata4ki/ki embryos
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA isolated from the 6 pairs of Gata4ki/ki complexes using RNeasy Mini kit (Qiagen). Total RNA quantity was determined using the NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies Inc.)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Synthesized cRNA were labeled during transcription (Megascript system; Ambion) with biotin-11–cytidine triphosphate and biotin-16–uridine triphosphate (Enzo Diagnostics).
| | Sample_hyb_protocol | Biotinylated cRNA products were hybridized to Affymetrix GeneChip mouse arrays for 16 h at 40°C in a Gene Chip Hybridization oven 640 using the manufacturer's hybridization buffer (Affymetrix).
| | Sample_scan_protocol | After hybridization, the arrays were washed and stained using the Fluidics Station 450 (Affymetrix). Chips were scanned with the 7G Affymetrix Gene Scanner (Affymetrix) according to manufacturer's recommendation.
| | Sample_data_processing | MAS5.0 algorithm
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Sergei,G,Tevosian
| | Sample_contact_email | tevosian@dartmouth.edu
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Dartmouth Medical School
| | Sample_contact_address | 7400 Hinman Box
| | Sample_contact_city | Hanover
| | Sample_contact_state | NH
| | Sample_contact_zip/postal_code | 03755
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285882/suppl/GSM285882.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM285nnn/GSM285882/suppl/GSM285882.CHP.gz
| | Sample_series_id | GSE11314
| | Sample_data_row_count | 45101
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