Result

Search results for the GEO ID: GSE11330

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GPL ID

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Title

Source name

Description

Characteristics

GSM286134

GPL570

MCF-7 transfected with pcDNA3.1

Hman Breast Cancer MCF-7 Cells, transfected with pcDNA3.1

Human Breast Cancer Cells

Gene expression data from MCF-7 transfected with pcDNA3.1

Sample_geo_accession	GSM286134
Sample_status	Public on May 02 2009
Sample_submission_date	May 02 2008
Sample_last_update_date	May 05 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were transfected with the control and SOX2 expression plasmids by using Lipofectamine 2000 reagent (Invitrogen) in accordance with the manufacturer's protocol. The cells were harvested at 24 hours after transfection.
Sample_growth_protocol_ch1	The MCF-7 cells were grown in Dulbecco's modified Eagle's medium (Invitrogen) supplemented with 10% fetal bovine serum (Gibco BRL). The cells were maintained in a humidified 37°C incubator with 5% CO2, fed every 3 days with complete medium, and subcultured when confluence was reached.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	2 μg of total RNA was used to synthesize double-stranded cDNA, then to produce biotin-tagged cRNA using MessageAmp™ II aRNA Amplification Kit.
Sample_hyb_protocol	Hybridization was performed at 45°C with rotation for 16 h (Affymetrix GeneChip Hybridization Oven 640). The GeneChip arrays were washed and then stained (streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000
Sample_data_processing	The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). The scanned images were firstly assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of GCOS 1.4. A global scaling factor of 500 was used to normalize the different arrays.
Sample_platform_id	GPL570
Sample_contact_name	Yupeng,,Chen
Sample_contact_institute	Peking University Health Science Center
Sample_contact_address	Haidian District 38
Sample_contact_city	Beijing
Sample_contact_zip/postal_code	100083
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286134/suppl/GSM286134.CEL.gz
Sample_series_id	GSE11330
Sample_data_row_count	54675

GSM286135

GPL570

MCF-7 transfected with human SOX2 expression construct,biological rep1

Hman Breast Cancer MCF-7 Cells, transfected with Human SOX2 Expression Construct

Human Breast Cancer Cells

Gene expression data from MCF-7 transfected with SOX2

Sample_geo_accession	GSM286135
Sample_status	Public on May 02 2009
Sample_submission_date	May 02 2008
Sample_last_update_date	May 05 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were transfected with the control and SOX2 expression plasmids by using Lipofectamine 2000 reagent (Invitrogen) in accordance with the manufacturer's protocol. The cells were harvested at 24 hours after transfection.
Sample_growth_protocol_ch1	The MCF-7 cells were grown in Dulbecco's modified Eagle's medium (Invitrogen) supplemented with 10% fetal bovine serum (Gibco BRL). The cells were maintained in a humidified 37°C incubator with 5% CO2, fed every 3 days with complete medium, and subcultured when confluence was reached.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	2 μg of total RNA was used to synthesize double-stranded cDNA, then to produce biotin-tagged cRNA using MessageAmp™ II aRNA Amplification Kit.
Sample_hyb_protocol	Hybridization was performed at 45°C with rotation for 16 h (Affymetrix GeneChip Hybridization Oven 640). The GeneChip arrays were washed and then stained (streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000
Sample_data_processing	The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). The scanned images were firstly assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of GCOS 1.4. A global scaling factor of 500 was used to normalize the different arrays.
Sample_platform_id	GPL570
Sample_contact_name	Yupeng,,Chen
Sample_contact_institute	Peking University Health Science Center
Sample_contact_address	Haidian District 38
Sample_contact_city	Beijing
Sample_contact_zip/postal_code	100083
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286135/suppl/GSM286135.CEL.gz
Sample_series_id	GSE11330
Sample_data_row_count	54675

GSM286136

GPL570

MCF-7 transfected with human SOX2 expression construct,biological rep2

Hman Breast Cancer MCF-7 Cells, transfected with Human SOX2 Expression Construct

Human Breast Cancer Cells

Gene expression data from MCF-7 transfected with SOX2

Sample_geo_accession	GSM286136
Sample_status	Public on May 02 2009
Sample_submission_date	May 02 2008
Sample_last_update_date	May 05 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were transfected with the control and SOX2 expression plasmids by using Lipofectamine 2000 reagent (Invitrogen) in accordance with the manufacturer's protocol. The cells were harvested at 24 hours after transfection.
Sample_growth_protocol_ch1	The MCF-7 cells were grown in Dulbecco's modified Eagle's medium (Invitrogen) supplemented with 10% fetal bovine serum (Gibco BRL). The cells were maintained in a humidified 37°C incubator with 5% CO2, fed every 3 days with complete medium, and subcultured when confluence was reached.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	2 μg of total RNA was used to synthesize double-stranded cDNA, then to produce biotin-tagged cRNA using MessageAmp™ II aRNA Amplification Kit.
Sample_hyb_protocol	Hybridization was performed at 45°C with rotation for 16 h (Affymetrix GeneChip Hybridization Oven 640). The GeneChip arrays were washed and then stained (streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000
Sample_data_processing	The hybridization data were analyzed using GeneChip Operating software (GCOS 1.4). The scanned images were firstly assessed by visual inspection then analyzed to generate raw data files saved as CEL files using the default setting of GCOS 1.4. A global scaling factor of 500 was used to normalize the different arrays.
Sample_platform_id	GPL570
Sample_contact_name	Yupeng,,Chen
Sample_contact_institute	Peking University Health Science Center
Sample_contact_address	Haidian District 38
Sample_contact_city	Beijing
Sample_contact_zip/postal_code	100083
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286136/suppl/GSM286136.CEL.gz
Sample_series_id	GSE11330
Sample_data_row_count	54675

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