Search results for the GEO ID: GSE11336  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM286229 | GPL570 |
|
C7H2-6h-GC-Exp0
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CCRF-CEM-C7H2 cell line
|
homo sapiens , C7H2 cell line is a GC-sensitive subclone of CCRF-CEM (ATCC no. CCL-119), cultured under standard conditions (37°C, 5% CO2, saturated humidity), in the presence of dexamethansone (a GC) 10e-7M for 6h.
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0929_001_Affy6hp0_h01_MB_230108.CEL
|
| Sample_geo_accession | GSM286229
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | May 05 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | cultured under standard conditions (37°C, 5% CO2, saturated humidity)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity by using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | R-Phycoerythrin
| | Sample_label_protocol_ch1 | The chips were stained and washed in an Affymetrix fluidic station 450 following the EukGE-ws2v4 protocol.
| | Sample_hyb_protocol | On rotation (60 rpm) hybridization at 45C for 16 hours
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | Raw signal intensities were preprocessed with the GCRMA algorithm. Preprocessing was performed in R (version 2.6) with Bioconductor's (version 2.1) gcrma package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286229/suppl/GSM286229.CEL.gz
| | Sample_series_id | GSE11336
| | Sample_data_row_count | 54675
| | |
|
GSM286230 | GPL570 |
|
C7H2-6h-GC-Exp1
|
CCRF-CEM-C7H2 cell line
|
homo sapiens , C7H2 cell line is a GC-sensitive subclone of CCRF-CEM (ATCC no. CCL-119), cultured under standard conditions (37°C, 5% CO2, saturated humidity), in the presence of dexamethasone (a GC) 10e-7M for 6h, experiment 1.
|
0930_001_Affy6hp1_h01_MB_230108.CEL
|
| Sample_geo_accession | GSM286230
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | May 05 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | cultured under standard conditions (37°C, 5% CO2, saturated humidity)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity by using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | R-Phycoerythrin
| | Sample_label_protocol_ch1 | The chips were stained and washed in an Affymetrix fluidic station 450 following the EukGE-ws2v4 protocol.
| | Sample_hyb_protocol | On rotation (60 rpm) hybridization at 45C for 16 hours
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | Raw signal intensities were preprocessed with the GCRMA algorithm. Preprocessing was performed in R (version 2.6) with Bioconductor's (version 2.1) gcrma package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286230/suppl/GSM286230.CEL.gz
| | Sample_series_id | GSE11336
| | Sample_data_row_count | 54675
| | |
|
GSM286231 | GPL570 |
|
C7H2-6h-GC-Exp3
|
CCRF-CEM-C7H2 cell line
|
homo sapiens , C7H2 cell line is a GC-sensitive subclone of CCRF-CEM (ATCC no. CCL-119), cultured under standard conditions (37°C, 5% CO2, saturated humidity), in the presence of dexamethasone (a GC) 10e-7M for 6h, experiment 3.
|
0931_001_Affy6hp3_h01_MB_230108.CEL
|
| Sample_geo_accession | GSM286231
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | May 05 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | cultured under standard conditions (37°C, 5% CO2, saturated humidity)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity by using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | R-Phycoerythrin
| | Sample_label_protocol_ch1 | The chips were stained and washed in an Affymetrix fluidic station 450 following the EukGE-ws2v4 protocol.
| | Sample_hyb_protocol | On rotation (60 rpm) hybridization at 45C for 16 hours
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | Raw signal intensities were preprocessed with the GCRMA algorithm. Preprocessing was performed in R (version 2.6) with Bioconductor's (version 2.1) gcrma package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286231/suppl/GSM286231.CEL.gz
| | Sample_series_id | GSE11336
| | Sample_data_row_count | 54675
| | |
|
GSM286232 | GPL570 |
|
C7H2-6h-EtOH-Exp0
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CCRF-CEM-C7H2 cell line
|
homo sapiens , C7H2 cell line is a GC-sensitive subclone of CCRF-CEM (ATCC no. CCL-119), cultured under standard conditions (37°C, 5% CO2, saturated humidity), treated for 6h with 0.1% ethanol (EtOH) as carrier control, experiment 0.
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0938_001_Affy6hm0_h01_MB130208.CEL
|
| Sample_geo_accession | GSM286232
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | May 05 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | cultured under standard conditions (37°C, 5% CO2, saturated humidity)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity by using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | R-Phycoerythrin
| | Sample_label_protocol_ch1 | The chips were stained and washed in an Affymetrix fluidic station 450 following the EukGE-ws2v4 protocol.
| | Sample_hyb_protocol | On rotation (60 rpm) hybridization at 45C for 16 hours
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | Raw signal intensities were preprocessed with the GCRMA algorithm. Preprocessing was performed in R (version 2.6) with Bioconductor's (version 2.1) gcrma package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286232/suppl/GSM286232.CEL.gz
| | Sample_series_id | GSE11336
| | Sample_data_row_count | 54675
| | |
|
GSM286233 | GPL570 |
|
C7H2-6h-EtOH-Exp1
|
CCRF-CEM-C7H2 cell line
|
homo sapiens , C7H2 cell line is a GC-sensitive subclone of CCRF-CEM (ATCC no. CCL-119), cultured under standard conditions (37°C, 5% CO2, saturated humidity), treated for 6h with 0.1% ethanol (EtOH) as carrier control, experiment 1.
|
0939_001_Affy6hm1_h01_MB130208.CEL
|
| Sample_geo_accession | GSM286233
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | May 05 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | cultured under standard conditions (37°C, 5% CO2, saturated humidity)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity by using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | R-Phycoerythrin
| | Sample_label_protocol_ch1 | The chips were stained and washed in an Affymetrix fluidic station 450 following the EukGE-ws2v4 protocol.
| | Sample_hyb_protocol | On rotation (60 rpm) hybridization at 45C for 16 hours
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | Raw signal intensities were preprocessed with the GCRMA algorithm. Preprocessing was performed in R (version 2.6) with Bioconductor's (version 2.1) gcrma package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286233/suppl/GSM286233.CEL.gz
| | Sample_series_id | GSE11336
| | Sample_data_row_count | 54675
| | |
|
GSM286234 | GPL570 |
|
C7H2-6h-EtOH-Exp3
|
CCRF-CEM-C7H2 cell line
|
homo sapiens , C7H2 cell line is a GC-sensitive subclone of CCRF-CEM (ATCC no. CCL-119), cultured under standard conditions (37°C, 5% CO2, saturated humidity), treated for 6h with 0.1% ethanol (EtOH) as carrier control, experiment 3.
|
0940_001_Affy6hm3_h01_MB_130208.CEL
|
| Sample_geo_accession | GSM286234
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | May 05 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | cultured under standard conditions (37°C, 5% CO2, saturated humidity)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity by using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | R-Phycoerythrin
| | Sample_label_protocol_ch1 | The chips were stained and washed in an Affymetrix fluidic station 450 following the EukGE-ws2v4 protocol.
| | Sample_hyb_protocol | On rotation (60 rpm) hybridization at 45C for 16 hours
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | Raw signal intensities were preprocessed with the GCRMA algorithm. Preprocessing was performed in R (version 2.6) with Bioconductor's (version 2.1) gcrma package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286234/suppl/GSM286234.CEL.gz
| | Sample_series_id | GSE11336
| | Sample_data_row_count | 54675
| | |
|
GSM286235 | GPL570 |
|
C7H2-24h-EtOH-Exp1
|
CCRF-CEM-C7H2 cell line
|
homo sapiens , C7H2 cell line is a GC-sensitive subclone of CCRF-CEM (ATCC no. CCL-119), cultured under standard conditions (37°C, 5% CO2, saturated humidity), treated for 24h with 0.1% ethanol (EtOH) as carrier control, experiment 1.
|
0941_001_C7H224m1_h01_MB_150208.CEL
|
| Sample_geo_accession | GSM286235
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | May 05 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | cultured under standard conditions (37°C, 5% CO2, saturated humidity)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity by using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | R-Phycoerythrin
| | Sample_label_protocol_ch1 | The chips were stained and washed in an Affymetrix fluidic station 450 following the EukGE-ws2v4 protocol.
| | Sample_hyb_protocol | On rotation (60 rpm) hybridization at 45C for 16 hours
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | Raw signal intensities were preprocessed with the GCRMA algorithm. Preprocessing was performed in R (version 2.6) with Bioconductor's (version 2.1) gcrma package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286235/suppl/GSM286235.CEL.gz
| | Sample_series_id | GSE11336
| | Sample_data_row_count | 54675
| | |
|
GSM286236 | GPL570 |
|
C7H2-24h-GC-Exp1
|
CCRF-CEM-C7H2 cell line
|
homo sapiens , C7H2 cell line is a GC-sensitive subclone of CCRF-CEM (ATCC no. CCL-119), cultured under standard conditions (37°C, 5% CO2, saturated humidity), in the presence of dexamethasone (a GC) 10e-7M for 24h, experiment 1.
|
0942_001_C7H224p1_h01_MB_150208.CEL
|
| Sample_geo_accession | GSM286236
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | May 05 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | cultured under standard conditions (37°C, 5% CO2, saturated humidity)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity by using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | R-Phycoerythrin
| | Sample_label_protocol_ch1 | The chips were stained and washed in an Affymetrix fluidic station 450 following the EukGE-ws2v4 protocol.
| | Sample_hyb_protocol | On rotation (60 rpm) hybridization at 45C for 16 hours
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | Raw signal intensities were preprocessed with the GCRMA algorithm. Preprocessing was performed in R (version 2.6) with Bioconductor's (version 2.1) gcrma package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286236/suppl/GSM286236.CEL.gz
| | Sample_series_id | GSE11336
| | Sample_data_row_count | 54675
| | |
|
GSM286237 | GPL570 |
|
C7H2-24h-EtOH-Exp2
|
CCRF-CEM-C7H2 cell line
|
homo sapiens , C7H2 cell line is a GC-sensitive subclone of CCRF-CEM (ATCC no. CCL-119), cultured under standard conditions (37°C, 5% CO2, saturated humidity), treated for 24h with 0.1% ethanol (EtOH) as carrier control, experiment 2.
|
0943_001_C7H224m2_h01_MB_150208.CEL
|
| Sample_geo_accession | GSM286237
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | May 05 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | cultured under standard conditions (37°C, 5% CO2, saturated humidity)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity by using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | R-Phycoerythrin
| | Sample_label_protocol_ch1 | The chips were stained and washed in an Affymetrix fluidic station 450 following the EukGE-ws2v4 protocol.
| | Sample_hyb_protocol | On rotation (60 rpm) hybridization at 45C for 16 hours
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | Raw signal intensities were preprocessed with the GCRMA algorithm. Preprocessing was performed in R (version 2.6) with Bioconductor's (version 2.1) gcrma package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286237/suppl/GSM286237.CEL.gz
| | Sample_series_id | GSE11336
| | Sample_data_row_count | 54675
| | |
|
GSM286238 | GPL570 |
|
C7H2-24h-GC-Exp2
|
CCRF-CEM-C7H2 cell line
|
homo sapiens , C7H2 cell line is a GC-sensitive subclone of CCRF-CEM (ATCC no. CCL-119), cultured under standard conditions (37°C, 5% CO2, saturated humidity), in the presence of dexamethasone (a GC) 10e-7M for 24h, experiment 2.
|
0944_001_C7H224p2_h01_MB_150208.CEL
|
| Sample_geo_accession | GSM286238
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | May 05 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | cultured under standard conditions (37°C, 5% CO2, saturated humidity)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity by using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | R-Phycoerythrin
| | Sample_label_protocol_ch1 | The chips were stained and washed in an Affymetrix fluidic station 450 following the EukGE-ws2v4 protocol.
| | Sample_hyb_protocol | On rotation (60 rpm) hybridization at 45C for 16 hours
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | Raw signal intensities were preprocessed with the GCRMA algorithm. Preprocessing was performed in R (version 2.6) with Bioconductor's (version 2.1) gcrma package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286238/suppl/GSM286238.CEL.gz
| | Sample_series_id | GSE11336
| | Sample_data_row_count | 54675
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GSM286239 | GPL570 |
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C7H2-24h-EtOH-Exp3
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CCRF-CEM-C7H2 cell line
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homo sapiens , C7H2 cell line is a GC-sensitive subclone of CCRF-CEM (ATCC no. CCL-119), cultured under standard conditions (37°C, 5% CO2, saturated humidity), treated for 24h with 0.1% ethanol (EtOH) as carrier control, experiment 3.
|
0945_001_C7H224m3_h01_MB_150208.CEL
|
| Sample_geo_accession | GSM286239
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | May 05 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | cultured under standard conditions (37°C, 5% CO2, saturated humidity)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity by using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | R-Phycoerythrin
| | Sample_label_protocol_ch1 | The chips were stained and washed in an Affymetrix fluidic station 450 following the EukGE-ws2v4 protocol.
| | Sample_hyb_protocol | On rotation (60 rpm) hybridization at 45C for 16 hours
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | Raw signal intensities were preprocessed with the GCRMA algorithm. Preprocessing was performed in R (version 2.6) with Bioconductor's (version 2.1) gcrma package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286239/suppl/GSM286239.CEL.gz
| | Sample_series_id | GSE11336
| | Sample_data_row_count | 54675
| | |
|
GSM286240 | GPL570 |
|
C7H2-24h-GC-Exp3
|
CCRF-CEM-C7H2 cell line
|
homo sapiens , C7H2 cell line is a GC-sensitive subclone of CCRF-CEM (ATCC no. CCL-119), cultured under standard conditions (37°C, 5% CO2, saturated humidity), in the presence of dexamethasone (a GC) 10e-7M for 24h, experiment 3.
|
0946_001_C7H224p3_h01_MB_150208.CEL
|
| Sample_geo_accession | GSM286240
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | May 05 2008
| | Sample_last_update_date | Jan 16 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | cultured under standard conditions (37°C, 5% CO2, saturated humidity)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | For total RNA isolation, TRIreagent (MRC Inc., Cincinnati, OH,USA) was used according to the manufacturer’s protocol. Briefly, up to 1x10E7 cells per ml were lysed with TRIreagent, 200ul of chloroform were added, the mixture centrifuged, and the RNA from the aqueous phase precipitated with isopropanol. The pelleted RNA was washed in 70% ethanol in DEPC-water and resuspended in nuclease-free water. RNA quantity and purity was determined by optical densitiy measurements (OD260/280) and RNA integrity by using the 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA), and only high quality RNA was further processed.
| | Sample_label_ch1 | R-Phycoerythrin
| | Sample_label_protocol_ch1 | The chips were stained and washed in an Affymetrix fluidic station 450 following the EukGE-ws2v4 protocol.
| | Sample_hyb_protocol | On rotation (60 rpm) hybridization at 45C for 16 hours
| | Sample_scan_protocol | Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
| | Sample_data_processing | Raw signal intensities were preprocessed with the GCRMA algorithm. Preprocessing was performed in R (version 2.6) with Bioconductor's (version 2.1) gcrma package.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Johannes,,Rainer
| | Sample_contact_email | johannes.rainer@i-med.ac.at
| | Sample_contact_laboratory | Applied Bioinformatics Group
| | Sample_contact_department | Biocenter, Division Molecular Pathophysiology
| | Sample_contact_institute | Medical University Innsbruck
| | Sample_contact_address | Innrain 80-82 II
| | Sample_contact_city | Innsbruck
| | Sample_contact_zip/postal_code | 6020
| | Sample_contact_country | Austria
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM286nnn/GSM286240/suppl/GSM286240.CEL.gz
| | Sample_series_id | GSE11336
| | Sample_data_row_count | 54675
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