Search results for the GEO ID: GSE11350  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM282008 | GPL570 |
|
Generation of pluripotent stem cells from adult human testis_hGS1
|
hGS cells from testis, cultivated with KO Medium+GDNF
|
hGS cells from testis, cultivated with KO Medium+GDNF
|
hGS cells from testis, cultivated with KO Medium+GDNF
|
| Sample_geo_accession | GSM282008
| | Sample_status | Public on May 09 2008
| | Sample_submission_date | Apr 17 2008
| | Sample_last_update_date | May 08 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Prof. Dr. Stenzl Department of Urology, Tuebingen University
| | Sample_growth_protocol_ch1 | Spermatogonial cells (hGS) from 3 different normal patients (patient 59, 73 and 81, passage 0) were cultured for 4 days in Knockout medium, 20% Knockout-FBS, 1% L-glutamine and 4ng/ml GDNF. After enrichment with CD49f and matrix selection with collagen and laminin the cells were washed with phosphate buffered saline (PBS) and pelleted at 1000rpm/10min./4°C, lysed with RLT buffer + ß-Mercaptoethanol and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | standard Qiagen Mini protocol
| | Sample_label_ch1 | Affymetrix standard protocol
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol with the GeneChip scanner 3000
| | Sample_data_processing | GC-RMA normalisation with ArrayAssist 5.1 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Bonin
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University Tuebingen
| | Sample_contact_address | CAlwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_state | BW
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282008/suppl/GSM282008.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282008/suppl/GSM282008.CHP.gz
| | Sample_series_id | GSE11350
| | Sample_data_row_count | 54675
| | |
|
GSM282009 | GPL570 |
|
Generation of pluripotent stem cells from adult human testis_ES1
|
H1 cells from testis, cultivated in ES Medium+FGF2
|
H1 cells from testis, cultivated with ES Medium+FGF2
|
H1 cells from testis, cultivated in ES Medium+FGF2
|
| Sample_geo_accession | GSM282009
| | Sample_status | Public on May 09 2008
| | Sample_submission_date | Apr 17 2008
| | Sample_last_update_date | May 08 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Inst. f. Neurophysiologie, Prof. J.Hescheler, Univ. Köln
| | Sample_growth_protocol_ch1 | hES(H1) cells (hES) were cultured for 10 days (passage 48, 57 and 67) in Knockout medium, 20% Knockout-Serum-Replacer, 1% L-glutamine, 1% NEAA, 1% penicillin/streptomycin and 4ng/ml FGF-2, on CF1 inactivated feeder on 0,1% gelatine coated dishes. After the clusters were manually cut, the isolated clusters were washed with phosphate buffered saline (PBS) and pelleted at 1000rpm/10min./4°C and lysed with RLT buffer + ß-Mercaptoethanol and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | standard Qiagen Mini protocol
| | Sample_label_ch1 | Affymetrix standard protocol
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol with the GeneChip scanner 3000
| | Sample_data_processing | GC-RMA normalisation with ArrayAssist 5.1 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Bonin
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University Tuebingen
| | Sample_contact_address | CAlwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_state | BW
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282009/suppl/GSM282009.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282009/suppl/GSM282009.CHP.gz
| | Sample_series_id | GSE11350
| | Sample_data_row_count | 54675
| | |
|
GSM282010 | GPL570 |
|
Generation of pluripotent stem cells from adult human testis_ES2
|
H1 cells from testis, cultivated in ES Medium+FGF2
|
H1 cells from testis, cultivated with ES Medium+FGF2
|
H1 cells from testis, cultivated in ES Medium+FGF2
|
| Sample_geo_accession | GSM282010
| | Sample_status | Public on May 09 2008
| | Sample_submission_date | Apr 17 2008
| | Sample_last_update_date | May 08 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Inst. f. Neurophysiologie, Prof. J.Hescheler, Univ. Köln
| | Sample_growth_protocol_ch1 | hES(H1) cells (hES) were cultured for 10 days (passage 48, 57 and 67) in Knockout medium, 20% Knockout-Serum-Replacer, 1% L-glutamine, 1% NEAA, 1% penicillin/streptomycin and 4ng/ml FGF-2, on CF1 inactivated feeder on 0,1% gelatine coated dishes. After the clusters were manually cut, the isolated clusters were washed with phosphate buffered saline (PBS) and pelleted at 1000rpm/10min./4°C and lysed with RLT buffer + ß-Mercaptoethanol and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | standard Qiagen Mini protocol
| | Sample_label_ch1 | Affymetrix standard protocol
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol with the GeneChip scanner 3000
| | Sample_data_processing | GC-RMA normalisation with ArrayAssist 5.1 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Bonin
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University Tuebingen
| | Sample_contact_address | CAlwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_state | BW
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282010/suppl/GSM282010.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282010/suppl/GSM282010.CHP.gz
| | Sample_series_id | GSE11350
| | Sample_data_row_count | 54675
| | |
|
GSM282011 | GPL570 |
|
Generation of pluripotent stem cells from adult human testis_ES3
|
H1 cells from testis, cultivated in ES Medium+FGF2
|
H1 cells from testis. cultivated with ES Medium+FGF2
|
H1 cells from testis, cultivated in ES Medium+FGF2
|
| Sample_geo_accession | GSM282011
| | Sample_status | Public on May 09 2008
| | Sample_submission_date | Apr 17 2008
| | Sample_last_update_date | May 08 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Inst. f. Neurophysiologie, Prof. J.Hescheler, Univ. Köln
| | Sample_growth_protocol_ch1 | hES(H1) cells (hES) were cultured for 10 days (passage 48, 57 and 67) in Knockout medium, 20% Knockout-Serum-Replacer, 1% L-glutamine, 1% NEAA, 1% penicillin/streptomycin and 4ng/ml FGF-2, on CF1 inactivated feeder on 0,1% gelatine coated dishes. After the clusters were manually cut, the isolated clusters were washed with phosphate buffered saline (PBS) and pelleted at 1000rpm/10min./4°C and lysed with RLT buffer + ß-Mercaptoethanol and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | standard Qiagen Mini protocol
| | Sample_label_ch1 | Affymetrix standard protocol
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol with the GeneChip scanner 3000
| | Sample_data_processing | GC-RMA normalisation with ArrayAssist 5.1 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Bonin
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University Tuebingen
| | Sample_contact_address | CAlwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_state | BW
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282011/suppl/GSM282011.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282011/suppl/GSM282011.CHP.gz
| | Sample_series_id | GSE11350
| | Sample_data_row_count | 54675
| | |
|
GSM282012 | GPL570 |
|
Generation of pluripotent stem cells from adult human testis_hGS2
|
hGS cells from testis, cultivated with KO Medium+GDNF
|
hGS cells from testis, cultivated with KO Medium+GDNF
|
hGS cells from testis, cultivated with KO Medium+GDNF
|
| Sample_geo_accession | GSM282012
| | Sample_status | Public on May 09 2008
| | Sample_submission_date | Apr 17 2008
| | Sample_last_update_date | May 08 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Prof. Dr. Stenzl Department of Urology, Tuebingen University
| | Sample_growth_protocol_ch1 | Spermatogonial cells (hGS) from 3 different normal patients (patient 59, 73 and 81, passage 0) were cultured for 4 days in Knockout medium, 20% Knockout-FBS, 1% L-glutamine and 4ng/ml GDNF. After enrichment with CD49f and matrix selection with collagen and laminin the cells were washed with phosphate buffered saline (PBS) and pelleted at 1000rpm/10min./4°C, lysed with RLT buffer + ß-Mercaptoethanol and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | standard Qiagen Mini protocol
| | Sample_label_ch1 | Affymetrix standard protocol
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol with the GeneChip scanner 3000
| | Sample_data_processing | GC-RMA normalisation with ArrayAssist 5.1 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Bonin
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University Tuebingen
| | Sample_contact_address | CAlwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_state | BW
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282012/suppl/GSM282012.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282012/suppl/GSM282012.CHP.gz
| | Sample_series_id | GSE11350
| | Sample_data_row_count | 54675
| | |
|
GSM282013 | GPL570 |
|
Generation of pluripotent stem cells from adult human testis_hGS3
|
hGS cells from testis, cultivated with KO Medium+GDNF
|
hGS cells from testis, cultivated with KO Medium+GDNF
|
hGS cells from testis, cultivated with KO Medium+GDNF
|
| Sample_geo_accession | GSM282013
| | Sample_status | Public on May 09 2008
| | Sample_submission_date | Apr 17 2008
| | Sample_last_update_date | May 08 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Prof. Dr. Stenzl Department of Urology, Tuebingen University
| | Sample_growth_protocol_ch1 | Spermatogonial cells (hGS) from 3 different normal patients (patient 59, 73 and 81, passage 0) were cultured for 4 days in Knockout medium, 20% Knockout-FBS, 1% L-glutamine and 4ng/ml GDNF. After enrichment with CD49f and matrix selection with collagen and laminin the cells were washed with phosphate buffered saline (PBS) and pelleted at 1000rpm/10min./4°C, lysed with RLT buffer + ß-Mercaptoethanol and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | standard Qiagen Mini protocol
| | Sample_label_ch1 | Affymetrix standard protocol
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol with the GeneChip scanner 3000
| | Sample_data_processing | GC-RMA normalisation with ArrayAssist 5.1 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Bonin
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University Tuebingen
| | Sample_contact_address | CAlwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_state | BW
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282013/suppl/GSM282013.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282013/suppl/GSM282013.CHP.gz
| | Sample_series_id | GSE11350
| | Sample_data_row_count | 54675
| | |
|
GSM282014 | GPL570 |
|
Generation of pluripotent stem cells from adult human testis_haGSC4
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
| Sample_geo_accession | GSM282014
| | Sample_status | Public on May 09 2008
| | Sample_submission_date | Apr 17 2008
| | Sample_last_update_date | May 08 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Prof. Dr. Stenzl Department of Urology, Tuebingen University
| | Sample_growth_protocol_ch1 | human adult germ stem cells (haGSCs) were derived from hGS cells from 3 different normal patients after the enrichment and further cultivation in LIF supplemented medium. haGSC from patient 52, 54 and 35 (from lower and higher passages) were cultured for 10 days in basic medium (DMEM high glucose, 15% FCS (Biochrom), 1% non-essential amino acids (NEAA), 1% L-glutamine and 0.05 mM ß-mercaptoethanol (Gibco) with 103 units/ml leukemia inhibitory factor (LIF, human, Chemicon) on 0,1% gelatine coated dishes. After the clusters were manually cut, the isolated clusters were washed with phosphate buffered saline (PBS) and pelleted at 1000rpm/10min./4°C and lysed with RLT buffer + ß-Mercaptoethanol and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | standard Qiagen Mini protocol
| | Sample_label_ch1 | Affymetrix standard protocol
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol with the GeneChip scanner 3000
| | Sample_data_processing | GC-RMA normalisation with ArrayAssist 5.1 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Bonin
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University Tuebingen
| | Sample_contact_address | CAlwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_state | BW
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282014/suppl/GSM282014.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282014/suppl/GSM282014.CHP.gz
| | Sample_series_id | GSE11350
| | Sample_data_row_count | 54675
| | |
|
GSM282015 | GPL570 |
|
Generation of pluripotent stem cells from adult human testis_haGSC5
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
| Sample_geo_accession | GSM282015
| | Sample_status | Public on May 09 2008
| | Sample_submission_date | Apr 17 2008
| | Sample_last_update_date | May 08 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Prof. Dr. Stenzl Department of Urology, Tuebingen University
| | Sample_growth_protocol_ch1 | human adult germ stem cells (haGSCs) were derived from hGS cells from 3 different normal patients after the enrichment and further cultivation in LIF supplemented medium. haGSC from patient 52, 54 and 35 (from lower and higher passages) were cultured for 10 days in basic medium (DMEM high glucose, 15% FCS (Biochrom), 1% non-essential amino acids (NEAA), 1% L-glutamine and 0.05 mM ß-mercaptoethanol (Gibco) with 103 units/ml leukemia inhibitory factor (LIF, human, Chemicon) on 0,1% gelatine coated dishes. After the clusters were manually cut, the isolated clusters were washed with phosphate buffered saline (PBS) and pelleted at 1000rpm/10min./4°C and lysed with RLT buffer + ß-Mercaptoethanol and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | standard Qiagen Mini protocol
| | Sample_label_ch1 | Affymetrix standard protocol
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol with the GeneChip scanner 3000
| | Sample_data_processing | GC-RMA normalisation with ArrayAssist 5.1 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Bonin
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University Tuebingen
| | Sample_contact_address | CAlwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_state | BW
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282015/suppl/GSM282015.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282015/suppl/GSM282015.CHP.gz
| | Sample_series_id | GSE11350
| | Sample_data_row_count | 54675
| | |
|
GSM282016 | GPL570 |
|
Generation of pluripotent stem cells from adult human testis_haGSC6
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
| Sample_geo_accession | GSM282016
| | Sample_status | Public on May 09 2008
| | Sample_submission_date | Apr 17 2008
| | Sample_last_update_date | May 08 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Prof. Dr. Stenzl Department of Urology, Tuebingen University
| | Sample_growth_protocol_ch1 | human adult germ stem cells (haGSCs) were derived from hGS cells from 3 different normal patients after the enrichment and further cultivation in LIF supplemented medium. haGSC from patient 52, 54 and 35 (from lower and higher passages) were cultured for 10 days in basic medium (DMEM high glucose, 15% FCS (Biochrom), 1% non-essential amino acids (NEAA), 1% L-glutamine and 0.05 mM ß-mercaptoethanol (Gibco) with 103 units/ml leukemia inhibitory factor (LIF, human, Chemicon) on 0,1% gelatine coated dishes. After the clusters were manually cut, the isolated clusters were washed with phosphate buffered saline (PBS) and pelleted at 1000rpm/10min./4°C and lysed with RLT buffer + ß-Mercaptoethanol and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | standard Qiagen Mini protocol
| | Sample_label_ch1 | Affymetrix standard protocol
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol with the GeneChip scanner 3000
| | Sample_data_processing | GC-RMA normalisation with ArrayAssist 5.1 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Bonin
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University Tuebingen
| | Sample_contact_address | CAlwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_state | BW
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282016/suppl/GSM282016.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282016/suppl/GSM282016.CHP.gz
| | Sample_series_id | GSE11350
| | Sample_data_row_count | 54675
| | |
|
GSM282017 | GPL570 |
|
Generation of pluripotent stem cells from adult human testis_haGSC1
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
| Sample_geo_accession | GSM282017
| | Sample_status | Public on May 09 2008
| | Sample_submission_date | Apr 17 2008
| | Sample_last_update_date | May 08 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Prof. Dr. Stenzl Department of Urology, Tuebingen University
| | Sample_growth_protocol_ch1 | human adult germ stem cells (haGSCs) were derived from hGS cells from 3 different normal patients after the enrichment and further cultivation in LIF supplemented medium. haGSC from patient 52, 54 and 35 (from lower and higher passages) were cultured for 10 days in basic medium (DMEM high glucose, 15% FCS (Biochrom), 1% non-essential amino acids (NEAA), 1% L-glutamine and 0.05 mM ß-mercaptoethanol (Gibco) with 103 units/ml leukemia inhibitory factor (LIF, human, Chemicon) on 0,1% gelatine coated dishes. After the clusters were manually cut, the isolated clusters were washed with phosphate buffered saline (PBS) and pelleted at 1000rpm/10min./4°C and lysed with RLT buffer + ß-Mercaptoethanol and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | standard Qiagen Mini protocol
| | Sample_label_ch1 | Affymetrix standard protocol
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol with the GeneChip scanner 3000
| | Sample_data_processing | GC-RMA normalisation with ArrayAssist 5.1 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Bonin
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University Tuebingen
| | Sample_contact_address | CAlwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_state | BW
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282017/suppl/GSM282017.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282017/suppl/GSM282017.CHP.gz
| | Sample_series_id | GSE11350
| | Sample_data_row_count | 54675
| | |
|
GSM282018 | GPL570 |
|
Generation of pluripotent stem cells from adult human testis_haGSC2
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
| Sample_geo_accession | GSM282018
| | Sample_status | Public on May 09 2008
| | Sample_submission_date | Apr 17 2008
| | Sample_last_update_date | May 08 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Prof. Dr. Stenzl Department of Urology, Tuebingen University
| | Sample_growth_protocol_ch1 | human adult germ stem cells (haGSCs) were derived from hGS cells from 3 different normal patients after the enrichment and further cultivation in LIF supplemented medium. haGSC from patient 52, 54 and 35 (from lower and higher passages) were cultured for 10 days in basic medium (DMEM high glucose, 15% FCS (Biochrom), 1% non-essential amino acids (NEAA), 1% L-glutamine and 0.05 mM ß-mercaptoethanol (Gibco) with 103 units/ml leukemia inhibitory factor (LIF, human, Chemicon) on 0,1% gelatine coated dishes. After the clusters were manually cut, the isolated clusters were washed with phosphate buffered saline (PBS) and pelleted at 1000rpm/10min./4°C and lysed with RLT buffer + ß-Mercaptoethanol and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | standard Qiagen Mini protocol
| | Sample_label_ch1 | Affymetrix standard protocol
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol with the GeneChip scanner 3000
| | Sample_data_processing | GC-RMA normalisation with ArrayAssist 5.1 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Bonin
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University Tuebingen
| | Sample_contact_address | CAlwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_state | BW
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282018/suppl/GSM282018.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282018/suppl/GSM282018.CHP.gz
| | Sample_series_id | GSE11350
| | Sample_data_row_count | 54675
| | |
|
GSM282019 | GPL570 |
|
Generation of pluripotent stem cells from adult human testis_haGSC3
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
haGSC cells from testis, cultivated with KO Medium+GDNF
|
| Sample_geo_accession | GSM282019
| | Sample_status | Public on May 09 2008
| | Sample_submission_date | Apr 17 2008
| | Sample_last_update_date | May 08 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Prof. Dr. Stenzl Department of Urology, Tuebingen University
| | Sample_growth_protocol_ch1 | human adult germ stem cells (haGSCs) were derived from hGS cells from 3 different normal patients after the enrichment and further cultivation in LIF supplemented medium. haGSC from patient 52, 54 and 35 (from lower and higher passages) were cultured for 10 days in basic medium (DMEM high glucose, 15% FCS (Biochrom), 1% non-essential amino acids (NEAA), 1% L-glutamine and 0.05 mM ß-mercaptoethanol (Gibco) with 103 units/ml leukemia inhibitory factor (LIF, human, Chemicon) on 0,1% gelatine coated dishes. After the clusters were manually cut, the isolated clusters were washed with phosphate buffered saline (PBS) and pelleted at 1000rpm/10min./4°C and lysed with RLT buffer + ß-Mercaptoethanol and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | standard Qiagen Mini protocol
| | Sample_label_ch1 | Affymetrix standard protocol
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol with the GeneChip scanner 3000
| | Sample_data_processing | GC-RMA normalisation with ArrayAssist 5.1 software
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Bonin
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University Tuebingen
| | Sample_contact_address | CAlwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_state | BW
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282019/suppl/GSM282019.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM282nnn/GSM282019/suppl/GSM282019.CHP.gz
| | Sample_series_id | GSE11350
| | Sample_data_row_count | 54675
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
